BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,293,160	G→A	noncoding (55/132 nt)	CXP41_RS06840 ←	RtT sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,293,160	0	G	A	89.6%	375.5 / 12.8	158	noncoding (55/132 nt)	CXP41_RS06840	RtT sRNA
Reads supporting (aligned to +/- strand): ref base G (9/7); new base A (108/30); total (120/38)
Fisher's exact test for biased strand distribution p-value = 6.51e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

Only 100 of 158 total aligned reads displayed.

GAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAT  >  NZ_CP025268/1293075‑1293297

                           aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGtt                                                          >  2:2429907/1‑140 (MQ=12)

                            cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTc                                                         <  2:3040719/140‑1 (MQ=17)

                            cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTc                                                         >  1:4457675/1‑140 (MQ=16)

                            cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTc                                                         >  2:2839876/1‑140 (MQ=12)

                            cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTc                                                         <  2:1609874/140‑1 (MQ=16)

                            cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTc                                                         <  2:1610109/140‑1 (MQ=16)

                              cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAg                                                       <  2:3094538/140‑1 (MQ=19)

                                gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAggg                                                     <  2:3674931/140‑1 (MQ=255)

                                gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAggg                                                     <  1:2478435/140‑1 (MQ=255)

                                 cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGGGAGTTCAGGGa                                                    >  1:3818752/1‑140 (MQ=255)

                                 cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGGGAGTTCAGGGa                                                    >  2:4760132/1‑140 (MQ=255)

                                 cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                    >  1:3654639/1‑140 (MQ=255)

                                 cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                    >  1:1020360/1‑140 (MQ=255)

                                 cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                    >  2:1714616/1‑140 (MQ=255)

                                 cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATGTGATTGCGGTTTGGTTTGAGAGTTCAgggg                                                    >  2:842343/1‑139 (MQ=11)

                                  gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGAc                                                   >  1:741700/1‑140 (MQ=255)

                                    tGCGCGCTTCGCGCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtt                                                 >  2:5137492/1‑140 (MQ=255)

                                    tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGGGAGTTCAGGGACtt                                                 >  1:3303895/1‑140 (MQ=255)

                                      cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                               >  1:396909/1‑140 (MQ=37)

                                      cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                               <  2:589860/140‑1 (MQ=255)

                                      cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATTTTTGATTGCGGATTCGCTGGCGAGTTCAGGGACtttt                                               >  2:1387913/1‑140 (MQ=11)

                                      cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                               <  2:191579/140‑1 (MQ=255)

                                      cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                               >  1:2086972/1‑140 (MQ=255)

                                       gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGTCAGAACATTTGATTGCGGATTCACGTGAGAGCTCCGCGACtttgg                                              >  1:823858/1‑138 (MQ=11)

                                         gcgtCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCTCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                            <  1:1992612/137‑1 (MQ=255)

                                         gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATCGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                            <  2:782059/140‑1 (MQ=255)

                                          cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                           <  1:3142807/140‑1 (MQ=255)

                                           ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTGTCTCGCTGACGCTCGAGTCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg                                          <  2:828742/140‑1 (MQ=255)

                                           ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAGTTCAGGGGCTTTTGAAAg                                          >  1:4493910/1‑140 (MQ=11)

                                           ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg                                          >  1:4978079/1‑140 (MQ=255)

                                           ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg                                          >  1:2758092/1‑140 (MQ=255)

                                           ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTGCAGGGACTTTTGAAAg                                          >  1:25177/1‑140 (MQ=255)

                                           ttCGCCCTTCGGGTCGTTGCCTGCGACCACGCTCTCTCGCTCACCCTCGAGCGGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg                                          >  2:4665019/1‑140 (MQ=14)

                                             cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTg                                        >  1:3598811‑M2/1‑139 (MQ=255)

                                             cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTg                                        >  1:9260‑M2/1‑139 (MQ=255)

                                              gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTga                                       >  1:4385039‑M2/1‑138 (MQ=255)

                                              gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTga                                       >  1:4002669‑M2/1‑138 (MQ=255)

                                               cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgat                                      >  1:536250‑M2/1‑137 (MQ=255)

                                                ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatg                                     >  1:672729‑M2/1‑136 (MQ=255)

                                                ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatg                                     >  2:1131027‑M2/1‑136 (MQ=255)

                                                ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatg                                     >  1:4828340‑M2/1‑136 (MQ=255)

                                                 cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatgg                                    >  1:160255‑M2/1‑135 (MQ=255)

                                                  ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggt                                   >  2:4195139‑M2/1‑134 (MQ=255)

                                                  ttCGGGGCGTTGCGTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAAACTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggt                                   >  2:4195198‑M2/1‑134 (MQ=255)

                                                    cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtgg                                 >  2:2355320‑M2/1‑132 (MQ=255)

                                                    cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtgg                                 >  2:4285929‑M2/1‑132 (MQ=255)

                                                    cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtgg                                 >  2:4288067‑M2/1‑132 (MQ=255)

                                                    cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtgg                                 >  1:288189‑M2/1‑132 (MQ=255)

                                                    cGGGTCGTTGCCGGCGGCACCGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCCAAGCTTCTCATCCTTCCCCGCATGGGCAGACTATTTGATTGCGGCTTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtgg                                 >  1:2328945‑M2/1‑132 (MQ=255)

                                                     ggggCGGTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGGCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGTTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggt                                >  2:4465504‑M2/1‑131 (MQ=255)

                                                     gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggt                                >  2:1228261‑M2/1‑131 (MQ=255)

                                                       gTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAGTTCCGGGACTTTTGAAAGTgatggtggtgg                              <  2:1980765‑M2/140‑12 (MQ=255)

                                                        tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggg                             >  1:522627‑M2/1‑128 (MQ=255)

                                                        tCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggg                             >  1:1490002‑M2/1‑128 (MQ=255)

                                                        tCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggg                             >  1:1062012‑M2/1‑128 (MQ=255)

                                                          ggtgcctgccgcAACGCTCTCTCGCTGACGCGCGAGCTGAACATTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                           >  2:2096565‑M2/3‑126 (MQ=255)

                                                          ggtgcCTGCGGCATCGCTCTCTCGCTGACGCTCGAGCCGAACGTTAGTCGTAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCCGGGCCTTTTGAAAGTgatggtggtggggg                           >  2:3160035‑M2/3‑126 (MQ=255)

                                                          gTTGCCTGCGGCAACGTTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                           >  2:3119068‑M2/1‑126 (MQ=255)

                                                          gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGGACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                           >  2:2281532‑M2/1‑126 (MQ=255)

                                                          gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGGGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                           <  2:2454030‑M2/140‑15 (MQ=255)

                                                          gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGCGTTCAGGGACTTTTGAAAGTgatggtggttgggg                           <  1:2269777‑M2/140‑15 (MQ=255)

                                                          gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                           >  1:367950‑M2/1‑126 (MQ=255)

                                                          gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                           >  2:3950431‑M2/1‑126 (MQ=255)

                                                          gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                           >  1:4755016‑M2/1‑126 (MQ=255)

                                                          gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                           >  2:2047624‑M2/1‑126 (MQ=255)

                                                          gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                           >  1:2174720‑M2/1‑126 (MQ=255)

                                                          gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                           >  1:746774‑M2/1‑126 (MQ=255)

                                                          gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtgg                                 >  1:4481430‑M2/1‑126 (MQ=255)

                                                          gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTGGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                           <  2:2295134‑M2/140‑15 (MQ=255)

                                                          gTTGCCTGCGGCAACGCTCACTCGCTGACGCTCGGGCCGAACCTTCGGCGAGGCTTCTTACCTTTCCGCGCATGGGCAGACTATTTGATTGCCGATTCGTTAGAGAGTGCAGGCACTTTTGGAAGTGATGGTGGTggggg                           <  2:4128022/140‑1 (MQ=2)

                                                          gTTGCCCGCCGCAACGCTCTCTCGCTGCCGCGCGAGCCGTATCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                           >  2:1693985‑M2/1‑126 (MQ=255)

                                                               cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                      >  2:5172256‑M2/1‑121 (MQ=255)

                                                               cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                      >  2:1940352‑M2/1‑121 (MQ=255)

                                                               cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                      >  2:2806659‑M2/1‑121 (MQ=255)

                                                               cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                      >  1:1922312‑M2/1‑121 (MQ=255)

                                                               cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                      >  2:3591391‑M2/1‑121 (MQ=255)

                                                               cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                      >  2:31631‑M2/1‑121 (MQ=255)

                                                               cTGCGGCAACGCTCTCTCGCTGACGATCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                      >  1:2741601‑M2/1‑121 (MQ=255)

                                                               cTGAGGCAACGCTCTCTCGTTGAGGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATGTTTGATTGGGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                      >  1:4809815‑M2/1‑121 (MQ=255)

                                                                  cGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggatta                   <  1:2943552‑M2/140‑23 (MQ=255)

                                                                    gtaacgCTCTTTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattact                 >  1:911112‑M2/3‑116 (MQ=255)

                                                                    gCAACGCTCTCTCTCTGATGCTCCGGCGGACACTTAGTTGTAGCCTATCCTCCTCCCCCGCATGGGCAGATGAGTTTAAGGCGCATGCGTTTGAGGGTTCGGTTACTTTTGGAAGCGATGGTGGTGGGGGAAGGAttact                 <  2:4879071/140‑4 (MQ=0)

                                                                    gCAACGCTCTCTCGCTGGCGCTCGAGTCGAACTTTGTGCGAAGGTTATCATCCTGCTCTGCACGGGCAGAATAGTTGGCTGCGGATGCGGTGGGTAGGTCAGGGACTTTTGGAAGTGATGGTGGTGGGGGAAGGATTCGa                 <  2:583964/140‑1 (MQ=2)

                                                                    gCAACGCTCTCTCGCTGACGCTCGGGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCGGCATGGGCAGAAGAGTTGATTGCGGATGCGCTTGAGAGTTCGGGGACTTTTGAAAGTgatggtggtgggggaaggattact                 >  2:3963947‑M2/1‑116 (MQ=255)

                                                                    gCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGATTCTCATCTTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGTACTTTTGGAAGTgatggtggtgggggaaggattact                 >  2:4879051‑M2/1‑116 (MQ=255)

                                                                     cAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactc                <  1:4877173‑M2/140‑26 (MQ=255)

                                                                     cAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactc                <  1:1119850‑M2/140‑26 (MQ=255)

                                                                     cAACCCTCTCTCTCCGACGCTCGCGCCGTACCTTAGTCTAAGCTTCTCATCCTTCACCGCCTGGGCCGAGTATTTGATTGCGGATTCGCTTGGCCGTTCAGGGACTTGTGAAAGTGATCGCGGTGGGGGAAGGAttactt                >  2:5153711/1‑136 (MQ=2)

                                                                        cGCTCTCTCGCTGGCGCTCAAGTCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagc             <  1:4537464‑M2/140‑29 (MQ=255)

                                                                        cGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagc             <  1:33402‑M2/140‑29 (MQ=255)

                                                                        cGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagc             <  1:3886711‑M2/140‑29 (MQ=255)

                                                                         gCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcg            >  1:2005642‑M2/1‑111 (MQ=255)

                                                                         gCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcg            >  1:1871494‑M2/1‑111 (MQ=255)

                                                                           tctctcGCTGACGCTCGAGCCGAAACTTAGTCGAAGCTTATCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgct          >  1:487141‑M2/1‑109 (MQ=255)

                                                                            ctctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctg         >  1:1714616‑M2/1‑108 (MQ=255)

                                                                            ctctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctg         >  1:832436‑M2/1‑108 (MQ=255)

                                                                            ctctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctg         >  1:4798806‑M2/1‑108 (MQ=255)

                                                                             tctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgc        >  1:4429797‑M2/1‑107 (MQ=255)

                                                                             tctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgc        <  1:2807821‑M2/140‑34 (MQ=255)

                                                                                   tGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgctt  >  1:286340‑M2/1‑101 (MQ=255)

GAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAT  >  NZ_CP025268/1293075‑1293297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`