Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 2,186,098 A→G *322* (TAA→TAG) 
L241L (TTA→CTA) 		 yegV →
yegW ←		 carbohydrate kinase family protein
GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,186,0980AG99.7% 1110.1 / NA 337*322* (TAA→TAG) 
L241L (TTA→CTA) 		yegV
yegW		carbohydrate kinase family protein
GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (165/170);  total (166/171)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 337 total aligned reads displayed.

GCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCG  >  NZ_CP025268/2185961‑2186234
                                                                                                                                         |                                                                                                                                        
gCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGGATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTAGCCCTCGCACACAAAAACGTATAGAt                                                                                                                                        >  2:1161651/1‑140 (MQ=255)
  cGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCg                                                                                                                                      >  2:4399857/1‑140 (MQ=255)
    ggCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCt                                                                                                                                    >  1:606167/1‑140 (MQ=255)
     gCTGGACTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTg                                                                                                                                   <  1:309053/140‑1 (MQ=255)
         gCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGAc                                                                                                                               >  1:3340990/1‑140 (MQ=255)
          cttgtGGTCTGCCACTGGCGGATGCCGTATTACTGCGCAATGCAGTGGCGTCGTGGGTTGTCGGCCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACa                                                                                                                              <  2:2997505/136‑1 (MQ=255)
          ccTCTGGTGTGCCAGTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCTGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACa                                                                                                                              <  2:652006/140‑1 (MQ=255)
          ccTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTTGGTTGTCGGCCATCGGGGCGGTGCTTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAACCGTATAGATCGCTGCGACa                                                                                                                              <  2:1707871/140‑1 (MQ=255)
          ccTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACa                                                                                                                              <  1:366274/140‑1 (MQ=255)
          ccTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACa                                                                                                                              <  1:2542695/140‑1 (MQ=255)
            tctGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGGGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGt                                                                                                                            <  2:3250639/140‑1 (MQ=255)
              tGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTgg                                                                                                                          >  2:4128773/1‑140 (MQ=255)
               ggTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGc                                                                                                                         <  1:3756865/140‑1 (MQ=255)
               ggTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGc                                                                                                                         <  2:4365001/140‑1 (MQ=255)
               ggTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGc                                                                                                                         <  2:5009969/140‑1 (MQ=255)
               ggTCCGCCACTGGCGGATGCCGTATTAATGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCAAACACGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGc                                                                                                                         <  2:196752/140‑1 (MQ=255)
                 tCTGCCACTGGCGGATGTCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTTGCTa                                                                                                                       <  2:3682583/140‑1 (MQ=255)
                 tCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTa                                                                                                                       <  1:2014285/140‑1 (MQ=255)
                 tCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTa                                                                                                                       <  1:1367363/140‑1 (MQ=255)
                 tCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGACGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTa                                                                                                                       <  2:4689411/140‑1 (MQ=255)
                   tGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAAt                                                                                                                     >  1:351316/1‑140 (MQ=255)
                   tGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGGCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACGACTCCTCGCACACAAAAACGGATAGATCGCTGCGACAGGGGCTAAt                                                                                                                     >  1:340799/1‑140 (MQ=255)
                    gCCACTGGCGGATGCCTTATTACTGGGCATGGCAGTGGCGTCTTAGGTTCTCGCGCATCGTGGCGGTGATTGTGCGCCAACTCGCGAGCAACTACTCCTCGCACACAAAAACGGATAGATCGCTGCGACAGTGGCTAATg                                                                                                                    <  2:5160279/140‑1 (MQ=255)
                    gCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATg                                                                                                                    <  2:2107070/140‑1 (MQ=255)
                    gCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATg                                                                                                                    <  1:2641171/140‑1 (MQ=255)
                    gCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATg                                                                                                                    <  1:5064254/140‑1 (MQ=255)
                     ccACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGAGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGc                                                                                                                   >  1:4318600/1‑140 (MQ=255)
                        cTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGt                                                                                                                <  1:1136241/140‑1 (MQ=255)
                         tGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTa                                                                                                               <  1:80592/140‑1 (MQ=255)
                         tGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTa                                                                                                               <  1:575648/140‑1 (MQ=255)
                         tGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTa                                                                                                               <  1:1469092/140‑1 (MQ=255)
                           gCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGAGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACt                                                                                                             >  2:4622478/1‑140 (MQ=255)
                               aTGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAAt                                                                                                         >  1:3878518/1‑140 (MQ=255)
                                     tATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCg                                                                                                   >  2:4686447/1‑140 (MQ=255)
                                     tATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCACCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCg                                                                                                   <  1:4332063/140‑1 (MQ=255)
                                             ggCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCTGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGtt                                                                                           >  1:417705/1‑140 (MQ=255)
                                             ggCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGtt                                                                                           >  2:2180977/1‑140 (MQ=255)
                                             ggCAATGCAGTGGCGTCGTGGGTTGGCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGtt                                                                                           >  2:2441829/1‑140 (MQ=255)
                                               cAATGCAGTGGCGTCGTGGGGTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTgg                                                                                         >  1:2186531/1‑140 (MQ=255)
                                                aaTGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGt                                                                                        <  1:2126710/140‑1 (MQ=255)
                                                  tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCa                                                                                      >  2:660541/1‑140 (MQ=255)
                                                  tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCa                                                                                      >  2:4477261/1‑140 (MQ=255)
                                                  tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCa                                                                                      >  1:4991386/1‑140 (MQ=255)
                                                  tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCa                                                                                      >  2:1261621/1‑140 (MQ=255)
                                                  tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCa                                                                                      >  2:867698/1‑140 (MQ=255)
                                                  tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCa                                                                                      >  1:2051211/1‑140 (MQ=255)
                                                    cAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAg                                                                                    <  2:1585275/140‑1 (MQ=255)
                                                       tGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTg                                                                                                                                   >  2:1870657/1‑90 (MQ=255)
                                                       tGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTg                                                                                                                                   <  1:1870657/90‑1 (MQ=255)
                                                       tGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAgcgc                                                                                 >  1:883452/1‑140 (MQ=255)
                                                       tGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAgcgc                                                                                 >  1:5118680/1‑140 (MQ=255)
                                                       tGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAgcga                                                                                 >  1:4589974/1‑139 (MQ=255)
                                                         gCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCa                                                                               >  1:1069949/1‑140 (MQ=255)
                                                          cgtcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCAc                                                                              <  1:2271532/140‑1 (MQ=255)
                                                            tcgtGTGTTGTCGGGCATCGGGGCGGTGAGTGTGCTCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGTTCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActt                                                                            >  2:1425829/1‑140 (MQ=255)
                                                            tcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGGCAACGCGCGAGGAACTACTCCTCGCACACATAAACGTATAGATCGCTGCGACAGTGGCTAATGAAGTACTCCATAGGCCGTTGCTGTTGGTCAAGCGCCActt                                                                            >  2:3211592/1‑140 (MQ=255)
                                                            tcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGc                                                                                                                         <  2:3959690/95‑1 (MQ=255)
                                                            tcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGc                                                                                                                         >  1:3959690/1‑95 (MQ=255)
                                                            tcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActt                                                                            >  1:2377064/1‑140 (MQ=255)
                                                            tcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActt                                                                            <  2:357890/140‑1 (MQ=255)
                                                            tcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActt                                                                            <  2:1094113/140‑1 (MQ=255)
                                                            tcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGCTGGTCAAGCGCCActt                                                                            >  2:1380659/1‑140 (MQ=255)
                                                                 ggTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCACACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgcttg                                                                       <  1:4875470/140‑1 (MQ=255)
                                                                   ttGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGAt                                                                     >  1:2222051/1‑140 (MQ=255)
                                                                      tCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCAc                                                                  >  2:1892764/1‑140 (MQ=255)
                                                                      tCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCAc                                                                  >  2:2622163/1‑140 (MQ=255)
                                                                      tCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCAc                                                                  >  2:3333017/1‑140 (MQ=255)
                                                                      tCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCAc                                                                  >  2:3303714/1‑140 (MQ=255)
                                                                      tCGGGCATCGGGGCGGGGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCAc                                                                  >  1:2470181/1‑140 (MQ=255)
                                                                            aTCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCAc                                                            <  1:737584/140‑1 (MQ=255)
                                                                             tCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCAcc                                                                 <  1:4413467/134‑1 (MQ=255)
                                                                             tCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCAcc                                                           >  1:518677/1‑140 (MQ=255)
                                                                                gggCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCggg                                                        >  2:3079996/1‑139 (MQ=255)
                                                                                gggCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGt                                                        >  1:4123680/1‑140 (MQ=255)
                                                                                    ggTGAGTGTGCGCCAACGCGCGAGGAACGACTCCTCGCAGACAAGAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTAttt                                                    <  2:756386/140‑1 (MQ=255)
                                                                                      tGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTg                                                  >  2:344424/1‑140 (MQ=255)
                                                                                         ttGTGCGCCAACGCGCGAGGATATACTCCTCGCACACAAACCCGTATAGATCGCTGCGCCAGTCACTAATGCTGTCCTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCCCCGGTATTTTGCTa                                               <  2:1766710/140‑1 (MQ=255)
                                                                                         ttGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTa                                               <  1:3598369/140‑1 (MQ=255)
                                                                                          tgtgCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTAt                                              <  1:4974795/140‑1 (MQ=255)
                                                                                           gtgCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGGCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTGTTTTGCTATc                                             >  2:3805639/1‑140 (MQ=255)
                                                                                            tgCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCt                                            <  2:1285105/140‑1 (MQ=255)
                                                                                            tgCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAACAGGCCGTTGCTGTTGGTCAAGCGCCAATTGCTTGATCACCAGCACCAGTATTTTGCTATCt                                            <  1:4250525/140‑1 (MQ=255)
                                                                                            tgCGCAAACGCGCGAGGAACTGCTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCTATAGGTCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCt                                            <  2:222479/140‑1 (MQ=255)
                                                                                              cgcCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTaa                                          <  1:3302016/140‑1 (MQ=255)
                                                                                              cgcCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTaa                                          >  1:1667355/1‑140 (MQ=255)
                                                                                              cgcCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTaa                                          <  1:1578425/140‑1 (MQ=255)
                                                                                                  accgcgcgTGCAACTACTCCTCGCACACAAAAACGTATACATCGCTGCGACAGTGGCTAATGCTGCACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTCCCTGa                                      <  2:3124292/138‑1 (MQ=255)
                                                                                                    cgcgcgAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAat                                    >  1:3000297/1‑140 (MQ=255)
                                                                                                    cgcgcgAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAat                                    >  2:2800471/1‑140 (MQ=255)
                                                                                                    cgcgcgAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAat                                    >  2:3522152/1‑140 (MQ=255)
                                                                                                    cgcgcgAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAat                                    >  1:1542741/1‑140 (MQ=255)
                                                                                                        cgAGGAACTACTGCTCGCACACAAAAACGTATCGACCGCTGCGACAGTGGCTAATGCTGTACTCAATCGGCCGTTGCTGTTGGTCCAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACGGAATAtgt                                <  2:503474/140‑1 (MQ=255)
                                                                                                           ggAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGAc                             >  2:376533/1‑140 (MQ=255)
                                                                                                                       cGCACACAAAAACGTATAGATCGCTGCGACAGTTGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTTACTGAAACTcggca                 >  2:4432012/1‑140 (MQ=255)
                                                                                                                       cGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATGTAACTGAATATGTGACTGAAACTcggca                 >  1:4758604/1‑140 (MQ=255)
                                                                                                                       cGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggca                 >  2:4840341/1‑140 (MQ=255)
                                                                                                                       cGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggca                 >  2:2581347/1‑140 (MQ=255)
                                                                                                                       cGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATAGGTGACTGAAACTcggca                 >  1:1786652/1‑140 (MQ=255)
                                                                                                                           cacaAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTcggcatccg             <  2:2270183/140‑1 (MQ=255)
                                                                                                                                    gTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCggg    <  2:4891837/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
GCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGAATATGTGACTGAAACTCGGCATCCGGCATCCGGGCG  >  NZ_CP025268/2185961‑2186234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑