BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	306,029	C→T	V786M (GTG→ATG)	ecpC ←	fimbrial usher EcpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	306,029	0	C	T	98.2%	1020.0 / ‑0.9	281	V786M (GTG→ATG)	ecpC	fimbrial usher EcpC
Reads supporting (aligned to +/- strand): ref base C (2/3); new base T (113/162); total (115/166)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

GCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCTCAAT  >  NZ_CP025268/305893‑306167

    cccACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTAtccatgtccatg                                                                                                                                     >  2:3877129/1‑140 (MQ=255)

           tGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACt                                                                                                                              >  1:1421513/1‑140 (MQ=255)

             agagGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCg                                                                                                                            <  2:4749349/140‑1 (MQ=255)

               agGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGcc                                                                                                                          >  1:3322914/1‑140 (MQ=255)

                   cGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGttt                                                                                                                      >  1:3120410/1‑140 (MQ=255)

                     cTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTc                                                                                                                    >  1:1327642/1‑140 (MQ=255)

                     cTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTc                                                                                                                    >  2:5200154/1‑140 (MQ=255)

                       gcggACCACATCACCGACCCAGACGGCACCGCGCGCCGGGTTGACTTCCAGAGCCACTTTGCAGGTTTTATTGCCACTGTAGCGAAACTCGATAGTGGGGTTTTTCTTATCCCTGTCCATGACCAACTCGCCGTTTTCAt                                                                                                                  <  2:2506277/137‑1 (MQ=255)

                       gCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCAt                                                                                                                  <  2:4384433/140‑1 (MQ=255)

                       gCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCAt                                                                                                                  <  2:4051047/140‑1 (MQ=255)

                            ccACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggtt                                                                                                             <  1:1618046/140‑1 (MQ=255)

                               caTCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgg                                                                                                          >  1:1402567/1‑140 (MQ=255)

                               caTCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcgg                                                                                                          >  2:2245100/1‑140 (MQ=255)

                                aTCAGCGACCGAGACGGCACCGCGCGCCTGGCTGAGGGCCAGGGCCACTTCGCAGGCTTTCTTGCCACTGTAGCGAACATCGATGGTGGGGTATTTCTTGTTCACGTCCAACACAAACTCGCCGTTTTCATCggttcggg                                                                                                         <  2:2665529/140‑1 (MQ=255)

                                aTCACCGACCCCGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcggg                                                                                                         >  1:4713194/1‑140 (MQ=255)

                                aTCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTACGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCggttcggg                                                                                                         >  2:1251438/1‑140 (MQ=255)

                                    ccGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTGATCggttcgggttcg                                                                                                     <  2:3524652/140‑1 (MQ=255)

                                     cGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGgttcgggttcgg                                                                                                    >  1:3143553/1‑140 (MQ=255)

                                      gACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGc                                                                                                   <  1:100317/140‑1 (MQ=255)

                                      gACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACCGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGc                                                                                                   <  2:4914058/140‑1 (MQ=255)

                                       aCCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGcc                                                                                                  >  2:2063238/1‑140 (MQ=255)

                                       aCCCAGACGGCACCGCGCGCCTGGTGGAGTTCCAGAGCCACTTCGCAGGGTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTTGGcc                                                                                                  >  2:393961/1‑140 (MQ=255)

                                          cAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGat                                                                                               >  2:3263381/1‑140 (MQ=255)

                                            gACGGCACCGCTCGATTGGTGGCGTTCCAGAGCTACTTCGCAGGTTTTATTGCCGCTGTAGCGAAAATCGTTAGTGGGATATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTGCGGCCGatat                                                                                             <  2:2056645/140‑1 (MQ=255)

                                            gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                             <  2:2886929/140‑1 (MQ=255)

                                            gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                             <  1:849304/140‑1 (MQ=255)

                                            gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                             <  2:2467485/140‑1 (MQ=255)

                                            gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                             <  2:3379375/140‑1 (MQ=255)

                                            gACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCAGatat                                                                                             <  2:1720816/140‑1 (MQ=255)

                                            gACGGCACCGCGCGCCTCGTTGAGTCCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTCTCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                             <  2:3439580/140‑1 (MQ=255)

                                            gACGGCACCGCGCGCCAGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                             <  1:2857951/140‑1 (MQ=255)

                                              cGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATgg                                                                                           <  1:4943799/140‑1 (MQ=255)

                                              cGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATgg                                                                                           <  1:2305723/140‑1 (MQ=255)

                                                  aCCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTATCATCGGTTCGGGTTCGGCCGATATGgttgt                                                                                       >  1:2812570/1‑140 (MQ=255)

                                                  aCCGCGCGCCTGGTTGAGTTCCAGAGCCACCTCGCAGGTTTTATTGCCACTGTAGCGACAATCGATACTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGCTATGgttgt                                                                                       <  2:10737/140‑1 (MQ=255)

                                                      cgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAAt                                                                                   >  2:471093/1‑140 (MQ=255)

                                                      cgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAAt                                                                                   >  1:3305710/1‑140 (MQ=255)

                                                      cgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAAt                                                                                   >  1:2994359/1‑140 (MQ=255)

                                                      cgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAAt                                                                                   >  1:4075451/1‑140 (MQ=255)

                                                      cgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAAt                                                                                   >  2:335101/1‑140 (MQ=255)

                                                      cgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAAt                                                                                   <  2:4447872/140‑1 (MQ=255)

                                                      cgcgcCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCCGCCGATATGGTTGTTAAt                                                                                   >  2:3697051/1‑140 (MQ=255)

                                                        ccccTGGTTCAGTTACAGAGCCACTTTACAGATTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCAGGTCCATGACAAACTCGCCCTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATcc                                                                                 <  2:383909/138‑1 (MQ=255)

                                                           cTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTg                                                                              >  1:488144/1‑140 (MQ=255)

                                                           cTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTg                                                                              <  2:1421513/140‑1 (MQ=255)

                                                             ggTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                            <  1:1791797/140‑1 (MQ=255)

                                                             ggTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                            <  2:5117587/140‑1 (MQ=255)

                                                             ggTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                            <  1:564376/140‑1 (MQ=255)

                                                             ggTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                            <  1:756370/140‑1 (MQ=255)

                                                             ggTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                            <  1:2649187/140‑1 (MQ=255)

                                                             ggTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                            <  2:1922122/140‑1 (MQ=255)

                                                             ggTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCg                                                                            <  2:1845734/140‑1 (MQ=255)

                                                             ggTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCAGATATGGTTGTTAATCCGTGCg                                                                            <  1:3955931/140‑1 (MQ=255)

                                                              gTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGt                                                                           >  1:1459002/1‑140 (MQ=255)

                                                                     tCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcag                                                                    <  1:5042948/140‑1 (MQ=255)

                                                                     tCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcag                                                                    <  1:3400460/140‑1 (MQ=255)

                                                                     cccagccccACTTCGCAGGTTTCCTTGCCACTGTAGCGAAAATCCATACTGGGGTATTTCTTCTCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGCTATGGTTGTTAATCCGTGCGTTAGCcag                                                                    <  2:4209828/133‑1 (MQ=255)

                                                                       cAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcagca                                                                  <  2:3029413/140‑1 (MQ=255)

                                                                       cAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCcagca                                                                  <  1:1131053/140‑1 (MQ=255)

                                                                         gagCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGc                                                                       >  1:105385/1‑133 (MQ=255)

                                                                         gagCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAgt                                                                <  1:3355120/140‑1 (MQ=255)

                                                                              aCTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGcc                                                           <  1:21805/140‑1 (MQ=255)

                                                                              aCTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGcc                                                           <  1:3838156/140‑1 (MQ=255)

                                                                              aCTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGcc                                                           <  1:1505210/140‑1 (MQ=255)

                                                                              aCTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGCATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGTCAGCAGTGTGcc                                                           >  1:4443490/1‑140 (MQ=255)

                                                                                 tCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTc                                                        >  2:2527950/1‑140 (MQ=255)

                                                                                       gTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGc                                                  <  1:2281755/140‑1 (MQ=255)

                                                                                       gTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGc                                                  <  1:5083712/140‑1 (MQ=255)

                                                                                        ttttATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCa                                                 <  2:1741097/140‑1 (MQ=255)

                                                                                        ttttATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCa                                                 <  1:4253770/140‑1 (MQ=255)

                                                                                        ttttATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCa                                                 <  1:2235667/140‑1 (MQ=255)

                                                                                            aTTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGa                                             <  2:1218753/140‑1 (MQ=255)

                                                                                              tGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATa                                           <  1:4859016/140‑1 (MQ=255)

                                                                                              tGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATa                                           <  1:4788520/140‑1 (MQ=255)

                                                                                                 cACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                             >  1:2430478/1‑87 (MQ=255)

                                                                                                 cACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGatat                                                                                             <  2:2430478/87‑1 (MQ=255)

                                                                                                  aCTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGAc                                       >  1:3786011/1‑140 (MQ=255)

                                                                                                  aCTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGAc                                       >  1:99592/1‑140 (MQ=255)

                                                                                                    tGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCg                                     <  1:5150195/140‑1 (MQ=255)

                                                                                                        gccaaaaTCGATACTGGAGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGCTATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCTCACGGATACGACCGgaga                                 <  2:2804888/137‑1 (MQ=255)

                                                                                                            aaaTCGATCGTGGGGTATTTATTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGt                             <  2:2923776/140‑1 (MQ=255)

                                                                                                            aaaTCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGCCCGGAGACGGt                             <  1:2063238/140‑1 (MQ=255)

                                                                                                            aaaTCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGt                             >  1:4163037/1‑140 (MQ=255)

                                                                                                             aaTCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGGGCCGTCTTCCGCACGGATACGACCGGAGACGGTa                            >  2:460326/1‑140 (MQ=255)

                                                                                                              aTCGATAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGtga                           >  2:4334587/1‑138 (MQ=255)

                                                                                                                   tAGTGGGGTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATc                      <  1:817537/140‑1 (MQ=255)

                                                                                                                        gggTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCtt                                                      >  2:3126592/1‑103 (MQ=255)

                                                                                                                         ggTATTTCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTc                <  1:2744714/140‑1 (MQ=255)

                                                                                                                             tttCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCTGCTATAGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTGTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACct            >  2:3100928/1‑140 (MQ=255)

                                                                                                                             tttCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACct            >  2:3565076/1‑140 (MQ=255)

                                                                                                                             tttCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACct            <  2:2994359/140‑1 (MQ=255)

                                                                                                                             tttCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACct            <  1:3876354/140‑1 (MQ=255)

                                                                                                                             tttCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACct            <  1:2527950/140‑1 (MQ=255)

                                                                                                                             tttCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACct            <  2:67376/140‑1 (MQ=255)

                                                                                                                               tCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACctct          <  2:1305641/140‑1 (MQ=255)

                                                                                                                               tCTTATCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACctct          <  1:3556836/140‑1 (MQ=255)

                                                                                                                                   aTCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCt      >  1:5199226/1‑140 (MQ=255)

                                                                                                                                   aTCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCt      >  1:349897/1‑140 (MQ=255)

                                                                                                                                   aTCCATGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCt      >  1:190992/1‑140 (MQ=255)

                                                                                                                                   aTCCATGGCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTAGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCt      >  2:2639342/1‑140 (MQ=255)

GCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCACGTCCATGACAAACTCGCCGTTTTCATCGGTTCGGGTTCGGCCGATATGGTTGTTAATCCGTGCGTTAGCCAGCAGTGTGCCGTCTTCCGCACGGATACGACCGGAGACGGTAACCATCTGCTTCACCTCTGGCTCAAT  >  NZ_CP025268/305893‑306167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`