BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	339,140	A→G	99 (TAA→TAG)	CXP41_RS01780 →	protein YahH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	339,140	0	A	G	99.0%	1333.7 / NA	415	99 (TAA→TAG)	CXP41_RS01780	protein YahH
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (167/244); minor base T (3/1); total (170/245)
Fisher's exact test for biased strand distribution p-value = 3.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 415 total aligned reads displayed.

GGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCCAG  >  NZ_CP025268/339003‑339274

ggcgTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCTAGAGGTGTATGc                                                                                                                                      >  2:2940008/1‑140 (MQ=14)

ggcgTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCATACGAGTGGCGCGAGAATTGTAGGc                                                                                                                                      <  2:1739842/140‑1 (MQ=255)

     ggACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGTCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGAt                                                                                                                                 <  1:695030/140‑1 (MQ=255)

      gACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATa                                                                                                                                <  2:651309/140‑1 (MQ=255)

          ccTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCg                                                                                                                            <  1:3783341/140‑1 (MQ=255)

            ttATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTa                                                                                                                          <  1:175037/140‑1 (MQ=255)

            ttATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTa                                                                                                                          <  1:2214585/140‑1 (MQ=255)

            ttATCCGGCCTACGAGTGGCGAGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCCTTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGAGAGAATTGTAGGCCTGATAAGCGTa                                                                                                                          <  2:4186663/140‑1 (MQ=255)

              aTCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAgc                                                                                                                        <  2:2584072/140‑1 (MQ=255)

                   gCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATc                                                                                                                   >  1:1263983/1‑140 (MQ=255)

                   gCCGACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCAGCAGGCATTTGTCACCTTTGCCGGATGCGGCGGGAACGCCGTAGCCGGCCTACGGGTGGCGCGAGAATGGTGGGCCTGGTAAGCGTTGCGCagt                                                                                                                   >  2:1629499/1‑138 (MQ=14)

                    ccTACGAGTGGCGCGAGAATCTGTATGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGCCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCa                                                                                                                  >  2:4668046/1‑140 (MQ=255)

                    ccTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCa                                                                                                                  >  1:142616/1‑140 (MQ=255)

                     cTACGCATGGCCCAAGACTCTGTAGGCCTGAGAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGGGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAg                                                                                                                 <  2:918612/140‑1 (MQ=11)

                     cTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAg                                                                                                                 >  2:3320928/1‑140 (MQ=255)

                     cTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAg                                                                                                                 <  1:4854490/140‑1 (MQ=255)

                     cTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAGTGTAGGCCTGATAAGCGTAGCGCATCAg                                                                                                                 >  1:4915357/1‑140 (MQ=255)

                      tACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACTATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGCAGCGCATCAgg                                                                                                                <  1:4309830/140‑1 (MQ=255)

                      tACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                                                                <  2:1521668/140‑1 (MQ=255)

                      tACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                                                                <  1:1049431/140‑1 (MQ=255)

                      tACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                                                                <  1:84473/140‑1 (MQ=255)

                      tACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                                                                <  1:5059085/140‑1 (MQ=255)

                      tACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                                                                <  1:2649122/140‑1 (MQ=255)

                      tACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                                                                <  1:2377551/140‑1 (MQ=255)

                      tACGACTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                                                                <  2:4578241/140‑1 (MQ=255)

                      aaCGGGTGGCGCGGCAATCTGTAGCCCTGATAACCGTAGCGCATCAGGCATTTGTCACCATTGCCGGAGGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                                                                <  2:4932844/139‑1 (MQ=14)

                        cGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                                                                              >  1:3052703/1‑140 (MQ=255)

                          aGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGt                                                                                                            <  1:3598465/140‑1 (MQ=255)

                          aGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATGTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGt                                                                                                            <  1:617745/140‑1 (MQ=255)

                           gTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTc                                                                                                           <  2:5069711/140‑1 (MQ=255)

                             ggCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTg                                                                                                         >  1:335883/1‑140 (MQ=255)

                              gcgcGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTgg                                                                                                        >  1:1698751/1‑140 (MQ=255)

                              gcgcGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGAGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTgg                                                                                                        >  2:103232/1‑140 (MQ=255)

                                gcgAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCg                                                                                                      <  1:1112801/140‑1 (MQ=255)

                                gcgAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCg                                                                                                      <  1:4444384/140‑1 (MQ=255)

                                gcgAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCg                                                                                                      <  2:3286739/140‑1 (MQ=255)

                                 cgAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGt                                                                                                     <  1:4168440/140‑1 (MQ=255)

                                 cgAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGt                                                                                                     <  1:2740849/140‑1 (MQ=255)

                                 cgAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGt                                                                                                     <  2:3064161/140‑1 (MQ=255)

                                  gagaATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGtt                                                                                                    <  2:4841280/140‑1 (MQ=255)

                                        cTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCAt                                                                                              <  1:3199316/140‑1 (MQ=255)

                                        cTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCAt                                                                                              <  2:4397586/140‑1 (MQ=255)

                                         tGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAAGAGGGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATa                                                                                             <  1:340192/140‑1 (MQ=255)

                                              gCCTGATAACCGTAGCGACTCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCATACGCGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAAcccc                                                                                        <  2:3977383/140‑1 (MQ=17)

                                               ccTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCa                                                                                       <  2:2739268/140‑1 (MQ=255)

                                               ccTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCa                                                                                       <  1:1902413/140‑1 (MQ=255)

                                               ccTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCa                                                                                       <  2:2091867/140‑1 (MQ=255)

                                               ccTGATAAGAGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCa                                                                                       <  1:3974925/140‑1 (MQ=255)

                                               catgctAAGCGTAGCGCATCAGGCATTTGTCAACATTGCCGGATGCGGCGTGACCGCATTATCAGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCa                                                                                       <  2:2820901/135‑1 (MQ=17)

                                                 tGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATc                                                                                     <  1:2585410/140‑1 (MQ=255)

                                                   aTACGCGTAGCGCATCAGGCATTTATCAATATTGCCGGATGCGGCGTGCACGGCTTATACGGTCTACGAGTCGCGCGACACTTGTAGGCCTGATAATCGTCGCGCATCAGGCAGTCTGGCGTTGGTCATAAACCAATCAc                                                                                   <  2:2337300/140‑1 (MQ=17)

                                                   aTAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCAc                                                                                   >  2:538542/1‑140 (MQ=255)

                                                   aTAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTCGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCCTCAc                                                                                   >  2:692153/1‑140 (MQ=255)

                                                    tAAGCGTAGCGCATCCGGCATTTGTCACCATCAGCGGAGGCGGCGTGACCGCCTTGTCCGGCCTACGAGTGGCGCGAGCATTGTAGGCCTGATAGGCGTAGCGCGTCAGGCGATGTGGCGTGGGCCACAAACCCATcccc                                                                                  <  2:408411/140‑1 (MQ=9)

                                                    tAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCAcc                                                                                  <  2:3187613/140‑1 (MQ=255)

                                                    tAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCAcc                                                                                  <  2:1110084/140‑1 (MQ=255)

                                                    tAAGCGTAGCGCATCAGGCATTTGCCACCACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCAcc                                                                                  <  2:2904299/140‑1 (MQ=255)

                                                      aGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGACCGCCTTAACCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTCGCGCATCAGGCACTCTGGCGTTGGTCATAACCCCATCACCct                                                                                <  2:244971/140‑1 (MQ=18)

                                                      aGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCct                                                                                <  1:1334749/140‑1 (MQ=255)

                                                        cGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGGGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCctct                                                                              >  2:1373613/1‑140 (MQ=255)

                                                          tAGCGCATCAGGCATTTGTCACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGt                                                                            >  1:1818519/1‑140 (MQ=255)

                                                             cgcATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTGTGGCGCGAGAGGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGc                                                                         >  2:3725943/1‑140 (MQ=18)

                                                             cgcATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGc                                                                         >  1:1651952/1‑140 (MQ=255)

                                                               cATCAGGCCTTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCgg                                                                       <  2:2771874/140‑1 (MQ=255)

                                                               cATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCgg                                                                       <  1:1483225/140‑1 (MQ=255)

                                                                aTCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAGTGTAGGCCCGATAAGCGTAGCGCATCAGGCAGGCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGa                                                                      <  1:3231879/140‑1 (MQ=255)

                                                                  cAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACa                                                                    >  2:262389/1‑140 (MQ=255)

                                                                     gCATTTGTCACCATTGCCGGATGCGGCGTGACCGCCTTATCCGGCCGACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                                                                 <  1:3096619/140‑1 (MQ=255)

                                                                     gCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaa                                                                 <  1:3907871/140‑1 (MQ=255)

                                                                        tttGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCCCCCTCTGTAGCGGACATaacaa                                                              <  1:4987306/140‑1 (MQ=255)

                                                                         ttGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaacaac                                                             >  2:3488144/1‑140 (MQ=255)

                                                                          tGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGTAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAacaaca                                                            >  2:4113256/1‑140 (MQ=255)

                                                                          tGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAacaaca                                                            >  2:1315502/1‑140 (MQ=255)

                                                                          tGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATGGTAGGCCTGATAAGCGGAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAacaaca                                                            >  2:1937527/1‑140 (MQ=255)

                                                                           ttCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACAt                                                           <  2:3460678/139‑1 (MQ=255)

                                                                                   tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCGGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGc                                                   >  1:2821355/1‑140 (MQ=255)

                                                                                   tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAAAAACATGAAAGAGc                                                   >  2:4468072/1‑140 (MQ=255)

                                                                                     ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCtt                                                 >  1:2968908/1‑140 (MQ=255)

                                                                                        gATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTtgtg                                              >  1:1958828/1‑140 (MQ=255)

                                                                                          tGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGt                                            >  2:73195/1‑140 (MQ=255)

                                                                                                       ccTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATtggt                               <  2:2161106/140‑1 (MQ=255)

                                                                                                       ccTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATtggt                               <  2:3768845/140‑1 (MQ=255)

                                                                                                        cTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATtggtg                              >  1:2593207/1‑140 (MQ=255)

                                                                                                            tCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCaa                          <  2:117984/140‑1 (MQ=255)

                                                                                                            tCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCGTGTGGTCGTTGCCATTGGTGGCaa                          <  1:3522977/140‑1 (MQ=255)

                                                                                                                 ccgaCGGGTGGCGCGAGAATTGTAGGCCCGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACGCCAGAACGCTCTGTGGCGGACGTAACAACGTGAAGGAGCGTGTGCGCGTTGCCATGGGTGGCAACAgga                     <  2:5177797/137‑3 (MQ=25)

                                                                                                                 ccTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa                     <  1:3742452/140‑1 (MQ=255)

                                                                                                                 ccTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa                     <  1:4039509/140‑1 (MQ=255)

                                                                                                                 ccTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa                     <  1:2910902/140‑1 (MQ=255)

                                                                                                                 ccTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCa                     <  2:1446330/140‑1 (MQ=255)

                                                                                                                  cTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCAt                    >  1:4752424/1‑140 (MQ=255)

                                                                                                                  cTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCAt                    >  1:4494165/1‑140 (MQ=255)

                                                                                                                         tGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCaaa             >  1:928305/1‑140 (MQ=255)

                                                                                                                           gcgcGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTa                                                                           >  1:5181495/1‑76 (MQ=255)

                                                                                                                           gcgcGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGa           >  2:2979873/1‑140 (MQ=255)

                                                                                                                           gcgcGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGa           >  1:4642773/1‑140 (MQ=255)

                                                                                                                           gcgcGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGa           >  1:1191309/1‑140 (MQ=255)

                                                                                                                           gcgcGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGa           >  2:3605331/1‑140 (MQ=255)

                                                                                                                           ccgcgAGAATTGTAGGCCCGATAAGCGGAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGACAGAGCTGGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGa           <  2:1994762/139‑1 (MQ=255)

                                                                                                                                agaATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAACGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACg      <  2:3533223/140‑1 (MQ=255)

GGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAAGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCCAG  >  NZ_CP025268/339003‑339274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`