Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,644,586 A→G G428G (GGT→GGC)  prlC ← oligopeptidase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,644,5860AG99.2% 849.6 / ‑5.6 260G428G (GGT→GGC) prlColigopeptidase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (114/143);  minor base C (1/1);  total (115/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

CGTGACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACAT  >  NZ_CP025268/3644450‑3644722
                                                                                                                                        |                                                                                                                                        
 gTGACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTa                                                                                                                                      <  1:1786753/140‑1 (MQ=255)
   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTCCCGGGCGGTTGAAGTTACAAGTCAACTACGCGACCGGTTTTTGCACAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  2:4774697/1‑140 (MQ=255)
   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  2:3765161/1‑140 (MQ=255)
   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  1:3671331/1‑140 (MQ=255)
   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  1:381231/1‑140 (MQ=255)
   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  1:4024438/1‑140 (MQ=255)
   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  1:2876227/1‑140 (MQ=255)
   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  1:4953369/1‑140 (MQ=255)
   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  1:727539/1‑140 (MQ=255)
   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  2:2213962/1‑140 (MQ=255)
   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    <  1:1885999/140‑1 (MQ=255)
   gACCGAACTCGTGGAAGAGGGTGAGCACTGCGTCGTGAGTGAACAGCGCGGGTTTACCAGTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTGTACGGATGTGGCCTGCg                                                                                                                                    >  2:1608142/1‑140 (MQ=255)
   gACCGAACTCGTGGAAGAGGGGGATCACGTCGTCGTCAGTGAACAGCGCCGGTTGACCCTTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCTACCGGTTTTTGCAAAGAACCATCAGCTTTACCGATCTGGCCTACg                                                                                                                                    >  2:4504013/1‑140 (MQ=255)
    aCCGAACTCGTGGAAGAGGGTGAGCACTTCGTCGTGAGTGCACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGc                                                                                                                                   <  1:4771592/140‑1 (MQ=255)
    aCCGAACGCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGCCCAATCCGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGc                                                                                                                                   <  2:4367737/140‑1 (MQ=255)
     ccGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCa                                                                                                                                  <  2:1255354/140‑1 (MQ=255)
     ccGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCa                                                                                                                                  <  1:3055211/140‑1 (MQ=255)
        aaCTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGTCCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTc                                                                                                                               >  2:4566168/1‑140 (MQ=255)
        aaCTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAGCCATCAGCTTTACGCATCTGGCCTACGCAGTc                                                                                                                               >  1:4686242/1‑140 (MQ=255)
        aaCTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTc                                                                                                                               >  2:2075573/1‑140 (MQ=255)
         aCTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTca                                                                                                                              >  1:857930/1‑140 (MQ=255)
              tGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTcatccat                                                                                                                         >  2:2574328/1‑140 (MQ=255)
              tGGAAGAGGGTGATCACTTCGTCGGGAGTGAACAGCGCCGGGTTACCATTTACCGGGCGGTGGGAGTTGCGAGTGAACTACACGCCCGTTTGTGGCAAAGAACGATCAGGCTTACGCATCTGGCCTACGCAGtactgtat                                                                                                                         >  2:2909763/1‑133 (MQ=255)
                     gggTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGt                                                                                                                                >  2:1956819/1‑126 (MQ=255)
                      ggTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGccc                                                                                                                 >  2:2160160/1‑140 (MQ=255)
                      ggTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGccc                                                                                                                 >  2:3496288/1‑140 (MQ=255)
                      ggTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGccc                                                                                                                 >  1:236714/1‑140 (MQ=255)
                       gTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGTCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGcccc                                                                                                                >  1:256880/1‑140 (MQ=255)
                       gTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGcccc                                                                                                                >  1:692408/1‑140 (MQ=255)
                       gTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGcccc                                                                                                                >  1:2032602/1‑140 (MQ=255)
                        tGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCccg                                                                                                               >  2:1866525/1‑140 (MQ=255)
                          aTCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCccgcc                                                                                                             >  2:525271/1‑140 (MQ=255)
                          aTCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCccgcc                                                                                                             >  2:3458280/1‑140 (MQ=255)
                                tcgtcgTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCtt                                                                                                       >  2:3090041/1‑140 (MQ=255)
                                 cgtcgtGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTg                                                                                                      >  2:3220891/1‑140 (MQ=255)
                                 cgtcgtGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTg                                                                                                      >  1:4369200/1‑140 (MQ=255)
                                    cgtGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGttt                                                                                                   >  1:3250915/1‑140 (MQ=255)
                                       gagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCa                                                                                                <  1:1569498/140‑1 (MQ=255)
                                       gagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCa                                                                                                <  1:2177371/140‑1 (MQ=255)
                                       gagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCa                                                                                                <  1:1940136/140‑1 (MQ=255)
                                       gagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCa                                                                                                <  2:4831591/140‑1 (MQ=255)
                                        agtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCAc                                                                                               >  2:4603378/1‑140 (MQ=255)
                                         gtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACg                                                                                              <  2:4241519/140‑1 (MQ=255)
                                         gtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACg                                                                                              <  1:4322082/140‑1 (MQ=255)
                                         gtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACg                                                                                              <  1:3935857/140‑1 (MQ=255)
                                         gtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACg                                                                                              >  2:171894/1‑140 (MQ=255)
                                         gtgaAAAGCGCCGGTTTACCATTTACCGGGCGGTTGCAGTTACCAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACg                                                                                              <  1:2510490/140‑1 (MQ=255)
                                          tgaACAGCGCTGGCTTACCACTCACCGGGCGGTTGAAGTTACAAGTCAAACACGCGCCCGGTTTTTGCCAAGAACCCTCAGCTTTACGCATGTGGCCTACGCAGTCATCCATACACGCCCCGCCGCGCTTGTTTTCACgg                                                                                             <  2:3818638/140‑1 (MQ=255)
                                            aaCAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGc                                                                                           >  2:1030307/1‑140 (MQ=255)
                                             aCAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCa                                                                                          <  2:3918029/140‑1 (MQ=255)
                                             aCAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCa                                                                                          <  1:3369238/140‑1 (MQ=255)
                                               aGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATa                                                                                        <  1:3202846/140‑1 (MQ=255)
                                                 cgcCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACa                                                                                      >  2:3226717/1‑140 (MQ=255)
                                                   ccGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGa                                                                                    >  1:1317662/1‑140 (MQ=255)
                                                   ccGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGa                                                                                    >  1:3000379/1‑140 (MQ=255)
                                                    cGGTTTCCCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  1:2941248/140‑1 (MQ=255)
                                                    cGGTTTACCATTTCCCGGGCGGTTGAAGTTACATGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTAGGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  1:609352/140‑1 (MQ=255)
                                                    cGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  1:2157593/140‑1 (MQ=255)
                                                    cGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  1:2542618/140‑1 (MQ=255)
                                                    cGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  1:509820/140‑1 (MQ=255)
                                                    cGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  2:4954279/140‑1 (MQ=255)
                                                      gTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCg                                                                                 <  2:3104571/140‑1 (MQ=255)
                                                             aTTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCCGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGa                                                                          <  1:4014057/140‑1 (MQ=255)
                                                             aTTTACCGGGCGGTTGAAGTTACAAGCAAAATACGCGACCGGTTTTTCCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGa                                                                          <  2:2461926/140‑1 (MQ=255)
                                                              tttACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGaa                                                                         >  2:589074/1‑140 (MQ=255)
                                                               ttACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAg                                                                        <  2:692408/140‑1 (MQ=255)
                                                               ttACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAg                                                                        <  2:1468564/140‑1 (MQ=255)
                                                                tACCGGGCGGTTGAAGTTACAAGTCTAATACGCGACCGGGTTTTCCAAAGAACCATCAGCTTTACGCATCTGGGCTACGCAGTCATCCAAACACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGc                                                                       >  2:3915013/1‑140 (MQ=255)
                                                                tACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGc                                                                       >  1:443706/1‑140 (MQ=255)
                                                                 aCCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCt                                                                      >  2:1193228/1‑140 (MQ=255)
                                                                     ggCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcg                                                                  >  1:1273743/1‑140 (MQ=255)
                                                                     ggCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcg                                                                  >  2:53414/1‑140 (MQ=255)
                                                                      gCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAACTTTACGCATCTGGCCTACGCAGTCATCCATCCACACACCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACggc                                                                 >  2:3265225/1‑140 (MQ=255)
                                                                       cGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcgcg                                                                <  1:1134962/140‑1 (MQ=255)
                                                                                  ataaGTCAAATACGCGACCCGCTTTTGCACCGAACCCCCCGCTTAACGCATCTGGCCTACGCAATCACCCATCCACGCCCCGCCGCGATTGTTTTCACCGGCATACAGATCGACGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  2:4340962/138‑1 (MQ=255)
                                                                                  aCAAGTCAAATACGCGACCGGTTTTTGCAAAGACCCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  1:2767023/140‑1 (MQ=255)
                                                                                  aCAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  2:84368/140‑1 (MQ=255)
                                                                                  aCAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  2:3356948/140‑1 (MQ=255)
                                                                                  aCAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  2:3330130/140‑1 (MQ=255)
                                                                                  aCAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  1:1795404/140‑1 (MQ=255)
                                                                                  aCAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  2:2144064/140‑1 (MQ=255)
                                                                                  aCAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  2:18250/140‑1 (MQ=255)
                                                                                    aaGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAttt                                                   >  1:5042101/1‑140 (MQ=255)
                                                                                       tCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTa                                                                                                                                      >  1:3335955/1‑54 (MQ=255)
                                                                                       tCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCg                                                <  1:5154370/140‑1 (MQ=255)
                                                                                            taccccaCCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGGTATTTTCGTCATa                                           <  2:3961104/134‑1 (MQ=255)
                                                                                            tACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATa                                           <  2:1554484/140‑1 (MQ=255)
                                                                                            tACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATa                                           <  1:4239543/140‑1 (MQ=255)
                                                                                            tACGCGACCGGCTTTTGCAAAGACCCATCAGCTCTACGCGTCTGGCCTACGCAGTCAGCCATCCACGCCCCGCCGCGCTAGTTTTCAAGGGCACACAGATCGCGGTAGAAGCTAACGCGCAGTTCGTTATTTTCGTCATa                                           <  2:1116237/140‑1 (MQ=255)
                                                                                                     ggTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCgaa                                  <  1:3465238/140‑1 (MQ=255)
                                                                                                      gTTTTTGCAAAGAACCATCAGCTTTCCGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCgaag                                 <  2:750171/140‑1 (MQ=255)
                                                                                                      gTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCgaag                                 <  1:2237937/140‑1 (MQ=255)
                                                                                                             cAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTa                          <  2:2876227/140‑1 (MQ=255)
                                                                                                              aaaGAACCATCCGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTAc                         <  1:3146346/140‑1 (MQ=255)
                                                                                                                   aCCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCg                    <  2:3794500/140‑1 (MQ=255)
                                                                                                                           cTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAg            <  2:4024438/140‑1 (MQ=255)
                                                                                                                              tACGCATCTGGCTTACGCAGACATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACa         >  2:5138931/1‑140 (MQ=255)
                                                                                                                              tACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATCCAGTTCGAAGAAACGTACATCCGGATGCCAGACa         >  1:117195/1‑140 (MQ=255)
                                                                                                                                 gCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCa      >  1:3599771/1‑140 (MQ=255)
                                                                                                                                  cATCTGGCCTACGCAGTCATCCCTCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCaa     <  2:3442745/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
CGTGACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACAT  >  NZ_CP025268/3644450‑3644722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑