BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,930,910	A→G	219 (TAA→TAG)	yobB →	carbon‑nitrogen hydrolase family protein YobB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,930,910	0	A	G	99.5%	1200.3 / NA	364	219 (TAA→TAG)	yobB	carbon‑nitrogen hydrolase family protein YobB
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (232/128); minor base T (1/1); total (234/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 364 total aligned reads displayed.

GGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCG  >  NZ_CP025268/1930775‑1931046

  cAAATGCCCGTGGTACTAGTGCACTATGGGATGAATCTGGTCGTCTCATCGTTCGCGCCGATCGTTGTTCATTATTGTTAGTCGGTCAGCGTTCAGCACAGGGTTGGCAAGGCGCTATCATTCCATTACGCTAGGCtttt                                                                                                                                    <  2:1119935/140‑1 (MQ=255)

     aTGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTTGGCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTATGCTTTTTCg                                                                                                                                 >  1:2543622/1‑140 (MQ=255)

     aTGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTACg                                                                                                                                 >  1:3997665/1‑140 (MQ=255)

       gCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCCCAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGc                                                                                                                               <  2:2166039/140‑1 (MQ=255)

        cccGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATGGTTCGCGCCGATCGCGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGcc                                                                                                                              >  2:4757224/1‑140 (MQ=255)

        cccGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGcc                                                                                                                              <  1:4141950/140‑1 (MQ=255)

        cccGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGcc                                                                                                                              >  1:4502576/1‑140 (MQ=255)

            tGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGa                                                                                                                          >  1:5107994/1‑140 (MQ=255)

              gTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGc                                                                                                                        >  2:2208792/1‑140 (MQ=255)

                 aCAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATg                                                                                                                     >  1:1566320/1‑140 (MQ=255)

                 aCAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATg                                                                                                                     <  2:4502576/140‑1 (MQ=255)

                     tGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGc                                                                                                                               >  2:3802171/1‑126 (MQ=255)

                     tGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGa                                                                                                                          <  2:2609717/131‑1 (MQ=255)

                     tGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGGCAt                                                                                                                 >  2:2325376/1‑140 (MQ=255)

                     tGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCAt                                                                                                                 >  1:828637/1‑140 (MQ=255)

                      gCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATg                                                                                                                <  2:2007058/140‑1 (MQ=255)

                      gCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATg                                                                                                                <  1:1111552/140‑1 (MQ=255)

                      gCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATg                                                                                                                <  1:2303720/140‑1 (MQ=255)

                       cACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGt                                                                                                               >  1:3455558/1‑140 (MQ=255)

                       cACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGt                                                                                                               >  1:4424807/1‑140 (MQ=255)

                       cACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGt                                                                                                               >  1:105401/1‑140 (MQ=255)

                       cACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGt                                                                                                               >  1:5023903/1‑140 (MQ=255)

                        aCTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGtt                                                                                                              <  2:3050070/140‑1 (MQ=255)

                            tGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTgcgc                                                                                                          <  2:5077746/140‑1 (MQ=255)

                            tGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTgcgc                                                                                                          >  2:1185655/1‑140 (MQ=255)

                            tGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTgcgc                                                                                                          >  1:5159938/1‑140 (MQ=255)

                               gATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCAtt                                                                                                       >  1:2622901/1‑140 (MQ=255)

                               gATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCAtt                                                                                                       >  2:1752927/1‑140 (MQ=255)

                                 tGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                     >  1:3048461/1‑140 (MQ=255)

                                 tGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                     >  2:4929034/1‑140 (MQ=255)

                                 tGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                     <  2:2847051/140‑1 (MQ=255)

                                 tGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                     >  1:1743596/1‑140 (MQ=255)

                                 tGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTACTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTAt                                                                                                     <  1:4742032/140‑1 (MQ=255)

                                   aaTATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCg                                                                                                   <  1:774339/140‑1 (MQ=255)

                                    atatGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGa                                                                                                  >  1:2734338/1‑140 (MQ=255)

                                    atatGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGa                                                                                                  >  1:3259361/1‑140 (MQ=255)

                                    atatGGTCGTCTCATCGTTCGCGCCGATCGTGGTGCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTGTTTCGGCCTGGGGCATGCCATGTTGCGCATTATCGa                                                                                                  >  1:684488/1‑140 (MQ=255)

                                         gtcgtcTCATCGTTCGCGCCGAGCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAg                                                                                             >  2:5027105/1‑140 (MQ=255)

                                          tcgtcTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGa                                                                                            <  2:2723312/140‑1 (MQ=255)

                                          tcgtcTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCGGGAGCATGCCATGTTGCGCATTATCGATACAGa                                                                                            >  2:3247867/1‑140 (MQ=255)

                                             tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTGCTTTACGCTAGGCATTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAAc                                                                                         >  1:4309789/1‑140 (MQ=255)

                                             tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAAc                                                                                         >  2:1864943/1‑140 (MQ=255)

                                             tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAAc                                                                                         >  1:911276/1‑140 (MQ=255)

                                             tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAAc                                                                                         >  2:1429722/1‑140 (MQ=255)

                                             tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAAc                                                                                         >  1:29984/1‑140 (MQ=255)

                                             tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAAc                                                                                         >  1:2678507/1‑140 (MQ=255)

                                             tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAAc                                                                                         >  1:3753900/1‑140 (MQ=255)

                                             tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAAc                                                                                         >  1:4273024/1‑140 (MQ=255)

                                             tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAAc                                                                                         >  1:1472209/1‑140 (MQ=255)

                                             tctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAAc                                                                                         >  2:4625829/1‑140 (MQ=255)

                                              ctcATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAATGCGAGATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGaaatc                                                                                        >  2:1552974/1‑138 (MQ=255)

                                                  tCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCg                                                                                    <  1:4028061/140‑1 (MQ=255)

                                                  tCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCg                                                                                    >  1:3852655/1‑140 (MQ=255)

                                                       cgcgCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTg                                                                               <  1:4819646/140‑1 (MQ=255)

                                                       cgcgCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTg                                                                               <  1:2179329/140‑1 (MQ=255)

                                                         cgccgcTCGTGGTTCTTCATTGTCAGTCGGTCAGCGTTCATCACAGCGTTAGCAAGCCGATATCATTCCATTACGCTAGGCTTTCTCGGCCTGGAGCATACCATGTTGCGGATTATCCCTACAGACACCTGCGGTTTGCa                                                                             <  2:4849158/140‑1 (MQ=255)

                                                         cgcCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCa                                                                             >  1:2841905/1‑140 (MQ=255)

                                                          gcCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCag                                                                            <  1:1313853/140‑1 (MQ=255)

                                                            cGATCGTGCTTCATTATTGTTAGTCGGCCAGCGTTCATCACCGGGTTGGCAAGACGACATCATTCCATTACGCTATGCTTTTTCGGCCTGGAGCCTGCCATGTTGCGCCTTATCGATACAGAAACCTGCGGTTTGCaggg                                                                          <  2:4171482/140‑1 (MQ=255)

                                                              aTCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCagggag                                                                        >  1:4981374/1‑140 (MQ=255)

                                                              aTCGTGGTTCATTAGTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCagggag                                                                        <  1:1244233/140‑1 (MQ=255)

                                                                    gTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCg                                                                  >  2:2651093/1‑140 (MQ=255)

                                                                        attattGTTAGTCGGTCAGCGTCCATCACAGGGTTGGCAAGGCGATACCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACCGAAACCTGCAGTTTGCAGGGCGGGATCGTTga                                                              >  2:2866542/1‑140 (MQ=255)

                                                                          gattGTTAGTCGGTGAGCGTTCATCAGAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCGTGGAGCATGCCATTTTGCGCAATATCGATTCAGAAACCTGCGGTTTGTAGGGTGGGATCGTTgcga                                                            >  2:242002/2‑140 (MQ=255)

                                                                           attGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGAt                                                           <  2:39562/140‑1 (MQ=255)

                                                                           attGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGAt                                                           <  2:3549547/140‑1 (MQ=255)

                                                                               ttAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGcc                                                       <  2:4038888/140‑1 (MQ=255)

                                                                                tAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct                                                      <  2:2473122/140‑1 (MQ=255)

                                                                                tAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTGGCCCATTATCGATACCGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCct                                                      <  1:2131786/140‑1 (MQ=255)

                                                                                 aGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCctc                                                     >  1:208135/1‑140 (MQ=255)

                                                                                    cGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGt                                                  >  1:1264832/1‑140 (MQ=255)

                                                                                      gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTTCCTCTGTTg                                                >  2:4141367/1‑140 (MQ=255)

                                                                                      gTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTg                                                >  1:2848587/1‑140 (MQ=255)

                                                                                             ttCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCAt                                         >  1:1955944/1‑140 (MQ=255)

                                                                                                 tcaCAGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGAc                                     >  1:2383470/1‑140 (MQ=255)

                                                                                                     aGGGTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGaa                                 >  2:1662729/1‑140 (MQ=255)

                                                                                                       ggTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCCTGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGaaaa                               >  2:3407346/1‑140 (MQ=255)

                                                                                                       ggTTGGCAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGaaaa                               >  1:3639311/1‑140 (MQ=255)

                                                                                                             cAAGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTc                         <  1:984824/140‑1 (MQ=255)

                                                                                                              aaGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCa                        <  1:4145513/140‑1 (MQ=255)

                                                                                                              aaGGCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCa                        <  1:1429722/140‑1 (MQ=255)

                                                                                                                 gCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAcc                     >  1:4338899/1‑140 (MQ=255)

                                                                                                                 gCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAcc                     >  2:380961/1‑140 (MQ=255)

                                                                                                                 gCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAcc                     >  2:3710635/1‑140 (MQ=255)

                                                                                                                 gCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAcc                     >  2:598906/1‑140 (MQ=255)

                                                                                                                 gCGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAcc                     >  1:239837/1‑140 (MQ=255)

                                                                                                                  cGATATCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAAccc                    <  1:2708749/140‑1 (MQ=255)

                                                                                                                     tatCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCAt                 <  2:3852295/140‑1 (MQ=255)

                                                                                                                       tCATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAAGCGTCAACCCCATGa               <  2:1291520/140‑1 (MQ=255)

                                                                                                                        cATTCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAg              <  1:409723/140‑1 (MQ=255)

                                                                                                                          ttCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGcc            >  1:1845542/1‑140 (MQ=255)

                                                                                                                          ttCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGcc            >  1:2831113/1‑140 (MQ=255)

                                                                                                                           tCCATTACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCa           >  2:825176/1‑140 (MQ=255)

                                                                                                                               ttACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTg       >  2:4378713/1‑140 (MQ=255)

                                                                                                                               ttACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTg       >  2:2911662/1‑140 (MQ=255)

                                                                                                                               ttACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTg       >  2:4990228/1‑140 (MQ=255)

                                                                                                                               ttACGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTg       >  2:2490589/1‑140 (MQ=255)

                                                                                                                                 aCGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGt     >  2:5194222/1‑140 (MQ=255)

                                                                                                                                 aCGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTGTGAAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGt     <  1:3795770/140‑1 (MQ=255)

                                                                                                                                  cGCTAGGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTg    >  2:4705714/1‑140 (MQ=255)

GGCAAATGCCCGTGGTAACAGTGCACTATGGGATGAATATGGTCGTCTCATCGTTCGCGCCGATCGTGGTTCATTATTGTTAGTCGGTCAGCGTTCATCACAGGGTTGGCAAGGCGATATCATTCCATTACGCTAAGCTTTTTCGGCCTGGAGCATGCCATGTTGCGCATTATCGATACAGAAACCTGCGGTTTGCAGGGAGGGATCGTTGAGATTGCCTCTGTTGATGTCATTGACGGAAAAATCGTCAACCCCATGAGCCACCTGGTGCG  >  NZ_CP025268/1930775‑1931046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`