BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,258,360	A→G	G112G (GGT→GGC)	cyuP ←	amino acid permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,258,360	0	A	G	99.1%	769.5 / NA	232	G112G (GGT→GGC)	cyuP	amino acid permease
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (111/119); minor base T (2/0); total (113/119)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

TAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAA  >  NZ_CP025268/3258223‑3258494

tAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGcc                                                                                                                                      >  2:3003634/1‑140 (MQ=255)

tAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGcc                                                                                                                                      >  2:4896622/1‑140 (MQ=255)

tAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATGACGAAATAGAGCGCACCTAACAGGATGcc                                                                                                                                      >  2:4422983/1‑140 (MQ=255)

 aaaaGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGccc                                                                                                                                     <  2:1625039/140‑1 (MQ=255)

 aaaaGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGccc                                                                                                                                     <  1:4092548/140‑1 (MQ=255)

 aaaaGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTCGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGccc                                                                                                                                     <  2:4503656/140‑1 (MQ=255)

 aaaaGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGCTGGTGATGGCGGTGGAATAGACGAACATCGAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGccc                                                                                                                                     <  1:807900/140‑1 (MQ=255)

 aaaaGGGACTGACTGACAGCCACACTCCCGCCACGCCGAAGGTATGCAGGTAGGACGCACTATCGTTGGTGATGGCGGTGGCATAGACGACCATCCACATCCCCAGCATTACGACCTAGCGCGCCCCTAACAGGATGccc                                                                                                                                     <  2:3290934/140‑1 (MQ=255)

   aaGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCa                                                                                                                                   >  1:685657/1‑140 (MQ=255)

      ggACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGtt                                                                                                                                >  1:1144022/1‑140 (MQ=255)

      ggACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGtt                                                                                                                                <  1:3317121/140‑1 (MQ=255)

        ccTGTCTGACCGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGCTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTACCCGGATGCCCCAGtttt                                                                                                                              >  2:807900/2‑140 (MQ=255)

        aCTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGtttt                                                                                                                              >  2:960954/1‑140 (MQ=255)

        aCTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGtttt                                                                                                                              >  1:761736/1‑140 (MQ=255)

          tGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTa                                                                                                                            >  1:898126/1‑140 (MQ=255)

           gTCTGACAGCAACCCTTCCGTCACGTCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTAc                                                                                                                           >  1:4402961/1‑140 (MQ=255)

           gTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGATGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTAc                                                                                                                           <  2:4694239/140‑1 (MQ=255)

            tCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTAcc                                                                                                                          >  1:523523/1‑140 (MQ=255)

                 cAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAAt                                                                                                                     <  2:862352/140‑1 (MQ=255)

                  aGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATa                                                                                                                    <  2:1495292/140‑1 (MQ=255)

                    cAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAAc                                                                                                                  <  2:2170112/140‑1 (MQ=255)

                      aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGccc                                                                                                                                     <  1:971320/119‑1 (MQ=255)

                      aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCg                                                                                                                >  1:5073153/1‑140 (MQ=255)

                      aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCg                                                                                                                >  2:3375755/1‑140 (MQ=255)

                      aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCg                                                                                                                >  1:3455074/1‑140 (MQ=255)

                      aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGAATGCCCCAGTTTTTACCTAAATAACCg                                                                                                                >  2:2775093/1‑140 (MQ=255)

                      aCCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTACCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTATTTACCTAAATAACCg                                                                                                                >  1:2877778/1‑140 (MQ=255)

                            ccGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATg                                                                                                          >  1:4530256/1‑140 (MQ=255)

                             cGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGa                                                                                                         <  1:2535347/140‑1 (MQ=255)

                                 aCGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCt                                                                                                     <  2:4230796/140‑1 (MQ=255)

                                    ccGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCgg                                                                                                  >  2:3125246/1‑140 (MQ=255)

                                      gAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGt                                                                                                <  2:4593755/140‑1 (MQ=255)

                                      gAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGt                                                                                                <  2:3163958/140‑1 (MQ=255)

                                           tATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCt                                                                                           <  1:1840644/140‑1 (MQ=255)

                                             tGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCttt                                                                                         <  1:2972952/140‑1 (MQ=255)

                                                    aGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCt                                                                                  >  1:2034379/1‑140 (MQ=255)

                                                     ggAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAAt                                                                                                                     >  1:3587152/1‑104 (MQ=255)

                                                     ggAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTg                                                                                 <  2:5073153/140‑1 (MQ=255)

                                                     ggAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTg                                                                                 >  1:1664018/1‑140 (MQ=255)

                                                     ggAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTg                                                                                 >  2:1245590/1‑140 (MQ=255)

                                                     ggAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTg                                                                                 >  1:4061977/1‑140 (MQ=255)

                                                         gCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGa                                                                             <  1:3057682/140‑1 (MQ=255)

                                                          cACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGAt                                                                            >  2:5193340/1‑140 (MQ=255)

                                                            cTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTc                                                                          >  1:3139030/1‑140 (MQ=255)

                                                            cTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTc                                                                          >  1:1256167/1‑140 (MQ=255)

                                                             tATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCt                                                                         >  2:1685604/1‑140 (MQ=255)

                                                               tCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGc                                                                       <  1:4885113/140‑1 (MQ=255)

                                                                 gTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCa                                                                     <  1:2305425/140‑1 (MQ=255)

                                                                    ggTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTACCAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCg                                                                  >  2:62181/1‑140 (MQ=255)

                                                                    ggTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCg                                                                  <  2:3768680/140‑1 (MQ=255)

                                                                    ggTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCg                                                                  <  2:1535907/140‑1 (MQ=255)

                                                                    ggTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCg                                                                  <  1:1140386/140‑1 (MQ=255)

                                                                    ggTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCg                                                                  <  1:2422305/140‑1 (MQ=255)

                                                                    agtcaTGGCGGCAGCATAGACCAACATCCCAACCCCCCGCATTACGAAATAGAGTGCACCTAACAGGATGCCCCAGTTTTTACCTAAATACCCGCTAATGACGCCCGGGTACTCTTCACCTTCTGGTGATTCTGCAAGCg                                                                  <  2:1727642/136‑1 (MQ=255)

                                                                         tGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATta                                                             >  1:5150865/1‑140 (MQ=255)

                                                                         tGGCGGTGGAATAGACGAACATCCAAAGCACCAGCATTACGAAATAGATCGCACCTAACAGGATGCCCCAGTTTTTACCTAAAGGACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCGGCGTATta                                                             >  2:3808199/1‑140 (MQ=255)

                                                                          ggCGGTGGAATAGACGAACATCCAAATCACCAGCATTAGGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATtaa                                                            >  2:1385501/1‑140 (MQ=255)

                                                                          ggCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATtaa                                                            >  1:368510/1‑140 (MQ=255)

                                                                          ggCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATtaa                                                            >  2:1385262/1‑140 (MQ=255)

                                                                             ggTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATtaataa                                                         >  1:3267664/1‑140 (MQ=255)

                                                                                ggAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACa                                                      <  2:535395/140‑1 (MQ=255)

                                                                                ggAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCATATTAATAAACa                                                      <  2:5150865/140‑1 (MQ=255)

                                                                                    tAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCg                                                  <  1:1378192/140‑1 (MQ=255)

                                                                                      gACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCt                                                <  1:4186354/140‑1 (MQ=255)

                                                                                      gACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCt                                                <  2:4150581/140‑1 (MQ=255)

                                                                                      gACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCt                                                <  1:2217582/140‑1 (MQ=255)

                                                                                      gACGAACATCCAAATAACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCt                                                <  1:2474172/140‑1 (MQ=255)

                                                                                          aaCATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGaaa                                            <  1:1207788/140‑1 (MQ=255)

                                                                                           aCATCCAAATCACCAGCATTACGAAATAGGGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATACCCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAAc                                           <  2:4797179/140‑1 (MQ=255)

                                                                                           aCATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAAc                                           <  1:1605369/140‑1 (MQ=255)

                                                                                           aCATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAAc                                           >  1:960268/1‑140 (MQ=255)

                                                                                           aCATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAAc                                           <  2:2537176/140‑1 (MQ=255)

                                                                                            cATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACa                                          >  2:2246753/1‑140 (MQ=255)

                                                                                               ccAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCTAGCGTATTAATAAACAATCGCTGAAACAGAt                                       <  2:3953584/140‑1 (MQ=255)

                                                                                                cAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATTCCCCGGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTTATTCTGCCAGCGTATTTATAAATAATCTCTGAAACAGATa                                      >  2:181499/1‑140 (MQ=255)

                                                                                                   aTCACCAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACAt                                   <  1:2304717/140‑1 (MQ=255)

                                                                                                       ccAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGcc                               >  1:1989084/1‑140 (MQ=255)

                                                                                                        cAGCATTACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCg                              >  1:678849/1‑140 (MQ=255)

                                                                                                             ttACGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTaa                         <  1:1087436/140‑1 (MQ=255)

                                                                                                               aCGAAATAGAGCGCACCTAACAGGATGCCTCAGTTTTTACCTAAATCACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAAcc                       <  2:2080526/140‑1 (MQ=255)

                                                                                                               aCGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAAcc                       <  2:2207995/140‑1 (MQ=255)

                                                                                                               aCGAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAAcc                       <  1:4835971/140‑1 (MQ=255)

                                                                                                                cGAAATAGAGCGCAGCTAACAGGATGGGCCAGTTTTTACGTAAATAACCACTAATGCGGCTCGGGTGATCTTTACATTCTGGTGATTCGGCCAGCGTATTAATCAACAATCGCTGAAACAGAGGGATTGCCAGGTAACCa                      >  2:3677334/1‑140 (MQ=255)

                                                                                                                 gAAATAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCaa                     <  1:648725/140‑1 (MQ=255)

                                                                                                                     tAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCAc                 >  2:3536957/1‑140 (MQ=255)

                                                                                                                     tAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCAc                 >  2:3455328/1‑140 (MQ=255)

                                                                                                                     tAGAGCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGATAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCAc                 >  1:4447134/1‑140 (MQ=255)

                                                                                                                        ggcgcACCTAACCGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAGTCTTTACATTCTGGTGATTCTGCCAGCTTATTAATAAACAATCGCTGAAACAGATACAATGTCGGGGACCCAATCACGGa              >  2:1238361/2‑140 (MQ=255)

                                                                                                                        agCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGa              >  1:3013653/1‑140 (MQ=255)

                                                                                                                        agCGCACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGa              >  2:243853/1‑140 (MQ=255)

                                                                                                                        agCGCACCTAACAGGATGCCCCAGCTTTTACCTAAATAACCGCTAACGACGCTCGGGTAATCTTTACATTCTGGTCATTCTGCCCGCGTATTAATAAACAATCCCTGAAACAGATACATTGCCGGCCAACCCATCACCGa              >  2:759900/1‑140 (MQ=255)

                                                                                                                         gcgcACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGAt             <  2:2284771/140‑1 (MQ=255)

                                                                                                                          cgcACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATg            >  1:1462160/1‑140 (MQ=255)

                                                                                                                           gcACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATga           >  1:4965500/1‑140 (MQ=255)

                                                                                                                            cACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATgag          <  1:4250339/140‑1 (MQ=255)

                                                                                                                            cACCTAACAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATgag          <  1:3517884/140‑1 (MQ=255)

                                                                                                                                 aaCAGGATGCCCCAGTTTGTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCGGAAACAGATACATTGCCGGGTAACCAATCCCCGATGAGAGCaa     >  2:1537884/1‑140 (MQ=255)

                                                                                                                                   cAGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCaaaa   <  2:2492733/140‑1 (MQ=255)

                                                                                                                                    aGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCaaaaa  <  1:1610231/140‑1 (MQ=255)

                                                                                                                                    aGGATGCCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCaaaaa  <  1:2763191/140‑1 (MQ=255)

TAAAAGGGACTGTCTGACAGCAACCCTTCCGTCACGCCGAAGGTATGCAGGTAGGAAGCACTATCGTTGGTGATGGCGGTGGAATAGACGAACATCCAAATCACCAGCATTACGAAATAGAGCGCACCTAACAGGATACCCCAGTTTTTACCTAAATAACCGCTAATGACGCTCGGGTAATCTTTACATTCTGGTGATTCTGCCAGCGTATTAATAAACAATCGCTGAAACAGATACATTGCCGGGTAACCAATCACCGATGAGAGCAAAAA  >  NZ_CP025268/3258223‑3258494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`