BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	311,241	T→C	G26G (GGT→GGC)	CXP41_RS01610 →	protein YkgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	311,241	0	T	C	99.1%	760.3 / ‑6.0	233	G26G (GGT→GGC)	CXP41_RS01610	protein YkgL
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (104/127); minor base G (2/0); total (106/127)
Fisher's exact test for biased strand distribution p-value = 2.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 233 total aligned reads displayed.

GTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAAT  >  NZ_CP025268/311105‑311375

  ttttATGTTAGTACAAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGAGCCGCAAAGGTAGGAGACTGAGCGGCGTGCTCAGTCTAAAAGAAGGATGATGCCAGCATACAGGCGGGAAGc                                                                                                                                   >  2:4157801/1‑140 (MQ=255)

   tttATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGTGTTGATGCCAGCATACAGGGGCGAAGCt                                                                                                                                  >  1:1682208/1‑140 (MQ=255)

   tttATGTTAGTACCAACAGATTTACCACAGTTATCATCTTAAGAGTTGGAGTGAAAATGCAGGCTGCGCCGCAAAGGGTGGAGACGGAGTGTCGTGTTCAGTGTAAAAGAGGATTGATGCCAGCATACAGGGGCGAGGcg                                                                                                                                  >  2:3080198/1‑139 (MQ=255)

              aCCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGt                                                                                                                       <  2:2463772/140‑1 (MQ=255)

               ccAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTc                                                                                                                      <  2:1837016/140‑1 (MQ=255)

               ccAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTc                                                                                                                      <  1:4410696/140‑1 (MQ=255)

               ccAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTc                                                                                                                      <  1:4956988/140‑1 (MQ=255)

               ccAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTc                                                                                                                      <  1:3705472/140‑1 (MQ=255)

               ccAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTc                                                                                                                      <  2:3497874/140‑1 (MQ=255)

               ccAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTc                                                                                                                      <  1:505785/140‑1 (MQ=255)

               ccAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTc                                                                                                                      <  2:1169946/140‑1 (MQ=255)

                 aaCAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCaa                                                                                                                    <  2:2253481/140‑1 (MQ=255)

                    aGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACAt                                                                                                                 <  1:2471041/140‑1 (MQ=255)

                    aGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACAt                                                                                                                 <  1:376051/140‑1 (MQ=255)

                    aGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACAt                                                                                                                 <  2:390849/140‑1 (MQ=255)

                      aTTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTa                                                                                                               >  1:1720654/1‑140 (MQ=255)

                       tttACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTaa                                                                                                              >  2:4884120/1‑140 (MQ=255)

                       tttACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTaa                                                                                                              <  2:1627714/140‑1 (MQ=255)

                       tttACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTaa                                                                                                              >  2:20468/1‑140 (MQ=255)

                           ccACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATa                                                                                                          <  1:2395624/140‑1 (MQ=255)

                             acaTTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAAt                                                                                                        >  1:542432/1‑140 (MQ=255)

                             acaTTTATCATCTTAACAGTTTGAGGGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAAt                                                                                                        >  2:4664087/1‑140 (MQ=255)

                              caTTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATg                                                                                                       >  1:4749923/1‑140 (MQ=255)

                              caTTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATg                                                                                                       >  1:2516771/1‑140 (MQ=255)

                                tttATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGGTGATGCCAGCATACAGGGGCGAAGCTGGTCAAAAAGTCAACATTAAGATAATGGa                                                                                                     >  2:3357490/1‑140 (MQ=255)

                                     catcTTAATAGGTAGGGTGAAAATGCAGACTGCCCCGCAAAGTGAGGAGCATGAGTCGCATGTACAGTCTCAAAGAGCTTTGAAGGCAGCATACAAGGGCAACGCTAATCAACAAGTCAACATTAAGAAAATGTAATAtt                                                                                                >  2:4970439/1‑140 (MQ=255)

                                        cTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAATATAGGGTTGATGGCAGCATACATGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGGATATTCgg                                                                                             >  2:2713608/1‑140 (MQ=255)

                                         ttAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGga                                                                                            >  2:3939579/1‑140 (MQ=255)

                                         ttAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGga                                                                                            >  1:820583/1‑140 (MQ=255)

                                           aaCAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGgaga                                                                                          >  2:241416/1‑140 (MQ=255)

                                           aaCAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGgaga                                                                                          >  2:5197333/1‑140 (MQ=255)

                                            aCAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGgagag                                                                                         <  1:3956482/140‑1 (MQ=255)

                                            aCAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGgagag                                                                                         <  1:1968197/140‑1 (MQ=255)

                                                tttGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAAc                                                                                     >  2:4806220/1‑140 (MQ=255)

                                                  tGAGTGAAAATGCAGGCTGCGCCGCATAGGGAGGAGACTGAGTGGCGTGTTCAGTCTACAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGGCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGt                                                                                   >  2:1521487/1‑140 (MQ=255)

                                                  tGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGt                                                                                   >  2:1427267/1‑140 (MQ=255)

                                                  tGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGGTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGt                                                                                   >  2:3462888/1‑140 (MQ=255)

                                                    aggcaaaaTGCAGGCTGCGCCGCACAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCCGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCa                                                                                 <  1:2621829/136‑1 (MQ=255)

                                                      tGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAgg                                                                               <  1:2432090/140‑1 (MQ=255)

                                                         aaaTGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAg                                                                            <  2:2661338/140‑1 (MQ=255)

                                                              cAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGc                                                                       <  1:4208768/140‑1 (MQ=255)

                                                              cAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGc                                                                       <  2:4552033/140‑1 (MQ=255)

                                                              cAGGCTGCGCCGCAAAGGGAGGAGACTGAGGGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCAGACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGc                                                                       >  2:4710575/1‑140 (MQ=255)

                                                                ggCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAGAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTGGGAGAGAAACGTCAGGCAGTGGGcgt                                                                     >  2:4402756/1‑138 (MQ=255)

                                                                  cTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCa                                                                   >  1:549318/1‑140 (MQ=255)

                                                                      gccgcAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATg                                                               >  2:1480408/1‑140 (MQ=255)

                                                                         gcAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAc                                                            <  1:420335/140‑1 (MQ=255)

                                                                            aaGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAgg                                                         <  1:5162477/140‑1 (MQ=255)

                                                                            aaGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAgg                                                         <  1:4632132/140‑1 (MQ=255)

                                                                            aaGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAgg                                                         <  1:4066768/140‑1 (MQ=255)

                                                                             aGGGAGGAGACTGAGTGTCGTGTTCCGTCTAAAAGAGGGTTGATGCCAGCATACAGGGCCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGga                                                        <  2:3435209/140‑1 (MQ=255)

                                                                             aGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGga                                                        <  2:5124608/140‑1 (MQ=255)

                                                                             aGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAAAAAGTCAACAGTAAGATAATGAAATATGCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAAAGga                                                        <  2:5125309/140‑1 (MQ=255)

                                                                                    agaCTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAatat                                                 <  1:1549039/140‑1 (MQ=255)

                                                                                      aCTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAatatat                                               >  1:561283/1‑140 (MQ=255)

                                                                                         gAGTTGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTGAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATccc                                            >  2:929402/1‑140 (MQ=255)

                                                                                         gAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGTATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGAGAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATAGccc                                            >  2:327275/1‑140 (MQ=255)

                                                                                         gAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATccc                                            >  1:3850937/1‑140 (MQ=255)

                                                                                            tGGCGTGTTCAGGCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAg                                         <  2:719842/140‑1 (MQ=255)

                                                                                              gCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGa                                       >  1:325133/1‑140 (MQ=255)

                                                                                               cGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGgagaga                                                                                        >  1:93131/1‑90 (MQ=255)

                                                                                               cGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGaa                                      >  1:115878/1‑140 (MQ=255)

                                                                                                gtgtTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGaaa                                     >  2:4006376/1‑140 (MQ=255)

                                                                                                    tCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTa                                 >  2:1711533/1‑140 (MQ=255)

                                                                                                        tCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAACATTAATGt                             <  2:561283/140‑1 (MQ=255)

                                                                                                        tCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGt                             >  1:311843/1‑140 (MQ=255)

                                                                                                        tCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGt                             >  2:2807827/1‑140 (MQ=255)

                                                                                                          tAAAAGAGGGTTGATTCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTg                           <  2:653846/140‑1 (MQ=255)

                                                                                                          tAAAAGAGGGTTGATGCCAGCATACAGGGTCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGCTGGCCTCAAATCAACAGGAAGAATATATCCCCAGGAAAATTAATGTTg                           <  2:1877353/140‑1 (MQ=255)

                                                                                                          tAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGACACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTg                           <  2:282404/140‑1 (MQ=255)

                                                                                                          tAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTg                           <  1:4239927/140‑1 (MQ=255)

                                                                                                          tAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTg                           <  1:914425/140‑1 (MQ=255)

                                                                                                          tAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTg                           <  1:3635368/140‑1 (MQ=255)

                                                                                                          tAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTg                           <  2:3648569/140‑1 (MQ=255)

                                                                                                          tAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTg                           <  1:2155554/140‑1 (MQ=255)

                                                                                                          tAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTg                           <  1:102690/140‑1 (MQ=255)

                                                                                                          tAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTg                           <  1:241416/140‑1 (MQ=255)

                                                                                                          tAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAACTAATGGAACATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTg                           <  2:1379696/140‑1 (MQ=255)

                                                                                                            aaaGAGGGTTGATGCCAGCATACAGGGGCGAAGGTGGTCAACAAGTCGACATTAAGATAATGTAATAGTCGGGGAGAAACGTCAGGCAGGTGGCCTGAAATGAATAGGGAGTATAGACCAGCAGTAAAGTTAAtggtggg                         >  2:4080151/1‑139 (MQ=255)

                                                                                                             aaGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGgtg                        >  1:3390157/1‑140 (MQ=255)

                                                                                                                  ggTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGc                                                                       >  2:1271187/1‑88 (MQ=255)

                                                                                                                  ggTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGc                                                                       <  1:1271187/88‑1 (MQ=255)

                                                                                                                  ggTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTtata                   >  1:1767358/1‑140 (MQ=255)

                                                                                                                  ggTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTtata                   >  2:5047194/1‑140 (MQ=255)

                                                                                                                  ggTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTtata                   >  2:1988436/1‑140 (MQ=255)

                                                                                                                  ggTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTtata                   >  2:2280453/1‑140 (MQ=255)

                                                                                                                    ttGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATaaa                 <  1:3887796/140‑1 (MQ=255)

                                                                                                                    ttGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATaaa                 <  2:2241410/140‑1 (MQ=255)

                                                                                                                       aTGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacac              >  1:2269917/1‑140 (MQ=255)

                                                                                                                         gCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacacac            <  2:3869359/140‑1 (MQ=255)

                                                                                                                         gCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacacac            <  1:4005556/140‑1 (MQ=255)

                                                                                                                         gCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATAATCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGCAAAATTAATGTTGGTGTTATAAacacac            <  1:2733933/140‑1 (MQ=255)

                                                                                                                         gCCAGCATACAGGGGCGAAGCTAGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGACCAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacacac            <  2:4992354/140‑1 (MQ=255)

                                                                                                                          ccAGCATACATGGGCGAAGCTCGTCAACAAGTCATCATTAAGATAATGGAATATTTGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACTGGAAGAATATATCCCCAGGATAATTAATGTTGGTGTTATAAACACAcc           <  2:3801750/140‑1 (MQ=255)

                                                                                                                          ccAGCATACAGGGGCGCAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAGTATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACAcc           <  1:2872129/140‑1 (MQ=255)

                                                                                                                          ccAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGTAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACAcc           <  2:744365/140‑1 (MQ=255)

                                                                                                                          ccAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACAcc           <  1:3521470/140‑1 (MQ=255)

                                                                                                                            aGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCaa         >  2:3933054/1‑140 (MQ=255)

                                                                                                                             gCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAAc        >  1:2619551/1‑140 (MQ=255)

                                                                                                                                   aGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAAt  >  2:1738703/1‑140 (MQ=255)

GTTTTTATGTTAGTACCAACAGATTTACCACATTTATCATCTTAACAGTTTGAGTGAAAATGCAGGCTGCGCCGCAAAGGGAGGAGACTGAGTGGCGTGTTCAGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGTGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAAT  >  NZ_CP025268/311105‑311375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`