Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 159,854 G→A F20F (TTC→TTT)  gluQRS ← tRNA glutamyl‑Q(34) synthetase GluQRS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268159,8540GA99.5% 1358.4 / NA 361F20F (TTC→TTT) gluQRStRNA glutamyl‑Q(34) synthetase GluQRS
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (152/207);  total (154/207)
Fisher's exact test for biased strand distribution p-value = 1.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 362 total aligned reads displayed.

GTTCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTAGCCAGC  >  NZ_CP025268/159718‑159988
                                                                                                                                        |                                                                                                                                      
  tCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATg                                                                                                                                   >  1:1088060/1‑140 (MQ=255)
  tCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATg                                                                                                                                   >  1:3029310/1‑140 (MQ=255)
  tCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATg                                                                                                                                   >  1:1307966/1‑140 (MQ=255)
   ccAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGa                                                                                                                                  >  1:2190105/1‑140 (MQ=255)
    cAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGaa                                                                                                                                 >  1:1867093/1‑140 (MQ=255)
      gCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGc                                                                                                                               >  2:4554587/1‑140 (MQ=255)
      gCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGc                                                                                                                               >  1:1685630/1‑140 (MQ=255)
      gCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGc                                                                                                                               >  2:3662849/1‑140 (MQ=255)
           cgcAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGcc                                                                                                                          >  2:4900172/1‑140 (MQ=255)
           cgcAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGcc                                                                                                                          <  1:1013491/140‑1 (MQ=255)
           cgcAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGcc                                                                                                                          <  1:3647264/140‑1 (MQ=255)
           cgcAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATAACTTCTATGCGTACCAGCCAGAGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATAAGAGAGCCAAAATGAAGCACGcc                                                                                                                          >  2:4335492/1‑140 (MQ=255)
           cgcAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGAGATCTCCTATGCGTACCAGCCAGCGACCTCGCCGGCCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCCCGcc                                                                                                                          >  2:3938195/1‑140 (MQ=255)
                   aGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGgag                                                                                                                  <  1:1783774/140‑1 (MQ=255)
                   aGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGgag                                                                                                                  <  2:2313780/140‑1 (MQ=255)
                    gTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATACCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGgaga                                                                                                                 <  1:496055/140‑1 (MQ=255)
                          gCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCTAGCGACCTTGCCGGTGGCGAGCTTGCAAATAGCTGCTGAGCGCGGCGATCAGAGAGCCAAAATGAAGATCCCCGGAAGGAGAGGGGGc                                                                                                           >  2:851779/1‑140 (MQ=255)
                          gCGGCACCGGGAACTTCACGAGGCGGGTCGATAGCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAAGGAAGCTCGCCGGAAGGAGAGGGGGc                                                                                                           >  2:2182766/1‑140 (MQ=255)
                          gCGGCACCGGGAACTTCACGAGGCGGGACGATATCTTCTATGCGTACCTGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATATCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAAGAGAgggggg                                                                                                           >  2:3819181/1‑139 (MQ=255)
                             gCACCGGGAACTTCACGAGGCGGGTGGATATCTTCTATGCGTAGCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGaa                                                                                                        <  1:4737926/140‑1 (MQ=255)
                             gCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGaa                                                                                                        <  2:212076/140‑1 (MQ=255)
                              cACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGATCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAg                                                                                                       >  1:2897928/1‑140 (MQ=255)
                               cccGGGAACTTCACGCGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                      <  2:1686313/139‑1 (MQ=255)
                               cacGGGAACTTCACGGGGCGGGTCGATATCTTATCGGCGTCCCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGCAGc                                                                                                      <  2:2239494/138‑1 (MQ=255)
                               acccggAACGTCACGAGGCGGGTCGATATGTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                      <  2:1036631/136‑1 (MQ=255)
                               aCCGGGAACTTCCCGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                      <  2:295056/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                      <  1:4727281/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                      <  1:260399/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                      <  1:2371969/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                      <  1:5196036/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                      <  1:608039/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                      <  2:1475046/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                      <  2:4056820/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                      <  1:1939170/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                      <  1:3207618/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                      <  1:2832095/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                      <  1:2399884/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                      <  1:3679658/140‑1 (MQ=255)
                               aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCCGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                      <  1:447461/140‑1 (MQ=255)
                               aCCGGGAACTTCAAGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                      <  2:4518721/140‑1 (MQ=255)
                               aCCGGGAAATCCACGAGGAGGGTCGATCTCTTCTATGCGTACCAGCCAGCGACCTTGCCGCGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAACTGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                      <  2:3752340/140‑1 (MQ=255)
                                 cGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                    <  2:4278773/140‑1 (MQ=255)
                                 cGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                    <  1:2452767/140‑1 (MQ=255)
                                 cGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                    <  2:2545750/140‑1 (MQ=255)
                                 cGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                    >  2:1604941/1‑140 (MQ=255)
                                   ggcaCTTAACGAGGCGGATCGATATCTTCTATGCGTACCAGCCAGCGCCCTTGCCGGGCGCGCGCCTGCAAATAGCTGCCGAGCGCGGCCAGCAGAGAGCCAAAATGAAGCTCGCCGGAAGGCGAGGGGGCGAAGCGGcc                                                                                                  <  2:1195673/137‑1 (MQ=255)
                                   ggAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGcc                                                                                                  <  1:3863447/140‑1 (MQ=255)
                                   ggAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGcc                                                                                                  <  1:913335/140‑1 (MQ=255)
                                   ggAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAAGAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCGCGCCGGGAGGAGAGTGGGCGAAGCGGcc                                                                                                  >  2:4205780/1‑140 (MQ=255)
                                      aCTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAat                                                                                               <  1:717331/140‑1 (MQ=255)
                                       ctgcACGAGGCGGGGCGATATCTTCTAGGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGCGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAata                                                                                              <  2:2460533/137‑1 (MQ=255)
                                       cTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAata                                                                                              <  2:421804/140‑1 (MQ=255)
                                           aCGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACt                                                                                          <  2:3591611/140‑1 (MQ=255)
                                           aCGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACt                                                                                          <  2:1886462/140‑1 (MQ=255)
                                           aCGAGGCGGGTCGATATCTTCTATGCGTACCAGACAGCGACCGTGCCGGGCGCGAGCCTGCAAATAGATGCCGAGCGCGGCGATCAGACAGCCAAAATGAAGCTCGCCGGAAGGAGCGGGGGCGAAGCGGCCAATATACt                                                                                          <  1:3114886/140‑1 (MQ=255)
                                                  gggTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGCAGGGGCCCATATACtatgtggc                                                                                   >  2:5088899/1‑138 (MQ=255)
                                                    gTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGtgtctg                                                                                 >  1:4309383/1‑140 (MQ=255)
                                                    gTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGtgtctg                                                                                 >  1:3525669/1‑140 (MQ=255)
                                                                  tGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAAc                                                                   >  2:2724251/1‑140 (MQ=255)
                                                                  tGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAAc                                                                   <  1:4076595/140‑1 (MQ=255)
                                                                  tGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAAc                                                                   >  1:1175042/1‑140 (MQ=255)
                                                                   tcGTACCAGCCAGCCACCTTCCCGGGCGCGAGGCTGCATCGAGCTCCCTAGCGCGGCGAGCAGGGAGTCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGTCAATATACTGTGTGTCTGTAATCTCTTTTAACa                                                                  <  2:4824446/139‑1 (MQ=255)
                                                                   gCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACa                                                                  <  2:4783408/140‑1 (MQ=255)
                                                                   gCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACa                                                                  <  1:2001722/140‑1 (MQ=255)
                                                                     gTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaa                                                                >  1:3993512/1‑140 (MQ=255)
                                                                       aCCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaaaa                                                              >  2:2393424/1‑140 (MQ=255)
                                                                       aCCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaaaa                                                              >  1:2958677/1‑140 (MQ=255)
                                                                       aCCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaaaa                                                              >  1:5069276/1‑140 (MQ=255)
                                                                       aCCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaaaa                                                              >  2:115465/1‑140 (MQ=255)
                                                                       aCCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaaaa                                                              >  2:1377403/1‑140 (MQ=255)
                                                                       aCCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaaaa                                                              >  2:1770320/1‑140 (MQ=255)
                                                                         cagccagcGACCTTGCCGGGCGCGAGCCTGCAAATATCTGCCGAGGGCGGCGATCAGAGAGCCAAAATGAAGGTCGCCGGAGGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGGCATCTCTTTGAACaaaaaaa                                                            >  2:3146550/1‑139 (MQ=255)
                                                                            ccagcGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTTAACAAAAAATAAg                                                         >  2:4480688/1‑140 (MQ=255)
                                                                            ccagcGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAg                                                         >  1:2755882/1‑140 (MQ=255)
                                                                            ccagcGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAg                                                         >  2:335284/1‑140 (MQ=255)
                                                                               gcGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCg                                                      <  1:1258649/140‑1 (MQ=255)
                                                                               gcGACCTTGCCGGGCGAGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAAGAAGGCg                                                      <  1:2314928/140‑1 (MQ=255)
                                                                                   ccTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAg                                                  <  1:3291314/140‑1 (MQ=255)
                                                                                   ccTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAg                                                  <  1:1452313/140‑1 (MQ=255)
                                                                                   ccTTGCCGGGCGCGAGACTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAg                                                  <  1:4232567/140‑1 (MQ=255)
                                                                                             cgcgAGCCTGCAAATATCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGTAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTCTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGc                                        <  1:2877477/140‑1 (MQ=255)
                                                                                             cgcgAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGc                                        >  2:773696/1‑140 (MQ=255)
                                                                                              gcgAGGCTGCAAATAGCTGCCGAGCGCGGTTATCAGAGAGACAAAATGAAGCTAGACGGAAGGAGAGGGGGTGAAGCGGCAAATATACTGTGTGTCTGTCATTTCTGTGAACAAAAAATAAGGCGGGAGCATTTCCCGcc                                       <  2:5190151/140‑1 (MQ=255)
                                                                                              gcgAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGcc                                       <  2:3501475/140‑1 (MQ=255)
                                                                                              gcgAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGca                                       <  1:963989/140‑2 (MQ=255)
                                                                                              gcgAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCAAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGcc                                       <  1:4427122/140‑1 (MQ=255)
                                                                                                 aGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCtgt                                    >  1:4516439/1‑140 (MQ=255)
                                                                                                      gCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTaa                               <  2:1934338/140‑1 (MQ=255)
                                                                                                              cTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAATGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATg                       >  2:2048380/1‑140 (MQ=255)
                                                                                                              cTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATg                       >  2:659062/1‑140 (MQ=255)
                                                                                                               tGCGGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCTGAAGGAGAGGTGGTTAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGTATTTCCCGCCTGTGGTAAACGTGATgg                      <  2:4998089/140‑1 (MQ=255)
                                                                                                               tGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATgg                      >  2:3218242/1‑140 (MQ=255)
                                                                                                                 ccGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTaaa                              <  2:389764/130‑1 (MQ=255)
                                                                                                                 ccGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAACCGTGATGGaa                    <  2:2683528/140‑1 (MQ=255)
                                                                                                                 ccGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGaa                    <  1:1719119/140‑1 (MQ=255)
                                                                                                                 ccGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGaa                    <  1:335284/140‑1 (MQ=255)
                                                                                                                 ccGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGCCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAATACAAAATAAGCCGGGAGCATTTCCCGCCTGTGGTAAACGTGATggca                    >  2:341719/1‑138 (MQ=255)
                                                                                                                               ccgagagCCAAACTGAAGCTCGGCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTCGGTGTCTGTCATCTCTTTGAACAAAACATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGTTGTAATTagc      <  2:2814249/138‑1 (MQ=255)
                                                                                                                                   gagCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTagccagc  <  1:4799344/140‑1 (MQ=255)
                                                                                                                                   gagCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTagccagc  >  2:4167092/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                      
GTTCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTAGCCAGC  >  NZ_CP025268/159718‑159988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑