BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,002,534	T→C	329 (TAA→TAG)	dcyD ←	D‑cysteine desulfhydrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,002,534	0	T	C	99.1%	1079.3 / NA	322	329 (TAA→TAG)	dcyD	D‑cysteine desulfhydrase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (155/163); minor base G (1/2); total (157/165)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 322 total aligned reads displayed.

GCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGA  >  NZ_CP025268/2002398‑2002669

  gagaATAAACCCGAGCAGTAAGCCATAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATAATTTCTTGCATGAGCGTTTCCGGAGCTAAAc                                                                                                                                     >  2:4676540/1‑140 (MQ=255)

     aaTAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑g                                                                                                                                 <  2:2460775/140‑1 (MQ=255)

     aaTAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑g                                                                                                                                 <  2:634624/140‑1 (MQ=255)

     aaTAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑g                                                                                                                                 <  1:860388/140‑1 (MQ=255)

      aTAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑gg                                                                                                                                >  1:4108265/1‑140 (MQ=255)

          acacGAGAAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGgat                                                                                                                            <  2:1912492/137‑1 (MQ=255)

            ccGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGgatga                                                                                                                          <  2:302332/140‑1 (MQ=255)

            ccGAGCACTAAGCCAAAAAACATGCCGCCGATACTGAGTTGGAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGgatga                                                                                                                          <  2:4932358/140‑1 (MQ=255)

               aGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAg                                                                                                                       <  2:1855041/140‑1 (MQ=255)

               aGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAg                                                                                                                       <  1:1290866/140‑1 (MQ=255)

                    tAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGaa                                                                                                                  <  1:2743966/140‑1 (MQ=255)

                     aaGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCCGGCACCAGTTGTGTACTTTCCTGCATGCGCGGTTCGGGGCGTAAGCGG‑GGGGAAGATAAGCGatc                                                                                                                 <  2:3918729/140‑3 (MQ=255)

                     aaGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAAc                                                                                                                 <  2:1821620/140‑1 (MQ=255)

                               aCATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGgcg                                                                                                       <  1:2903921/140‑1 (MQ=255)

                                cATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGgcgc                                                                                                      >  2:737241/1‑140 (MQ=255)

                                  tGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGGATGATAGGCGAACAGCGCAGGCGCa                                                                                                     >  2:418636/1‑140 (MQ=255)

                                    ccgccgATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCAccg                                                                                                  <  2:601105/140‑1 (MQ=255)

                                          aTACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTa                                                                                            <  2:4290672/140‑1 (MQ=255)

                                             cTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGa                                                                                         <  2:135454/140‑1 (MQ=255)

                                                   tGCAGCGTATACCCAGCCCCTTACCGCAGGAACGGCAAGGACTCAATAACCAGTTGTCTACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAAc                                                                                   <  2:4077384/140‑1 (MQ=255)

                                                     cAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGTATAAACAg                                                                                 >  1:4868605/1‑140 (MQ=255)

                                                     cAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAg                                                                                 >  2:2091886/1‑140 (MQ=255)

                                                         gTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGGATACTTTCTTGCATGAGCGGTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATc                                                                             <  1:3147316/140‑1 (MQ=255)

                                                          tataCCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCg                                                                            >  1:4345434/1‑140 (MQ=255)

                                                          tataCCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCg                                                                            >  2:494812/1‑140 (MQ=255)

                                                            taCCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAAGAGAATCGGc                                                                          >  2:3552812/1‑140 (MQ=255)

                                                               ccAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCt                                                                       >  1:5145769/1‑140 (MQ=255)

                                                                cAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCtt                                                                      <  2:3689706/140‑1 (MQ=255)

                                                                cAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCtt                                                                      <  2:3463881/140‑1 (MQ=255)

                                                                cAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCtt                                                                      <  1:4253935/140‑1 (MQ=255)

                                                                  gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                    >  2:231297/1‑140 (MQ=255)

                                                                  gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                    >  1:4553545/1‑140 (MQ=255)

                                                                  gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                    >  2:2257888/1‑140 (MQ=255)

                                                                  gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                    >  1:3902435/1‑140 (MQ=255)

                                                                  gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                    >  1:2570576/1‑140 (MQ=255)

                                                                  gCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCa                                                                    >  1:2083680/1‑140 (MQ=255)

                                                                   ccccTTTCAGCAGGAACGGCAAGGAATCACTAACCAGTTGTATACTTTCTTGCATGAGCGTTCCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCAt                                                                   >  2:3890184/1‑140 (MQ=255)

                                                                   ccccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCAt                                                                   >  2:3377987/1‑140 (MQ=255)

                                                                   ccccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCAt                                                                   >  1:2134877/1‑140 (MQ=255)

                                                                    cccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATc                                                                  >  1:3595944/1‑140 (MQ=255)

                                                                    cccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATc                                                                  >  1:1054668/1‑140 (MQ=255)

                                                                       tttCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCttt                                                               >  2:3217188/1‑140 (MQ=255)

                                                                       tttCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCttt                                                               >  1:3322474/1‑140 (MQ=255)

                                                                        ttCAGCCGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATCGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTg                                                              >  1:3473832/1‑140 (MQ=255)

                                                                        ttCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTg                                                              >  2:265977/1‑140 (MQ=255)

                                                                        ttCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTg                                                              >  1:5047143/1‑140 (MQ=255)

                                                                             cagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCg                                                         >  2:1420967/1‑140 (MQ=255)

                                                                             cagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCg                                                         >  1:836714/1‑140 (MQ=255)

                                                                                gAACGGCAAGGAATCAATACCCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGACTAAACAGAATCGGCCCTTCATCTTTGAAGCGttt                                                      <  2:4993042/140‑1 (MQ=255)

                                                                                gAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGttt                                                      <  1:891857/140‑1 (MQ=255)

                                                                                gAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGttt                                                      <  2:5047143/140‑1 (MQ=255)

                                                                                gAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGttt                                                      <  1:4635584/140‑1 (MQ=255)

                                                                                gAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGttt                                                      >  1:1021492/1‑140 (MQ=255)

                                                                                gAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGttt                                                      <  1:3055542/140‑1 (MQ=255)

                                                                                  aCGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTct                                                    <  2:2820460/140‑1 (MQ=255)

                                                                                          gAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATa                                            >  1:1197641/1‑140 (MQ=255)

                                                                                                  aaCCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAAt                                    <  1:1426259/140‑1 (MQ=255)

                                                                                                    ccAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCa                                  >  1:4287787/1‑140 (MQ=255)

                                                                                                     cAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAg                                 >  2:2007897/1‑140 (MQ=255)

                                                                                                       gTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGcc                               <  1:696191/140‑1 (MQ=255)

                                                                                                        ttGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGccc                              >  2:903725/1‑140 (MQ=255)

                                                                                                        ttGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGccc                              >  2:3624820/1‑140 (MQ=255)

                                                                                                             taCTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCa                         >  2:3457895/1‑140 (MQ=255)

                                                                                                              aCTTTCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCAt                        <  1:3650749/140‑1 (MQ=255)

                                                                                                                tttCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCg                      <  1:4985456/140‑1 (MQ=255)

                                                                                                                  tCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                    >  1:4059863/1‑140 (MQ=255)

                                                                                                                  tCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                    >  1:1462695/1‑140 (MQ=255)

                                                                                                                  tCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                    >  2:1262310/1‑140 (MQ=255)

                                                                                                                  tCTTGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                    >  2:165802/1‑140 (MQ=255)

                                                                                                                     tGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGCTGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                 <  1:3440667/140‑1 (MQ=255)

                                                                                                                     tGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                 >  2:2403249/1‑140 (MQ=255)

                                                                                                                     tGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                 >  1:4452233/1‑140 (MQ=255)

                                                                                                                     tGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                 >  2:2605724/1‑140 (MQ=255)

                                                                                                                     tGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                 >  2:1408892/1‑140 (MQ=255)

                                                                                                                     tGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                 >  1:725323/1‑140 (MQ=255)

                                                                                                                     tGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                 <  1:4692223/140‑1 (MQ=255)

                                                                                                                     tGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                 <  1:1987518/140‑1 (MQ=255)

                                                                                                                     tGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                 <  1:3779232/140‑1 (MQ=255)

                                                                                                                     tGCATGAGCGTTTCCGGAGCTAAACGT‑GGGGAGGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                 <  2:307888/140‑1 (MQ=255)

                                                                                                                      gCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTc                <  1:182065/140‑1 (MQ=255)

                                                                                                                      gCATGAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTc                <  2:1783722/140‑1 (MQ=255)

                                                                                                                       cATGAGCGTTTCCGGAGCTAAACGT‑GGAGATGATAGGCGAACAGCGCACGCGCACCGCCGGTATGAATAAACAGAATCGGCCCGTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCTGCGCTTTGcc               >  2:3807242/1‑140 (MQ=255)

                                                                                                                          gAGCGTTTCCGGAGCTAACCGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt            <  1:264967/140‑1 (MQ=255)

                                                                                                                          gAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt            <  2:1573896/140‑1 (MQ=255)

                                                                                                                          gAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt            <  2:1015825/140‑1 (MQ=255)

                                                                                                                          gAGCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt            <  1:4932904/140‑1 (MQ=255)

                                                                                                                          gAGCGTTTCCGGAGCTAAAAGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGCTACCGTCCATCAGCCCCGCCATCGCTTTTCCGGt            <  2:3034572/140‑1 (MQ=255)

                                                                                                                            gCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGtat          >  1:3974637/1‑140 (MQ=255)

                                                                                                                            gCGTTTCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGtat          >  2:1296378/1‑140 (MQ=255)

                                                                                                                            gCGTTTCCGGAGCTAAACGT‑GGGGATGACAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGtat          >  1:452651/1‑140 (MQ=255)

                                                                                                                               tttCCGGAGCTAACCGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATaca       <  2:4516130/140‑1 (MQ=255)

                                                                                                                               tttCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATaca       <  1:231297/140‑1 (MQ=255)

                                                                                                                               tttCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATaca       <  1:2796769/140‑1 (MQ=255)

                                                                                                                               tttCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATaca       <  1:1580395/140‑1 (MQ=255)

                                                                                                                               tttCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATaca       <  2:2201166/140‑1 (MQ=255)

                                                                                                                                ttCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATacac      <  1:2463312/140‑1 (MQ=255)

                                                                                                                                 tCCGGAGCTACACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATAcaca     <  2:1538759/140‑1 (MQ=255)

                                                                                                                                 tCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATAcaca     >  1:3005061/1‑140 (MQ=255)

                                                                                                                                 tCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATAcaca     >  1:4379386/1‑140 (MQ=255)

                                                                                                                                 tCCGGAGCTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATAcaca     <  1:1105453/140‑1 (MQ=255)

GCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGT‑GGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGA  >  NZ_CP025268/2002398‑2002669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`