BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,985,593	T→C	279 (TAA→TAG)	kduI ←	5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,985,593	0	T	C	99.7%	976.5 / NA	291	279 (TAA→TAG)	kduI	5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (150/140); minor base A (0/1); total (150/141)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 291 total aligned reads displayed.

TCACAGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGC  >  NZ_CP025268/2985457‑2985728

tCACAGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAg                                                                                                                                      >  1:4957977/1‑140 (MQ=255)

   caGCACGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCAAGAGAAACTGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACCACTAgcgc                                                                                                                                   <  2:1425507/140‑1 (MQ=255)

      cccGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCaaa                                                                                                                                >  1:4929421/1‑140 (MQ=255)

      cccGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCaaa                                                                                                                                >  1:1524115/1‑140 (MQ=255)

          cTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCtt                                                                                                                            >  2:687743/1‑140 (MQ=255)

          cTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCtt                                                                                                                            >  1:3513643/1‑140 (MQ=255)

          cTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCtt                                                                                                                            >  2:3590762/1‑140 (MQ=255)

           ttGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCttt                                                                                                                           <  2:2267949/140‑1 (MQ=255)

             gcgcCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTACAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTaa                                                                                                                         <  2:3064073/140‑1 (MQ=255)

               gcCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAGATCTTTAACg                                                                                                                       <  2:1604280/140‑1 (MQ=255)

                   gCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACACCCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTACAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCca                                                                                                                   <  1:3209598/140‑1 (MQ=255)

                    ccccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATCCCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcac                                                                                                                  <  1:213339/140‑1 (MQ=255)

                    ccccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcac                                                                                                                  <  1:4054841/140‑1 (MQ=255)

                     ccccACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTAACGCCCGCAACTTTACCTTCGAGACAAAATGCACTTAAAATCATAAGCCTGCCTTATTCGTTTATGCCCACGACTAGCGCAAATCTTTAACGGCcaca                                                                                                                 <  2:3861686/140‑1 (MQ=255)

                     cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                 <  1:4880375/140‑1 (MQ=255)

                     cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                 <  2:1466976/140‑1 (MQ=255)

                     cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                 <  2:2342130/140‑1 (MQ=255)

                     cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                 <  1:1932330/140‑1 (MQ=255)

                     cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                 <  2:403894/140‑1 (MQ=255)

                     cccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                 <  1:111905/140‑1 (MQ=255)

                     cccAACGCCACCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATCCCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                 <  2:2384343/140‑1 (MQ=255)

                      ccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACAt                                                                                                                <  2:3969106/140‑1 (MQ=255)

                         aCGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGt                                                                                                             <  1:1982282/140‑1 (MQ=255)

                               tCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCatat                                                                                                       >  1:911248/1‑140 (MQ=255)

                                      aCCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCaaa                                                                                                >  2:3767775/1‑140 (MQ=255)

                                         cagtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                             <  2:1891334/140‑1 (MQ=255)

                                         cagtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGAACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATAGTCCATATCATCAAAGAc                                                                                             <  2:1397440/140‑1 (MQ=255)

                                           gtccagtATCACAACCAGTGACGACCGCAACTTTACTTTTGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAACTCTTTAACGGCCACATGGTCGATATCATCAAAGAcat                                                                                           >  2:1863768/1‑138 (MQ=255)

                                           gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCt                                                                                           >  1:507793/1‑140 (MQ=255)

                                           gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCt                                                                                           >  1:4183586/1‑140 (MQ=255)

                                            tccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTg                                                                                          >  2:2888783/1‑140 (MQ=255)

                                            tccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTg                                                                                          >  2:916105/1‑140 (MQ=255)

                                            tccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTACATATCATCAAAGACCTg                                                                                          >  2:5146105/1‑140 (MQ=255)

                                            tccagtATCACAACCAGTGACGACCGCAACTTTACCTTAGAGCGAAAATGCACTTAAAATCATAACAATACCTTATTCGATTATGCCCACAACTAGCGCAAATCTTTCACGGCCACATGGTCCATATCATCAAAGACTTg                                                                                          >  2:336871/1‑140 (MQ=255)

                                             ccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTgg                                                                                         <  2:3173923/140‑1 (MQ=255)

                                                 tATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGtttt                                                                                     >  1:1027508/1‑140 (MQ=255)

                                                 tATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGtttt                                                                                     >  2:1286695/1‑140 (MQ=255)

                                                 tATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGtttt                                                                                     >  1:1693745/1‑140 (MQ=255)

                                                    cacaACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCAc                                                                                  <  1:696952/140‑1 (MQ=255)

                                                     acaACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCAcc                                                                                 >  1:809025/1‑140 (MQ=255)

                                                      caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCg                                                                                >  2:747830/1‑140 (MQ=255)

                                                      caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCg                                                                                >  1:2063420/1‑140 (MQ=255)

                                                      caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCg                                                                                >  2:3926683/1‑140 (MQ=255)

                                                      caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCg                                                                                >  2:4154760/1‑140 (MQ=255)

                                                      caACCAGTGACGACCGCAACTTTACCGTCGAGAGAAAATGCCCTGAAAATCATATCATTACCGTATTCGTTTGTGCCGACAACTAGCGCAAAGCTTTAACGGCACCATGGTCCATATCATCAAATACCTCGCTTTCACCg                                                                                >  2:3271243/1‑140 (MQ=255)

                                                         ccAGTGACGACCGCAACTTTACCTTCCAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGAcc                                                                             <  2:4884129/140‑1 (MQ=255)

                                                          cAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCa                                                                            <  1:364139/140‑1 (MQ=255)

                                                           aGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCag                                                                           >  1:23669/1‑139 (MQ=255)

                                                           aGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCAt                                                                           >  1:4556983/1‑140 (MQ=255)

                                                            gTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCcaca                                                                                                                 <  1:1456201/101‑1 (MQ=255)

                                                            gTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATg                                                                          >  2:2893330/1‑140 (MQ=255)

                                                             tGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGc                                                                         >  1:355661/1‑140 (MQ=255)

                                                             tGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCAATATCATCAAAGGCCTGGTTTTCCAAGACCATGc                                                                         >  2:3802690/1‑140 (MQ=255)

                                                              gacgacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGGCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                        <  1:4432043/140‑1 (MQ=255)

                                                              gacgacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                        <  1:567490/140‑1 (MQ=255)

                                                               acgacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGccc                                                                       <  1:1563935/140‑1 (MQ=255)

                                                                 gacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCa                                                                     >  2:2794457/1‑140 (MQ=255)

                                                                   ccGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGa                                                                   >  2:407246/1‑140 (MQ=255)

                                                                   ccGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGa                                                                   >  2:4872442/1‑140 (MQ=255)

                                                                    cGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGAt                                                                  >  1:2997769/1‑140 (MQ=255)

                                                                    cGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGAt                                                                  >  1:2244322/1‑140 (MQ=255)

                                                                    cGCAACTTTACCTTCGAGAGAAAACGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTCACGGCCACATGGTCCATATCATCACAGACCTGGTTTTCACCGACCATGCCCCAGAt                                                                  <  2:2041143/140‑1 (MQ=255)

                                                                     gCAACTTTACCTTCGAGAGAAAATGCACTTAACATCACAACCATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATa                                                                 <  1:3072743/140‑1 (MQ=255)

                                                                     gCAACTTTACCGTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATa                                                                 <  1:3436160/140‑1 (MQ=255)

                                                                      cAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATaa                                                                >  2:5046477/1‑140 (MQ=255)

                                                                          tttACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGt                                                            <  2:64982/140‑1 (MQ=255)

                                                                           ttACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGta                                                           <  2:2023014/140‑1 (MQ=255)

                                                                             aCCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGtata                                                         >  2:4731402/1‑140 (MQ=255)

                                                                             aCCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGtata                                                         <  1:3387697/140‑1 (MQ=255)

                                                                                 tCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCt                                                     >  2:2076005/1‑140 (MQ=255)

                                                                                   gagagaAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCttt                                                   >  2:458835/1‑140 (MQ=255)

                                                                                         aaaTGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTcc                                             <  1:2516275/140‑1 (MQ=255)

                                                                                             gCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                         <  1:895933/140‑1 (MQ=255)

                                                                                             gCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                         <  2:5171288/140‑1 (MQ=255)

                                                                                             gCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                         <  1:2909225/140‑1 (MQ=255)

                                                                                             gCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                         <  1:512151/140‑1 (MQ=255)

                                                                                              cACTTAAATTCATCACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGacac                                        <  2:4267124/140‑1 (MQ=255)

                                                                                               aCTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACAcc                                       >  1:4705969/1‑140 (MQ=255)

                                                                                               aCTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACAcc                                       >  1:4707162/1‑140 (MQ=255)

                                                                                                  tAAAATCATAACAATACCTTATTCGTTTACGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGa                                    <  2:3644997/140‑1 (MQ=255)

                                                                                                   aaaaTCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGaa                                   >  1:1855938/1‑140 (MQ=255)

                                                                                                           aaCAATACCTTATTCGTTTATGCCCACACCTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGTTCGa                           <  2:4807569/140‑1 (MQ=255)

                                                                                                             cAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGAcc                         <  1:2815708/140‑1 (MQ=255)

                                                                                                                tACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCCAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGc                      >  2:3429838/1‑140 (MQ=255)

                                                                                                                tACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGc                      >  2:3636731/1‑140 (MQ=255)

                                                                                                                       ttCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGga               >  1:2558235/1‑140 (MQ=255)

                                                                                                                            ttATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCa          >  1:3899738/1‑140 (MQ=255)

                                                                                                                            ttATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCa          >  1:741719/1‑140 (MQ=255)

                                                                                                                               tGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCg       >  2:1394999/1‑140 (MQ=255)

                                                                                                                               tGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCg       >  1:4644017/1‑140 (MQ=255)

                                                                                                                                gCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGc      <  1:927909/140‑1 (MQ=255)

                                                                                                                                 cccACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGcc     >  1:2317770/1‑140 (MQ=255)

                                                                                                                                 cccACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGcc     <  1:4657028/140‑1 (MQ=255)

                                                                                                                                  ccACAACTGGCGTAAATCTTTAACAGCCACCTGGGCCATATCATCAAAGACCTGGTTTTCACCGACCAGGCCCCTGATAAAGGTATAAGCTTTGGTTCCGTCACCGGAATCGATCGACCAGCTCGGGGAGATCACCGCCt    <  2:1251099/140‑1 (MQ=255)

                                                                                                                                  ccACAACTAGCGCAAATCTTTCACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCt    <  2:3243789/140‑1 (MQ=255)

                                                                                                                                  ccACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCt    <  2:1216014/140‑1 (MQ=255)

                                                                                                                                   cacaACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTg   <  2:569109/140‑1 (MQ=255)

                                                                                                                                   cacaACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTg   <  2:4173831/140‑1 (MQ=255)

                                                                                                                                    acaACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGc  >  2:4930574/1‑140 (MQ=255)

                                                                                                                                    acaACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGc  >  2:3489362/1‑140 (MQ=255)

TCACAGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGC  >  NZ_CP025268/2985457‑2985728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`