BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,529,956	T→C	309 (TAA→TAG)	yfeR ←	LysR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,529,956	0	T	C	100.0%	748.4 / NA	221	309 (TAA→TAG)	yfeR	LysR family transcriptional regulator
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (114/107); total (114/107)

Only 100 of 221 total aligned reads displayed.

ACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGG  >  NZ_CP025268/2529822‑2530085

attactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTatat                                                                                                                              >  1:96570‑M2/131‑140 (MQ=255)

 ttactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATc                                                                                                                             <  1:4404177‑M2/11‑1 (MQ=255)

 ttactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATc                                                                                                                             <  2:3216677‑M2/11‑1 (MQ=255)

 ttactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATc                                                                                                                             <  2:2311248‑M2/11‑1 (MQ=255)

    ctacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGa                                                                                                                          >  1:4403412‑M2/127‑140 (MQ=255)

    ctacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGa                                                                                                                          <  2:1304829‑M2/14‑1 (MQ=255)

    ctacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaacttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGa                                                                                                                          <  2:3291078‑M2/14‑1 (MQ=255)

     tacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctcattgattactgccggggctcccggcagtatgaattCACTCTATATCTGAt                                                                                                                         >  1:3587982‑M2/126‑140 (MQ=255)

      acggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATa                                                                                                                        <  1:1685428‑M2/16‑1 (MQ=255)

       cggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATAc                                                                                                                       >  2:1388753‑M2/124‑140 (MQ=255)

         gggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACaa                                                                                                                     <  2:726185‑M2/19‑1 (MQ=255)

          ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACCAc                                                                                                                    >  1:1217916‑M2/121‑140 (MQ=255)

          ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAAc                                                                                                                    >  1:366394‑M2/121‑140 (MQ=255)

          ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAAc                                                                                                                    >  2:3141169‑M2/121‑140 (MQ=255)

          ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAAc                                                                                                                    >  1:3480356‑M2/121‑140 (MQ=255)

          ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAAc                                                                                                                    >  1:2197479‑M2/121‑140 (MQ=255)

            attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttcttattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                  >  1:1195390‑M2/119‑140 (MQ=255)

            attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                  >  1:3938841‑M2/119‑140 (MQ=255)

            attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                  >  2:2860940‑M2/119‑140 (MQ=255)

            attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                  >  2:623461‑M2/119‑140 (MQ=255)

            attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                  >  1:4275605‑M2/119‑140 (MQ=255)

            attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgagtgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACgg                                                                                                                  >  2:5120939‑M2/119‑140 (MQ=255)

              tcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGAt                                                                                                                <  2:3870651‑M2/24‑1 (MQ=255)

                   atcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCt                                                                                                           <  2:436865‑M2/29‑1 (MQ=255)

                    tcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCtt                                                                                                          >  1:4622577‑M2/111‑140 (MQ=255)

                     cgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCCCTCTATATCTGATACAACGGATCCCCTTc                                                                                                         <  1:3998819‑M2/31‑1 (MQ=255)

                     cgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTc                                                                                                         <  1:1401461‑M2/31‑1 (MQ=255)

                      gatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTcc                                                                                                        >  2:4211038‑M2/109‑140 (MQ=255)

                      gatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTcc                                                                                                        <  1:100955‑M2/32‑1 (MQ=255)

                       atatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTccc                                                                                                       >  1:3508938‑M2/108‑140 (MQ=255)

                       atatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTccc                                                                                                       >  1:2897714‑M2/108‑140 (MQ=255)

                       atatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTccc                                                                                                       >  1:3509277‑M2/108‑140 (MQ=255)

                         atgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGc                                                                                                     <  2:4787972‑M2/35‑1 (MQ=255)

                          tgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                                    >  2:766619‑M2/105‑140 (MQ=255)

                          tgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                                    >  1:2103049‑M2/105‑140 (MQ=255)

                           gcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGccc                                                                                                   <  2:1118666‑M2/37‑1 (MQ=255)

                            cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                                  >  1:74324‑M2/103‑140 (MQ=255)

                            cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                                  >  1:4979092‑M2/103‑140 (MQ=255)

                              gatgaaaaactgtgcgaagatttttaccaggatgcgataatctactccatcaaatttaacgtctgattgattactgccgggtctcccggcagtatgaaatCACTCTATATCTTATACAACGGATCCCCTTCCCGCCCAGc                                                                                                >  2:2527823‑M2/101‑140 (MQ=255)

                                 gacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAt                                                                                             >  2:4018651‑M2/98‑140 (MQ=255)

                                   caaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCGATAGCTGATACAACGGATCCCCTTCCCGCCCAGCCATTa                                                                                           <  1:4170385‑M2/45‑1 (MQ=255)

                                      actgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgcg                                                                                        <  1:258730‑M2/48‑1 (MQ=255)

                                        tgggcgaagatttttaccaggatgcgataatctcctccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                      <  1:4538452‑M2/50‑1 (MQ=255)

                                         gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAt                                                                                     >  2:1611630‑M2/90‑140 (MQ=255)

                                         gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAt                                                                                     <  2:572427‑M2/51‑1 (MQ=255)

                                             gaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGcc                                                                                 <  1:835076‑M2/55‑1 (MQ=255)

                                              aagatttttaccagtatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                <  2:3894881‑M2/56‑1 (MQ=255)

                                              aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                <  2:4643579‑M2/56‑1 (MQ=255)

                                              aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                <  2:4177472‑M2/56‑1 (MQ=255)

                                              aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                <  2:3731872‑M2/56‑1 (MQ=255)

                                              aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                <  2:3564592‑M2/56‑1 (MQ=255)

                                              aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                <  2:831054‑M2/56‑1 (MQ=255)

                                              aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                <  2:3480356‑M2/56‑1 (MQ=255)

                                              aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                <  1:903771‑M2/56‑1 (MQ=255)

                                              aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                <  1:1739867‑M2/56‑1 (MQ=255)

                                              aagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                                <  1:3963870‑M2/56‑1 (MQ=255)

                                               agatttttaccaggatgcgataatctactccatcaaatttcacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCt                                                                               <  1:4506102‑M2/57‑1 (MQ=255)

                                               agatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCt                                                                               <  1:4506802‑M2/57‑1 (MQ=255)

                                               agatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCt                                                                               <  2:2467173‑M2/57‑1 (MQ=255)

                                                gatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                              <  1:1118654‑M2/58‑1 (MQ=255)

                                                gatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                              <  1:4745612‑M2/58‑1 (MQ=255)

                                                          aggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTAcc                                                                    <  1:3141169‑M2/68‑1 (MQ=255)

                                                           ggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCa                                                                   >  1:332092‑M2/72‑140 (MQ=255)

                                                             atgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg                                                                 <  1:182069‑M2/71‑1 (MQ=255)

                                                             atgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg                                                                 <  1:2779460‑M2/71‑1 (MQ=255)

                                                             atgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg                                                                 <  1:2983603‑M2/71‑1 (MQ=255)

                                                             atgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg                                                                 <  1:4529111‑M2/71‑1 (MQ=255)

                                                             atgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCGGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg                                                                 <  2:3112664‑M2/71‑1 (MQ=255)

                                                                cgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTcc                                                              >  1:2700699‑M2/67‑140 (MQ=255)

                                                                         actccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCt                                                     >  2:1926829‑M2/58‑140 (MQ=255)

                                                                          ctccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCtt                                                    >  2:264381‑M2/57‑140 (MQ=255)

                                                                           tccatcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTc                                                   >  1:2695359‑M2/56‑140 (MQ=255)

                                                                             catcacatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATAGCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAg                                                 <  2:4583135‑M2/87‑1 (MQ=255)

                                                                             catcaaatttaacttctgattgattcctgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAg                                                 <  2:3954926‑M2/87‑1 (MQ=255)

                                                                             catcaaatttaacttctgattgattactgccggggctcccggcagtatgagttCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGGCCCATAAAGCTTCAg                                                 >  2:4270493‑M2/54‑140 (MQ=255)

                                                                             catcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATCCAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAg                                                 >  2:3064808‑M2/54‑140 (MQ=255)

                                                                             catcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAg                                                 >  2:2128891‑M2/54‑140 (MQ=255)

                                                                               tcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg                                               >  1:1486556‑M2/52‑140 (MQ=255)

                                                                               tcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg                                               <  2:148752‑M2/89‑1 (MQ=255)

                                                                               tcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg                                               >  1:4828266‑M2/52‑140 (MQ=255)

                                                                               tcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg                                               >  2:1567019‑M2/52‑140 (MQ=255)

                                                                               tcaaatttaacttctgattgattactgccggggctcccggcagtatgaattCACGCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCCTAAAGCTTCAgcg                                               >  2:4504916‑M2/52‑140 (MQ=255)

                                                                                             tgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                                 <  1:3392185‑M2/103‑1 (MQ=255)

                                                                                             tgattgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                                 <  2:74579‑M2/103‑1 (MQ=255)

                                                                                               attgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAAc                               >  1:3029532‑M2/36‑140 (MQ=255)

                                                                                               attgattactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAAc                               >  2:3780665‑M2/36‑140 (MQ=255)

                                                                                                    ttactgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttc                          >  1:2332088‑M2/31‑140 (MQ=255)

                                                                                                       ctgccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttcttc                       >  2:3206879‑M2/28‑140 (MQ=255)

                                                                                                          ccggggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTg                    <  2:4466732‑M2/116‑1 (MQ=255)

                                                                                                          ccgcggctcccggcagtatgaattCCCTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTg                    <  2:4324766‑M2/116‑1 (MQ=255)

                                                                                                            ggggctcccggcagtatgaattCACTCTATATCTGAGACAACGGACCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcg                  <  1:5138126‑M2/118‑1 (MQ=255)

                                                                                                             gggctcccggcagtatgaattCGCTCTATATCTGATACAACGGAGCCCCTTCCCGCCCAGGCATCAGCGCATTGCCCTGGTCTCGGACCACGGCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCGTCCtgcgg                 >  2:487580‑M2/22‑139 (MQ=255)

                                                                                                             gggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcgc                 >  2:1064551‑M2/22‑140 (MQ=255)

                                                                                                              ggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCa                <  1:1557403‑M2/120‑1 (MQ=255)

                                                                                                              ggctcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCa                <  1:4956675‑M2/120‑1 (MQ=255)

                                                                                                                 tcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCa             >  2:4948773‑M2/18‑140 (MQ=255)

                                                                                                                 tcccggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCa             <  2:2119562‑M2/123‑1 (MQ=255)

                                                                                                                    cggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCa          >  2:201666‑M2/15‑140 (MQ=255)

                                                                                                                    cggcagtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTGCCTGCGCACCAGCa          <  1:1921055‑M2/126‑1 (MQ=255)

                                                                                                                         gtatgaattCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAAc     <  1:2138279‑M2/131‑1 (MQ=255)

ACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCACACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGG  >  NZ_CP025268/2529822‑2530085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`