BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,535,342	T→C	R405G (AGG→GGG)	envZ ←	two‑component system sensor histidine kinase EnvZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,535,342	0	T	C	100.0%	901.3 / NA	269	R405G (AGG→GGG)	envZ	two‑component system sensor histidine kinase EnvZ
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (161/108); total (161/108)

Only 100 of 269 total aligned reads displayed.

CCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCTTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTG  >  NZ_CP025268/3535206‑3535478

   tcttTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATcc                                                                                                                                    >  2:1395517/1‑140 (MQ=255)

    cttTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATccc                                                                                                                                   >  1:3834094/1‑140 (MQ=255)

    cttTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATccc                                                                                                                                   <  1:2479234/140‑1 (MQ=255)

      tttGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGt                                                                                                                                 <  2:3174764/140‑1 (MQ=255)

      tttGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCGCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGt                                                                                                                                 <  2:3704707/140‑1 (MQ=255)

            gTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCt                                                                                                                           >  2:3865242/1‑140 (MQ=255)

            gTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCt                                                                                                                           >  1:961292/1‑140 (MQ=255)

               cccTGCGCCCGCGTTACCGGCCCTGGCAGCCAGGCGCGAATGGACAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCCCAATTGCCAGCCCTAATCCCGTGCCGCTAAt                                                                                                                        <  1:1376529/140‑1 (MQ=255)

               cccTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAAt                                                                                                                        <  2:4241050/140‑1 (MQ=255)

               cccTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAACGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAAt                                                                                                                        <  1:4779714/140‑1 (MQ=255)

               cccTGCGCCCGCGGTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAAt                                                                                                                        <  1:1321133/140‑1 (MQ=255)

                    cgcCCGCGGTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCCAGCTCCAGCATCCCGTGATGGTTATCCACGATACGCTGCACAAGTGCCAGCCCTAATCCCGTGCCGCTCATGGGgc                                                                                                                   >  2:3501040/1‑140 (MQ=255)

                     gcCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTgcg                                                                                                                  <  2:4498073/140‑1 (MQ=255)

                      cccGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTgcgc                                                                                                                 >  2:3539169/1‑140 (MQ=255)

                       ccGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTgcgcg                                                                                                                <  2:4771207/140‑1 (MQ=255)

                       ccGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGACCTAATCCCGTGCCGCTAATGGTgcgcg                                                                                                                <  1:4198301/140‑1 (MQ=255)

                         gcgTTACCGGCACTGGCAGCCAGGCGCCAATGGAAATCCCGCCCCGGTCGCTGGTGCCAAGCTGCAGCAGCCCGTGATGGTTATCCACTGTACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCa                                                                                                              >  2:2497953/1‑140 (MQ=255)

                          cgTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCAc                                                                                                             >  2:4003147/1‑140 (MQ=255)

                              aCCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTc                                                                                                         >  2:691105/1‑140 (MQ=255)

                              aCCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTc                                                                                                         >  1:4138742/1‑140 (MQ=255)

                                 ggCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTcgcc                                                                                                      >  1:356781/1‑140 (MQ=255)

                                    aCTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCcgcg                                                                                                   <  1:796931/140‑1 (MQ=255)

                                     cTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCgg                                                                                                  >  1:3058578/1‑140 (MQ=255)

                                         cAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACaa                                                                                              >  1:4470477/1‑140 (MQ=255)

                                         cAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACaa                                                                                              >  1:4283814/1‑140 (MQ=255)

                                         cAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACaa                                                                                              <  1:3493271/140‑1 (MQ=255)

                                           gCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAAc                                                                                            <  1:2958015/140‑1 (MQ=255)

                                            ccAGGCTCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAACGTCCAGCATCCCGTTAGGGTTATCCGCGATACGCTGCACAATATCCATCCCTAATTCCGTGCCTCTAATTGAGCGCAGACTGTCGCCGGGGGCAACCg                                                                                           >  2:2824005/1‑140 (MQ=255)

                                              aGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGc                                                                                         <  2:2236876/140‑1 (MQ=255)

                                              aGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGc                                                                                         <  1:2251261/140‑1 (MQ=255)

                                              aGGAGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGc                                                                                         <  2:2723354/140‑1 (MQ=255)

                                                gcgcGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTg                                                                                       >  1:1562192/1‑140 (MQ=255)

                                                   cgAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGaa                                                                                    <  2:5017272/140‑1 (MQ=255)

                                                    gAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAAc                                                                                   <  1:579480/140‑1 (MQ=255)

                                                     aaTGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACa                                                                                  <  2:2593459/140‑1 (MQ=255)

                                                         gAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGtt                                                                              >  1:4602233/1‑139 (MQ=255)

                                                          aaaGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGc                                                                             <  1:3202393/140‑1 (MQ=255)

                                                          aaaGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGc                                                                             <  2:2070220/140‑1 (MQ=255)

                                                          aaaGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGc                                                                             <  1:4658685/140‑1 (MQ=255)

                                                          aaaGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGc                                                                             <  2:2062329/140‑1 (MQ=255)

                                                           aaGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCt                                                                            <  1:3133938/140‑1 (MQ=255)

                                                             gcccgcccCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTa                                                                          <  1:5157023/140‑1 (MQ=255)

                                                                cgccccgcTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACgt                                                                       <  2:2495628/140‑1 (MQ=255)

                                                                cgccccgcTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACgt                                                                       >  2:5053758/1‑140 (MQ=255)

                                                                  ccccgcTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACgttg                                                                     >  1:3952382/1‑140 (MQ=255)

                                                                    ccgcTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACgttgtt                                                                   >  1:3160141/1‑140 (MQ=255)

                                                                       ctcgctGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCg                                                                >  1:2524739/1‑140 (MQ=255)

                                                                       ctcgctGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCg                                                                >  1:3475534/1‑140 (MQ=255)

                                                                           cttgTGACAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGgcgc                                                            <  2:2999187/137‑1 (MQ=255)

                                                                               tGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAAtt                                                        >  2:56195/1‑140 (MQ=255)

                                                                                 ccAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTcc                                                      <  2:1402786/140‑1 (MQ=255)

                                                                                 ccAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTcc                                                      >  1:20108/1‑140 (MQ=255)

                                                                                 ccAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTcc                                                      >  1:1536917/1‑140 (MQ=255)

                                                                                 ccAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTcc                                                      >  1:4810421/1‑140 (MQ=255)

                                                                                     gCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGa                                                  <  2:4995942/140‑1 (MQ=255)

                                                                                      cTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGAc                                                 >  2:4278704/1‑140 (MQ=255)

                                                                                      cTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGAc                                                 <  1:1805582/140‑1 (MQ=255)

                                                                                      cTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGAc                                                 >  2:26713/1‑140 (MQ=255)

                                                                                          aGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTc                                             <  2:1600709/140‑1 (MQ=255)

                                                                                          aGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAACTCCCGGACCTTc                                             >  2:2030599/1‑140 (MQ=255)

                                                                                               ccccTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTAGCCGTTGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCtt                                        <  2:436455/140‑1 (MQ=255)

                                                                                               cccGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCtt                                        <  2:3343703/140‑1 (MQ=255)

                                                                                               cccGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCtt                                        <  2:5166372/140‑1 (MQ=255)

                                                                                               cccGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCtt                                        <  1:1988494/140‑1 (MQ=255)

                                                                                                ccGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTc                                       >  1:1007064/1‑140 (MQ=255)

                                                                                                ccGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTc                                       >  1:842046/1‑140 (MQ=255)

                                                                                                ccGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTc                                       <  1:193764/140‑1 (MQ=255)

                                                                                                ccGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTc                                       >  2:4835145/1‑140 (MQ=255)

                                                                                                      tGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTTCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTAACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTg                                 >  2:4446698/1‑140 (MQ=255)

                                                                                                      tGGTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTg                                 >  1:1620325/1‑140 (MQ=255)

                                                                                                        gTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGa                               >  1:1783115/1‑140 (MQ=255)

                                                                                                        gTTATCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTAACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGa                               >  1:5089897/1‑140 (MQ=255)

                                                                                                           aTCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCtt                                        <  1:914376/128‑1 (MQ=255)

                                                                                                           aTCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCtt                                        >  2:914376/1‑128 (MQ=255)

                                                                                                           aTCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCtt                                        >  2:2251022/1‑128 (MQ=255)

                                                                                                           aTCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAAcc                            >  1:1213414/1‑140 (MQ=255)

                                                                                                           aTCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAAcc                            >  2:2364647/1‑140 (MQ=255)

                                                                                                           aTCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAAcc                            >  2:4193753/1‑140 (MQ=255)

                                                                                                           aTCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACCGTCGCCGCGGACAAACGGCTGGAACAGGAGCGTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCAACCCGGAAcc                            >  2:2139873/1‑140 (MQ=255)

                                                                                                            tCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCa                           >  1:438351/1‑140 (MQ=255)

                                                                                                            tCCACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCa                           >  1:4546451/1‑140 (MQ=255)

                                                                                                              cACGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAgg                         <  1:523753/140‑1 (MQ=255)

                                                                                                                cGATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGgcg                       <  1:2147313/140‑1 (MQ=255)

                                                                                                                 gATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCTGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCACgcgc                      >  2:396485/1‑140 (MQ=255)

                                                                                                                 gATACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGgcgc                      >  1:3650376/1‑140 (MQ=255)

                                                                                                                  aTACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCCAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGcgcg                     >  2:1784338/1‑140 (MQ=255)

                                                                                                                   tACGCTGCACAATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACCGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTCGAACCAGGCGCGa                    >  2:4591371/1‑140 (MQ=255)

                                                                                                                   tACGCTGCACAATTGCCAGCCCGAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGa                    >  2:5022532/1‑140 (MQ=255)

                                                                                                                      gCTGCACAATTTCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTTTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTc                 >  1:1089027/1‑140 (MQ=255)

                                                                                                                          cacaATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCt                                         >  2:3109815/1‑112 (MQ=255)

                                                                                                                          cacaATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCt             >  1:452347/1‑140 (MQ=255)

                                                                                                                          cacaATTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCt             >  2:34060/1‑140 (MQ=255)

                                                                                                                             aaTTGCCAGCCCTAATCCCGTGCCGCTCATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCCGGCGCGATTCGGCTCCg          >  1:4934765/1‑140 (MQ=255)

                                                                                                                             aaTTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCg          >  1:3736843/1‑140 (MQ=255)

                                                                                                                             aaTTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCg          >  1:2835228/1‑140 (MQ=255)

                                                                                                                             aaTTGCCAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCg          >  2:242604/1‑140 (MQ=255)

                                                                                                                                  ccAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCg     >  1:3291539/1‑140 (MQ=255)

                                                                                                                                  ccAGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCg     >  1:1167622/1‑140 (MQ=255)

                                                                                                                                    aGCCCTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCgct   >  2:1024272/1‑140 (MQ=255)

                                                                                                                                     gCCCTAATCCCGTGCCGCTAATGATGCGCGCCCTGTCGCCGCGGACACACGGCTGGACCAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCCCGATTCGGCTCCGTGCCcctt  >  2:3522704/1‑139 (MQ=255)

CCTTCTTTTGTCGTGCCCTGCGCCCGCGTTACCGGCACTGGCAGCCAGGCGCGAATGGAAAGCCCGCCCCGCTCGCTGGTGCCAAGCTCCAGCATCCCGTTATGGTTATCCACGATACGCTGCACAATTGCCAGCCTTAATCCCGTGCCGCTAATGGTGCGCGCACTGTCGCCGCGGACAAACGGCTGGAACAGGTGCTTACGTTGTTCCGGCGCAATTCCCGGACCGTCATCTTCCACCTGGAACCAGGCGCGATTCGGCTCCGTTCCGCTG  >  NZ_CP025268/3535206‑3535478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`