BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	247,291	A→G	250 (TAA→TAG)	yafL →	C40 family peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	247,291	0	A	G	99.7%	989.1 / ‑5.1	298	250 (TAA→TAG)	yafL	C40 family peptidase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (127/168); total (130/168)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 298 total aligned reads displayed.

GTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGA‑C‑G‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCC  >  NZ_CP025268/247154‑247423

gtgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                                                       <  1:3449619/140‑1 (MQ=255)

gtgtATTTGGGCGATGGGCAATTTATCGAGTCGCAACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                                                       <  1:531624/140‑1 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑c                                                                                                                                     >  1:1074416/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑c                                                                                                                                     >  1:4470087/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑c                                                                                                                                     <  1:4776465/140‑1 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑c                                                                                                                                     >  2:1080755/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑c                                                                                                                                     >  2:1225681/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑c                                                                                                                                     >  2:233134/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑c                                                                                                                                     >  2:4049346/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑c                                                                                                                                     >  1:1497104/1‑140 (MQ=255)

 tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGAGTTTGTAGGA‑c                                                                                                                                     >  2:1325688/1‑140 (MQ=255)

  gtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑C‑g                                                                                                                                   >  1:2319782/1‑140 (MQ=255)

   tATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑C‑gg                                                                                                                                  >  1:1363190/1‑139 (MQ=255)

   tATTTGGGCGATGGGCAATTGATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCGTTCTGGCAGGACCTTTGTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑C‑gg                                                                                                                                  >  1:1937059/1‑139 (MQ=255)

    atgtGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑C‑gga                                                                                                                                 >  1:1777738/4‑138 (MQ=255)

     tttGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑C‑ggat                                                                                                                                <  2:3834633/140‑4 (MQ=255)

       tGGGCGATGGGCATTTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCATGATTTTGACGGAAGAGACGATTTTGTAGGA‑C‑ggataa                                                                                                                              <  2:608377/140‑6 (MQ=255)

       tGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑C‑ggataa                                                                                                                              <  1:1625130/140‑6 (MQ=255)

        gggCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAg                                                                                                                             >  2:4472045/1‑140 (MQ=255)

        gggCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAg                                                                                                                             >  1:600849/1‑140 (MQ=255)

        gggCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAg                                                                                                                             >  2:3777292/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGtt                                                                                                                        >  2:3565940/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGtt                                                                                                                        >  1:382244/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGtt                                                                                                                        >  2:3427937/1‑140 (MQ=255)

             aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATGTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTGGGA‑CGG‑ATAAGGCGtt                                                                                                                        >  1:2883714/1‑140 (MQ=255)

                 gCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTAcg                                                                                                                    <  1:5023123/140‑1 (MQ=255)

                     tttATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGCACCTTTCTGGCAGGACCCTTTTTTGGGCGCGCGCAGGAGTTTGACGGAAGAGGCGAGTTTGTAGGA‑CGG‑ATAAGGCGTTTAcgccgc                                                                                                                >  1:2664767/1‑140 (MQ=255)

                      ttATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCa                                                                                                               <  2:2069743/140‑1 (MQ=255)

                      ttATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCa                                                                                                               <  1:2048691/140‑1 (MQ=255)

                      ttATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCa                                                                                                               <  1:4018621/140‑1 (MQ=255)

                      ttATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTATCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCa                                                                                                               <  1:3701346/140‑1 (MQ=255)

                          cGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCg                                                                                                           >  1:3552299/1‑140 (MQ=255)

                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  2:1949516/140‑1 (MQ=255)

                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  1:555742/140‑1 (MQ=255)

                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  2:434713/140‑1 (MQ=255)

                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  1:5182736/140‑1 (MQ=255)

                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  1:3382537/140‑1 (MQ=255)

                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                          >  1:3180290/1‑140 (MQ=255)

                               cGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGt                                                                                                      >  2:4182225/1‑140 (MQ=255)

                                 ccACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTg                                                                                                    >  2:1799318/1‑140 (MQ=255)

                                   aCGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTa                                                                                                  <  2:102000/140‑1 (MQ=255)

                                       aCCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa                                                                                              <  2:4924313/140‑1 (MQ=255)

                                        ccGGCGACACCATTCGGATAAGCGGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATCTTGCCGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAg                                                                                             <  2:3616619/140‑1 (MQ=38)

                                            cGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGCCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGc                                                                                         <  2:2596370/140‑1 (MQ=255)

                                             gAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGcc                                                                                        <  1:2763633/140‑1 (MQ=255)

                                                aCCATTCGGATAAGCCGATTAACCGAACCGTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCCGTTGTACGCAGGTGCCTGa                                                                                     >  2:1524600/1‑140 (MQ=38)

                                                  cATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATg                                                                                   <  2:3530521/140‑1 (MQ=255)

                                                   aTTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGc                                                                                  >  2:4782269/1‑140 (MQ=255)

                                                       ggATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACg                                                                              <  2:2742005/140‑1 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCCGGATTTTGACGGAAGAGACGATTTTGTAGGA‑C‑GTATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  2:599572/140‑1 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  2:505934/140‑1 (MQ=255)

                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  1:3130746/140‑1 (MQ=255)

                                                               cGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGATGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGcgcg                                                                      <  1:1195916/140‑1 (MQ=255)

                                                                  ttAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCt                                                                   >  2:3237846/1‑140 (MQ=255)

                                                                      ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                               >  1:741649/1‑140 (MQ=255)

                                                                      ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                               <  2:3000192/140‑1 (MQ=255)

                                                                      ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                               <  1:2581345/140‑1 (MQ=255)

                                                                      ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                               <  1:1278434/140‑1 (MQ=255)

                                                                      ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                               <  2:3790647/140‑1 (MQ=255)

                                                                      ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                               <  1:1420690/140‑1 (MQ=255)

                                                                      ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                               <  1:890473/140‑1 (MQ=255)

                                                                      ccGAACCGTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTGGACGGAAGAGAAGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                               <  1:4241268/140‑1 (MQ=37)

                                                                              ttCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTAc                                                       >  2:3033743/1‑140 (MQ=255)

                                                                                cTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGa                                                     >  2:1293407/1‑140 (MQ=255)

                                                                                 tGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAg                                                    <  1:586068/140‑1 (MQ=255)

                                                                                    cAGGACAATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGccc                                                 <  1:2713035/140‑1 (MQ=255)

                                                                                      ggACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCAGCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgc                                               <  2:2538087/140‑1 (MQ=255)

                                                                                        aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGa                                             >  1:625127/1‑140 (MQ=255)

                                                                                         ccATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATACGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGaa                                            <  1:4114225/140‑1 (MQ=255)

                                                                                          cATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAat                                           >  2:3092321/1‑140 (MQ=255)

                                                                                                 tGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCg                                    <  1:1874396/140‑1 (MQ=255)

                                                                                                 tGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCg                                    <  1:3910700/140‑1 (MQ=255)

                                                                                                 tGGGCGAGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCg                                    <  2:1193445/140‑1 (MQ=255)

                                                                                                    gcgcgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGTGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGc                                 >  2:1978044/1‑140 (MQ=37)

                                                                                                      gcgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCg                               >  1:2254704/1‑140 (MQ=255)

                                                                                                       cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGACTGTTTGGGAGcagg                              >  1:1034624/1‑137 (MQ=21)

                                                                                                       cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGc                              <  2:4671552/140‑1 (MQ=255)

                                                                                                       cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGc                              >  2:2912468/1‑140 (MQ=255)

                                                                                                       cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGc                              >  1:3270560/1‑140 (MQ=255)

                                                                                                            aGGATTTTGACGGAAGGGACGAGTTGGTAGGAGT‑G‑AGAAGGCGTTTACGCCGCATCCGGCAGTTGTGCGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  2:3283604/140‑1 (MQ=21)

                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGA‑TGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  2:4300968/140‑1 (MQ=255)

                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  2:5016483/140‑1 (MQ=255)

                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  2:1235925/140‑1 (MQ=255)

                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  1:3038293/140‑1 (MQ=255)

                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  1:5095233/140‑1 (MQ=255)

                                                                                                                ttttGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGat                     <  2:116715/140‑1 (MQ=255)

                                                                                                                ttttGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGat                     <  1:4794317/140‑1 (MQ=255)

                                                                                                                ttttGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGat                     <  1:4597520/140‑1 (MQ=255)

                                                                                                                  ttGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGatat                   <  1:726594/140‑1 (MQ=255)

                                                                                                                      cGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATaaaa               <  1:2021119/140‑1 (MQ=255)

                                                                                                                        gAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaaca             >  1:4367597/1‑140 (MQ=255)

                                                                                                                        gAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGCACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaaca             >  2:1683946/1‑140 (MQ=255)

                                                                                                                         aaGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaacaa            >  2:280849/1‑140 (MQ=255)

                                                                                                                          agagACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaacaac           >  2:693426/1‑140 (MQ=255)

                                                                                                                          agagACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaacaac           >  2:796529/1‑140 (MQ=255)

                                                                                                                                  ttttGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGc   <  1:233801/140‑1 (MQ=255)

                                                                                                                                   tttGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGcc  <  2:3180290/140‑1 (MQ=255)

                                                                                                                                   tttGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGcc  <  1:3433038/140‑1 (MQ=255)

                                                                                                                                   tttGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGcc  <  1:2992104/140‑1 (MQ=255)

                                                                                                                                   tttGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGcc  <  1:2502368/140‑1 (MQ=255)

GTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGA‑C‑G‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCC  >  NZ_CP025268/247154‑247423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`