Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,766,333 A→G C92R (TGT→CGT)  sufD ← Fe‑S cluster assembly protein SufD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,766,3330AG99.4% 1172.0 / NA 350C92R (TGT→CGT) sufDFe‑S cluster assembly protein SufD
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (162/185);  total (163/187)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 350 total aligned reads displayed.

CGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTC  >  NZ_CP025268/1766198‑1766468
                                                                                                                                       |                                                                                                                                       
cGCTTTGTGCCAGGCTTTCCGTCAACTGCAGAACCACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCGGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCa                                                                                                                                     <  2:3536492/140‑1 (MQ=255)
cGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCa                                                                                                                                     <  1:4259567/140‑1 (MQ=255)
      gtgCCAGGTCGTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTAAGTGCGGGCACGTAACGCCCATCGCCa                                                                                                                               <  2:3811643/140‑1 (MQ=255)
           aGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCTTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAacac                                                                                                                          <  2:1129536/140‑1 (MQ=255)
           aGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAacaa                                                                                                                          >  2:4043755/1‑139 (MQ=255)
                 tCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCg                                                                                                                    >  1:1659883/1‑140 (MQ=255)
                   cGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCa                                                                                                                  <  1:413887/140‑1 (MQ=255)
                   cGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCa                                                                                                                  <  2:4464609/140‑1 (MQ=255)
                   cGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCa                                                                                                                  <  1:5118936/140‑1 (MQ=255)
                    gTCAAATGCAGACACCCTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGAACGTAACGCCCATCGACAAACACCAGCCGCAc                                                                                                                 <  2:30135/140‑1 (MQ=255)
                    gTCAAAGGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCCCTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCAc                                                                                                                 <  2:3724677/140‑1 (MQ=255)
                     tCAAATGCAGAAACCCTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                <  2:1186041/140‑1 (MQ=255)
                     tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                <  1:628256/140‑1 (MQ=255)
                     tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                <  2:177823/140‑1 (MQ=255)
                     tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                <  1:1504541/140‑1 (MQ=255)
                     tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                <  1:138120/140‑1 (MQ=255)
                     tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                <  1:4667413/140‑1 (MQ=255)
                          tGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATCTCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTc                                                                                                           <  2:1376590/140‑1 (MQ=255)
                           gCAGAAACCCTTCCGCCTGAATAGCGTCGGGTAAACCTTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTTCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACTCCAGCCGCACGGAGTCt                                                                                                          <  2:5025184/140‑1 (MQ=255)
                           gCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCt                                                                                                          <  1:1427291/140‑1 (MQ=255)
                           gCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCt                                                                                                          <  2:2833117/140‑1 (MQ=255)
                           gCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGCTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCt                                                                                                          <  2:4251417/140‑1 (MQ=255)
                           gCACAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGCTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCt                                                                                                          <  2:4406737/140‑1 (MQ=255)
                               aaaCACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACg                                                                                                      >  1:783893/1‑140 (MQ=255)
                                 acacTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGtt                                                                                                    <  2:3303782/140‑1 (MQ=255)
                                 acacTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGtt                                                                                                    <  2:3914813/140‑1 (MQ=255)
                                 acacTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGtt                                                                                                    <  1:1817793/140‑1 (MQ=255)
                                 acacTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGtt                                                                                                    <  1:238293/140‑1 (MQ=255)
                                     ttCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACg                                                                                                >  2:130960/1‑140 (MQ=255)
                                       ccGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCt                                                                                              >  1:3889819/1‑140 (MQ=255)
                                             gAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGATAAGGCa                                                                                        <  1:254006/140‑1 (MQ=255)
                                                tactgTCGGGTAACCCCTGACGGTCGTCTTTTATGCTCACTTCATATCCGCTTCCTTCCGTTCCATCGCTCAGTGCTGGCACGTAACGCCCATCGACAAACACCAGCCGCACCGATTCTAACGTTGACGCTAAGGCATCa                                                                                     <  2:3617931/136‑1 (MQ=255)
                                                  gcggcgGGTAAGCCCGGGCGGGCGGCGTGAATGCTCACTGCATATCCGCGGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTGACGCCCAGCGACAAACACCAGCCGCACGGAGGCGAACGTTAACGCTAAGGCATCACg                                                                                   <  2:541694/140‑1 (MQ=255)
                                                  gCGTCGGGTAAGCCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                   <  1:221223/140‑1 (MQ=255)
                                                  gCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                   <  2:1473781/140‑1 (MQ=255)
                                                  gCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                   <  1:3566329/140‑1 (MQ=255)
                                                  gCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                   <  2:4639313/140‑1 (MQ=255)
                                                  gCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                   <  2:1145125/140‑1 (MQ=255)
                                                  gCGTCGGGTAAACCCGGACGGTCGTCGTTAATGCTCACTTCATAGCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTACCGCCCATCGACAACCACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                   <  2:1881705/140‑1 (MQ=255)
                                                     tCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctg                                                                                >  1:1011555/1‑140 (MQ=255)
                                                     tCGGGTAAACCCTGACGGTCGTCGTTAATGCGCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctg                                                                                >  2:5132594/1‑140 (MQ=255)
                                                       ggggAACCCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGGGCGGGCACGTAACGCCCATCGACAAACAACAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctgct                                                                              <  1:1686837/140‑1 (MQ=255)
                                                        ggTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGctgctg                                                                             >  2:2969886/1‑140 (MQ=255)
                                                         gTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCtgt                                                                            <  1:2053200/140‑1 (MQ=255)
                                                               ccTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGat                                                                      <  2:80765/140‑1 (MQ=255)
                                                               ccTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGat                                                                      <  1:3185407/140‑1 (MQ=255)
                                                                  gACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATAtc                                                                   >  1:3669391/1‑140 (MQ=255)
                                                                   aCGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTGCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCGCGGAGGCTAACGTTAACGCTAAGGCATCAGGCTGCTGTGGGGATAtct                                                                  >  2:4466478/1‑140 (MQ=255)
                                                                    cGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATAtctc                                                                 <  1:1245692/140‑1 (MQ=255)
                                                                    cGGTCGTCGTTAATGCTCAATTCATATCCGCTGCCATCAGTTGCATCGCTCAGTGCGCGCACGTAACGCCCATCGACAAACACCAGCCGCCCGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATAtctc                                                                 <  2:3385033/140‑1 (MQ=255)
                                                                        cgtcgtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGTGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTccc                                                             >  2:156108/1‑140 (MQ=255)
                                                                        cgtcgtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTccc                                                             <  1:156900/140‑1 (MQ=255)
                                                                        cgtcgtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTccc                                                             >  1:1100240/1‑140 (MQ=255)
                                                                          tcgtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGc                                                           >  2:2892462/1‑140 (MQ=255)
                                                                            gtTAATGCTCACTTCAGATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCaa                                                         <  1:1211314/140‑1 (MQ=255)
                                                                                aTGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCAACGACAAACACCAGCCGCACGGAGTCTAACTTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCt                                                     <  1:1060659/140‑1 (MQ=255)
                                                                                 tGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGTTGCTGTGGGGATATCTCTCCCGCAATGCTg                                                    >  1:405198/1‑140 (MQ=255)
                                                                                     cACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaa                                                <  1:758389/140‑1 (MQ=255)
                                                                                     cACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaa                                                >  2:3486075/1‑140 (MQ=255)
                                                                                      aCTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                                               <  2:1057116/140‑1 (MQ=255)
                                                                                        ttCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACt                                             <  1:2953048/140‑1 (MQ=255)
                                                                                        ttCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACt                                             <  1:4484260/140‑1 (MQ=255)
                                                                                                  cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                   >  2:2132195/1‑140 (MQ=255)
                                                                                                  cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                   >  1:2439670/1‑140 (MQ=255)
                                                                                                  cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                   >  1:987147/1‑140 (MQ=255)
                                                                                                  cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                   >  2:2772052/1‑140 (MQ=255)
                                                                                                  cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                   >  1:4915070/1‑140 (MQ=255)
                                                                                                  cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                   >  1:1278422/1‑140 (MQ=255)
                                                                                                  cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGCAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                   >  2:2518643/1‑140 (MQ=255)
                                                                                                  cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGAAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                   >  1:3807121/1‑140 (MQ=255)
                                                                                                   tGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGAAGTCTAACGTTAACGCTAAGGCATCACGCGGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATc                                  <  2:2388870/140‑1 (MQ=255)
                                                                                                     ccTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAg                                >  2:3143867/1‑140 (MQ=255)
                                                                                                     ccTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAg                                >  2:2184359/1‑140 (MQ=255)
                                                                                                      cTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGc                               <  2:280365/140‑1 (MQ=255)
                                                                                                      cTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGc                               <  2:4370120/140‑1 (MQ=255)
                                                                                                               cATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgc                      >  1:3593552/1‑140 (MQ=255)
                                                                                                                 tCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgcgg                    >  2:1429287/1‑140 (MQ=255)
                                                                                                                 tCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgcgg                    >  2:4818263/1‑140 (MQ=255)
                                                                                                                 tCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgcgg                    >  1:1856226/1‑140 (MQ=255)
                                                                                                                   gCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgcggcg                  <  1:3770785/140‑1 (MQ=255)
                                                                                                                    cTCAGTGCGGGCACGTAACGCCCCTCGACAAACATCAGCGGCACGGGGTCTCACGTTAACGCTAAGGCATCACGCTGCTGTGGGGCTATCTCTCCTCCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCTgcggcgg                 >  2:3049133/1‑139 (MQ=255)
                                                                                                                    cTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGt                 >  1:894320/1‑140 (MQ=255)
                                                                                                                    cTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGt                 >  2:399626/1‑140 (MQ=255)
                                                                                                                         tGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCTCAATGCTGACAAACTGGCTATTGATCAGCCCTTCTAGCGGCGTATAtt            >  1:2502546/1‑140 (MQ=255)
                                                                                                                              gCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAg       >  1:4352846/1‑140 (MQ=255)
                                                                                                                              gCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAg       >  2:2731818/1‑140 (MQ=255)
                                                                                                                              gCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAg       >  1:2623575/1‑140 (MQ=255)
                                                                                                                                 cGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGttt    >  1:2911359/1‑140 (MQ=255)
                                                                                                                                 cGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGttt    <  1:3094609/140‑1 (MQ=255)
                                                                                                                                   tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCa                                                                                        >  2:4566601/1‑54 (MQ=255)
                                                                                                                                   tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  >  1:872974/1‑140 (MQ=255)
                                                                                                                                   tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  >  1:1761641/1‑140 (MQ=255)
                                                                                                                                   tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  >  2:4193747/1‑140 (MQ=255)
                                                                                                                                   tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  >  2:3092390/1‑140 (MQ=255)
                                                                                                                                   tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  >  1:3327736/1‑140 (MQ=255)
                                                                                                                                   tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  >  2:2609624/1‑140 (MQ=255)
                                                                                                                                   tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  >  2:2305008/1‑140 (MQ=255)
                                                                                                                                   tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATACTTCCAGTTTTc  >  2:4738762/1‑140 (MQ=255)
                                                                                                                                   tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGCATATTTCCAGTTTTc  >  1:3244459/1‑140 (MQ=255)
                                                                                                                                   tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGCGATAGCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGGGTATTTCCAGTTTTc  >  1:712754/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                       
CGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTC  >  NZ_CP025268/1766198‑1766468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑