BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,653,860	A→G	73 (TAA→TAG)	CXP41_RS08750 →	protein YdfC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,653,860	0	A	G	99.7%	1042.6 / NA	306	73 (TAA→TAG)	CXP41_RS08750	protein YdfC
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (169/136); minor base T (0/1); total (169/137)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 307 total aligned reads displayed.

CAGCGCAGGATCTGAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGATATTTCCCTGAAAAGTCAGACCATAA  >  NZ_CP025268/1653724‑1653996

  gcgcAGGATCTGAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTACGCATTGCCAGACTGATTACATACCCGCGTTAGTAGCt                                                                                                                                      >  2:3884121/1‑140 (MQ=255)

   cgcAGGATCTGAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTa                                                                                                                                     >  1:3578501/1‑140 (MQ=255)

   cgcAGGATCTGAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTa                                                                                                                                     <  1:2787835/140‑1 (MQ=255)

   cgcAGGATCTGAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCAGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTa                                                                                                                                     <  1:4737902/140‑1 (MQ=255)

    gcAGGATCTGAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTaa                                                                                                                                    >  2:2667909/1‑140 (MQ=255)

     cAGGATCTGAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAAt                                                                                                                                   <  1:1070525/140‑1 (MQ=255)

     cAGGATCTGAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAAt                                                                                                                                   <  1:1823355/140‑1 (MQ=255)

        gATCTGAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATcaa                                                                                                                                <  1:3998593/140‑1 (MQ=255)

        gATCTGAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATcaa                                                                                                                                <  2:2944272/140‑1 (MQ=255)

        gATCTGAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATcaa                                                                                                                                <  2:5118382/140‑1 (MQ=255)

          tCTGAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATcaaca                                                                                                                              <  1:2157170/140‑1 (MQ=255)

          tCTGAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATcaaca                                                                                                                              <  1:4125281/140‑1 (MQ=255)

          tCTGAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATcaaca                                                                                                                              <  1:1743646/140‑1 (MQ=255)

             gAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAg                                                                                                                           >  1:4425428/1‑140 (MQ=255)

             gAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAg                                                                                                                           >  1:3323795/1‑140 (MQ=255)

             gAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAg                                                                                                                           >  2:2513002/1‑140 (MQ=255)

               aaTGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCt                                                                                                                         <  1:2606704/140‑1 (MQ=255)

               aaTGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCt                                                                                                                         <  2:4699681/140‑1 (MQ=255)

               aaTGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCt                                                                                                                         <  2:4284886/140‑1 (MQ=255)

                aTGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTa                                                                                                                        <  1:2518561/140‑1 (MQ=255)

                  gggCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCt                                                                                                                         <  1:3398448/137‑1 (MQ=255)

                  gggCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAg                                                                                                                      >  1:436778/1‑140 (MQ=255)

                  gggCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAg                                                                                                                      >  2:3463353/1‑140 (MQ=255)

                  gggCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAg                                                                                                                      >  2:4053980/1‑140 (MQ=255)

                   ggCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAgg                                                                                                                     >  2:2829122/1‑140 (MQ=255)

                   ggCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAgg                                                                                                                     >  1:1327868/1‑140 (MQ=255)

                     cTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGtt                                                                                                                   <  1:1022440/140‑1 (MQ=255)

                     cTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGtt                                                                                                                   >  1:153693/1‑140 (MQ=255)

                      gaaTGAAGCCAGGGCCAAGCAGGCTGAAAATCTGTCCGCTTTGGAGCAGGATATCACCCATCTGGAAAACGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTGGCTAATCAACAAAGCGAAGGTTa                                                                                                                  >  2:1320718/2‑140 (MQ=255)

                       aaTCAAGCCAGGGCTAAGCAGGCTCAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAg                                                                                                                 <  2:784469/140‑1 (MQ=255)

                          gAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTaa                                                                                                              <  1:4037284/140‑1 (MQ=255)

                              ccAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTaa                                                                                                          >  1:4900935/1‑140 (MQ=255)

                              ccAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTaa                                                                                                          >  2:869607/1‑140 (MQ=255)

                              ccAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTaa                                                                                                          >  1:2078567/1‑140 (MQ=255)

                                aGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGtt                                                                                                                   <  1:1805575/129‑1 (MQ=255)

                                  ggCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAg                                                                                                      >  2:231414/1‑140 (MQ=255)

                                        gCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTcc                                                                                                <  2:3403228/140‑1 (MQ=255)

                                          aGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAc                                                                                              <  1:2932767/140‑1 (MQ=255)

                                          aGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAc                                                                                              <  1:930315/140‑1 (MQ=255)

                                            gCTGAAAATCTGTCCGCTCTGGAGCAGGATATCAGCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGACTGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑gg                                                                                           <  2:4624972/140‑1 (MQ=255)

                                            gCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATcaaca                                                                                                                              >  1:1007355/1‑106 (MQ=255)

                                            gCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCGAC‑gg                                                                                           >  1:5148477/1‑140 (MQ=255)

                                            gCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑gg                                                                                           >  1:4590298/1‑140 (MQ=255)

                                            gCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑gg                                                                                           >  2:2759106/1‑140 (MQ=255)

                                            gCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑gg                                                                                           >  2:1000639/1‑140 (MQ=255)

                                            gCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑gg                                                                                           <  2:237828/140‑1 (MQ=255)

                                            gCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCGCCAC‑gg                                                                                           >  1:3162595/1‑140 (MQ=255)

                                              tGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGt                                                                                         <  2:4806268/140‑1 (MQ=255)

                                              tGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGt                                                                                         <  2:3549667/140‑1 (MQ=255)

                                              tGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGt                                                                                         <  1:855003/140‑1 (MQ=255)

                                               caaaaTCTGTCCGCTCTGGAGCAGGATATCCCCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTg                                                                                        <  2:2406956/139‑1 (MQ=255)

                                                 aaaTCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAAGTAAGGATTTCTCCACTGGGTgg                                                                                       >  1:1344940/1‑139 (MQ=255)

                                                           gCTCTGGAGCAGGATATCACCAATCCGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAgtgcg                                                                            <  2:1766187/140‑1 (MQ=255)

                                                            ctctGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAgtgcgt                                                                           <  2:948934/140‑1 (MQ=255)

                                                            ctctGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAgtgcgt                                                                           >  1:4499795/1‑140 (MQ=255)

                                                               tcgAGCAGGATATCAGACATCTGGAACAAGCAGCGGCATTAAGCATTGCCAGCATGATTACATACCCGCGTTAGTCGCTAATCGACAACCCTACGGTTAGTAATTACGGAGTTCTCCAC‑GGGTGAGGTGGAGtgcgtgcg                                                                        <  2:1181640/138‑1 (MQ=255)

                                                               tGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGtgcgtgcg                                                                        <  2:924372/140‑1 (MQ=255)

                                                                    cAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGa                                                                   <  2:3393343/140‑1 (MQ=255)

                                                                      ggATATCCCCCATCTGGAAAAAGCAGCGGCCTTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTCATCAACAACGCTAAGGTGAGTAATTCAGGCGTTCTCCCC‑GGCTGAGGTGGAGTGCGTGCGCCGGaca                                                                 <  2:1260561/140‑1 (MQ=255)

                                                                       gATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATcaa                                                                                                                                >  2:1564814/1‑77 (MQ=255)

                                                                       gATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGGGAGGTGGAGTGCGTGCGCCGGacac                                                                >  1:4666457/1‑140 (MQ=255)

                                                                                 aTCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGTTTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGAGACGGGTGCGCAt                                                      >  2:4059602/1‑140 (MQ=255)

                                                                                 aTCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATAGCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCAt                                                      >  2:4601557/1‑140 (MQ=255)

                                                                                 aTCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCt                                                                                                                         >  1:2792222/1‑74 (MQ=255)

                                                                                 aTCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCt                                                                                                                         <  2:2792222/74‑1 (MQ=255)

                                                                                 aTCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCAt                                                      >  2:50293/1‑140 (MQ=255)

                                                                                 aTCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCAt                                                      >  2:529068/1‑140 (MQ=255)

                                                                                 aTCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCAt                                                      >  2:3992620/1‑140 (MQ=255)

                                                                                 aTCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCAt                                                      >  2:3782479/1‑140 (MQ=255)

                                                                                 aTCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCAt                                                      >  2:821200/1‑140 (MQ=255)

                                                                                 aTCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCAt                                                      >  1:1141260/1‑140 (MQ=255)

                                                                                 aTCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCAt                                                      >  1:5197608/1‑140 (MQ=255)

                                                                                  tCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATc                                                     >  2:1513309/1‑140 (MQ=255)

                                                                                   cTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATcc                                                    <  2:204180/140‑1 (MQ=255)

                                                                                      gAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAACGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGa                                                 >  1:3919958/1‑140 (MQ=255)

                                                                                          aaGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTg                                             <  1:3060468/140‑1 (MQ=255)

                                                                                            gcagcGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGAc                                           <  2:4151765/140‑1 (MQ=255)

                                                                                             aagcGGCATTAAGCATTGCCAGAACGTGTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTACGGCGTTCTCCAC‑GTGTGAGGTTGAGTGCGTGCGCCCGACACGGGTGCGCATCCGGAACTGACa                                          <  2:1433149/139‑1 (MQ=255)

                                                                                               gcGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCCC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGt                                        <  1:3819496/140‑1 (MQ=255)

                                                                                                cGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGtt                                       <  1:5065064/140‑1 (MQ=255)

                                                                                                cGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGtt                                       >  1:1450809/1‑140 (MQ=255)

                                                                                                cGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGtt                                       >  2:3650330/1‑140 (MQ=255)

                                                                                                cGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGtt                                       >  1:3795983/1‑140 (MQ=255)

                                                                                                             tGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGata                          >  1:4687632/1‑140 (MQ=255)

                                                                                                             tGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGata                          >  1:1604777/1‑140 (MQ=255)

                                                                                                             tGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGata                          >  1:3433415/1‑140 (MQ=255)

                                                                                                             tGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGata                          >  1:560477/1‑140 (MQ=255)

                                                                                                             tGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGGGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGata                          >  2:3437997/1‑140 (MQ=255)

                                                                                                               ccAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGATAtt                        <  1:282073/140‑1 (MQ=255)

                                                                                                                  gAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGATATTTcc                     >  2:1076186/1‑140 (MQ=255)

                                                                                                                   aaTGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGATATTTcgc                    >  2:1894968/1‑138 (MQ=255)

                                                                                                                    aTGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTGCAC‑GGGGGAGGTGGAGTGCGGGCGCCGGACACGGGTGCGCAGCCGGAACTGACAGTTTACTGAAAGGATCTTTCACt                   >  2:255885/1‑140 (MQ=255)

                                                                                                                      gATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGATATTTCCCTGa                 <  1:2271061/140‑1 (MQ=255)

                                                                                                                       aTTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGATATTTCCCTGaa                >  2:2266460/1‑140 (MQ=255)

                                                                                                                         tACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGATATTTCCCTGaaaa              >  2:1582600/1‑140 (MQ=255)

                                                                                                                         tACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGATATTTCCCTGaaaa              >  2:3186615/1‑140 (MQ=255)

                                                                                                                         tACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGGAATTAAGGAGTTCTCCAG‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGTAACTGACAGTTTACTGAAAGGATATTTCCCTGaaaa              >  2:4350293/1‑140 (MQ=255)

                                                                                                                            aTACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGATATTTCCCTGAAAAGTc           <  2:724489/140‑1 (MQ=255)

                                                                                                                                   cgTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGTAACTGACAGTTTACTGAAAGGATATTTCCCTGAAAAGTCAGACCAt    >  1:4079872/1‑140 (MQ=255)

                                                                                                                                   cgTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGATATTTCCCTGAAAAGTCAGACCAt    >  2:666486/1‑140 (MQ=255)

CAGCGCAGGATCTGAAATGGGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAC‑GGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGATATTTCCCTGAAAAGTCAGACCATAA  >  NZ_CP025268/1653724‑1653996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`