BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,229,079	T→C	196 (TAA→TAG)	yohC ←	Yip1 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,229,079	0	T	C	99.6%	876.6 / NA	268	196 (TAA→TAG)	yohC	Yip1 family protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (94/173); total (94/174)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 268 total aligned reads displayed.

CGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCGGA  >  NZ_CP025268/2228943‑2229214

cgcACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCGAGCAATGCAACGTCAAAGAACTTTCTAg                                                                                                                                      >  2:2774561/1‑140 (MQ=255)

cgcACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAg                                                                                                                                      >  1:3217226/1‑140 (MQ=255)

cgcACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAg                                                                                                                                      >  1:3546781/1‑140 (MQ=255)

    ccgccgGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACa                                                                                                                                  <  1:97818/140‑1 (MQ=255)

    ccgccgGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACa                                                                                                                                  <  1:2773284/140‑1 (MQ=255)

      tccgGAGTAAGGAGTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGGCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAAGGCAACGTGAAAGACCTTGCTAGAACaaa                                                                                                                                >  2:3060978/2‑140 (MQ=255)

       ccgGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAAc                                                                                                                               >  2:1123493/1‑140 (MQ=255)

               aGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCg                                                                                                                       >  2:3413342/1‑140 (MQ=255)

               aGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCg                                                                                                                       <  2:4738959/140‑1 (MQ=255)

               aGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCg                                                                                                                       <  1:1706102/140‑1 (MQ=255)

               aGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCg                                                                                                                       >  1:1616378/1‑140 (MQ=255)

                ggATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGt                                                                                                                      <  1:2263699/140‑1 (MQ=255)

                  aTTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAg                                                                                                                    <  1:4007946/140‑1 (MQ=255)

                  aTTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAg                                                                                                                    <  2:3228508/140‑1 (MQ=255)

                  aTTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAg                                                                                                                    <  1:3414816/140‑1 (MQ=255)

                  aTTTACTGAGGCGAGGGACGCCATCATAACGAGCAACAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACACGCGATAACCGTAg                                                                                                                    <  2:4411784/140‑1 (MQ=255)

                     tACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGccc                                                                                                                 <  1:4855333/140‑1 (MQ=255)

                     tACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGccc                                                                                                                 >  1:4142168/1‑140 (MQ=255)

                     tACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGccc                                                                                                                 <  2:1914442/140‑1 (MQ=255)

                     tACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGccc                                                                                                                 <  2:740528/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGCCATCATAACGAGCAACAAGTGCGATCGTCAAAGTAGAATCGCGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATCCAACGTCAAAAAACTTTCTAGAACACACGATAACCGTAGCCCCa                                                                                                               <  2:3777311/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                                                                                                               <  1:3974893/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                                                                                                               <  1:5079875/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                                                                                                               <  2:3086789/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                                                                                                               <  1:2526415/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                                                                                                               <  1:3942493/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                                                                                                               <  2:1295167/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                                                                                                               <  2:475832/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                                                                                                               <  1:3047412/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGACATCATAACGAGAAAAAAGTGCGATCGTCAAAGAAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCa                                                                                                               <  2:1444189/140‑1 (MQ=255)

                       cTGAGGCTAGCGACGAAAGCATAACGAGCAATAAGTGCGATCGTCGAAGGAGAATAGGGAGGAAATGCTGCATTGCTTACATTTACGCCAGCGATGCAAAGTCAAAGAACTGACTATAACAAACGCTAGCCGTAGccccc                                                                                                               <  2:2069307/140‑2 (MQ=255)

                           ggCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAg                                                                                                                    >  2:2259051/1‑131 (MQ=255)

                           ggCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGt                                                                                                           >  2:591306/1‑140 (MQ=255)

                           ggCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGt                                                                                                           >  2:1798928/1‑140 (MQ=255)

                            gCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGta                                                                                                          >  1:2429241/1‑140 (MQ=255)

                             cTAGCGACGCCATCATAACGAGCAAAACGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATACCCGTAGCCCCATAGtat                                                                                                         <  2:4060124/140‑1 (MQ=255)

                             cTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGCAGCGTGACGAAATGCTGCATTGCTGACATTGACGCCACCAATGCAACGTCAAAGAACTTTCTAGCACAAACGATAACCGTAGCCCCAttctat                                                                                                         <  2:1745281/140‑6 (MQ=255)

                             cTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCCTTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGtat                                                                                                         <  2:1533827/140‑1 (MQ=255)

                             cTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGtat                                                                                                         <  1:2935906/140‑1 (MQ=255)

                             cTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGtat                                                                                                         <  2:1201772/140‑1 (MQ=255)

                             cTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGtat                                                                                                         <  2:642027/140‑1 (MQ=255)

                             cTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGtat                                                                                                         <  1:1903335/140‑1 (MQ=255)

                             cTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGtat                                                                                                         <  1:2746558/140‑1 (MQ=255)

                             cTAGCGACGCCATCATAACGAGCAAAAAGTGCGACCGTCAAACGATAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGtat                                                                                                         <  1:1415481/140‑1 (MQ=255)

                             cTAGCGACGCCATCATAACGAGCAAAAAGGGCGATCGTCAAATGTGAATCGTGAGCGACCGCCGCATTGCTCACCTTCACGTCAGTCATCCAACGTACAAGAGCATACTAGAACAATTGATAACCGTAGCCCCATAGtat                                                                                                         <  2:195409/140‑1 (MQ=255)

                               aGCGACGCCATCATCACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATaa                                                                                                       >  1:5054572/1‑140 (MQ=255)

                               aGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATaa                                                                                                       <  1:2239012/140‑1 (MQ=255)

                               aGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATaa                                                                                                       >  1:4546081/1‑140 (MQ=255)

                                      ccATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCg                                                                                                                       <  2:5073213/117‑1 (MQ=255)

                                            tAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGCACTTTCTAGACCAAACGATAACCGTAGCCCCCTAGTATAACGGTTAGGGCGag                                                                                          <  1:2122480/140‑1 (MQ=255)

                                            tAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGag                                                                                          <  2:2920351/140‑1 (MQ=255)

                                             aaCGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGagc                                                                                         <  2:1160767/140‑1 (MQ=255)

                                                gAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGagcagc                                                                                      <  1:542934/140‑1 (MQ=255)

                                                 aGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAgcagca                                                                                     >  1:1514424/1‑140 (MQ=255)

                                                 aGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAgcagca                                                                                     <  1:3501587/140‑1 (MQ=255)

                                                 aGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCAGTGCTGACATTTACGCCAGCAATGAAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTGGGGCGAgcagca                                                                                     <  2:5155252/140‑1 (MQ=255)

                                                 aGCAAAAAGTGCCATCGTCAAAGTAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAAGGTCACCGAACTTTCTAGAACAAACCATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAgcagca                                                                                     <  2:1372924/140‑1 (MQ=255)

                                                   cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACt                                                                                   <  2:2628793/140‑1 (MQ=255)

                                                   cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACt                                                                                   >  2:4623490/1‑140 (MQ=255)

                                                   cAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACt                                                                                   >  1:2695475/1‑140 (MQ=255)

                                                     acaaGTGCGATCGTCAAAGGAGAATCGTGAGGAAACGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTc                                                                                 <  2:2585358/138‑1 (MQ=255)

                                                                cGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCa                                                                      <  2:202647/140‑1 (MQ=255)

                                                                cGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCa                                                                      <  1:1473732/140‑1 (MQ=255)

                                                                cGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACATCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCa                                                                      <  1:2859595/140‑1 (MQ=255)

                                                                      aGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCaa                                                                >  1:3395332/1‑140 (MQ=255)

                                                                           aaTCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGcc                                                           >  1:5212188/1‑140 (MQ=255)

                                                                           aaTCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGcc                                                           <  2:446559/140‑1 (MQ=255)

                                                                           aaTCGTGAGGAAATGCTGCATTGCCGACATTCAAGCCAGCAATGCAACGTCAAAGAACCTGCTAGAACAAACGATAACCGTAGCGCCATAGTATAACGGTTAGGACGAGCAGCACTTCCAGTACCAGCACGACAATCGcc                                                           >  2:1458783/1‑140 (MQ=255)

                                                                             tCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTATCTAGAACAAACGATCACCGTAGCCCCATAGTATAACAGTTAGGGCGAGCAGTACTTCCAGTACCAGCACCCCAATCGcgat                                                         >  2:3633767/1‑137 (MQ=255)

                                                                               gTGAGGAAATGCTGCATTGCTGGCATATACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAAAGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCg                                                       <  2:4082115/140‑1 (MQ=255)

                                                                                         gctgcATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCAGGCCAATCGCCAGCGTCGAACTaga                                             <  1:1221315/140‑1 (MQ=255)

                                                                                         gctgcATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTaga                                             >  2:2229365/1‑140 (MQ=255)

                                                                                         gctgcATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTaga                                             <  1:1554053/140‑1 (MQ=255)

                                                                                         gctgcATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTaga                                             <  2:1586769/140‑1 (MQ=255)

                                                                                          ctgcATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTagag                                            <  1:3775481/140‑1 (MQ=255)

                                                                                          ctgcATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTagag                                            <  2:5030242/140‑1 (MQ=255)

                                                                                          ctgcATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTagag                                            <  1:5111138/140‑1 (MQ=255)

                                                                                               ttGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGACGcc                                       >  2:1918487/1‑139 (MQ=255)

                                                                                                  cTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAg                                    <  2:4284171/140‑1 (MQ=255)

                                                                                                     aCATTTACGCCAGCAATGCAACTGCAAAGAAGTTTCTAGAACAAACGATAACCGTAGCTCGATATTATGACAGTTCGGGCGCGCAGAACTTCCAGTACGAGCACGCCAATCGCCAGGCACGAACTCGCGGATCTAGGGcc                                 >  2:3954082/1‑140 (MQ=255)

                                                                                                       aTTTACGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAGACGATAACCGCAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCtt                               >  1:2870114/1‑140 (MQ=255)

                                                                                                          tACGACAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCCTAGTATAACGGTCAGGGCGACCAGCACTTCCACTACCCGCACGCCAATCGCCAGCCACGAACTAGAGTAGCTAAGCCCATCct                            >  2:2335735/1‑140 (MQ=255)

                                                                                                          ggagCCAGCGAAGCAACTACAAAGTACTGTCTAGAACGAAGGATAACCGTAGCTCAATAGTATCACGGTTAGGGCGAGCAGCACTTCCAGCACCAGCACGCTAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCct                            <  2:2557032/137‑1 (MQ=255)

                                                                                                            cGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCg                                                       >  1:1165756/1‑111 (MQ=255)

                                                                                                            cGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCctct                          >  1:3349318/1‑140 (MQ=255)

                                                                                                            cGCCAGCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCctct                          >  2:2040475/1‑140 (MQ=255)

                                                                                                                 tcAAGGCCACGTCAAAGAACTTACAAGAACAAACGATAACCGTAGCCTCATAGTCTAACGGAAATACCGAGCAGCACTACCAGGACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGTCCTTCctccatggt                     >  2:4684933/2‑134 (MQ=255)

                                                                                                                 gCAATGCAACGTCAAATAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGGTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAGTCGCCAGCGTCGAACTAGAGAAGCTAAGTCCTTCCTCTttgtt                     >  1:4373537/1‑140 (MQ=255)

                                                                                                                 gCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTACGGCCTTCCTCAttgtt                     >  2:2636156/1‑140 (MQ=255)

                                                                                                                 gCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTttgtt                     <  2:926764/140‑1 (MQ=255)

                                                                                                                 gCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTttgtt                     <  2:3435131/140‑1 (MQ=255)

                                                                                                                 gCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTttgtt                     <  2:3122779/140‑1 (MQ=255)

                                                                                                                 gCAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATACTATAACGGTTAGGGCGAGCGGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTttgtt                     <  1:3253666/140‑1 (MQ=255)

                                                                                                                  cAATGCAACGTCAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGtat                                                                                                         <  2:384018/55‑1 (MQ=255)

                                                                                                                          cGTCAAATAACTTTCTAGAACAAACGATAACCGTAGCCCCATCGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCGAAGGCCTTCCTCCTTGTTGATATTCAg            >  1:828844/1‑140 (MQ=255)

                                                                                                                             cAAAGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGaa         <  1:4684066/140‑1 (MQ=255)

                                                                                                                               aaGAACTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGc       >  1:4642830/1‑140 (MQ=255)

                                                                                                                                  aaCTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGCGCACCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCg    <  1:3698339/140‑1 (MQ=255)

                                                                                                                                   aCTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCgg   <  2:1649751/140‑1 (MQ=255)

                                                                                                                                    cTTTCTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCGGa  >  1:4423165/1‑140 (MQ=255)

CGCACCGCCGGAGTAAGGATTTACTGAGGCTAGCGACGCCATCATAACGAGCAAAAAGTGCGATCGTCAAAGGAGAATCGTGAGGAAATGCTGCATTGCTGACATTTACGCCAGCAATGCAACGTCAAAGAACTTTTTAGAACAAACGATAACCGTAGCCCCATAGTATAACGGTTAGGGCGAGCAGCACTTCCAGTACCAGCACGCCAATCGCCAGCGTCGAACTAGAGAAGCTAAGGCCTTCCTCTTTGTTGATATTCAGGAAGCTCGGA  >  NZ_CP025268/2228943‑2229214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`