BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,154,002	T→G	G341G (GGA→GGC)	yegI ←	protein kinase YegI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,154,002	0	T	G	99.6%	794.4 / NA	239	G341G (GGA→GGC)	yegI	protein kinase YegI
Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (78/160); minor base C (1/0); total (79/160)
Fisher's exact test for biased strand distribution p-value = 3.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

CTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCT  >  NZ_CP025268/2153866‑2154137

cTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCg                                                                                                                                      >  1:4486497/1‑140 (MQ=255)

  aaaGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCgag                                                                                                                                    <  1:2560427/140‑1 (MQ=255)

    aGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGAt                                                                                                                                  <  1:2502465/140‑1 (MQ=255)

    aGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGAt                                                                                                                                  <  1:2147078/140‑1 (MQ=255)

        aGCGGGCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAAt                                                                                                                              >  1:1439492/1‑140 (MQ=255)

        aGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAAt                                                                                                                              >  1:782008/1‑140 (MQ=255)

        aGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAAt                                                                                                                              >  2:2376020/1‑140 (MQ=255)

        aGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACGTTCGCCAGCACAAAATTTCAGCCGGTGGTAATGACCTCTTCGACTAGATCAAt                                                                                                                              >  2:2218516/1‑140 (MQ=255)

        aGCGGCCTGCAAGTCGGTTGGAAATGAGCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTGCGCCAGCACAAAATTCCCGCCGGAGGTAATGAGCTCTTCGCCGAGGTCAAt                                                                                                                              >  2:4832875/1‑140 (MQ=255)

         gCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATa                                                                                                                             <  1:2907983/140‑1 (MQ=255)

         gCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATa                                                                                                                             >  1:230658/1‑140 (MQ=255)

         gCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATa                                                                                                                             <  1:1193787/140‑1 (MQ=255)

           ggacTGCTAGTCGGCTTGAAATGATCGGCTAACGCCAGCTCCAATGCTGGCGGGGCTACAGACGCCATCACCATCGCCACGACTTTCGACAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCTAGATCAATaac                                                                                                                           <  2:4314648/137‑2 (MQ=255)

           ggCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAACTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATaaa                                                                                                                           <  2:4185999/140‑1 (MQ=255)

           ggCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTCGTAATGACCTCTTCGCCGAGATCAATaaa                                                                                                                           <  1:3081441/140‑1 (MQ=255)

             ccTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGGTACTGACGCCATCACCATCGCTCAGACTTTCGCGAGCACAAAAATCCCGCCGGT‑GTAATGACCTCTTCGCCGGGAGCAATAAAATa                                                                                                                        >  2:4443774/1‑140 (MQ=255)

                 ccAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGAt                                                                                                                     <  2:103963/140‑1 (MQ=255)

                 ccAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGAt                                                                                                                     >  1:861552/1‑140 (MQ=255)

                 ccAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGAt                                                                                                                     <  1:2632219/140‑1 (MQ=255)

                 ccAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGAt                                                                                                                     <  1:1113454/140‑1 (MQ=255)

                      cGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCCTCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCTAGCTCAATAAAATAGATAacac                                                                                                                <  2:3044043/140‑1 (MQ=255)

                      cGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAacac                                                                                                                <  2:1461799/140‑1 (MQ=255)

                      cGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAacac                                                                                                                <  2:617391/140‑1 (MQ=255)

                        gTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCt                                                                                                              <  1:677105/140‑1 (MQ=255)

                           ggAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGa                                                                                                           <  1:1524461/140‑1 (MQ=255)

                            gAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGAt                                                                                                          >  1:4954835/1‑140 (MQ=255)

                               aTGATCGGGTAATGGCAGTTGCAATGCTGGCCGGGCTACTGACGCCATCACCATCGCCCAGACTTGCGCCAGCACAAAAGTCCCGCCGGTGGTAATTACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTAt                                                                                                       <  2:4734605/140‑1 (MQ=255)

                                tGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATc                                                                                                      <  1:2228438/140‑1 (MQ=255)

                                tGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATc                                                                                                      <  1:454184/140‑1 (MQ=255)

                                tGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATc                                                                                                      <  1:1907451/140‑1 (MQ=255)

                                tGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATc                                                                                                      <  1:3256451/140‑1 (MQ=255)

                                  aTCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCa                                                                                                    <  2:2249741/140‑1 (MQ=255)

                                     gtgTAATGGCAGTTGCAATGCTGGCGGCGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAgcg                                                                                                 <  2:1511285/138‑1 (MQ=255)

                                     gggTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTGTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAgcg                                                                                                 <  1:4314880/140‑1 (MQ=255)

                                      ggTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAgcgc                                                                                                <  2:861552/140‑1 (MQ=255)

                                      ggTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAgcgc                                                                                                <  1:1416835/140‑1 (MQ=255)

                                                 ttGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGc                                                                                     >  1:2392187/1‑140 (MQ=255)

                                                 ttGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGc                                                                                     >  1:3395392/1‑140 (MQ=255)

                                                  tGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACCTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACGTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGca                                                                                    <  2:3110476/140‑1 (MQ=255)

                                                   gCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcag                                                                                   <  2:4136251/140‑1 (MQ=255)

                                                   gCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcag                                                                                   <  1:5046147/140‑1 (MQ=255)

                                                      aTGCTGGCGGTGCTACTCACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcagtca                                                                                <  2:4643367/140‑1 (MQ=255)

                                                       tGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcagtcag                                                                               >  2:2888717/1‑140 (MQ=255)

                                                            gCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcagtca                                                                                >  2:3474800/1‑134 (MQ=255)

                                                            gCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCaaa                                                                          >  1:3792074/1‑140 (MQ=255)

                                                                tGCTCCTGTCGCCAGCCGCCTCGACGAGTCTTTGGCCGTCACAAAATTCCCGCCGTTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAAGACCTTGATTATCCAGCCCACACCGCGGGCAGTCAGTCAAGTGAg                                                                      <  2:1848593/140‑1 (MQ=255)

                                                                tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                      <  1:4396273/140‑1 (MQ=255)

                                                                tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                      <  2:2552230/140‑1 (MQ=255)

                                                                tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                      <  1:3575796/140‑1 (MQ=255)

                                                                tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                      <  1:4244916/140‑1 (MQ=255)

                                                                tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                      <  1:2750268/140‑1 (MQ=255)

                                                                tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                      <  2:3984713/140‑1 (MQ=255)

                                                                tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACATCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                      <  2:1733757/140‑1 (MQ=255)

                                                                tGCTACTGACGCCATCACAATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                      <  2:4244518/140‑1 (MQ=255)

                                                                 gCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGc                                                                     <  1:454232/140‑1 (MQ=255)

                                                                  cTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCg                                                                    >  1:3223597/1‑140 (MQ=255)

                                                                   tACTGACGCCATCACCATCGCCCAGACTTTCACCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCgg                                                                   <  2:693354/140‑1 (MQ=255)

                                                                     cTGACGCCATCATCATCGCCAAGCATTGCGTCAGCACAAAATTCCCCCTGGTCGTAATATCCTCTTCGCCGAGATCAATAAAATACATAACACCTTGAGTATCCAGCGCACACCACGGGCAGTCAGTCAAATCAGCgggg                                                                 <  2:3583042/140‑1 (MQ=255)

                                                                     cTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCgggg                                                                 <  2:3564872/140‑1 (MQ=255)

                                                                      tGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGt                                                                >  1:1878397/1‑140 (MQ=255)

                                                                        aCGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTaa                                                              <  1:1714213/140‑1 (MQ=255)

                                                                          gCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAAc                                                            >  1:298068/1‑140 (MQ=255)

                                                                          gCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGGAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGGCAAATGAGCGGGGTAAAc                                                            >  2:1656739/1‑140 (MQ=255)

                                                                             aTCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATg                                                         >  2:5070019/1‑140 (MQ=255)

                                                                               cACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGCGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGACCGGGGTAAACAtccc                                                       >  1:4062113/1‑137 (MQ=255)

                                                                               cACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCa                                                       >  2:1537553/1‑140 (MQ=255)

                                                                               cACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTAGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCa                                                       >  1:3838247/1‑140 (MQ=255)

                                                                                   aTCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTTGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGc                                                   <  1:5202360/140‑1 (MQ=255)

                                                                                   aTCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGCTCAATCAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGc                                                   <  1:2896408/140‑1 (MQ=255)

                                                                                    tCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCt                                                  >  2:1269619/1‑140 (MQ=255)

                                                                                    tCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCt                                                  >  2:2670392/1‑140 (MQ=255)

                                                                                     cGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTg                                                 >  2:517277/1‑140 (MQ=255)

                                                                                      gCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGa                                                >  2:2720247/1‑140 (MQ=255)

                                                                                            aCTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGAt                                          >  1:370093/1‑140 (MQ=255)

                                                                                            aCTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGAt                                          >  2:3049075/1‑140 (MQ=255)

                                                                                            aCTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCCTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGAt                                          <  1:1990916/140‑1 (MQ=255)

                                                                                                tCGCCAGCACAAAATTCCCGCCGGTGGTACTGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATGCAt                                      <  1:1000160/140‑1 (MQ=255)

                                                                                                      gCACAACATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCttt                                >  1:2420259/1‑140 (MQ=255)

                                                                                                      gCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCttt                                >  2:819137/1‑140 (MQ=255)

                                                                                                      gCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCttt                                <  1:123824/140‑1 (MQ=255)

                                                                                                           aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                           <  2:3155294/140‑1 (MQ=255)

                                                                                                           aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                           <  1:4239598/140‑1 (MQ=255)

                                                                                                           aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                           <  1:1128261/140‑1 (MQ=255)

                                                                                                           aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                           <  2:5033567/140‑1 (MQ=255)

                                                                                                           aaaTTCCCCCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTCATTAACCAGCGCACACCACCGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                           <  2:2843513/140‑1 (MQ=255)

                                                                                                                  cgccgGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGt                    <  2:3658705/140‑1 (MQ=255)

                                                                                                                   gacgGTGGTAATGAACTCTTCGCCGAGATCAATAAAATAGATACCACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTa                   <  1:2816750/138‑1 (MQ=255)

                                                                                                                     ctgttgtAATGACGTCTTCGCCGAGATAAATAAAATAGATACCACCTTGATTATCCAGCGCACACCACGGGCAGTCATTCAAATGAGCGGGGTAAACATACATTGCTCAAACGATACATTTCTTTACCTGTTGGCGTaga                 <  2:1161630/135‑1 (MQ=255)

                                                                                                                     cgGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTaga                 <  1:3059175/140‑1 (MQ=255)

                                                                                                                     cgGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTaga                 <  1:3331549/140‑1 (MQ=255)

                                                                                                                     cgGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTaga                 <  2:4818435/140‑1 (MQ=255)

                                                                                                                          gtAATGACCTCTTCGCCGCGATCAATAAAATAGATAACACCGTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCGGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTc            <  1:343676/140‑1 (MQ=255)

                                                                                                                           tAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTcc           <  1:1549736/140‑1 (MQ=255)

                                                                                                                           tAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTcc           <  2:2345570/140‑1 (MQ=255)

                                                                                                                            aaTGACCTCTTCGCCGAGATCAATAAAATAGATACAAACTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCa          <  1:665940/140‑1 (MQ=255)

                                                                                                                            aaTGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCa          <  1:5123753/140‑1 (MQ=255)

                                                                                                                                  ctctTCGCCGCGATCACTAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCg    >  1:3525741/1‑140 (MQ=255)

                                                                                                                                  ctctTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                           <  1:4386304/117‑1 (MQ=255)

                                                                                                                                  ctctTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCg    <  1:5003162/140‑1 (MQ=255)

                                                                                                                                  ctctTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCg    >  1:396395/1‑140 (MQ=255)

CTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCT  >  NZ_CP025268/2153866‑2154137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`