BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,034,336	A→G	S222S (TCA→TCG)	uacT →	urate/proton symporter UacT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,034,336	0	A	G	99.7%	996.2 / NA	299	S222S (TCA→TCG)	uacT	urate/proton symporter UacT
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (179/119); minor base T (0/1); total (179/120)
Fisher's exact test for biased strand distribution p-value = 4.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 299 total aligned reads displayed.

CCGCAATATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAATCATCG  >  NZ_CP025268/3034199‑3034469

    aaTATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATATTGCTCATTACTCGCTATGCGAAAGGATTTATGTACAACGTCGCCGTATTACTGGGGATTGTATTTGGCCTTTTACTTTCGTGgatg                                                                                                                                 <  1:1753321/140‑1 (MQ=255)

         ggTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTTTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGatgaa                                                                                                                            <  1:2528781/140‑1 (MQ=255)

          gTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGCATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGatgaat                                                                                                                           <  2:4627849/140‑1 (MQ=255)

           tAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGatgaatg                                                                                                                          >  1:280936/1‑140 (MQ=255)

             aTCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGAtgaatgaa                                                                                                                        >  1:260746/1‑140 (MQ=255)

             aTCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGAtgaatgaa                                                                                                                        <  2:1097837/140‑1 (MQ=255)

              tCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAg                                                                                                                       >  1:2875833/1‑140 (MQ=255)

              tCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAg                                                                                                                       >  1:707386/1‑140 (MQ=255)

                   tttatttaGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAg                                                                                                                       <  1:4718636/135‑1 (MQ=255)

                   tttatttaGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAAt                                                                                                                  >  1:3571799/1‑140 (MQ=255)

                   tttatttaGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAAt                                                                                                                  >  1:4197821/1‑140 (MQ=255)

                   tttatttaGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAAt                                                                                                                  >  1:5066932/1‑140 (MQ=255)

                   tttatttaGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAAt                                                                                                                  >  1:74036/1‑140 (MQ=255)

                       tttaGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTAt                                                                                                              <  1:453058/140‑1 (MQ=255)

                        ttaGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATc                                                                                                             <  1:1493339/140‑1 (MQ=255)

                            gTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGCTTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCggg                                                                                                         >  2:2205957/1‑140 (MQ=255)

                            gTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCggg                                                                                                         >  2:808165/1‑140 (MQ=255)

                                 tcCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACa                                                                                                    <  1:5199135/140‑1 (MQ=255)

                                   cTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACatg                                                                                                  <  2:2317586/140‑1 (MQ=255)

                                   cTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACatg                                                                                                  <  2:3503806/140‑1 (MQ=255)

                                   cTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACatg                                                                                                  <  2:58174/140‑1 (MQ=255)

                                   cTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACatg                                                                                                  <  1:107498/140‑1 (MQ=255)

                                     ttGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACatgat                                                                                                >  2:2796916/1‑140 (MQ=255)

                                      tGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACatgatg                                                                                               >  1:1276577/1‑140 (MQ=255)

                                       gCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGc                                                                                              <  2:4153011/140‑1 (MQ=255)

                                           tCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCa                                                                                          <  2:889533/140‑1 (MQ=255)

                                           tCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCa                                                                                          >  1:602718/1‑140 (MQ=255)

                                           tCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCa                                                                                          >  1:3671946/1‑140 (MQ=255)

                                           tCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCa                                                                                          <  2:2983551/140‑1 (MQ=255)

                                           tCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCa                                                                                          >  2:2961138/1‑140 (MQ=255)

                                           tCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCa                                                                                          >  1:64830/1‑140 (MQ=255)

                                           tCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCa                                                                                          >  1:1660211/1‑140 (MQ=255)

                                           tCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCa                                                                                          >  2:1373239/1‑140 (MQ=255)

                                           tCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCa                                                                                          >  2:1196745/1‑140 (MQ=255)

                                             ttAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATg                                                                                        >  1:3462939/1‑140 (MQ=255)

                                             ttAAGTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATg                                                                                        >  2:4777400/1‑140 (MQ=255)

                                                   tttATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGc                                                                                  <  1:305694/140‑1 (MQ=255)

                                                     tATCTTGCTCATTACTCGCTATGCGAAAGGATTTATTTCCAACGTCGCCGTATTACTGGGGATTGTATGTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCGTCAGGGTTTGCGa                                                                                >  1:528073/1‑140 (MQ=255)

                                                     tATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGa                                                                                <  1:2142554/140‑1 (MQ=255)

                                                     tATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGa                                                                                <  2:76957/140‑1 (MQ=255)

                                                     tATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGa                                                                                <  1:3252224/140‑1 (MQ=255)

                                                         ttGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGt                                                                            <  2:4041088/140‑1 (MQ=255)

                                                                ttACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCg                                                                     <  2:233163/140‑1 (MQ=255)

                                                                 tACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGa                                                                    >  2:1889868/1‑140 (MQ=255)

                                                                      gCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATCAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCg                                                               >  1:2896003/1‑140 (MQ=255)

                                                                           gCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTgg                                                          >  2:2395276/1‑140 (MQ=255)

                                                                           gCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTgg                                                          >  1:4378861/1‑140 (MQ=255)

                                                                           gCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTgg                                                          >  1:4254528/1‑140 (MQ=255)

                                                                           gCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTGGGCCCTTTAGTTTCGTGGATGATGAGTGAAGTCCATTTATCCGCGCTACATCATGCTTCATGAATTACGATCGTTACGCCGATGTCGTTTgg                                                          >  2:3418113/1‑140 (MQ=255)

                                                                                  gATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCg                                                   >  2:504839/1‑140 (MQ=255)

                                                                                  gATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCg                                                   >  2:3956827/1‑140 (MQ=255)

                                                                                  gATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCg                                                   <  1:1953901/140‑1 (MQ=255)

                                                                                    tttATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACGTGATGCTTCATGGTTTGCGATTGTTACGCCGATATCGTTTGGTATGCCGAt                                                 >  2:4971790/1‑140 (MQ=255)

                                                                                    tttATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGAt                                                 >  1:271212/1‑140 (MQ=255)

                                                                                    tttATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGAt                                                 >  2:3208942/1‑140 (MQ=255)

                                                                                    tttATGTCCAACGTCGCCGTATTACTGGGGATTGCATTTGGCTTTTTACTTTCGTGGCTGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGAt                                                 >  1:3629177/1‑140 (MQ=255)

                                                                                            cAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATc                                         >  2:3874641/1‑140 (MQ=255)

                                                                                            cAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATc                                         >  1:2481486/1‑140 (MQ=255)

                                                                                            cAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATc                                         >  1:5011149/1‑140 (MQ=255)

                                                                                            cAACGTCGCCGTATTACTGGGAATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGAGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATc                                         >  2:2623310/1‑140 (MQ=255)

                                                                                            cAACGTAGCCGTATTACCGGGGAGGGTATTTGGCTTTTTGCTTGCGTGGATGATGAATGGAGTCAATTTATCAGGGCTACATTATGATTCATGGTTTGCGATTGTTACGCTGACGTCGTTTGGTATGCCGATTTTCGATc                                         >  2:1247685/1‑140 (MQ=255)

                                                                                             aaCGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCTGATTTTCGATcc                                        <  2:5145734/140‑1 (MQ=255)

                                                                                             aaCGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATcc                                        <  2:470371/140‑1 (MQ=255)

                                                                                                  cGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGttt                                   >  2:3283014/1‑140 (MQ=255)

                                                                                                  cGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGttt                                   >  1:4574672/1‑140 (MQ=255)

                                                                                                  cGCCGTAGTACGGGGGGTTGTATGTGTCTTTTTACTTTCGGGGAGGAGGAATGAAGTCAAGTTATCTGGTCTACATGTTGCGTCATGGTTTGCGATTGTTACGCCGGTGTCGGTTGGTAGGTCGATTTTCGGTcgctgtt                                   >  2:1636852/1‑134 (MQ=255)

                                                                                                    ccGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTcc                                 >  1:1357535/1‑140 (MQ=255)

                                                                                                       tATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCAtt                              <  2:4740265/140‑1 (MQ=255)

                                                                                                       tATTACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCAtt                              <  2:4773325/140‑1 (MQ=255)

                                                                                                          tACTGGTGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTg                           <  1:3845254/140‑1 (MQ=255)

                                                                                                          tACTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGGCGATTTTCGATCCCGTTTCCATTCTg                           <  1:4408860/140‑1 (MQ=255)

                                                                                                            cTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGAc                         <  1:3133498/140‑1 (MQ=255)

                                                                                                            cTGGGGATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGAc                         <  1:4900770/140‑1 (MQ=255)

                                                                                                              ggggATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACatga                                                                                                 <  2:4339177/66‑1 (MQ=255)

                                                                                                               gggATTTGATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAGTTTCTCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTAGGCCGATTTTCGATCCCGTTTCCATTCTGACCAt                      >  2:1997215/1‑140 (MQ=255)

                                                                                                               gggATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCAt                      <  2:260746/140‑1 (MQ=255)

                                                                                                               gggATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCAt                      >  2:1618478/1‑140 (MQ=255)

                                                                                                               gggATTGTATTTGGCTTGTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCAt                      >  2:2076915/1‑140 (MQ=255)

                                                                                                                 gATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACa                                                                                                    >  2:253633/1‑60 (MQ=255)

                                                                                                                 gATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACa                                                                                                    <  1:253633/60‑1 (MQ=255)

                                                                                                                 gATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATTATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGa                    >  1:2184922/1‑140 (MQ=255)

                                                                                                                 gATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTTACCATGa                    >  2:4320295/1‑140 (MQ=255)

                                                                                                                 gATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGa                    >  1:2061839/1‑140 (MQ=255)

                                                                                                                 gATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGa                    >  2:4484427/1‑140 (MQ=255)

                                                                                                                 gATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGa                    >  1:3512402/1‑140 (MQ=255)

                                                                                                                 gATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGa                    >  1:2678149/1‑140 (MQ=255)

                                                                                                                 gATTGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGa                    >  2:4858207/1‑140 (MQ=255)

                                                                                                                    tGTATTTGGCTTTTTACTTTCGTGGATGATGAATGCAGTCAATTTATCCGGGCTACATGCGGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTGTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTg                 >  2:3598008/1‑140 (MQ=255)

                                                                                                                    tGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCCAGTTCTCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTg                 <  1:2297523/140‑1 (MQ=255)

                                                                                                                    tGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGAc                         <  2:4242347/132‑1 (MQ=255)

                                                                                                                    tGTATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTg                 >  1:862341/1‑140 (MQ=255)

                                                                                                                      tATTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGcc               <  2:2171255/140‑1 (MQ=255)

                                                                                                                       aTTTGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCg              <  2:689454/140‑1 (MQ=255)

                                                                                                                         ttGGCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCgtg            >  1:4104194/1‑140 (MQ=255)

                                                                                                                           ggCTTTTTACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTg                 >  2:2359057/1‑133 (MQ=255)

                                                                                                                                tttACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCa                                                                                          <  1:3169809/55‑1 (MQ=255)

                                                                                                                                tttACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCa                                                                                          >  2:3169809/1‑55 (MQ=255)

                                                                                                                                tttACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAatca     >  1:985421/1‑140 (MQ=255)

                                                                                                                                tttACTTTCGTGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAatca     >  1:3913122/1‑140 (MQ=255)

                                                                                                                                 ttccTTTCGTGGATGATGAATGCAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGAATGCCGTGTTAatcat    <  2:1292701/137‑1 (MQ=255)

CCGCAATATGGTAATCCCGTTTATTTAGGTATCTCCTTTGCCGTCTTAATTTTTATCTTGCTCATTACTCGCTATGCGAAAGGATTTATGTCCAACGTCGCCGTATTACTGGGGATTGTATTTGGCTTTTTACTTTCATGGATGATGAATGAAGTCAATTTATCCGGGCTACATGATGCTTCATGGTTTGCGATTGTTACGCCGATGTCGTTTGGTATGCCGATTTTCGATCCCGTTTCCATTCTGACCATGACTGCCGTGTTAATCATCG  >  NZ_CP025268/3034199‑3034469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`