BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,660,166	T→C	K109R (AAG→AGG)	ynfA ←	YnfA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,660,166	0	T	C	99.4%	555.4 / NA	172	K109R (AAG→AGG)	ynfA	YnfA family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (71/100); minor base G (0/1); total (71/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 172 total aligned reads displayed.

TAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTG‑C‑G‑C‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCC  >  NZ_CP025268/1660029‑1660299

taAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTa                                                                                                                                           >  2:3497514/1‑140 (MQ=255)

    gATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAACTGGATTTATGTTCCTACGTg                                                                                                                                       <  2:5137157/140‑1 (MQ=255)

     aTCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTg‑c                                                                                                                                     >  1:1044959/1‑140 (MQ=255)

     aTCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTg‑c                                                                                                                                     >  1:3357525/1‑140 (MQ=255)

       cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTg‑c‑g‑c                                                                                                                                 >  1:3729949/1‑140 (MQ=255)

        gAAAAAAGCCGGAGTGATCACAAAAAACGTCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATATAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTAGGCTCCTACGTG‑C‑G‑c‑c                                                                                                                               >  2:2542776/1‑140 (MQ=255)

        gAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑G‑c‑c                                                                                                                               >  1:2291473/1‑140 (MQ=255)

        gAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑G‑c‑c                                                                                                                               >  1:395518/1‑140 (MQ=255)

            aaaGACGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTGTTGTCAGCTGACACTTCCTCTTATCTTATGGATAAAATGGAGTTCTGTTCCTACGTG‑C‑G‑c‑c‑ccca                                                                                                                          <  2:3765930/140‑3 (MQ=255)

               gCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑G‑c‑c‑cccagcc                                                                                                                       <  2:3440635/140‑6 (MQ=255)

                 cGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCg                                                                                                                     <  1:295296/140‑1 (MQ=255)

                 cGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCg                                                                                                                     <  1:4225296/140‑1 (MQ=255)

                    aGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑CAC‑C‑C‑CC‑ATCCCGcaa                                                                                                                  >  2:2832963/1‑140 (MQ=255)

                      tGATCACAAAAAAATGCGTATATTTGCGTTGGGAATGGTTGACAAAAGTTTAAAAAGAATACCTTTTGTCAGCTGACTCTTCCTGTTCTCTTATTGACAAAATGTATTTATTTTGCTACGTG‑C‑GCC‑C‑CC‑ATCCCGcaaca                                                                                                                <  2:3154627/140‑1 (MQ=255)

                      tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGcaaca                                                                                                                <  2:3260465/140‑1 (MQ=255)

                      tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTGTTGTCAGCTGACACGTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGcaaca                                                                                                                <  1:4830124/140‑1 (MQ=255)

                        aTCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAAt                                                                                                              >  1:1679745/1‑140 (MQ=255)

                           acaAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGAt                                                                                                           <  1:4994326/140‑1 (MQ=255)

                                aaaaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACa                                                                                                      >  1:4057960/1‑140 (MQ=255)

                                aaaaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACa                                                                                                      >  2:5176103/1‑140 (MQ=255)

                                      cGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGc                                                                                                <  1:3116593/140‑1 (MQ=255)

                                       gTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCa                                                                                               >  1:573849/1‑140 (MQ=255)

                                       gTATAATTGCGCTCTGAATGTTTGCCAACAGATCAAATAGAATGCCGTTTGTCAGCTGACCCTTCCTCTTATCTTATTGATCAACTGGATTTATGTGCCTACTTGCC‑C‑C‑C‑CC‑AGCCCGCAGCAATGATCAACATTCCGCa                                                                                               <  2:703123/140‑1 (MQ=255)

                                               gcgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCaa                                                                                       >  1:3195675/1‑140 (MQ=255)

                                                cgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAAt                                                                                      <  1:385592/140‑1 (MQ=255)

                                                cgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAAt                                                                                      <  2:2893332/140‑1 (MQ=255)

                                                   tgtgACTGGTTGACAAAAGATGAACTAGCATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTCTGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACACTGATCAACATGCCGCAAAGCGCAATCaa                                                                                   <  2:2424583/140‑1 (MQ=255)

                                                   tgtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCaa                                                                                   <  1:4680582/140‑1 (MQ=255)

                                                    gtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAAc                                                                                  >  2:505394/1‑140 (MQ=255)

                                                     tgtaTGGTTGACAATAGATGTAATAGAATACCTTTTGTCAGCTGACCTTTCCTCTTATCTTATTGATAAAATGTATTTATGTTACTACGTG‑C‑G‑C‑CTCC‑AGCCCGCATCAATGATCAACATGCCTCAAAGCTCAATCAAct                                                                                 >  2:1435943/4‑139 (MQ=255)

                                                     tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                 <  1:594685/140‑1 (MQ=255)

                                                       aaTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCa                                                                               >  2:743074/1‑140 (MQ=255)

                                                       aaTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCa                                                                               <  1:455129/140‑1 (MQ=255)

                                                        aTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                              <  2:1193546/140‑1 (MQ=255)

                                                        aTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                              <  1:2176616/140‑1 (MQ=255)

                                                        aTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                              <  1:2176212/140‑1 (MQ=255)

                                                           gTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACCCTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCg                                                                           <  1:1166594/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGGTAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                         <  2:2709034/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                         <  1:4682796/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                         <  2:4576796/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                         <  1:1416402/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                         <  1:1230887/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                         <  1:371461/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATATTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                         <  2:4629239/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCGCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTC‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                         <  2:5019592/140‑1 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                       >  1:3742890/1‑140 (MQ=255)

                                                                 aaaaGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGt                                                                     >  2:1815505/1‑140 (MQ=255)

                                                                     gATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATa                                                                 <  1:1889176/140‑1 (MQ=255)

                                                                      aTGAAATCGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATaa                                                                >  1:4900171/1‑140 (MQ=255)

                                                                          aaTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAGTGGATTTAGGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCGGCAACAAGGAGCAACATGCCGCAAAGCGCAATCAACGCACCCGGCCAGTCATAAagag                                                            >  2:636617/1‑140 (MQ=255)

                                                                           aTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGAGTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGGGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                           <  2:362951/140‑1 (MQ=255)

                                                                           aTAGAAGACCTTTTGTCAGCTTACACTTCCTCTTATCTTATTTATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                           <  2:2328450/140‑1 (MQ=255)

                                                                                aTACCTTTTGTCAGCTGACACTGCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑G‑C‑C‑CCGAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCGACGCACCCGTCCAGGCATAAAGAGTCAGtt                                                      >  1:1025385/1‑140 (MQ=255)

                                                                                 tACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGttt                                                     >  1:1650363/1‑140 (MQ=255)

                                                                                     ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACCATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                 <  2:4934806/140‑1 (MQ=255)

                                                                                     ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                 <  2:936146/140‑1 (MQ=255)

                                                                                     ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                 <  1:990372/140‑1 (MQ=255)

                                                                                      tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                >  2:4766031/1‑140 (MQ=255)

                                                                                              cTGCCACTTCCTCTTATCTTATTGATAAACTAGATTTATGTTCCTACGTG‑C‑G‑C‑CACC‑AGTCCGCAACAATGATCAACATGGCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAATTTCATGCCATCaaca                                        >  2:2420498/1‑140 (MQ=255)

                                                                                                   acTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgcg                                   <  2:559876/140‑1 (MQ=255)

                                                                                                   acTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgcg                                   <  1:2685587/140‑1 (MQ=255)

                                                                                                       ccgcttatcttatTGATAAAATTGATGTATGTTCCTACGTG‑A‑G‑CTC‑CC‑AGCCCGCAACAGTGTTCATGATTCCGCAAAGCGCAATCGAGGCACCCACTCAGGAAGAAATAGTAAGTTTCAGGTCATCCACAAcgcgttac                               >  2:430444/4‑136 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATGAACATGCCGCAAGGCGCAATAAACGCACCCGTCCAGGCATAACGAGTCAGTTTCACGCGAGCCACAACGCGAAGc                               >  2:432082/1‑140 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                               <  1:2949649/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                               <  1:3203340/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                               <  1:5176103/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                               <  2:4508724/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                               <  1:620866/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                               <  1:829032/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                               <  2:2249378/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTAATAAAATGGATTTATGTCCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCACCGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCAACCACAACGCGCAGc                               <  1:5025911/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTCATTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAGCAATGATCAACATGCCGCAAAGCGCAATCAACGCTCCCGTCCAGTCATAAAGAGTGAGTTTCACGCCATCCACAACGCGCAGc                               <  2:3807140/140‑1 (MQ=255)

                                                                                                             atcttatTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATc                         >  1:4260193/1‑140 (MQ=255)

                                                                                                              tcttacTGATAACATGGATTTATGTCCCAACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCa                        <  2:4192866/140‑1 (MQ=255)

                                                                                                               cttatTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCaa                       <  1:1333520/140‑1 (MQ=255)

                                                                                                                 tatTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                     <  1:4561577/140‑1 (MQ=255)

                                                                                                                 tatTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                     >  1:289918/1‑140 (MQ=255)

                                                                                                                 tatTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                     <  2:1938747/140‑1 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAGAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                    >  1:4416844/1‑140 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                    >  2:616188/1‑140 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                    >  1:1529036/1‑140 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                    >  2:1490998/1‑140 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGGCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACgg                    >  2:1545356/1‑139 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGCG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATTCACAACGCGCAGCCACATAAAAGc                    >  2:2692101/1‑140 (MQ=255)

                                                                                                                   ttGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                   >  1:3594319/1‑140 (MQ=255)

                                                                                                                   ttGATAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                   >  1:4682610/1‑140 (MQ=255)

                                                                                                                      aTAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                <  1:4072614/140‑1 (MQ=255)

                                                                                                                      aTAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                <  2:1422810/140‑1 (MQ=255)

                                                                                                                       tAAAATGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGc               >  2:3088079/1‑140 (MQ=255)

                                                                                                                        aaaaTGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCCAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCa              <  1:4338844/140‑1 (MQ=255)

                                                                                                                        aaaaTGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCa              <  1:3552872/140‑1 (MQ=255)

                                                                                                                        aaaaTGGATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCa              >  1:3482577/1‑140 (MQ=255)

                                                                                                                              gATTTATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATa        <  2:319649/140‑1 (MQ=255)

                                                                                                                                tttATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATaaa      >  1:3050546/1‑140 (MQ=255)

                                                                                                                                  tATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg    <  1:281182/140‑1 (MQ=255)

                                                                                                                                  tATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg    <  1:2830677/140‑1 (MQ=255)

                                                                                                                                  tATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg    <  2:1054118/140‑1 (MQ=255)

                                                                                                                                  tATGTTCCTACGTG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg    <  2:885296/140‑1 (MQ=255)

                                                                                                                                  tATGTTCCTACCTG‑C‑GAC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCCACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg    <  1:4148404/140‑1 (MQ=255)

                                                                                                                                    tGTTCCTACGCG‑C‑GCC‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTAAGTTTCACGCCATCCACAACGCGCAGCCACATCACCGCCGTGCAGACATAAACGcc  >  2:3959/1‑140 (MQ=255)

TAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTG‑C‑G‑C‑C‑CC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCC  >  NZ_CP025268/1660029‑1660299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`