BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,957,477	A→G	132 (TAA→TAG)	yecN →	MAPEG family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,957,477	0	A	G	99.6%	806.7 / NA	246	132 (TAA→TAG)	yecN	MAPEG family protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (130/115); total (131/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

ATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATG  >  NZ_CP025268/1957340‑1957610

gtGCATTATTACGGCTTTCCTCCCCGTCTGTCCCGCTGGCGACGTTCCGGCATGCGCGCCACCTGGTGTGCGCTGTTCCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCCCCCTGCGTTAGCg                                                                                                                                     <  2:1943871/139‑1 (MQ=255)

aTGCATTATTACGATTTTCATCACCGTCTGTTCCGCTGGCGACGTACCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGACGCTGGCGAATCTTTGGTGTATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCg                                                                                                                                     <  2:4495118/140‑1 (MQ=255)

  gCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGca                                                                                                                                   <  1:487094/140‑1 (MQ=255)

       attaCGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATa                                                                                                                              >  2:3642317/1‑140 (MQ=255)

       attaCGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATa                                                                                                                              >  2:3590995/1‑140 (MQ=255)

         taCGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATCCg                                                                                                                            <  2:2185990/140‑1 (MQ=255)

          aCGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGc                                                                                                                           >  2:333631/1‑140 (MQ=255)

           cGGTTTTCATCACCGTATGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCTCTGTTGCTGATGGTGCTGGCGAATCTTTGGCATAGGGCCTGAGAGTTGGTTTTCTCCCTGCGTTAGCGCACACTACGcc                                                                                                                          >  2:2556435/1‑140 (MQ=255)

             gTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCAc                                                                                                                        <  1:4968196/140‑1 (MQ=255)

              ttttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCAct                                                                                                                       <  1:4046194/140‑1 (MQ=255)

                ttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCGActtt                                                                                                                     >  2:2809876/1‑140 (MQ=255)

                ttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCActtt                                                                                                                     >  1:1248385/1‑140 (MQ=255)

                ttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCActtt                                                                                                                     >  1:1248272/1‑140 (MQ=255)

                ttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCActtt                                                                                                                     >  2:1676984/1‑140 (MQ=255)

                ttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCActtt                                                                                                                     >  2:1471509/1‑140 (MQ=255)

                ttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGGTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGGGTTGTTTTTCTCCCTGCTTTAGCGAACAATACGCGAcctt                                                                                                                     >  2:884288/1‑140 (MQ=255)

                ttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCAACTGGTGTGCGCTGTTGCTGATGGTGCTGGCGGATCTTTGGTATATGCCTTGGGAGTTGGGTTTGTCCCTGCGTTAGCGCACAATACGCCActtt                                                                                                                     >  2:4830276/1‑140 (MQ=255)

                ttCATCACCGTCTGTTCCGCTGGCGACATTCCGGCAAGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTCAGTATATGCCCTGGGAGTTGGCTTTCTCCCTGCGATAGCGCACAATAGTCCActtt                                                                                                                     >  2:3804761/1‑140 (MQ=255)

                        cGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTcc                                                                                                             >  2:1211590/1‑140 (MQ=255)

                        cGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTcc                                                                                                             <  1:1795789/140‑1 (MQ=255)

                         gTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTccc                                                                                                            <  1:1613783/140‑1 (MQ=255)

                          tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGTTGGTGCTGGCGAATCTTTGGTATATGCCCTGGTAGTTGGTTTTCTCCCTGCGTTAGCGCCCAATACGCTACTGTCTTTTTCCCg                                                                                                           >  2:31836/1‑140 (MQ=255)

                          tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCg                                                                                                           >  1:2624591/1‑140 (MQ=255)

                          tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCg                                                                                                           >  1:904835/1‑140 (MQ=255)

                          tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCg                                                                                                           >  1:3658071/1‑140 (MQ=255)

                                  gCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTa                                                                                                   <  1:1020681/140‑1 (MQ=255)

                                  gCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTa                                                                                                   <  1:5067823/140‑1 (MQ=255)

                                    tGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACg                                                                                                 <  1:1471509/140‑1 (MQ=255)

                                        gACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTAt                                                                                             >  1:3105492/1‑140 (MQ=255)

                                        gACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTGTCCCGGATTTTTACGTTAt                                                                                             >  2:2896181/1‑140 (MQ=255)

                                            ttCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGtct                                                                                         >  1:1496803/1‑140 (MQ=255)

                                                    tGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGa                                                                                 <  1:4391942/140‑1 (MQ=255)

                                                     gAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGac                                                                                <  1:2137017/140‑1 (MQ=255)

                                                        cgcCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACg                                                                             <  1:2510829/140‑1 (MQ=255)

                                                           cACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTa                                                                          <  2:2659100/140‑1 (MQ=255)

                                                            aCCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCGAt                                                                         >  2:3153203/1‑140 (MQ=255)

                                                                ggTGTGCGCTGTTGCTGTTGGTGCTGGCGCATCTTTGGTATATGCCCTGGGGGTTGGGGTTCTGCCTGCGTTAGCGCACAATACGCCAATTTCTTTTTCCCCGAAATTTACGTTATGTATCACCGCGACACGCTAttttt                                                                     >  2:841428/1‑139 (MQ=255)

                                                                  tgtgCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTCGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTCTTTTCTg                                                                   >  1:3811106/1‑140 (MQ=255)

                                                                     gcgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGccc                                                                >  2:3397823/1‑140 (MQ=255)

                                                                     gcgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGccc                                                                >  2:3397750/1‑140 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGCGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                               <  2:2847496/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                               <  1:626332/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                               <  2:1539413/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                               <  2:1242414/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                               <  1:2750783/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                               >  1:2899256/1‑140 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                               <  2:4202994/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                               >  2:3419397/1‑140 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                               <  1:1591626/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                               <  1:1760666/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                               >  1:4046040/1‑140 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAAGCCTTGGTATAGGCCCTGGGAGTTGGTTTTCTCCCTGCGTGCGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTCCGTTATGTCTCTCCGCGACACGCTATTTTCTGcccc                                                               <  2:670887/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGAGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTGCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                               <  1:4476659/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGACGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                               <  1:238473/140‑1 (MQ=255)

                                                                       gcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCt                                                              >  1:1923683/1‑140 (MQ=255)

                                                                                gATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGa                                                     <  2:3019881/140‑1 (MQ=255)

                                                                                gATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGa                                                     >  2:2101788/1‑140 (MQ=255)

                                                                                gATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGa                                                     >  1:480914/1‑140 (MQ=255)

                                                                                gATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGa                                                     >  1:86881/1‑140 (MQ=255)

                                                                                   ggTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTg                                                  >  1:327937/1‑140 (MQ=255)

                                                                                   ggTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTGTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCTACACGCTATTTTCTGCCCCTTTCGCCAGACTg                                                  >  1:3196719/1‑140 (MQ=255)

                                                                                     tGCTGTCGAATCGTTGGTATGTGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACGTTCTGTTGCGCGGAATTTTACGTTATGTTTCACTGCAACACGCTATTTTGTCCCCCTATCGCAAGCCTggg                                                >  2:2047505/1‑140 (MQ=255)

                                                                                     tGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTggg                                                >  1:3433140/1‑140 (MQ=255)

                                                                                        tGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGa                                             >  1:2216520/1‑140 (MQ=255)

                                                                                           cGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTg                                          <  2:1263131/140‑1 (MQ=255)

                                                                                               tCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACg                                                                                                                            <  2:3674038/54‑1 (MQ=255)

                                                                                               tCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACg                                                                                                                            >  1:3674038/1‑54 (MQ=255)

                                                                                               tCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGAcc                                      >  2:4605817/1‑140 (MQ=255)

                                                                                                 tttGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTAGCGCCAGACTGGGCGACTGGACCtt                                    <  1:2415983/140‑1 (MQ=255)

                                                                                                         atGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAAc                            >  2:4971/1‑140 (MQ=255)

                                                                                                         atGCCCTGGGAGTTGGTGTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAAc                            >  1:1937926/1‑140 (MQ=255)

                                                                                                          tGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACg                           >  1:4294080/1‑140 (MQ=255)

                                                                                                             ccTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGt                        >  2:5020735/1‑140 (MQ=255)

                                                                                                               tGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCAACTTTCTTTTTCGCGGATTTTTACGGTATGTCTCACCCGGACACGCTATTTTCTGCCGCTATCGCCAGAATGGGCGACTGGACAGTTGATGAACGGGTAg                      >  2:2569384/1‑140 (MQ=255)

                                                                                                                gggCGTTGGTTTTCTCCCTGCGTTAGAGCACAATACGCCACTTTCTTTTTCGCGTATTTTTACGTTATGTTTCACCGCGCCACGCTATGTTCTTCCCCTATTGCCAGACTGGGCGCCTGGACCTTGTATGAACGGGTAGc                     >  2:1030351/1‑140 (MQ=255)

                                                                                                                gggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGc                     >  1:1087239/1‑140 (MQ=255)

                                                                                                                gggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGc                     >  2:2235534/1‑140 (MQ=255)

                                                                                                                gggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTATTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGGAGc                     >  2:3857378/1‑140 (MQ=255)

                                                                                                                    gTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGaa                 <  2:856218/140‑1 (MQ=255)

                                                                                                                    gTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGaa                 <  2:4029244/140‑1 (MQ=255)

                                                                                                                    gTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGaa                 <  2:3276004/140‑1 (MQ=255)

                                                                                                                     ttGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTGTTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAg                >  1:4324478/1‑140 (MQ=255)

                                                                                                                       ggTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTc              >  2:2789855/1‑140 (MQ=255)

                                                                                                                       ggTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTc              >  2:2739432/1‑140 (MQ=255)

                                                                                                                       ggTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTc              >  1:2722460/1‑140 (MQ=255)

                                                                                                                       ggTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTc              >  1:1190595/1‑140 (MQ=255)

                                                                                                                       ggTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTc              >  1:2822365/1‑140 (MQ=255)

                                                                                                                         ttttCTCCCTGCGTTAGCGCACAATACGCCACGTTCTTTTTCTCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCGCCCGCCAGACTGGGCGACTGTACCTTTGATGAGCGGGTAGCTGAAGTCtt            <  2:3469732/140‑1 (MQ=255)

                                                                                                                          tttCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTc           <  1:2474045/140‑1 (MQ=255)

                                                                                                                           ttCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCATATCGCCAGACTGGGCGACTGGACCTTTGATGACCGGGTAGCTGAAGTCTTcc          <  1:1870365/140‑1 (MQ=255)

                                                                                                                            tctcCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTCTGTCTCACCGCGACACGCTATTTTCTGCCCCTGTCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTccc         <  1:4658419/140‑1 (MQ=255)

                                                                                                                            tctcCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTccc         <  1:542168/140‑1 (MQ=255)

                                                                                                                            tctcCCTGCGTTAGCGCACAATACGCCAATTTCTTTCTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTCTCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTccc         <  2:4393036/140‑1 (MQ=255)

                                                                                                                                 cTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGata    <  2:3910130/140‑1 (MQ=255)

                                                                                                                                 cTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGata    <  1:2643549/140‑1 (MQ=255)

                                                                                                                                 cTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGata    <  2:2579482/140‑1 (MQ=255)

                                                                                                                                 cTGCATTAGCGCCCACTACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGCCTCACCGCGACCCGCTATTCTCAGCCCCTATCGCCAGCCTCGGCGACTGGACCTTTGATGAACGGGTCGCTGAAGTCTTCCCGGata    <  2:5097659/140‑1 (MQ=255)

                                                                                                                                  tGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGattt   >  2:1698900/1‑138 (MQ=255)

                                                                                                                                  tGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGatat   >  2:2795882/1‑140 (MQ=255)

                                                                                                                                  tGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGGCTTCCCGGataa   >  2:1091192/1‑139 (MQ=255)

ATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATG  >  NZ_CP025268/1957340‑1957610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`