BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,293,179	A→G	noncoding (36/132 nt)	CXP41_RS06840 ←	RtT sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,293,179	0	A	G	99.4%	545.6 / NA	180	noncoding (36/132 nt)	CXP41_RS06840	RtT sRNA
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (129/50); minor base T (0/1); total (129/51)
Fisher's exact test for biased strand distribution p-value = 2.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

ACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAG  >  NZ_CP025268/1293069‑1293313

acttaagAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAGCCTTCCCCGCATggg                                                                                                            <  2:4261294/134‑1 (MQ=11)

                                tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGGCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATTGGCAGAATATTAGATTGCGGATTCGCTTGGGA‑Gt                                                                           >  1:4871072/1‑140 (MQ=11)

                                  cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTc                                                                         <  2:3040719/140‑1 (MQ=17)

                                  cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTc                                                                         <  2:1609874/140‑1 (MQ=16)

                                  cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTc                                                                         >  1:4457675/1‑140 (MQ=16)

                                  cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTc                                                                         >  2:2839876/1‑140 (MQ=12)

                                  cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGCTTGAGA‑GTTc                                                                         >  2:2795158/1‑140 (MQ=12)

                                    cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCCACGCTCTCTCGCTGGCGCTCAAGTCGAACCTCAGGCGAAGCTTCTCATCCTTCCCCGCCTGGTCAGAATATTTGATTGCGGATTCGTTTGATA‑GTTCAg                                                                       <  2:3094616/140‑1 (MQ=11)

                                       cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGTTTGAGA‑GTTCAgggg                                                                    >  2:2277109/1‑139 (MQ=11)

                                       cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGTGCCGAACCTTAGCCGCAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGa                                                                    >  2:3922131/1‑140 (MQ=11)

                                       cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGGGA‑GTTCAGGGa                                                                    >  1:3818752/1‑140 (MQ=255)

                                       cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGa                                                                    >  1:1020360/1‑140 (MQ=255)

                                       cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGa                                                                    >  2:1714616/1‑140 (MQ=255)

                                       cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAACTTTTGATTGCGCATTCGGTTGACA‑GCTCCGGGa                                                                    >  2:1774574/1‑140 (MQ=11)

                                       cgcTACGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTCACTTGCGGATTCGCTTGACA‑GTTCAgggg                                                                    >  2:4805963/1‑139 (MQ=11)

                                        gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGAc                                                                   >  1:741748/1‑140 (MQ=255)

                                          tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGGGA‑GTTCAGGGACtt                                                                 >  1:3303895/1‑140 (MQ=255)

                                          tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACtt                                                                 >  1:4615172/1‑140 (MQ=255)

                                            cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACtttt                                                               <  2:589860/140‑1 (MQ=255)

                                            cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATTTTTGATTGCGGATTCGCTGGCGA‑GTTCAGGGACtttt                                                               >  2:1387913/1‑140 (MQ=11)

                                            cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGATA‑GTTCAGGGACtttt                                                               >  2:1959156/1‑140 (MQ=255)

                                            cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACtttt                                                               >  1:1584952/1‑140 (MQ=255)

                                            cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACtttt                                                               <  2:191579/140‑1 (MQ=255)

                                            cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACtttt                                                               >  1:4801342/1‑140 (MQ=255)

                                            cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACtttt                                                               >  1:396356/1‑140 (MQ=255)

                                            cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACtttt                                                               >  1:2086972/1‑140 (MQ=255)

                                            cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTGGAGA‑GTTCAGGGACtttt                                                               >  1:368431/1‑140 (MQ=255)

                                            cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCGTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACtttt                                                               >  1:541124/1‑140 (MQ=255)

                                             gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGTCAGAACATTTGATTGCGGATTCACGTGAGA‑GCTCCGCGACtttgg                                                              >  1:823858/1‑138 (MQ=11)

                                               gcgtCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCTCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGaa                                                            <  1:1992612/137‑1 (MQ=255)

                                               gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATCGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGaa                                                            <  2:782059/140‑1 (MQ=255)

                                                cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGaaa                                                           <  1:3142807/140‑1 (MQ=255)

                                                 ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGGCTTTTGAAAg                                                          >  1:3643234/1‑140 (MQ=255)

                                                 ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAg                                                          >  2:4385039/1‑140 (MQ=255)

                                                 ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAg                                                          >  1:2758092/1‑140 (MQ=255)

                                                 ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTGCAGGGACTTTTGAAAg                                                          >  1:25177/1‑140 (MQ=255)

                                                    gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTga                                                       >  1:4465049‑M2/1‑138 (MQ=255)

                                                    gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTga                                                       >  1:4385039‑M2/1‑138 (MQ=255)

                                                    gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTga                                                       >  1:4002669‑M2/1‑138 (MQ=255)

                                                      ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatg                                                     >  2:1131027‑M2/1‑136 (MQ=255)

                                                      ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatg                                                     >  1:672729‑M2/1‑136 (MQ=255)

                                                        ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggt                                                   >  2:4195139‑M2/1‑134 (MQ=255)

                                                         tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtg                                                  >  1:2900057‑M2/1‑133 (MQ=255)

                                                          cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtgg                                                 >  2:2356598‑M2/1‑132 (MQ=255)

                                                          cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtgg                                                 >  2:2355320‑M2/1‑132 (MQ=255)

                                                          cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtgg                                                 >  2:4285929‑M2/1‑132 (MQ=255)

                                                          cGGGTCGTTGCCGGCGGCACCGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCCAAGCTTCTCATCCTTCCCCGCATGGGCAGACTATTTGATTGCGGCTTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtgg                                                 >  1:2328945‑M2/1‑132 (MQ=255)

                                                           gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggt                                                >  2:1228261‑M2/1‑131 (MQ=255)

                                                              tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtggg                                             >  1:522627‑M2/1‑128 (MQ=255)

                                                              tCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtggg                                             >  1:1062012‑M2/1‑128 (MQ=255)

                                                                ggtgcctgccgcAACGCTCTCTCGCTGACGCGCGAGCTGAACATTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtggggg                                           >  2:2096565‑M2/3‑126 (MQ=255)

                                                                ggtgcCTGCGGCATCGCTCTCTCGCTGACGCTCGAGCCGAACGTTAGTCGTAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCCGGGCCTTTTGAAAGTgatggtggtggggg                                           >  2:3160035‑M2/3‑126 (MQ=255)

                                                                gTTGCCTTCGACAACGCTCTCTCTCTGCCGCTCGAGCCGTACCTTAGTCGCAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtggggg                                           >  2:1693907‑M2/1‑126 (MQ=255)

                                                                gTTGCCTGCGGCAACGTTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtggggg                                           >  2:3119068‑M2/1‑126 (MQ=255)

                                                                gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGTCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtggggg                                           >  1:1892051‑M2/1‑126 (MQ=255)

                                                                gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGGGA‑GTTCAGGGACTTTTGAAAGTgatggtggtggggg                                           <  2:2454030‑M2/140‑15 (MQ=255)

                                                                gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGC‑GTTCAGGGACTTTTGAAAGTgatggtggttgggg                                           <  1:2269777‑M2/140‑15 (MQ=255)

                                                                gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtggggg                                           >  2:2047624‑M2/1‑126 (MQ=255)

                                                                gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtggggg                                           >  2:3950431‑M2/1‑126 (MQ=255)

                                                                gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtggggg                                           >  1:750065‑M2/1‑126 (MQ=255)

                                                                gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtggggg                                           >  1:2174720‑M2/1‑126 (MQ=255)

                                                                gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtggggg                                           >  1:5126468‑M2/1‑126 (MQ=255)

                                                                gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtggggg                                           >  1:4954295‑M2/1‑126 (MQ=255)

                                                                gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtggggg                                           >  1:367950‑M2/1‑126 (MQ=255)

                                                                gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtggggg                                           >  1:4755016‑M2/1‑126 (MQ=255)

                                                                gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtgg                                                 <  2:4481430‑M2/134‑9 (MQ=255)

                                                                gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtgg                                                 >  1:4481430‑M2/1‑126 (MQ=255)

                                                                gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTGGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtggggg                                           <  2:2295134‑M2/140‑15 (MQ=255)

                                                                gTTGCCTGCGGCAACGCTCACTCGCTGACGCTCGGGCCGAACCTTCGGCGAGGCTTCTTACCTTTCCGCGCATGGGCAGACTATTTGATTGCCGATTCGTTAGAGA‑GTGCAGGCACTTTTGGAAGTGATGGTGGTggggg                                           <  2:4128022/140‑1 (MQ=2)

                                                                     cTGCGGTATCGCTCTCTCGCTGCCGCTCGAGCCGAACGGTAGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGCATATTTGATTGCGGAGTCGCTTGAGG‑GTTCAGGGACTTTTGGAAGTGATGGTGGTGGGGGAAGGa                                      <  2:3210352/140‑1 (MQ=11)

                                                                     cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                                      >  2:5172256‑M2/1‑121 (MQ=255)

                                                                     cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                                      >  1:3475750‑M2/1‑121 (MQ=255)

                                                                     cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                                      >  2:4978079‑M2/1‑121 (MQ=255)

                                                                     cTGCGGCAACGCTCTCTCGCTGACGATCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                                      >  1:2741601‑M2/1‑121 (MQ=255)

                                                                     cTGAGGCAACGCTCTCTCGTTGAGGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATGTTTGATTGGGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                                      >  1:4809815‑M2/1‑121 (MQ=255)

                                                                          gtaacgCTCTTTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattact                                 >  1:911112‑M2/3‑116 (MQ=255)

                                                                          gCAACGCTCTCTCGCTGGCGCTCGAGTCGAACTTTGTGCGAAGGTTATCATCCTGCTCTGCACGGGCAGAATAGTTGGCTGCGGATGCGGTGGGTA‑GGTCAGGGACTTTTGGAAGTGATGGTGGTGGGGGAAGGATTCGa                                 <  2:583964/140‑1 (MQ=2)

                                                                          gCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGATTCTCATCTTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGTACTTTTGGAAGTgatggtggtgggggaaggattact                                 >  2:4879051‑M2/1‑116 (MQ=255)

                                                                           cAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactc                                <  1:4877173‑M2/140‑26 (MQ=255)

                                                                           cAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactc                                <  1:1119850‑M2/140‑26 (MQ=255)

                                                                           cAACCCTCTCTCTCCGACGCTCGCGCCGTACCTTAGTCTAAGCTTCTCATCCTTCACCGCCTGGGCCGAGTATTTGATTGCGGATTCGCTTGGCC‑GTTCAGGGACTTGTGAAAGTGATCGCGGTGGGGGAAGGAttactt                                >  2:5153711/1‑136 (MQ=2)

                                                                               gCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcg                            >  1:1871494‑M2/1‑111 (MQ=255)

                                                                               gCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcg                            >  1:2005642‑M2/1‑111 (MQ=255)

                                                                                  ctctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctg                         >  1:1714616‑M2/1‑108 (MQ=255)

                                                                                   tctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgc                        <  1:2807821‑M2/140‑34 (MQ=255)

                                                                                    ctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcg                       >  1:4520369‑M2/1‑106 (MQ=255)

                                                                                         tGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgctt                  >  1:286340‑M2/1‑101 (MQ=255)

                                                                                         tGACGCTCAAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCCGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgctt                  >  2:833094‑M2/1‑101 (MQ=255)

                                                                                           acgctCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgcttcg                >  1:848220‑M2/2‑99 (MQ=255)

                                                                                           acgcTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGaa                                                            >  2:4037005/2‑96 (MQ=255)

                                                                                             gcTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgcttcgcc              <  1:2947858‑M2/140‑44 (MQ=255)

                                                                                             gcTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgcttcgcc              <  1:4158318‑M2/140‑44 (MQ=255)

                                                                                              cgcGAGTCGAACCTTGGTCGAAGGTTCGCGTCCGTCGGCGAATGGGAAGAATATTTAATTGCGGATTCG‑TAGGGAGGTTCAGGGACTTGTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACgg             <  2:4676102/138‑1 (MQ=2)

                                                                                               tCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgcttcgccct            <  2:1004811‑M2/140‑46 (MQ=255)

                                                                                                 gAGCCGAACCTTGGTCGAAGCTTCTCATCGTTCCCCGCGTGGGCAGAGTATTTGATTGCGGCTTAGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgcttcgcccttc          >  2:1989459‑M2/1‑93 (MQ=255)

                                                                                                   gCCGAACCTTAGTCGGAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattacacagcgcgaagcgcttcgccgctggt        <  2:3601240‑M2/140‑50 (MQ=255)

                                                                                                       aaCCTTAGTCGAAGCTTCTCCTCCTTCCCCGCATGGGCAGAATATTTGATGGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgcttcgcccttcgggtcg    >  2:852219‑M2/1‑87 (MQ=255)

                                                                                                       aaCCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgcttcgcccttcgggtcg    >  1:4148133‑M2/1‑87 (MQ=255)

                                                                                                       aaCCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgcttcgcccttcgggtcg    <  1:4423783‑M2/140‑54 (MQ=255)

                                                                                                         ccTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgcttcgcccttcgggtcgtt  >  1:2390599‑M2/1‑85 (MQ=255)

ACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGA‑GTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAG  >  NZ_CP025268/1293069‑1293313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`