Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 2,968,493 T→C *749* (TAA→TAG)  ptsP ← phosphoenolpyruvate‑‑protein phosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,968,4930TC99.6% 875.3 / NA 260*749* (TAA→TAG) ptsPphosphoenolpyruvate‑‑protein phosphotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (161/98);  minor base A (0/1);  total (161/99)
Fisher's exact test for biased strand distribution p-value = 3.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

TTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAATGCGCC  >  NZ_CP025268/2968356‑2968629
                                                                                                                                         |                                                                                                                                        
     gTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAAcc                                                                                                                                   <  1:1354067/140‑1 (MQ=255)
         tttCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTcc                                                                                                                               <  1:820980/140‑1 (MQ=255)
         tttCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTcc                                                                                                                               >  1:1951629/1‑140 (MQ=255)
             gCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGa                                                                                                                           <  1:1029434/140‑1 (MQ=255)
             gCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGa                                                                                                                           >  1:601401/1‑140 (MQ=255)
                  aaGAGAACAGCTATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAg                                                                                                                      <  2:825476/140‑1 (MQ=255)
                       aaCAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAgcccgc                                                                                                                 >  2:3739201/1‑140 (MQ=255)
                       aaCAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAgcccgc                                                                                                                 >  2:1332228/1‑140 (MQ=255)
                       aaCAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAgcccgc                                                                                                                 >  1:4410917/1‑140 (MQ=255)
                        aCAGCGATTGAAATGCGCGCCGCAGGTTTCAGTCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAgcccgcc                                                                                                                <  2:935118/140‑1 (MQ=255)
                            cGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTCACAGATCTGGATATGCTCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATg                                                                                                            >  1:4746408/1‑140 (MQ=255)
                              aTTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGcc                                                                                                          >  1:4986496/1‑140 (MQ=255)
                                 gAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGACTAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCcgcg                                                                                                       >  1:4514653/1‑140 (MQ=255)
                                 gAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCACCCCGCCCATGCcgcg                                                                                                       >  2:5146885/1‑140 (MQ=255)
                                 gAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAAGAATAGCACAAGGGGACCTGGCGGGTTGCCGGGTACCGTTAAGAGCTATGTATATGACCCGCGCTATAACCCTCCGCGGAGCAGCCCGCCCATGcttcg                                                                                                       >  2:2681782/1‑136 (MQ=255)
                                  aaaTGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGa                                                                                                      <  1:1682667/140‑1 (MQ=255)
                                    aTGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACg                                                                                                    <  2:1025772/140‑1 (MQ=255)
                                    aTGCGCGCCGCAGGTTGCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACg                                                                                                    <  2:1794403/140‑1 (MQ=255)
                                     tGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGc                                                                                                   <  2:4741594/140‑1 (MQ=255)
                                     tGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGc                                                                                                   <  1:4343601/140‑1 (MQ=255)
                                     tGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGc                                                                                                   <  2:1769690/140‑1 (MQ=255)
                                     tGCGCGCCGCAGGTCTCAGGCGCTACAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGc                                                                                                   <  1:4398998/140‑1 (MQ=255)
                                         cgccgcAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCa                                                                                               >  1:4873160/1‑140 (MQ=255)
                                         cgccgcAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCa                                                                                               >  2:4514093/1‑140 (MQ=255)
                                         cgccgcAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCa                                                                                               >  2:2757799/1‑140 (MQ=255)
                                            ctcAGGTTTCAGGCGCTCCAAAGTTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTAGATGATCCGCGCTATAACCCTACGCGAATCAGCCCGCCCATGCCGCGACGCTCCATaa                                                                                            <  2:4016145/138‑1 (MQ=255)
                                            cgcAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATaa                                                                                            >  1:3237850/1‑140 (MQ=255)
                                            cgcAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATaa                                                                                            <  1:2602986/140‑1 (MQ=255)
                                            cgcAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATaa                                                                                            >  1:2478428/1‑140 (MQ=255)
                                                      aGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAAc                                                                                  >  1:3502636/1‑140 (MQ=255)
                                                         cgcTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTg                                                                               <  2:3216666/140‑1 (MQ=255)
                                                           cTCCAAAGGTGCGAATAATAGCACAAGGGGATCTGGCTGGTTGCCGGATACCGGTAAAAGATAGGTATCTGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAACGCTGCCACCTGaa                                                                             >  2:2427248/1‑139 (MQ=255)
                                                           cTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGAt                                                                             >  2:2904797/1‑140 (MQ=255)
                                                                    tGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACATGATGGCGAACtt                                                                    <  2:686632/140‑1 (MQ=255)
                                                                     gCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTc                                                                   >  1:771294/1‑140 (MQ=255)
                                                                      cGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGCACTTCg                                                                  >  1:648401/1‑140 (MQ=255)
                                                                      cGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCg                                                                  >  1:1524760/1‑140 (MQ=255)
                                                                      cGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCg                                                                  >  2:1855328/1‑140 (MQ=255)
                                                                      cGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCg                                                                  >  2:332732/1‑140 (MQ=255)
                                                                      cGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCg                                                                  >  2:3642875/1‑140 (MQ=255)
                                                                      cGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCg                                                                  >  1:2903605/1‑140 (MQ=255)
                                                                      cGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCCCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCg                                                                  <  1:3806661/140‑1 (MQ=255)
                                                                      cGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGACCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCg                                                                  >  2:3119432/1‑140 (MQ=255)
                                                                        aataatAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGt                                                                >  2:4802534/1‑140 (MQ=255)
                                                                             taGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGGTACGTATGTGCTCCGCGCTATGACCCTCCGCTGAGCAGCCCGCCCATGCCGCGACGCTCCATAAAAGCTGCACCCTGATGTCGAACTTCGTTCGCCa                                                           >  2:343886/1‑140 (MQ=255)
                                                                             gaGCACTAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCGGCGCTATAACCCTCCGCGAATCAGCCCGGCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATTGCGAAATTCGGTCTccg                                                           >  2:2594023/2‑139 (MQ=255)
                                                                                cacaAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGtt                                                        >  1:2775252/1‑140 (MQ=255)
                                                                                 acaAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTg                                                       <  2:773010/140‑1 (MQ=255)
                                                                                 acaAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTg                                                       <  1:3903038/140‑1 (MQ=255)
                                                                                 acaAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTg                                                       <  1:5036031/140‑1 (MQ=255)
                                                                                 acaAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTg                                                       <  2:1055598/140‑1 (MQ=255)
                                                                                   aaGGGGACCTGGCTGGTTGCCGGCTACCGTTAAAAGATATGTATTTGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCCACCTGATGGCGAACTTCGGTCGCCAGTTgcg                                                     <  2:2935909/140‑1 (MQ=255)
                                                                                    aGGGGACCTGGAGGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATACCCCTCCGCGAATCAGCCCGCCCATGCCGCTACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTgcgc                                                    <  2:503979/140‑1 (MQ=255)
                                                                                     ggggACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCt                                                   >  1:4200558/1‑140 (MQ=255)
                                                                                     ggggACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCt                                                   >  1:2259497/1‑140 (MQ=255)
                                                                                      gggACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCtt                                                  <  2:1595361/140‑1 (MQ=255)
                                                                                      gggACCTGGCTGGTTCCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCtt                                                  <  2:1520062/140‑1 (MQ=255)
                                                                                         aCCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATACGCGCTATAACCCTCCTCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCa                                               <  2:3976812/140‑1 (MQ=255)
                                                                                          ccTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAg                                              >  1:1717843/1‑140 (MQ=255)
                                                                                          ccTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAg                                              >  1:966285/1‑140 (MQ=255)
                                                                                           ctggctggTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCCTAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGa                                             <  2:1951629/140‑1 (MQ=255)
                                                                                             ggctggTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACt                                           >  1:405260/1‑140 (MQ=255)
                                                                                               ctggTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTCCCAGACTAc                                         >  1:144658/1‑140 (MQ=255)
                                                                                                tggTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACg                                        <  1:1633780/140‑1 (MQ=255)
                                                                                                  gTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCt                                      >  2:1362188/1‑140 (MQ=255)
                                                                                                  gTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCt                                      >  1:2964272/1‑140 (MQ=255)
                                                                                                       cGGATACCGTTAAAAGATATGTGTATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCa                                 <  1:3749101/140‑1 (MQ=255)
                                                                                                        ggATACCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCaa                                >  1:286624/1‑140 (MQ=255)
                                                                                                            aCCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCa                                                                                                              >  1:3587486/1‑58 (MQ=255)
                                                                                                            aCCGTTAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGAtt                            >  1:4594808/1‑140 (MQ=255)
                                                                                                              cGTTAAAAGATATGTATATGATCCGCGCTATAACCCACCGCGAATCAGCCCGCCCGTGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGAtttt                          <  2:3831783/140‑1 (MQ=255)
                                                                                                                ttAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGCCTACGCTGCGCAAGATTTTCt                        >  1:510045/1‑140 (MQ=255)
                                                                                                                ttAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCt                        >  2:2083801/1‑140 (MQ=255)
                                                                                                                ttAAAAGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCt                        >  2:4045934/1‑140 (MQ=255)
                                                                                                                   aaaGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCt                     <  2:2289185/140‑1 (MQ=255)
                                                                                                                   aaaGATATGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCt                     <  1:2083801/140‑1 (MQ=255)
                                                                                                                   aaaGATATGGAGACGATCCGCGATATACCCCTCCGCGCATCAGCCCGCCCATGCCGCGACGCTCCATAACCGCTGCCACTTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCACACTACGCTGCGCAAGATTTTCTggt                     <  2:3015216/140‑3 (MQ=255)
                                                                                                                        tatGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGACCTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGc                <  1:310932/140‑1 (MQ=255)
                                                                                                                        tatGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTAGGCTGCGCAAGATTTTCTGCTTCGGc                <  2:4663165/140‑1 (MQ=255)
                                                                                                                         atGTATATGATCCGCGCTATAACCCTCCGCGAATCATCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGGTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCCGCa               >  1:715015/1‑140 (MQ=255)
                                                                                                                          tGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCAt              >  1:4568433/1‑140 (MQ=255)
                                                                                                                          tGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCAt              >  2:3869776/1‑140 (MQ=255)
                                                                                                                          tGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCAt              >  2:414607/1‑140 (MQ=255)
                                                                                                                          tGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCAt              >  2:2372540/1‑140 (MQ=255)
                                                                                                                          tGTATATGATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCAt              >  2:1750316/1‑140 (MQ=255)
                                                                                                                                 gATCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAAt       >  1:1788798/1‑140 (MQ=255)
                                                                                                                                 gATCAGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGAAGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAAt       >  2:4986713/1‑140 (MQ=255)
                                                                                                                                  aTCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAATg      >  1:543626/1‑140 (MQ=255)
                                                                                                                                   tCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATg                                                                                                            <  1:4001450/37‑1 (MQ=255)
                                                                                                                                   tCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAATgc     >  1:1968346/1‑140 (MQ=255)
                                                                                                                                   tCCGCGCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAATgc     >  2:114296/1‑140 (MQ=255)
                                                                                                                                     cgcgCTATAACCCTCCTCGAATCAGCCCGCCCATACCGCGCCGCTCAATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCCGGAGAAGCAAAgcgc   >  2:1705931/1‑140 (MQ=255)
                                                                                                                                     cgcgCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGTAAGACTTTCTGCTCCGGCCTACTCACTgagc   >  2:4628656/1‑140 (MQ=255)
                                                                                                                                     cgcgCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCt                     >  1:1671727/1‑122 (MQ=255)
                                                                                                                                     cgcgCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCt                     <  2:1671593/122‑1 (MQ=255)
                                                                                                                                     cgcgCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAATgcgc   >  1:3323947/1‑140 (MQ=255)
                                                                                                                                     cgcgCTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAATgcgc   >  1:3919703/1‑140 (MQ=255)
                                                                                                                                      gcgcTATAAGCCTCCGCGAATCAGACCGCCAATGCCGCGACGCACCAAAAAGGCTGAAACCAGATGGCGAAATTCGGTCGCCTGCTGCGGTTCCAGGTTACGCTGCGCAAGATTTTCTGCTTCGGTATGAACAATCcgcc  >  2:1987259/1‑140 (MQ=255)
                                                                                                                                      gcgcTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATGTTCTGCTTCGGCATAATCAATGcgcc  >  2:4263286/1‑140 (MQ=255)
                                                                                                                                      gcgcTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATGTTCTGCTTCGGCATAATCAATGcgcc  >  1:1998462/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
TTCTTGTTATTTCGCTGAAAGAGAACAGCGATTGAAATGCGCGCCGCAGGTTTCAGGCGCTCCAAAGGTGCGAATAATAGCACAAGGGGACCTGGCTGGTTGCCGGATACCGTTAAAAGATATGTATATGATCCGCGTTATAACCCTCCGCGAATCAGCCCGCCCATGCCGCGACGCTCCATAAAGGCTGCAACCTGATGGCGAACTTCGGTCGCCAGTTGCGCTTCCAGACTACGCTGCGCAAGATTTTCTGCTTCGGCATAATCAATGCGCC  >  NZ_CP025268/2968356‑2968629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑