BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,696,010	A→G	Q447Q (CAA→CAG)	ydgA →	YdgA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,696,010	0	A	G	99.2%	872.6 / NA	259	Q447Q (CAA→CAG)	ydgA	YdgA family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (94/163); minor base C (0/2); total (94/165)
Fisher's exact test for biased strand distribution p-value = 5.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 259 total aligned reads displayed.

TGGATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAA  >  NZ_CP025268/1695874‑1696144

 ggATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGt                                                                                                                                    <  1:1235862/140‑1 (MQ=255)

   aTGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTc                                                                                                                                  <  1:3299897/140‑1 (MQ=255)

        aaaCTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCt                                                                                                                             <  1:1034499/140‑1 (MQ=255)

        aaaCTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCt                                                                                                                             <  1:1279407/140‑1 (MQ=255)

          aCTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGa                                                                                                                           >  2:4440820/1‑140 (MQ=255)

             gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                        <  2:4679254/140‑1 (MQ=255)

             gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                        <  1:1536058/140‑1 (MQ=255)

             gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                        <  2:3896253/140‑1 (MQ=255)

             gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                        <  1:1825411/140‑1 (MQ=255)

             gACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGacca                                                                                                                        <  1:2430721/140‑1 (MQ=255)

                  ttCCGGTGGATATGGCAACTGAGTTTATGAGTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGAATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTg                                                                                                                   <  2:4321945/140‑1 (MQ=255)

                  ttCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGACGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTg                                                                                                                   <  2:4448699/140‑1 (MQ=255)

                  ttCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTg                                                                                                                   <  1:610448/140‑1 (MQ=255)

                  ttCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTg                                                                                                                   <  2:731844/140‑1 (MQ=255)

                  ttCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTg                                                                                                                   <  2:1384204/140‑1 (MQ=255)

                  ttCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTg                                                                                                                   <  1:4373429/140‑1 (MQ=255)

                  ttCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTg                                                                                                                   <  1:1111730/140‑1 (MQ=255)

                  ttCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTg                                                                                                                   <  1:1682260/140‑1 (MQ=255)

                  ttCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTCGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTg                                                                                                                   <  1:4430321/140‑1 (MQ=255)

                  ttCCGGTCGATATGGCAACGGGGTTTAGTACTCAGGTAGCGAACCTCGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCCAAACAGCAAGTTGAATGTGCATCAGCAATGGGGCAGATGTTCCGTATGAACACCTTg                                                                                                                   <  2:3738102/140‑1 (MQ=255)

                   tCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGc                                                                                                                  >  2:5056216/1‑140 (MQ=255)

                   tCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGc                                                                                                                  >  2:2088101/1‑140 (MQ=255)

                    ccGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCa                                                                                                                 <  1:187548/140‑1 (MQ=255)

                    ccGGTGGATATGCCAACTCCGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGACGGTGCATCAGCAATGGGGCAGACGTTCCGTCTGACCACCTTGCa                                                                                                                 <  2:3271423/140‑1 (MQ=255)

                     cGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAg                                                                                                                >  1:1737100/1‑140 (MQ=255)

                        tGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGAc                                                                                                             >  1:1482959/1‑140 (MQ=255)

                           atatGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAAt                                                                                                          <  2:1478310/140‑1 (MQ=255)

                            tatGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAAATGGCGAAACAGCAAGTTGAAGGTGCATAAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATa                                                                                                         >  2:1960258/1‑140 (MQ=255)

                              tGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATAcc                                                                                                       <  1:4281934/140‑1 (MQ=255)

                              tGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATAcc                                                                                                       <  2:1611225/140‑1 (MQ=255)

                              tGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATAcc                                                                                                       <  2:2669400/140‑1 (MQ=255)

                               ggCAACTGAGTTTATGACTCAGGTAGCGAAGGTGGAAGGTGATCAGGAAGCTAAAGCGGAAAAAATGGCGAAACAGCAAGTTGAAGGTGCATACGCAATGGGGCAGAAGTTCCGTCTGACTAACTTGCAGGACAATAACa                                                                                                      >  2:86751/1‑140 (MQ=255)

                                gCAACTGAGTTTATGAGTCAGGGAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAACAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCAt                                                                                                     <  1:2870167/140‑1 (MQ=255)

                                gCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCAt                                                                                                     <  2:3983712/140‑1 (MQ=255)

                                    cTGAGTTTATGACTCAGGTAGCGCAGATGGAAGGTTATCAGGAAGATCAAGGGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGACAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcacc                                                                                                 >  1:3106228/1‑140 (MQ=255)

                                    cTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGAc                                                                                                             >  2:1546189/1‑128 (MQ=255)

                                    cTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcacc                                                                                                 >  2:3173386/1‑140 (MQ=255)

                                    cTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcacc                                                                                                 >  1:738025/1‑140 (MQ=255)

                                    cTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGGTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAGGGTGCATCAGCAATGGGGCAGATGGTCCGTCTGACCACCGGGCAGGACAATACCATcacc                                                                                                 >  2:1374860/1‑140 (MQ=255)

                                     tGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcacca                                                                                                <  2:2824954/140‑1 (MQ=255)

                                     tGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcacca                                                                                                <  2:949378/140‑1 (MQ=255)

                                     tGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcacca                                                                                                <  1:3751243/140‑1 (MQ=255)

                                     tGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGATGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcacca                                                                                                <  1:4111612/140‑1 (MQ=255)

                                      gAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcaccac                                                                                               <  1:226672/140‑1 (MQ=255)

                                      gAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcaccac                                                                                               <  1:4941828/140‑1 (MQ=255)

                                      gAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcaccac                                                                                               <  2:480045/140‑1 (MQ=255)

                                      gAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcaccac                                                                                               <  1:162262/140‑1 (MQ=255)

                                      gAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATcaccac                                                                                               >  1:422856/1‑140 (MQ=255)

                                       aGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCaccacc                                                                                              <  1:2965163/140‑1 (MQ=255)

                                          ttATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCCAGCGAAAAAACTGGCGAAACAGCCAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGc                                                                                           <  2:1963989/140‑1 (MQ=255)

                                          ttATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGc                                                                                           <  1:4753843/140‑1 (MQ=255)

                                          ttATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGc                                                                                           <  2:300416/140‑1 (MQ=255)

                                          ttATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGc                                                                                           <  1:1000962/140‑1 (MQ=255)

                                          ttATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGc                                                                                           <  2:4938014/140‑1 (MQ=255)

                                           tATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGcc                                                                                          <  2:3401933/140‑1 (MQ=255)

                                           tATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGcc                                                                                          <  1:4629325/140‑1 (MQ=255)

                                            aTGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCt                                                                                         >  1:1978258/1‑140 (MQ=255)

                                                cTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCaa                                                                                     <  2:4986433/140‑1 (MQ=255)

                                                cTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCaa                                                                                     <  2:768545/140‑1 (MQ=255)

                                                 tCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAat                                                                                    >  1:3313368/1‑140 (MQ=255)

                                                 tCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAat                                                                                    >  1:4855006/1‑140 (MQ=255)

                                                  cAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAata                                                                                   <  1:4469919/140‑1 (MQ=255)

                                                   aGGTAGCGAAGCTGGAAGGTTGTAAGGAAGATCAAGCGAAAAAACTGGCGAAACCGCAAGTTGAAGGTGCATCAGAAATGAAGCAGATGTTCCGTCTGACGAGCTTGCAGGACAATCCCATCACCACCAGCCTGCAatat                                                                                  >  2:2487537/1‑140 (MQ=255)

                                                   aGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAatat                                                                                  >  1:2713127/1‑140 (MQ=255)

                                                   aGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAatat                                                                                  >  1:2583078/1‑140 (MQ=255)

                                                     gTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCt                                                                                                                     <  2:1043661/103‑1 (MQ=255)

                                                      tAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCt                                                                               <  1:2096862/140‑1 (MQ=255)

                                                      tAGAGAAACTGGCAGGTTATCAGGAAGATCAAGCGAAAGGACTGGCGAAACAGCAAGTTGATGGTACATCAGCTATGGGGCAGATGTTCCGTCTACTCACCTTGCAGGACAAAACCTTTACCACAAGCCCGCAATCTGCt                                                                               <  2:3605539/140‑1 (MQ=255)

                                                        gCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTaa                                                                             <  2:4772121/140‑1 (MQ=255)

                                                        gCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGCGCATCAGCAATGGCGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTaa                                                                             <  2:3301296/140‑1 (MQ=255)

                                                         cGAAGCTGGAAGGTTATCAGGAAGATAAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGATAATACCATCACCACCAGCCTGCAAAATGCTAAc                                                                            >  2:2722771/1‑140 (MQ=255)

                                                                ggAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGa                                                                     <  1:3220504/140‑1 (MQ=255)

                                                                        aTCAGGCAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTa                                                             >  1:1331326/1‑140 (MQ=255)

                                                                        aTCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTa                                                             >  1:3336414/1‑140 (MQ=255)

                                                                        aTCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTa                                                             >  1:4045385/1‑140 (MQ=255)

                                                                         tCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATGCCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTaa                                                            <  1:4447017/140‑1 (MQ=255)

                                                                         tCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTaa                                                            >  2:1661917/1‑140 (MQ=255)

                                                                              aataTCAAGCGAAAAAACTGGCCAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCCTAACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATggg                                                       <  2:4092557/137‑1 (MQ=255)

                                                                                gATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCa                                                     >  2:808260/1‑140 (MQ=255)

                                                                                gATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCa                                                     >  1:2360894/1‑140 (MQ=255)

                                                                                gATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCa                                                     >  2:1134264/1‑140 (MQ=255)

                                                                                gATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCa                                                     >  1:3533632/1‑140 (MQ=255)

                                                                                       cGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATg                                              >  1:272896/1‑140 (MQ=255)

                                                                                        gAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGt                                             <  1:5195450/140‑1 (MQ=255)

                                                                                              aCTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTag                                       <  2:1850347/140‑1 (MQ=255)

                                                                                                 ggCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTagaag                                    >  1:4666939/1‑140 (MQ=255)

                                                                                                           cAAGTTGAAGGTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTgg                          <  1:784739/140‑1 (MQ=255)

                                                                                                                    ggTGCATCAGGAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGc                 <  1:3874223/140‑1 (MQ=255)

                                                                                                                    ggTGCATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGc                 <  1:349602/140‑1 (MQ=255)

                                                                                                                        cATCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGTCAGAAATTGTCGTTAGAAGATTTTGTTGGCATTTTTGCGATg             >  1:570416/1‑140 (MQ=255)

                                                                                                                         aTCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTaa                                                                             >  2:1242030/1‑75 (MQ=255)

                                                                                                                         aTCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGc            >  1:2021611/1‑140 (MQ=255)

                                                                                                                         aTCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGc            <  2:2314949/140‑1 (MQ=255)

                                                                                                                          tCAGCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGcc           <  1:1498587/140‑1 (MQ=255)

                                                                                                                          tCAGCAATGGGGCAGATGGTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGcc           <  2:5165980/140‑1 (MQ=255)

                                                                                                                             gCAATGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACAATCACCACCCGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAATATTTTGTTGGCATGTTTGCGATGCCAGc        <  2:3173341/140‑1 (MQ=255)

                                                                                                                                aTGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGc                                                                                           >  2:3835908/1‑54 (MQ=255)

                                                                                                                                 tGGGGCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAATGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTGTGCGATGCCAGCTCtt    >  1:4233668/1‑140 (MQ=255)

                                                                                                                                   gggCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTat  <  2:5040640/140‑2 (MQ=255)

                                                                                                                                   gggCAGATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTaa  >  2:2625735/1‑140 (MQ=255)

TGGATGCGAAACTGACCATTCCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCAAATGTTCCGTCTGACCACCTTGCAGGACAATACCATCACCACCAGCCTGCAATATGCTAACGGTCAGATAACGTTAAATGGGCAGAAAATGTCGTTAGAAGATTTTGTTGGCATGTTTGCGATGCCAGCTCTTAA  >  NZ_CP025268/1695874‑1696144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`