BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,804,700	C→T	K39K (AAG→AAA)	rpmI ←	50S ribosomal protein L35

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,804,700	0	C	T	98.7%	774.0 / NA	223	K39K (AAG→AAA)	rpmI	50S ribosomal protein L35
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (135/84); minor base G (3/0); total (139/84)
Fisher's exact test for biased strand distribution p-value = 2.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 224 total aligned reads displayed.

ATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCG  >  NZ_CP025268/1804565‑1804835

   tgtgCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCg                                                                                                                                  <  1:1678166/140‑1 (MQ=255)

       ctctCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCGCGCGATTACCAGGCCCATATGGCGTTTGGCAACAATGGCGTTCGGAGGCAGGTGGCGTTTAGGTTTGGTCGCttt                                                                                                                              >  2:1950392/1‑140 (MQ=255)

       ctctCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCttt                                                                                                                              >  2:770480/1‑140 (MQ=255)

       ctctCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCttt                                                                                                                              >  2:2723366/1‑140 (MQ=255)

       ctctCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCttt                                                                                                                              >  2:3424692/1‑140 (MQ=255)

       ctctCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCttt                                                                                                                              >  2:3903558/1‑140 (MQ=255)

       ctctCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCttt                                                                                                                              >  2:4462622/1‑140 (MQ=255)

       ctctCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCttt                                                                                                                              >  2:4598987/1‑140 (MQ=255)

       ctctCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCttt                                                                                                                              >  1:1235486/1‑140 (MQ=255)

       ctctCCTGTATCTATATTCTAATTAAAAAGTCAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGCTCGCCTCTGGAAACCACGGCGTCCGGACGCAGGTGACGTTTACGTTTGGTCGCttt                                                                                                                              >  2:4894693/1‑140 (MQ=255)

         ctcCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCttttt                                                                                                                            >  1:1437512/1‑140 (MQ=255)

         ctcCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCttttt                                                                                                                            >  2:5045868/1‑140 (MQ=255)

         ctcCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCttttt                                                                                                                            >  1:5107689/1‑140 (MQ=255)

         ctcCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCttttt                                                                                                                            >  1:2710086/1‑140 (MQ=255)

          tcCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCtttttt                                                                                                                           >  1:703255/1‑140 (MQ=255)

           ccTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTg                                                                                                                          >  1:3848268/1‑140 (MQ=255)

            cTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTgg                                                                                                                         >  2:1268649/1‑140 (MQ=255)

               tATCTATATTCTAATTAAAAAGTTAACAACGTTAACGGCTCATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCa                                                                                                                      <  1:1897357/140‑1 (MQ=255)

               tATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCa                                                                                                                      <  2:2125111/140‑1 (MQ=255)

               tATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCa                                                                                                                      >  2:868945/1‑140 (MQ=255)

                  cTATATTCGAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGaa                                                                                                                   <  1:968212/140‑1 (MQ=255)

                    atatTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAAtt                                                                                                                 >  1:4082599/1‑139 (MQ=255)

                    atatTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAAtg                                                                                                                 >  2:1652861/1‑140 (MQ=255)

                    atatTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAAtg                                                                                                                 <  2:785507/140‑1 (MQ=255)

                    atatTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAAtg                                                                                                                 >  1:1736991/1‑140 (MQ=255)

                    atatTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAAtg                                                                                                                 >  1:4287895/1‑140 (MQ=255)

                    atatTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTGTTGGTCAGAAtg                                                                                                                 >  2:3339407/1‑140 (MQ=255)

                    atatTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACAAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGGTTGGTCGCTTTTGTGGTAAGAAtg                                                                                                                 >  2:2136499/1‑140 (MQ=255)

                      atTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGGCGTTTACGTTTGGTCGCTTTTTTGGGCAGAAtgtg                                                                                                               >  1:1145477/1‑140 (MQ=255)

                      atTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAAtgtg                                                                                                               >  1:297261/1‑140 (MQ=255)

                      atTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAAtgtg                                                                                                               >  1:213585/1‑140 (MQ=255)

                      atTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAAtgtg                                                                                                               >  1:3141274/1‑140 (MQ=255)

                        tCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGAc                                                                                                             <  1:2584309/140‑1 (MQ=255)

                        tCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGGACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGAc                                                                                                             <  2:2511563/140‑1 (MQ=255)

                         cTAATTAAAAAGTTCAAAACGTTAACGGCTTATGGGTACGGCGGGCACGCGATTAGCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGGCGCAGGTGACGTTTACGGTCGGGGGCTGGTTGGGTCAGAATGTGACg                                                                                                            >  2:1762856/1‑140 (MQ=255)

                         cTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACg                                                                                                            >  1:3892148/1‑140 (MQ=255)

                         cTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACg                                                                                                            >  2:3887404/1‑140 (MQ=255)

                         cTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACg                                                                                                            >  2:3886226/1‑140 (MQ=255)

                            aTTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAACCCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAg                                                                                                         >  1:1290366/1‑140 (MQ=255)

                            aTTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAg                                                                                                         >  1:4490688/1‑140 (MQ=255)

                            aTTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAg                                                                                                         >  1:4945973/1‑140 (MQ=255)

                                        aaaaCGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCgtgctt                                                                                             <  2:2529931/140‑1 (MQ=255)

                                         aaaCGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCgtgcttg                                                                                            <  1:4196191/140‑1 (MQ=255)

                                               tAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTa                                                                                      >  1:2169736/1‑140 (MQ=255)

                                               tAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTa                                                                                      >  1:3288933/1‑140 (MQ=255)

                                                 aCGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTaaa                                                                                    <  2:4936153/140‑1 (MQ=255)

                                                     cTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGGTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAacca                                                                                >  1:615023/1‑140 (MQ=255)

                                                       tATGCGTACGGCAGGCACGCGATTACCAGGCCAAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAaccacc                                                                              >  1:240857/1‑140 (MQ=255)

                                                             tACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTAcc                                                                        >  2:5136498/1‑140 (MQ=255)

                                                               cGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCATGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCgg                                                                      >  2:1862107/1‑140 (MQ=255)

                                                               cGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTCCCgg                                                                      >  1:4803123/1‑140 (MQ=255)

                                                               cGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCgg                                                                      >  2:3647029/1‑140 (MQ=255)

                                                               cGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCgg                                                                      >  2:537849/1‑140 (MQ=255)

                                                               cGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCgg                                                                      >  1:4823304/1‑140 (MQ=255)

                                                               cGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATATGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCgg                                                                      >  1:4748381/1‑140 (MQ=255)

                                                                     gcaCGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGtttttt                                                                >  1:1007160/1‑140 (MQ=255)

                                                                       aCGCGATTACCAGGCCAAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTg                                                              <  2:240857/140‑1 (MQ=255)

                                                                          cgATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAg                                                           >  2:2807807/1‑140 (MQ=255)

                                                                          cgATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAg                                                           <  1:1610044/140‑1 (MQ=255)

                                                                          cgATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAg                                                           <  2:2299129/140‑1 (MQ=255)

                                                                          cgATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTATGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAATCCACCTTTACCGGTTTTTTTGAAg                                                           <  2:1166823/140‑1 (MQ=255)

                                                                          cgATTAACAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAg                                                           <  2:1575359/140‑1 (MQ=255)

                                                                            cttACCAGCTCCAGCTCGCACTTGGACACCATGGCTTTCGGACGCAGGTGCCGTTTCCGTCTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                                         <  2:4204724/139‑1 (MQ=255)

                                                                            aTTACCAGGCCCAGATCGGCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAACCCACCTTTCCCGGTTTTTTTGAAgcg                                                         <  1:1711324/140‑1 (MQ=255)

                                                                            aTTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                                         <  2:1304464/140‑1 (MQ=255)

                                                                            aTTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                                         <  2:2328784/140‑1 (MQ=255)

                                                                            aTTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                                         <  1:1773186/140‑1 (MQ=255)

                                                                            aTTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                                         <  1:537849/140‑1 (MQ=255)

                                                                            aTTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGATTTTTTGAAgcg                                                         <  2:5130560/140‑1 (MQ=255)

                                                                            aTTACAAGACCCAGATCACCTTTGGAAACCATGGCTTTCGGACGCCGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGCATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACAGGTTTTTTTGAAgcg                                                         <  2:3071547/140‑1 (MQ=255)

                                                                                   ggCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTagca                                                  >  1:303109/1‑140 (MQ=255)

                                                                                     cccAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCg                                                                                                                                  >  2:256632/1‑58 (MQ=255)

                                                                                     cccAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCg                                                                                                                                  <  1:256469/58‑1 (MQ=255)

                                                                                     cccAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                                         <  1:1536479/131‑1 (MQ=255)

                                                                                     cccAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                                         >  2:1536479/1‑131 (MQ=255)

                                                                                     cccAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTagcagc                                                >  2:577529/1‑140 (MQ=255)

                                                                                     cccAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTAGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTagcagc                                                <  1:3708421/140‑1 (MQ=255)

                                                                                       cAGATCGCCTTTGGAAAGCATGGCTTTCGGACGCAGGTGACGTCTACGTTTGGTCGCGTCTCTGGTCAGAATGGGACGCTGGTTAGGGTGCTTGTGCTTAGAACCACCCTTAGCGGTTTTTTTGAAGCGCTTAGCAGCAc                                              >  2:5099111/1‑140 (MQ=255)

                                                                                       cAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCAc                                              >  1:4924086/1‑140 (MQ=255)

                                                                                        aGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCAcc                                             >  1:4772915/1‑140 (MQ=255)

                                                                                             gCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTa                                        <  1:938698/140‑1 (MQ=255)

                                                                                             gCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTa                                        <  2:703255/140‑1 (MQ=255)

                                                                                             gCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTa                                        <  2:2346420/140‑1 (MQ=255)

                                                                                             gCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTa                                        <  2:1443894/140‑1 (MQ=255)

                                                                                              ccTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCATCACCGCGTAc                                       >  2:3529988/1‑140 (MQ=255)

                                                                                              ccTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGACGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTAc                                       <  2:2922557/140‑1 (MQ=255)

                                                                                                  tGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTc                                   >  2:4386436/1‑140 (MQ=255)

                                                                                                        ccATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAAtt                             >  1:1312675/1‑140 (MQ=255)

                                                                                                         cATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAAttt                            >  2:3599212/1‑140 (MQ=255)

                                                                                                         cATGGCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTAAAAACCACCTTTACCGGTTTTTGTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAAttt                            >  1:2894497/1‑140 (MQ=255)

                                                                                                             gCTTTCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTgg                        <  2:1042166/140‑1 (MQ=255)

                                                                                                                 tCGGACGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCAtt                    >  1:4691955/1‑140 (MQ=255)

                                                                                                                      cGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTtaat               <  2:4660980/140‑1 (MQ=255)

                                                                                                                      cGCAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTtaat               <  2:2169736/140‑1 (MQ=255)

                                                                                                                        cAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                                         >  2:3023108/1‑96 (MQ=255)

                                                                                                                        cAGGTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                                         <  1:3023108/96‑1 (MQ=255)

                                                                                                                           gTGACGTTTACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACtt          <  1:1460250/140‑1 (MQ=255)

                                                                                                                                  ttACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTc   <  1:3886226/140‑1 (MQ=255)

                                                                                                                                   tACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCg  >  1:3411505/1‑140 (MQ=255)

                                                                                                                                   tACGTTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCGTAAAACCACCTTTACCGGTTTTTTTGAAgcg                                                         <  2:1806564/85‑1 (MQ=255)

ATATGTGCTCTCCTGTATCTATATTCTAATTAAAAAGTTAAAAACGTTAACGGCTTATGCGTACGGCAGGCACGCGATTACCAGGCCCAGATCGCCTTTGGAAACCATGGCTTTCGGACGCAGGTGACGTTTACGCTTGGTCGCTTTTTTGGTCAGAATGTGACGCAGGTTAGCGTGCTTGTGCTTAAAACCACCTTTACCGGTTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCG  >  NZ_CP025268/1804565‑1804835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`