BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,514,921	A→G	419 (TAA→TAG)	yfeO →	ion channel protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,514,921	0	A	G	99.1%	1146.6 / NA	345	419 (TAA→TAG)	yfeO	ion channel protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (181/161); minor base T (2/1); total (183/162)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 345 total aligned reads displayed.

GCGGCAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGTTTCTG  >  NZ_CP025268/2514784‑2515056

gcggcAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTTCGAAGCAACATCCATCCCATGATAACGTTTAGCa                                                                                                                                       >  2:4505623/1‑140 (MQ=255)

gcggcAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATAGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCa                                                                                                                                       >  2:5129652/1‑140 (MQ=255)

  ggcAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCaaa                                                                                                                                     >  2:1745937/1‑140 (MQ=255)

  ggcAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCaaa                                                                                                                                     <  1:5074050/140‑1 (MQ=255)

         tttGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                              <  2:229277/139‑1 (MQ=255)

         gtcgTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCCGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                              <  1:4322909/137‑1 (MQ=255)

         gTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                              <  1:853097/140‑1 (MQ=255)

         gTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                              <  2:3530119/140‑1 (MQ=255)

         gTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                              <  1:2068103/140‑1 (MQ=255)

         gTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                              <  2:4913253/140‑1 (MQ=255)

         gTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                              <  2:3136228/140‑1 (MQ=255)

           tGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGctct                                                                                                                            >  1:3000239/1‑140 (MQ=255)

                 cAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTa                                                                                                                      >  1:2137927/1‑140 (MQ=255)

                 cAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTa                                                                                                                      >  1:415648/1‑140 (MQ=255)

                  aaTACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGCTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTATCAAAAAATGCTCTCCTTTAt                                                                                                                     >  2:4231623/1‑140 (MQ=255)

                   aTACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                              >  2:1052955/1‑130 (MQ=255)

                    tACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCTGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCACCAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGa                                                                                                                   >  1:2452455/1‑140 (MQ=255)

                     aCCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGAt                                                                                                                  <  1:4025031/140‑1 (MQ=255)

                       cacaTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATCACGTTTAGCAAAAAATGCTCTCCTTTATGAttt                                                                                                                <  1:566425/140‑1 (MQ=255)

                       cacaTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGAttt                                                                                                                <  1:3481161/140‑1 (MQ=255)

                       cacaTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGAttt                                                                                                                <  1:3890185/140‑1 (MQ=255)

                        aggttGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCAGAAGCAACAGCCACCCTACGATGACGTTTAGCAAAAAATGCTCTCCTTTATGATTTa                                                                                                               <  2:699531/137‑1 (MQ=255)

                         caTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                              >  1:4075163/1‑124 (MQ=255)

                         caTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTaa                                                                                                              >  1:4495270/1‑140 (MQ=255)

                         caTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTaa                                                                                                              <  1:1520972/140‑1 (MQ=255)

                         caTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTaa                                                                                                              >  1:3637473/1‑140 (MQ=255)

                         caTTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATCATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGct                                                                                                                              <  2:4075110/124‑1 (MQ=255)

                            tGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTa                                                                                                                      <  1:1026462/129‑1 (MQ=255)

                            tGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTa                                                                                                                      >  2:1026462/1‑129 (MQ=255)

                            tGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAagag                                                                                                           >  2:2090540/1‑140 (MQ=255)

                            tGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAagag                                                                                                           >  2:4949059/1‑140 (MQ=255)

                            tGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAagag                                                                                                           >  1:19188/1‑140 (MQ=255)

                              cTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGtt                                                                                                         >  2:3878887/1‑140 (MQ=255)

                                aCCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTCTTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTAt                                                                                                       >  2:2151000/1‑140 (MQ=255)

                                    ctgctCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCg                                                                                                   >  2:766252/1‑140 (MQ=255)

                                    ctgctCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCg                                                                                                   >  1:4671277/1‑140 (MQ=255)

                                    ctgctCTGTATCGTCATGCTTCCGGCACGGCTGGTATTAGCAGGTAAGCCGATGATGATGGTCAAGCGTCAGAAGCAACAGCCACCCCACGATAAGTATTAGCAAAAAATGCTCTCCTTTATAATTCAAGAGGTATGGCg                                                                                                   >  2:1101792/1‑140 (MQ=255)

                                     tgctCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGGTATGGCga                                                                                                  <  1:3313979/140‑1 (MQ=255)

                                     tgctCTGTATCGTCATGCTGCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTCTGATTTACGAGTTATGGCga                                                                                                  <  2:1212185/140‑1 (MQ=255)

                                     ggctCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCga                                                                                                  <  2:873244/139‑1 (MQ=255)

                                           gTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATAc                                                                                            <  1:5192745/140‑1 (MQ=255)

                                           gTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATAc                                                                                            <  2:2048720/140‑1 (MQ=255)

                                           gTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATAc                                                                                            <  1:1633562/140‑1 (MQ=255)

                                           gTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATAc                                                                                            <  1:3078358/140‑1 (MQ=255)

                                            tATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACg                                                                                           >  1:2531495/1‑140 (MQ=255)

                                            tATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGGCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACg                                                                                           >  2:572516/1‑140 (MQ=255)

                                             aTCGTCATGCTTCCGGGATGGCGGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGCCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAATATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACgg                                                                                          <  1:2874647/140‑1 (MQ=255)

                                             aTCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACgg                                                                                          <  1:289610/140‑1 (MQ=255)

                                               cGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACgggg                                                                                        <  2:3036170/140‑1 (MQ=255)

                                               cGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACgggg                                                                                        <  2:3634233/140‑1 (MQ=255)

                                                 tCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGGAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACggggat                                                                                      >  2:2438242/1‑138 (MQ=255)

                                                 tCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCt                                                                                      >  1:3229915/1‑140 (MQ=255)

                                                    tGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTa                                                                                   >  1:1099962/1‑140 (MQ=255)

                                                     gCTTCCGGCCTGGCTGTTATTATCAGGTAAGCCGTTGATGATGGTCAATCGTCCGACGCGACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTCTGATTTAAGAGTTATGGCGATGATACGGGGCtttcc                                                                                  <  2:457205/140‑3 (MQ=255)

                                                       ttCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCt                                                                                <  2:4462868/140‑1 (MQ=255)

                                                       ttCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCt                                                                                <  2:2640478/140‑1 (MQ=255)

                                                       ttCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCt                                                                                <  1:960649/140‑1 (MQ=255)

                                                         ccGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTcc                                                                              <  1:3569650/140‑1 (MQ=255)

                                                         ccGGCATGGCTGTTATTACCAGGTAAGCCGATGATTATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGTAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGCGCTTTACCTcc                                                                              <  2:3699567/140‑1 (MQ=255)

                                                         ccGCCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACTCTAACGTTTAGCACATAATGCTCTCCTTTATGATTTCAGAGTTATGGCGATGATACGGGGCTTTACCTcc                                                                              <  2:13227/140‑1 (MQ=255)

                                                         acGGCATGGCTGTTATTAACAGGTAAGCCGATGATGATGGTTAATCGTCCGAAGCAACAGCCAACCGACGATAAAGTTTAGCAAAAAAGGGTATCCTTTATGAGTTAAGAGATGGGAGGATGATCCGGGGCCTCACcccc                                                                              >  2:2313184/2‑140 (MQ=255)

                                                           ggCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTcccc                                                                            >  2:3235957/1‑140 (MQ=255)

                                                                ggCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAat                                                                       >  2:3841457/1‑140 (MQ=255)

                                                                 gCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAata                                                                      >  2:4712606/1‑140 (MQ=255)

                                                                 gCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCATTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAata                                                                      >  2:5026775/1‑140 (MQ=255)

                                                                  cTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAatat                                                                     <  1:1745937/140‑1 (MQ=255)

                                                                   tGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATAtt                                                                    >  1:2589649/1‑140 (MQ=255)

                                                                    gTTATTAGCAGGTAAGCAGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAACTGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTg                                                                   <  2:2817966/140‑1 (MQ=255)

                                                                      tattaGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCg                                                                 >  2:822676/1‑140 (MQ=255)

                                                                            cAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACa                                                           >  2:1167296/1‑140 (MQ=255)

                                                                            cAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGGTAACa                                                           >  2:632128/1‑140 (MQ=255)

                                                                            cAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGGTTAGCAAAAAATGCTCTCCTTTATTATGTAAGAGGGATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACa                                                           >  1:2938918/1‑140 (MQ=255)

                                                                               gTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGc                                                        >  1:1899713/1‑140 (MQ=255)

                                                                                  aGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGc                                                     >  1:2336790/1‑140 (MQ=255)

                                                                                  aGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCAACCCCACGATAACGTTTAGCAAAACATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGTGGCTTTACCTCCCCGTAATAAGGCTTTAACATGCCGc                                                     >  2:2387762/1‑140 (MQ=255)

                                                                                      gatgatgatGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGAc                                                 <  2:976367/140‑1 (MQ=255)

                                                                                      gatgatgatGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGAc                                                 <  2:3450109/140‑1 (MQ=255)

                                                                                            gatgGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTc                                           <  1:1241099/140‑1 (MQ=255)

                                                                                              tgGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGa                                         <  2:5108177/140‑1 (MQ=255)

                                                                                                 tCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCg                                      <  1:4877734/140‑1 (MQ=255)

                                                                                                    aTCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGa                                   <  2:2137164/140‑1 (MQ=255)

                                                                                                    aTCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGa                                   <  1:2403825/140‑1 (MQ=255)

                                                                                                        tCCGAAGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGga                               >  1:264884/1‑140 (MQ=255)

                                                                                                          cGAAGCAACAGCCACCCCACGATAACGTTTAGCAACAAATGCCCACTTTGATGATTTAAGAGTGATGGCGACGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGATGGCCTCGAGCGCGCAGgaga                             >  2:3359552/1‑140 (MQ=255)

                                                                                                             aGCAACAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATa                          >  2:2569075/1‑140 (MQ=255)

                                                                                                                aaCAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACa                       <  1:1601650/140‑1 (MQ=255)

                                                                                                                aaCAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACa                       <  1:4585646/140‑1 (MQ=255)

                                                                                                                aaCAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACa                       <  2:2906486/140‑1 (MQ=255)

                                                                                                                aaCAGCCACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACa                       <  2:2424008/140‑1 (MQ=255)

                                                                                                                aaCAGCCACCCCACGATAACGTTTAGACAACAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGTGCTTTACCTCCCCGTACTATGGTGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACa                       <  2:1451921/140‑1 (MQ=255)

                                                                                                                     ccACCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGtt                  <  1:4793157/140‑1 (MQ=255)

                                                                                                                       aCCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCa                >  1:2283231/1‑140 (MQ=255)

                                                                                                                       aCCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCa                <  1:752351/140‑1 (MQ=255)

                                                                                                                       aCCCCACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCa                >  2:4033283/1‑140 (MQ=255)

                                                                                                                       aCCCCACGATAACGTTTAGCAAAAAATGCGCTCCTGTATGATTTAAGAGTTATGGCGATGATACGGGGCTTGACCTCCTCGTAATATTGCGTTAACAGGCCGCTGACGGCTCCGAGCGTGAAGGAGAATCACACTggaca                >  2:1963470/1‑136 (MQ=255)

                                                                                                                         cccACGATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAgg              >  2:549730/1‑140 (MQ=255)

                                                                                                                              gATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACt         >  2:1251361/1‑140 (MQ=255)

                                                                                                                              gATAACGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACt         >  1:4270617/1‑140 (MQ=255)

                                                                                                                                   cGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGTTTc    >  2:2968747/1‑140 (MQ=255)

                                                                                                                                   cGTTTAGCAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGTTTc    >  1:4460493/1‑140 (MQ=255)

GCGGCAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAGCAGGTAAGCCGATGATGATGGTCAATCGTCCGAAGCAACAGCCACCCCACGATAACGTTTAACAAAAAATGCTCTCCTTTATGATTTAAGAGTTATGGCGATGATACGGGGCTTTACCTCCCCGTAATATTGCGTTAACAGGCCGCTGACGGCTTCGAGCGTGAAGGAGAATAACAATGTTCAGGTCACTGTTTCTG  >  NZ_CP025268/2514784‑2515056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`