BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,179,162	T→C	141 (TAA→TAG)	yqiB ←	DUF1249 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,179,162	0	T	C	99.3%	1010.9 / NA	304	141 (TAA→TAG)	yqiB	DUF1249 family protein
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (160/141); total (161/143)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 304 total aligned reads displayed.

TGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGAAGC  >  NZ_CP025268/3179026‑3179295

tGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCtaa                                                                                                                                    >  1:4221963/1‑140 (MQ=255)

 gTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGGAGGGTTAACAGGCTTTCCAAATGGTGGCCTTAGGTGTCACGACGCtaat                                                                                                                                   >  2:5048481/1‑140 (MQ=255)

 gTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCtaat                                                                                                                                   >  2:2329298/1‑140 (MQ=255)

   ttACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCtaataa                                                                                                                                 >  1:357628/1‑140 (MQ=255)

     aCCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAAc                                                                                                                               >  1:767187/1‑140 (MQ=255)

         cTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGa                                                                                                                           >  1:1077585/1‑140 (MQ=255)

                ggCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCa                                                                                                                    >  1:143761/1‑140 (MQ=255)

                ggCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCa                                                                                                                    >  1:1494362/1‑140 (MQ=255)

                ggCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCa                                                                                                                    <  1:3904528/140‑1 (MQ=255)

                ggCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCa                                                                                                                    >  1:1416041/1‑140 (MQ=255)

                ggCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCa                                                                                                                    >  1:2787556/1‑140 (MQ=255)

                 gCTTCGTGCTTTTGTGCAAACAGTTGAGTGTCGGTAATTTGTAAAATCCTGGCTCTGGCCTCACCAGCCAGACGAAGGGTTAACAGGCTTCCCTAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCAt                                                                                                                   >  1:3102429/1‑140 (MQ=255)

                 gCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCAt                                                                                                                   >  1:5020985/1‑140 (MQ=255)

                 gCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTGCACGACGCTAATAAACCGGAATCTCCAt                                                                                                                   >  2:2607172/1‑140 (MQ=255)

                 ccTTCGTGCTTTTGTGCAAACCGGTGAGTGTCGGTAATTTGAAAAATCCTGACTCTGGCCGCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGCGTCCTTAGGTTGCACGACGCTAATACACCGGAGTCGCCAt                                                                                                                   >  2:4311292/2‑140 (MQ=255)

                  cTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATc                                                                                                                  <  1:3538050/140‑1 (MQ=255)

                     cGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCt                                                                                                               >  1:1527284/1‑140 (MQ=255)

                     cGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCt                                                                                                               >  2:1875227/1‑140 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                             <  2:133082/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                             <  1:5069178/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                             <  1:2012431/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                             >  1:4501294/1‑140 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                             <  2:3963656/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                             <  2:4201748/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                             <  1:3426507/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCAAGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                             <  2:2759006/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGGAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                             <  2:4608257/140‑1 (MQ=255)

                       cgCTTTTGTGCAATCAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                             <  1:4579201/139‑1 (MQ=255)

                         cTTTTGTGCAAACAGGTGACTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCAt                                                                                                           <  2:934889/140‑1 (MQ=255)

                         cTTTCGTGCAAACAGGTGCGTGTCGGTAATTTGTAAGATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCAt                                                                                                           <  2:3400908/140‑1 (MQ=255)

                            ttGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCAtgtg                                                                                                        >  1:2671315/1‑140 (MQ=255)

                            ttGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCAtgtg                                                                                                        <  1:4679593/140‑1 (MQ=255)

                               tgCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCATCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTa                                                                                                     <  1:3716185/140‑1 (MQ=255)

                               tgCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTa                                                                                                     <  2:621830/140‑1 (MQ=255)

                               tgCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTa                                                                                                     <  1:725305/140‑1 (MQ=255)

                                 cAAACAGGTTAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTaaa                                                                                                   <  2:3247291/140‑1 (MQ=255)

                                 cAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTaaa                                                                                                   <  1:3935793/140‑1 (MQ=255)

                                 cAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTaaa                                                                                                   >  1:4009920/1‑140 (MQ=255)

                                 cAAACAGGTGAGTGTCGGTAATTTGTAAAATACTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTGCTTAGGTTTCACGACGCTAATAAACCGGAATCGTCATCGATCAATGTGCTaaa                                                                                                   >  1:4336046/1‑140 (MQ=255)

                                 cAAACAGGTGAGTGTCAGTAATTTGTAAACTCCTGACTCTGGCCTCAACAGACAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAATCCGGAATCGCCATCGCTCCATGTGCTaaa                                                                                                   <  2:4033860/140‑1 (MQ=255)

                                  aaaCAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAAc                                                                                                  <  1:2190908/140‑1 (MQ=255)

                                   aaCAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACa                                                                                                 <  1:862363/140‑1 (MQ=255)

                                    aCAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAg                                                                                                >  1:2639745/1‑140 (MQ=255)

                                        gTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCGTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCCTGTGCTAAACAGTATc                                                                                            >  1:2884944/1‑140 (MQ=255)

                                         tGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCg                                                                                           >  1:666621/1‑140 (MQ=255)

                                           aGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCa                                                                                         <  2:3996605/140‑1 (MQ=255)

                                             tgtCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTa                                                                                                     <  1:1753763/126‑1 (MQ=255)

                                                cGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAg                                                                                    >  2:3292954/1‑140 (MQ=255)

                                                 ggTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGt                                                                                   <  1:1735547/140‑1 (MQ=255)

                                                  gTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTc                                                                                  >  1:5151081/1‑140 (MQ=255)

                                                       ttGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTa                                                                             >  1:2494713/1‑140 (MQ=255)

                                                                    aCTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAAt                                                                >  2:889710/1‑140 (MQ=255)

                                                                     ctctGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAAtt                                                               <  1:1389213/140‑1 (MQ=255)

                                                                     ctctGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAAtt                                                               <  1:399220/140‑1 (MQ=255)

                                                                       ctGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTg                                                             <  1:2946439/140‑1 (MQ=255)

                                                                       ctGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTg                                                             <  1:321219/140‑1 (MQ=255)

                                                                         ggCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTaaa                                                                                                   <  2:4922614/100‑1 (MQ=255)

                                                                         ggCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGAt                                                           >  1:4685354/1‑140 (MQ=255)

                                                                          gCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATg                                                          >  1:3336152/1‑140 (MQ=255)

                                                                               aCCAGCCAGAGGGAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAACCTGATTAATTTGATGCtttt                                                     >  2:2852145/1‑140 (MQ=255)

                                                                               aCCAGCCAGAGGAAGGTTTAACAGGCTTTCCAAATGGGGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCACCCAGTCCGCTAAAAACTGATTAATTTGATGAtttt                                                     >  2:4835031/1‑140 (MQ=255)

                                                                               aCCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCtttt                                                     >  2:2696666/1‑140 (MQ=255)

                                                                                           aaGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGAt                                         >  1:4840504/1‑140 (MQ=255)

                                                                                            aGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCCCGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATg                                        <  2:1036526/140‑1 (MQ=255)

                                                                                            aGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATg                                        <  1:2735280/140‑1 (MQ=255)

                                                                                             gggTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGc                                       <  1:1631708/140‑1 (MQ=255)

                                                                                              ggTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                             <  1:1253633/69‑1 (MQ=255)

                                                                                              ggTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                             >  2:1253633/1‑69 (MQ=255)

                                                                                                ttAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAAc                                    <  2:76310/140‑1 (MQ=255)

                                                                                                  aaCAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACtt                                  >  1:924225/1‑140 (MQ=255)

                                                                                                  aaCAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACtt                                  >  1:580311/1‑140 (MQ=255)

                                                                                                      ggCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTtta                              <  1:4545149/140‑1 (MQ=255)

                                                                                                      ggCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTtta                              <  1:884991/140‑1 (MQ=255)

                                                                                                       gCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttat                             <  1:1964516/140‑1 (MQ=255)

                                                                                                        cTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttatt                            >  2:2660493/1‑140 (MQ=255)

                                                                                                        cTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttatt                            <  2:1401181/140‑1 (MQ=255)

                                                                                                         tttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttatta                           >  1:1817777/1‑140 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                          <  1:3265177/140‑1 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                          <  2:4396107/140‑1 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                          >  2:1151446/1‑140 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                          <  1:1016273/140‑1 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                          >  2:1745919/1‑140 (MQ=255)

                                                                                                          ttCAAAATGGTGTGCTTAGGTTTCACGACGCTAATAAGCCGGAATCGCCATCGCTCCATGTGCGAAACAGTATCGCAACCGGTCAGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                          <  2:1557657/140‑1 (MQ=255)

                                                                                                            cccaaTGGCGCCCTTAGGCTTCACGACGCTAATACACCGGAACCGCCATCGCTCCATGTGCTAAACACTATCGCAACCCGGCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGa                        <  2:1951438/137‑1 (MQ=255)

                                                                                                            ccAAATGGTGTCCTTAGGTTTCACGAGGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGa                        <  2:4088469/140‑1 (MQ=255)

                                                                                                            ccAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTGAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGa                        <  2:3102429/140‑1 (MQ=255)

                                                                                                            ccAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGa                        <  2:298227/140‑1 (MQ=255)

                                                                                                            ccAAATGGTCTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGa                        <  2:3578914/140‑1 (MQ=255)

                                                                                                               aaTGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATaa                     <  2:4733920/140‑1 (MQ=255)

                                                                                                                       ccTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACcg             >  2:2099001/1‑140 (MQ=255)

                                                                                                                          tAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGGTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCgcgc          >  2:4522938/1‑140 (MQ=255)

                                                                                                                               ttCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGa     <  1:4927574/140‑1 (MQ=255)

                                                                                                                                tCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCGGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATTATCATACCGCGCTTTGaa    >  2:3710047/1‑140 (MQ=255)

                                                                                                                                tCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGaa    >  2:2506458/1‑140 (MQ=255)

                                                                                                                                tCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGaa    >  2:442562/1‑140 (MQ=255)

                                                                                                                                tCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGaa    >  2:2197814/1‑140 (MQ=255)

                                                                                                                                 cACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGAAg   >  1:4074280/1‑140 (MQ=255)

                                                                                                                                  acgacgCTAATAAACCGGGATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGAGTAATTTGATGCTTTGCGTCGCGGGGGTGCAGGGTTTTGTTAGGATGAGCATACCGCGCTTTGaatc  >  2:2654182/1‑138 (MQ=255)

                                                                                                                                  acgacgCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTCAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGAAGc  >  2:2364170/1‑140 (MQ=255)

                                                                                                                                  acgacgCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGAAGc  >  2:824553/1‑140 (MQ=255)

TGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGAAGC  >  NZ_CP025268/3179026‑3179295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`