BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,386,542	T→C	91 (TAA→TAG)	yhcO ←	barstar family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,386,542	0	T	C	99.4%	566.6 / NA	170	91 (TAA→TAG)	yhcO	barstar family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (110/58); minor base A (1/0); total (112/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

AAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGG  >  NZ_CP025268/3386410‑3386673

 aGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATg                                                                                                                                 >  2:2852130/1‑137 (MQ=255)

 aGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACg                                                                                                                              <  1:4571269/140‑1 (MQ=255)

 aGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACg                                                                                                                              <  1:1749510/140‑1 (MQ=255)

   gcgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGaa                                                                                                                            <  1:59707/140‑1 (MQ=255)

   gcgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGaa                                                                                                                            <  2:1659554/140‑1 (MQ=255)

       aaCGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACAtt                                                                                                                        >  1:3899893/1‑140 (MQ=255)

       aaCGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACAtt                                                                                                                        >  2:2023247/1‑140 (MQ=255)

              tACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGc                                                                                                                 >  1:2627618/1‑140 (MQ=255)

               aCCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCa                                                                                                                >  1:2573917/1‑140 (MQ=255)

                  aGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAAt                                                                                                             <  1:1089517/140‑1 (MQ=255)

                   gATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATg                                                                                                            <  1:231407/140‑1 (MQ=255)

                     ttACTGAAGCTCGTAGCGGTGACACCTGGCACTCTACGGCTGAACTGTACAAATAAACCCTGAGCGTCTTGTCCGACGATATTTCCCTCGGTTCGGGGTCTTTTTTTTGGGGTAAGGACGAACATTAAAACGCAAATGcc                                                                                                          >  2:1741217/1‑140 (MQ=255)

                     ttACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACACAAATGcc                                                                                                          >  1:3874159/1‑140 (MQ=255)

                      tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTCATGACGAACATTAAACAGCAAATGccc                                                                                                         >  2:961327/1‑140 (MQ=255)

                      tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGccc                                                                                                         >  1:1803824/1‑140 (MQ=255)

                         tGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTc                                                                                                      <  2:1717909/140‑1 (MQ=255)

                          gAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                                                     <  1:1693246/140‑1 (MQ=255)

                          gAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                                                     >  2:1663581/1‑140 (MQ=255)

                          gAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                                                     <  1:3001169/140‑1 (MQ=255)

                          gAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                                                     <  1:763088/140‑1 (MQ=255)

                           aaGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGACCTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACAGTAAAACGCAAATGCCCTTCCa                                                                                                    >  1:537152/1‑140 (MQ=255)

                           aaGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCa                                                                                                    >  2:92033/1‑140 (MQ=255)

                             gCTCGTAGCGGTGACACCTGGCACGCTACGGATGAAATGTACAAATGAACCCACATCGTCTTGTCCGACGCTATTGCCCCTGGTTCGGGAGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAACTGACATTcacgc                                                                                                  >  2:681022/1‑136 (MQ=255)

                              cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                                 >  2:3233515/1‑140 (MQ=255)

                              cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                                 >  1:112850/1‑140 (MQ=255)

                              cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                                 >  1:812543/1‑140 (MQ=255)

                              cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                                 >  2:3737106/1‑140 (MQ=255)

                              cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGcc                                                                                                 >  2:3233862/1‑139 (MQ=255)

                               tCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGctc                                                                                                <  1:4905014/140‑1 (MQ=255)

                                cGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGTCGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGctct                                                                                               >  2:4578579/1‑140 (MQ=255)

                                cGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGctct                                                                                               >  2:4639425/1‑140 (MQ=255)

                                  tAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                                 >  1:3242128/1‑136 (MQ=255)

                                  tAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTc                                                                                             >  1:719874/1‑140 (MQ=255)

                                  tAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTc                                                                                             >  1:2344076/1‑140 (MQ=255)

                                       gTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                                                        >  1:1040367/1‑140 (MQ=255)

                                       gTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                                                        <  1:1302282/140‑1 (MQ=255)

                                        tGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGc                                                                                       >  2:832808/1‑140 (MQ=255)

                                          acacCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCt                                                                                     <  1:3978730/140‑1 (MQ=255)

                                          acacCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCt                                                                                     >  2:5003434/1‑140 (MQ=255)

                                               tGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatca                                                                                <  1:3567797/140‑1 (MQ=255)

                                               tGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatca                                                                                >  2:3867067/1‑140 (MQ=255)

                                               tGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtcatca                                                                                <  1:3567078/140‑1 (MQ=255)

                                                ggCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaa                                                                               >  1:2318551/1‑140 (MQ=255)

                                                  cACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAc                                                                             >  2:499559/1‑140 (MQ=255)

                                                     gCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGc                                                                         <  1:450277/140‑1 (MQ=255)

                                                     gCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGc                                                                         <  1:450419/140‑1 (MQ=255)

                                                     gCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGc                                                                         <  1:4934896/140‑1 (MQ=255)

                                                      cTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCGCTTCCTCTGCCTCGTCAAAC‑AGCa                                                                        >  2:5097587/1‑140 (MQ=255)

                                                       tACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCaa                                                                       >  2:1042081/1‑140 (MQ=255)

                                                            cTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTa                                                                  >  1:858883/1‑140 (MQ=255)

                                                             tGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTaa                                                                 <  2:148018/140‑1 (MQ=255)

                                                              gAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAAc                                                                <  2:2989547/140‑1 (MQ=255)

                                                               aaCTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAAcg                                                               >  1:4935647/1‑140 (MQ=255)

                                                               aaCTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAAcg                                                               >  2:3447577/1‑140 (MQ=255)

                                                                 cTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAAcgcg                                                             >  2:1534908/1‑140 (MQ=255)

                                                                   gTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGcc                                                           >  2:3125352/1‑140 (MQ=255)

                                                                    tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGTTTCGGGGGCTTTTTTTTGCGCTAATGACGCACCTTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCa                                                          >  2:4269368/1‑140 (MQ=255)

                                                                    tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCa                                                          >  1:1728050/1‑140 (MQ=255)

                                                                    tACAAATAAACCCTCATCGCCTTGTCCGACGACGTTGCCACCGGCTCGGGGGCCTTTTTTTGCGCTAGTGACGAACATAAAAACCAACATGCCCATCCAGCCCTTCCCCTGCCCCA‑CACACAAGCAGAATGAACGCGCCa                                                          >  2:3591163/1‑140 (MQ=255)

                                                                      cAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCaaa                                                        >  2:1298108/1‑140 (MQ=255)

                                                                      cAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCaaa                                                        >  2:5049113/1‑140 (MQ=255)

                                                                         aTAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAAcc                                                     >  1:1227488/1‑139 (MQ=255)

                                                                         aTAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACg                                                     >  1:2843328/1‑140 (MQ=255)

                                                                         aTAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACg                                                     >  1:1646559/1‑140 (MQ=255)

                                                                         aTAAACCCTCATCGTCTTGTCCGACGAGATTGCCCCCGGGTCGGGGGCTTTTTTTTGCGCTAATGACGAGCATTAAAACGCAAATGCGCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACg                                                     >  2:1579870/1‑140 (MQ=255)

                                                                              cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGAc                                                >  1:2125954/1‑140 (MQ=255)

                                                                              cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGAc                                                >  1:4211707/1‑140 (MQ=255)

                                                                              cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGAc                                                >  1:2526835/1‑140 (MQ=255)

                                                                              cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGAc                                                >  1:1238578/1‑140 (MQ=255)

                                                                              cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGAc                                                >  1:2525799/1‑140 (MQ=255)

                                                                                 tCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGAcgcg                                             <  1:1804744/140‑1 (MQ=255)

                                                                                 tCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCCCCTCAAAC‑AGCAATATTAACGCGCCAAAACGGCGAcgcg                                             >  1:3369017/1‑140 (MQ=255)

                                                                                 tCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAAAATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAACACGGCGAagct                                             >  1:3041206/1‑139 (MQ=255)

                                                                                   aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGtt                                           >  2:4266305/1‑140 (MQ=255)

                                                                                   aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGtt                                           >  1:4693244/1‑140 (MQ=255)

                                                                                     cGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGtttt                                         <  2:1295670/140‑1 (MQ=255)

                                                                                       tCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGTTTtct                                       >  1:887639/1‑140 (MQ=255)

                                                                                           gTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGc                                                                                                  >  2:2414821/1‑78 (MQ=255)

                                                                                           gTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGc                                                                                                  <  1:2418010/78‑1 (MQ=255)

                                                                                           gTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGc                                                                                                  >  2:2418010/1‑78 (MQ=255)

                                                                                           gTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTcc                                   >  2:3919229/1‑140 (MQ=255)

                                                                                              cgacgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAg                                <  2:5082934/140‑1 (MQ=255)

                                                                                              cgacgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAg                                <  2:4187883/140‑1 (MQ=255)

                                                                                               gacgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGAAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGa                               <  1:349303/140‑1 (MQ=255)

                                                                                               gacgaTATTGCCCCCGGTCCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTGTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGTTCTCTCTCCCAGa                               <  2:3704732/140‑1 (MQ=255)

                                                                                               gacgaTATTGCCCCCGGCTCCGGGGCTTTTTTTCGCCATAATAACGCGCCTTAAAACGAAACTGGCCTTCCAGCTCTTCCTCTGCGTCATCCAAC‑AGCAATATCAACGCGCCAAAACGGCGACGCGTCTTCTCTGCCAGa                               >  2:2651106/1‑140 (MQ=255)

                                                                                                 cgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGATGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATg                             >  1:1548564/1‑140 (MQ=255)

                                                                                                 cgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATg                             >  1:2912917/1‑140 (MQ=255)

                                                                                                 cgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACAGTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATg                             >  2:4376071/1‑140 (MQ=255)

                                                                                                      ttGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACaa                        >  1:4724200/1‑140 (MQ=255)

                                                                                                             cGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCGCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAt                 <  2:2927803/140‑1 (MQ=255)

                                                                                                              ggTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAtc                >  1:380660/1‑140 (MQ=255)

                                                                                                              ggTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAtc                >  2:829516/1‑140 (MQ=255)

                                                                                                              ggTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAtc                >  1:292360/1‑140 (MQ=255)

                                                                                                              ggTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAtc                >  2:5192749/1‑140 (MQ=255)

                                                                                                                       gCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGt       <  1:1021114/140‑1 (MQ=255)

                                                                                                                       gCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGt       <  1:1020871/140‑1 (MQ=255)

                                                                                                                         ttttttttGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAg     <  2:3563598/140‑1 (MQ=255)

                                                                                                                            tttttGCGCTAATGACTAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAACC‑AGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCCGATGAACAAATTCAATCTCAAGTGGTAGCgg  <  1:2887129/140‑1 (MQ=255)

                                                                                                                            tttttGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCgg  <  2:4935647/140‑1 (MQ=255)

AAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAAC‑AGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGG  >  NZ_CP025268/3386410‑3386673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`