BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,131,886	A→G	I200T (ATC→ACC)	yghO ←	protein YghO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,131,886	0	A	G	99.0%	1028.3 / NA	312	I200T (ATC→ACC)	yghO	protein YghO
Reads supporting (aligned to +/- strand): ref base A (0/1); major base G (118/189); minor base T (1/2); total (119/193)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 312 total aligned reads displayed.

AAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGATCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAATATGCGCGGCATACCAC  >  NZ_CP025268/3131750‑3132020

  gTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCAcc                                                                                                                                   <  1:4757332/140‑1 (MQ=255)

   ttGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCt                                                                                                                                  <  1:1600919/140‑1 (MQ=255)

     gATCGCCCATGGTCGCGAATTCATTTTCGTTAATGGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAGATCTCACGTAGGATGTGCATGTTGGTAGCGAACCGATGACGATTGATGCAGCGACTGGACAGCttt                                                                                                                                >  2:1904125/1‑140 (MQ=255)

     gATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCttt                                                                                                                                >  1:5156827/1‑140 (MQ=255)

     gATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCttt                                                                                                                                >  1:1938188/1‑140 (MQ=255)

      aTCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCtttt                                                                                                                               >  1:541574/1‑140 (MQ=255)

      aTCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCtttt                                                                                                                               >  1:2923280/1‑140 (MQ=255)

       tCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTTCCAGCCATAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCttttt                                                                                                                              >  1:2254127/1‑140 (MQ=255)

       tCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCttttt                                                                                                                              >  1:3912409/1‑140 (MQ=255)

        cGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTg                                                                                                                             <  2:2535464/140‑1 (MQ=255)

        cGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTg                                                                                                                             <  1:654209/140‑1 (MQ=255)

        cGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTg                                                                                                                             <  1:4457025/140‑1 (MQ=255)

         gCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTgc                                                                                                                            <  2:3317971/140‑1 (MQ=255)

          cccATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCACCAGTTGTGCTGCCAGACAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTgcg                                                                                                                           <  1:3744986/140‑1 (MQ=255)

             aTGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCAc                                                                                                                        >  1:2845778/1‑140 (MQ=255)

             aTGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCAc                                                                                                                        >  1:1982354/1‑140 (MQ=255)

                  cgcgAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGAt                                                                                                                   >  2:143818/1‑140 (MQ=255)

                    cgAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATcc                                                                                                                 >  2:3592047/1‑140 (MQ=255)

                                   tAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAgc                                                                                                  >  2:2890886/1‑140 (MQ=255)

                                     aaCGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAgcgc                                                                                                <  2:4451304/140‑1 (MQ=255)

                                     aaCGGCACAAATCCCCAGTTGTGCTACCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAgcgc                                                                                                <  2:3445285/140‑1 (MQ=255)

                                       cGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCg                                                                                              <  2:3242763/140‑1 (MQ=255)

                                           acaAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGgaga                                                                                          <  1:4410829/140‑1 (MQ=255)

                                           acaAATCCATCGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATATCTTCGGCAAACCGCTGACGATTGATGCAGCGAAGGGTCACCTTTTTGCGCACCTAATCCATCAGTTTTTTTAGCGCCGgaga                                                                                          <  2:3811893/140‑1 (MQ=255)

                                            caAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGgagag                                                                                         <  1:2729576/140‑1 (MQ=255)

                                               aTCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGaaa                                                                                      >  1:2443800/1‑140 (MQ=255)

                                                 ccctAGTTGTGCTGCCAGTCGGAGTTGAAGAGCTCACGCAGGATTTGCATCTCTTCGGCAAACCGCTGACGACTGATGCAGCGAATGGTCCCCTTTTTGCGCACATGTTCCATCAGTTTTTTTAGCGCCGGAGAGAAAGt                                                                                    <  2:868796/140‑1 (MQ=255)

                                                    cAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTgag                                                                                 >  1:2684682/1‑140 (MQ=255)

                                                      gTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGAt                                                                               <  2:2166707/140‑1 (MQ=255)

                                                      gTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGAt                                                                               <  1:1823693/140‑1 (MQ=255)

                                                          tgctgcCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGt                                                                           >  1:4023661/1‑140 (MQ=255)

                                                           gctgcCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGGGAAGGTGAGATCgggg                                                                          >  2:670157/1‑138 (MQ=255)

                                                            ctgcGAGCCCGAGTTCAAAATCTCACGGAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTc                                                                         >  2:4776652/1‑140 (MQ=255)

                                                             tgcCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCg                                                                        <  2:4546331/140‑1 (MQ=255)

                                                             tgcCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCg                                                                        <  2:2293928/140‑1 (MQ=255)

                                                               ccagccagAGTTGCAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCCGCGACTGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCt                                                                      <  2:1515709/140‑1 (MQ=255)

                                                               ccagccagAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTc                                                                         >  2:4065557/1‑137 (MQ=255)

                                                               ccagccagAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTc                                                                         >  2:2748611/1‑137 (MQ=255)

                                                               ccagccagAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCCGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCt                                                                      <  2:3159765/140‑1 (MQ=255)

                                                                 agccagAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGCGAAAGTGAGATCGGTTCGCTGc                                                                    >  2:2095165/1‑140 (MQ=255)

                                                                 agccagAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGc                                                                    >  2:5118495/1‑140 (MQ=255)

                                                                 agccagAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGc                                                                    >  2:1622555/1‑140 (MQ=255)

                                                                      gagTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATcca                                                               >  1:3876866/1‑140 (MQ=255)

                                                                       agTTGAAAATCTCACGCAGTATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATccac                                                              <  2:1825353/140‑1 (MQ=255)

                                                                       agTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATccac                                                              <  1:3221819/140‑1 (MQ=255)

                                                                         ttGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATccacca                                                            <  1:1486766/140‑1 (MQ=255)

                                                                         ttGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATccacca                                                            <  2:1336079/140‑1 (MQ=255)

                                                                            aaaaTCTCACGCAGGATCTGCATCGCTTCGGCAAACCGCGGACGATTGATGCAGCTAATGGTCACCTTGTTGTGCACCTGATCCATCAGTGTTTTTAGCGCCGGAGCGAAAGGGATCTCGGGTCGCTGCATCGACCACGc                                                         >  2:1280781/1‑140 (MQ=255)

                                                                                tctcACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGt                                                     <  1:1461030/140‑1 (MQ=255)

                                                                                tctcACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGt                                                     <  2:2478105/140‑1 (MQ=255)

                                                                                tctcACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGAGCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGAGCGGTTCGCTGCATCCACCACGCCAGt                                                     <  2:686739/140‑1 (MQ=255)

                                                                                 ctcACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTa                                                    >  1:4243404/1‑140 (MQ=255)

                                                                                   cACGCAGGATCTGCATCTCTTCGGCAAACCGCTGGCGATTGATGCAGCGAAGGGTCACCTTTTTGCGCACCTGATCCATCAGTTTCTTTCGCGCCGGAGAGAAAGTGAGATCGGTTCGCACCACCCACCACGCCaccaac                                                  >  2:490391/1‑135 (MQ=255)

                                                                                   cACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTaaa                                                  >  1:3814693/1‑140 (MQ=255)

                                                                                   cACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTaaa                                                  >  2:1053831/1‑140 (MQ=255)

                                                                                    aCGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAAt                                                 <  1:368404/140‑1 (MQ=255)

                                                                                    aCGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAAt                                                 <  1:3063197/140‑1 (MQ=255)

                                                                                       cAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCaa                                              <  2:3633175/140‑1 (MQ=255)

                                                                                        aGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAAt                                             <  1:968061/140‑1 (MQ=255)

                                                                                        aGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAAt                                             <  1:3966653/140‑1 (MQ=255)

                                                                                        aGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAAt                                             <  1:3162558/140‑1 (MQ=255)

                                                                                         ggATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATg                                            >  2:4690262/1‑140 (MQ=255)

                                                                                          gATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGc                                           >  2:5203652/1‑140 (MQ=255)

                                                                                            tCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCt                                         <  2:3060540/140‑1 (MQ=255)

                                                                                            tCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCt                                         <  1:1334986/140‑1 (MQ=255)

                                                                                             cTGCATCTCTTCTGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCAGGAGAGAAAGTGAGATCGGTTCGCTGCCTCCACCACGCCAGTAAATCAATGCCtt                                        <  2:4849398/140‑1 (MQ=255)

                                                                                             cTGCATCTCTTCGGCAAACCGCTGCCGCTCCATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCtt                                        <  2:4209345/140‑1 (MQ=255)

                                                                                                  tctcTACGGCCATCCGCCGTCGTCTGCTGCATCGGATGTTTATCTTTTTGCGCTCTTGGTCCATCAGTCTTTTTCGCGCCGGCGAGAATTTGCGATCGGTTCGCTGCATCCACCACGCCTGTAAATCAATGCCTTTGTGa                                   <  2:832750/140‑1 (MQ=255)

                                                                                                  tctcATCGCCAAACCGCTGACGATTGATGCAGCGCATGGTCACCGATTTGCGCCCCTGGTCCACCAGTTTTTTTAGCGCCGGACAGAAAGTGAGATCGGTTCGCGGCATCCACAACTCCAGTAAAGAAATGCCTTTATGa                                   <  2:4686708/140‑1 (MQ=255)

                                                                                                    tctTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATa                                 >  1:3545387/1‑140 (MQ=255)

                                                                                                    tctTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATa                                 >  2:5159492/1‑140 (MQ=255)

                                                                                                    tctTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATa                                 >  1:2794778/1‑140 (MQ=255)

                                                                                                    tctTCGGCAAACCGCTGACGATTGATGCAGCGAAGGGTCACCGTGTTGCGCACCTGATCCATCAGTTGTTTTAGCGCCGCAGAGAAAGTGAGACCGGTTCGGTGCATCCACCACGCCAGTAAACCAGTGCCATTGTGGTa                                 >  2:4247973/1‑140 (MQ=255)

                                                                                                     ctTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATaa                                <  1:3415667/140‑1 (MQ=255)

                                                                                                     ctTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATaa                                <  1:4507195/140‑1 (MQ=255)

                                                                                                     ctTCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATaa                                <  2:5090781/140‑1 (MQ=255)

                                                                                                      ttCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAAc                               >  2:1725235/1‑140 (MQ=255)

                                                                                                      ttCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAAc                               >  1:3474694/1‑140 (MQ=255)

                                                                                                       tCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAAcc                              <  1:2581474/140‑1 (MQ=255)

                                                                                                       tCGGCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAAcc                              >  1:4979882/1‑140 (MQ=255)

                                                                                                         ggCAAACCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCa                            <  1:4788193/140‑1 (MQ=255)

                                                                                                          gCAAACTGATCACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGCTCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCATTTGTGATAACCCAg                           <  1:1754474/140‑1 (MQ=255)

                                                                                                           aaaaCCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGGGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAgt                          <  2:1200219/139‑1 (MQ=255)

                                                                                                             aaCCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAgttg                        <  2:4350198/140‑1 (MQ=255)

                                                                                                              aCCGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAgttgt                       >  1:3300478/1‑140 (MQ=255)

                                                                                                               ccGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAgttgtt                      <  1:1005050/140‑1 (MQ=255)

                                                                                                                cGCTGACGATTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTc                     <  1:716606/140‑1 (MQ=255)

                                                                                                                        aTTGGTGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTCCAATATgcg             <  2:4603978/140‑1 (MQ=255)

                                                                                                                        aTTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAATATgcg             <  2:3865848/140‑1 (MQ=255)

                                                                                                                        aTTGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAATATgcg             <  1:3621108/140‑1 (MQ=255)

                                                                                                                        aTTGATGCAGCGAATGGTCACCTTTTAGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAATATgcg             <  2:2675712/140‑1 (MQ=255)

                                                                                                                        aTTGATGCAGCGAATGGTCACCGTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAATATgcg             <  2:3017120/140‑1 (MQ=255)

                                                                                                                         ttGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAATATgcgc            <  1:2464548/140‑1 (MQ=255)

                                                                                                                         ttGATGCAGCGAATGGACACCATTTGGCGCACCTGATCCATCAGCTTTTGGAGCGCCGGAGAGAGAGTGAGATCGGCTCGCTGCATCCACCACGCCAGTAAAGCAATGCCTTTGTGATAACCCAGTTGTTCAATATgctc            >  2:3713341/1‑138 (MQ=255)

                                                                                                                          tGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAATATGcgcg           <  1:2368075/140‑1 (MQ=255)

                                                                                                                          tGATGCAGCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAATATGcgcg           <  1:1854782/140‑1 (MQ=255)

                                                                                                                          agATGCAACGAATGATCCCATCTTTGCGCACCTCATCCATCAGTTTTTCTCGCGCAGGCGAGAAAGTGAGATCGGTTCGATGCATCCACAACCCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAATATGcgcg           <  2:3944914/139‑1 (MQ=255)

                                                                                                                                 gCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAATATGCGCGGCATAcc    <  1:4708418/140‑1 (MQ=255)

                                                                                                                                 gCGAATGGTCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGACAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAATATGCGCGGCATAcc    <  1:4651726/140‑1 (MQ=255)

                                                                                                                                 gCGAATGGTCACCTTGTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCACTGCCTTTGTGATAACCCAGTTGTTCAATATGCGCGGCATAcc    <  2:1988090/140‑1 (MQ=255)

AAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCATCTCTTCGGCAAACCGCTGACGATTGATGCAGCGAATGATCACCTTTTTGCGCACCTGATCCATCAGTTTTTTTAGCGCCGGAGAGAAAGTGAGATCGGTTCGCTGCATCCACCACGCCAGTAAATCAATGCCTTTGTGATAACCCAGTTGTTCAATATGCGCGGCATACCAC  >  NZ_CP025268/3131750‑3132020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`