Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,998,341 A→C Y136D (TAT→GAT)  uvrC ← excinuclease ABC subunit UvrC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,998,3410AC98.4% 1410.1 / 8.7 438Y136D (TAT→GAT) uvrCexcinuclease ABC subunit UvrC
Reads supporting (aligned to +/- strand):  ref base A (4/3);  new base C (273/153);  total (281/157)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01

Only 100 of 438 total aligned reads displayed.

CAACGCACGGTCCCAGACAGCGCCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATAGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAGGAAGATAAAAGGATATGATTTATCATCGCGTAGCAAAACGTTGTAACGC  >  NZ_CP025268/1998209‑1998477
                                                                                                                                    |                                                                                                                                        
cAACGCACGGTCCCAGACAGCGCCCTATCTGGGATTGCAGACACGGACGCAAGCGATTGCGATAAACACGATGTTCGCACTGGCGACTGGGGAAAATCTTGTGCATTATCTCGAGTGTTTAACGTACGGCATCTCCAttt                                                                                                                                   >  2:2683878/1‑139 (MQ=255)
     cACGGTCCCAGACAGCGCCCTATCTGGTATTGCAGACAGGGACGCGGGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGTCAGTGTTTCACGTACGGCATCGCCATTCGTGaa                                                                                                                              >  1:2701923/1‑140 (MQ=255)
         gTCCCAGACAGCGCCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACggg                                                                                                                          <  2:4263475/140‑1 (MQ=255)
         gTCCCAGACAGCGCCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACCATTTTCGCACTGGCGAATGGGGAAAATCTTTGGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACggg                                                                                                                          <  2:2493161/140‑1 (MQ=255)
           cccAGAC‑GCGCCCTATCTGCTATTGCAGACACGGACGCGAGCGATTGCGATACACACTATTTTCGCACTGGCGAATGGGGAAACTCTTTTGCAGTAGCGCCAGTGTTCCACGTCCGGCATCGCCCTTCGGGAACGGGCCg                                                                                                                       <  2:2888403/140‑1 (MQ=255)
                   gcgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATAtt                                                                                                                <  1:1146075/140‑1 (MQ=255)
                   gcgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATAtt                                                                                                                <  2:5113175/140‑1 (MQ=255)
                   gcgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATAtt                                                                                                                <  1:5150413/140‑1 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCGCTGGCGATTGGGGAAAACCTTTCGCATTAGCGGCAGTGGTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  2:2111564/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAATATCTTTTGCAGTAGCGCCAGTGTTTCATGTGCGGCATCGCCATTTGGGAACGGGCCGAAATATTc                                                                                                               >  2:1494565/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  1:4242954/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  2:129347/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  1:3360553/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  2:4189604/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  2:3984401/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  2:3777645/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  1:4143145/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  2:1574278/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  1:5068005/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  2:2737142/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  2:1692563/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  1:606187/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  1:4553623/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  1:4638286/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  1:4946568/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  2:447293/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  1:1924813/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  2:771997/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  1:2288773/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  2:4953362/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  1:1046585/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  1:2470920/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  1:2600789/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  1:2816722/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  1:1726463/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  1:2920554/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  1:1488456/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  1:131798/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  1:3011001/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  1:1204325/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  1:313785/1‑140 (MQ=255)
                    cgcCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTCTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTc                                                                                                               >  2:4237590/1‑140 (MQ=255)
                    cgcCCTATCTGGTATGGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGGAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCAGCGCCATTCGGGACCGGGCCGAAATATTc                                                                                                               >  2:2054730/1‑140 (MQ=255)
                        cTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCt                                                                                                           <  1:3596259/140‑1 (MQ=255)
                          aTCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAATGGGCCGAGATATTCACCttt                                                                                                         >  2:2402657/1‑140 (MQ=255)
                             tGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGc                                                                                                      <  1:4349738/140‑1 (MQ=255)
                             tGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGc                                                                                                      <  1:1425731/140‑1 (MQ=255)
                               gTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCAt                                                                                                    >  1:2126365/1‑140 (MQ=255)
                                tATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATg                                                                                                   <  1:2978863/140‑1 (MQ=255)
                                tATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATg                                                                                                   >  1:3057957/1‑140 (MQ=255)
                                 aTTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGc                                                                                                  >  2:342098/1‑140 (MQ=255)
                                 aTTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGc                                                                                                  >  1:1611621/1‑140 (MQ=255)
                                   tGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCtt                                                                                                >  2:1821947/1‑140 (MQ=255)
                                     cAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATTGCCATTCGGGAACGGGCCGAAATATTCACATTTGGCATGCTTCg                                                                                              >  2:898005/1‑140 (MQ=255)
                                     cAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCg                                                                                              >  1:1829775/1‑140 (MQ=255)
                                     cAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCg                                                                                              >  1:3327128/1‑140 (MQ=255)
                                     cAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCg                                                                                              >  1:4803170/1‑140 (MQ=255)
                                       gACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGca                                                                                            <  2:4357155/140‑1 (MQ=255)
                                         cacGGACGCGAGCGAGTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGcacc                                                                                          >  1:704323/1‑140 (MQ=255)
                                          acGGACGCGAGCGATTGCGATAAACACTATTTTTGCACTGGTGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGcacca                                                                                         >  1:3652210/1‑140 (MQ=255)
                                            ggACGCGAGCGATTGCGATAAACACTATTTTTGCACTGGCGAATGGGGGAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGACATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACg                                                                                       >  2:3606415/1‑140 (MQ=255)
                                               cgcgAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATg                                                                                    >  2:3174947/1‑140 (MQ=255)
                                                     cgaTTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGc                                                                              <  2:1970857/140‑1 (MQ=255)
                                                        ttGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAg                                                                           >  1:4485280/1‑140 (MQ=255)
                                                              tAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCgg                                                                     <  2:2993280/140‑1 (MQ=255)
                                                              tAAACACTATTTGCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCCCCTTTGGCATGCTTCGCTCCCCGATGCATCGCCAGTCGCgg                                                                     <  2:369854/140‑1 (MQ=255)
                                                               aaaCACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCggg                                                                    <  2:1924868/140‑1 (MQ=255)
                                                                aaCCCTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCgggt                                                                   <  2:499128/140‑1 (MQ=255)
                                                                  cacTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCgggtgg                                                                 >  1:813177/1‑140 (MQ=255)
                                                                      aTTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTAt                                                             <  2:3343375/140‑1 (MQ=255)
                                                                      aTTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTAt                                                             <  2:2221558/140‑1 (MQ=255)
                                                                       ttttCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATc                                                            >  1:2405044/1‑140 (MQ=255)
                                                                        tttCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATca                                                           <  2:4437988/140‑1 (MQ=255)
                                                                          tCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGGGGGTATcacc                                                         >  2:2855195/1‑140 (MQ=255)
                                                                          tCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGGGGTTCACGTACGGCATCGCCATGCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCTCACCACGAGGCATCGCCAGACGCTGGTGGGTATcacc                                                         >  2:198310/1‑140 (MQ=255)
                                                                           cGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATcacca                                                        >  1:4982792/1‑140 (MQ=255)
                                                                           cGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATcacca                                                        <  2:310541/140‑1 (MQ=255)
                                                                                cggCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAg                                                   <  1:4607476/139‑1 (MQ=255)
                                                                                 ggCGAATGGGGAAAATCTTTTGCAGTAGCGCCCGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAgg                                                  <  2:569317/140‑1 (MQ=255)
                                                                                 ggCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAgg                                                  <  1:471844/140‑1 (MQ=255)
                                                                                 ggCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAgg                                                  <  2:1216727/140‑1 (MQ=255)
                                                                                 ggCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAgg                                                  <  1:3487835/140‑1 (MQ=255)
                                                                                 ggCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAgg                                                  >  2:5201820/1‑140 (MQ=255)
                                                                                            aaaaTCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAGGAAGATAAAAgg                                       >  1:2038345/1‑140 (MQ=255)
                                                                                               aTCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAGGAAGATAAAAGGata                                    <  1:3783935/140‑1 (MQ=255)
                                                                                               aTCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAGGAAGATAAAAGGata                                    <  2:2609322/140‑1 (MQ=255)
                                                                                               aTCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAGGAAGATAAAAGGata                                    <  1:857222/140‑1 (MQ=255)
                                                                                                 cTTTTGCAGTAGCGCCAGTGTTTCGCGTACGGCATCGCCATTCGGGAGCGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAGGAAGATAAAAGGATATg                                  <  2:4530629/140‑1 (MQ=255)
                                                                                                            gcgcCAGTGTTTCACGTACGGCATCGCCATTCGGAAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAGGAAGATAAAAGGATATGATTTATCATcg                       <  2:142269/140‑1 (MQ=255)
                                                                                                              gcCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCTTGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTCTCACCACTCAGGAAGATAAAAGGATATGATTTATCATcgcg                     <  1:2879915/140‑1 (MQ=255)
                                                                                                              gcCAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAGGAAGAGAAAAGGATATGATTTATCATcgcg                     <  1:3941832/140‑1 (MQ=255)
                                                                                                              gcCAGTGGTTCACGTACGGCATCGCCATTCGGGAACCGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAGGAAGATAAAAGGATATGATTTATCATcgcg                     <  1:94070/140‑1 (MQ=255)
                                                                                                               ccAGTGTTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAGGAAGATAAAAGGATATGATTTATCATCGCGt                    >  1:1887277/1‑140 (MQ=255)
                                                                                                                   tgtTTCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAGGAAGATAAAAGGATATGATTTATCATCGCGTAGCa                <  1:3984401/140‑1 (MQ=255)
                                                                                                                     tttCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAGGAAGATAAAAGGATATGATTTATCATCGCGTAGCaaa              <  1:3230126/140‑1 (MQ=255)
                                                                                                                      ttCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATCTTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAGGAAGATAAAAGGATATGATTTATCATCGCGTAGCaaaa             <  2:490479/140‑1 (MQ=255)
                                                                                                                      ttCACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAGGAAGATAAAAGGATATGATTTATCATCGCGTAGCaaaa             <  1:4415646/140‑1 (MQ=255)
                                                                                                                        cACGTACGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAGGAAGATAAAAGGATATGATTTATCATCGCGTAGCAAAACg           >  1:4303883/1‑140 (MQ=255)
                                                                                                                             aCGGCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAGGAAGATAAAAGGATATGATTTATCATCGCGTAGCAAAACGTTGTa      >  2:4689064/1‑140 (MQ=255)
                                                                                                                               ggCATCGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAGGAAGATAAAAGGATATGATTTATCATCGCGTAGCAAAACGTTGTAAc    <  2:4143145/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                        
CAACGCACGGTCCCAGACAGCGCCCTATCTGGTATTGCAGACACGGACGCGAGCGATTGCGATAAACACTATTTTCGCACTGGCGAATGGGGAAAATCTTTTGCAGTAGCGCCAGTGTTTCACGTACGGCATAGCCATTCGGGAACGGGCCGAAATATTCACCTTTGGCATGCTTCGCACCACGATGCATCGCCAGACGCGGGTGGGTATCACCACTCAGGAAGATAAAAGGATATGATTTATCATCGCGTAGCAAAACGTTGTAACGC  >  NZ_CP025268/1998209‑1998477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑