BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,963,836	C→G	A414G (GCC→GGC)	rep →	DNA helicase Rep

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,963,836	0	C	G	99.7%	1112.8 / NA	333	A414G (GCC→GGC)	rep	DNA helicase Rep
Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (153/179); minor base A (0/1); total (153/180)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 341 total aligned reads displayed.

TACAAAATATCTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGCCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGACCCGCTTCACTCA  >  NZ_CP025268/3963700‑3963968

tACAAAATATCTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGgccc                                                                                                                                   >  1:3399489/1‑140 (MQ=255)

tACAAAATATCTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGgccc                                                                                                                                   >  2:3646195/1‑140 (MQ=255)

tACAAAATATCTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGgccc                                                                                                                                   >  2:2493042/1‑140 (MQ=255)

tACAAAATATCTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACGTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTGCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGgccc                                                                                                                                   >  2:1908940/1‑140 (MQ=255)

  cAAGATATCTGGTGGTACGTCGTTTTTCTCTCGTCCTGAGATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCGGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCgg                                                                                                                                 <  2:2955084/140‑1 (MQ=255)

   aaaaTATCTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGc                                                                                                                                <  1:3046666/140‑1 (MQ=255)

     aaTATCTGGTGGTACGTCGTTTTTCTCTCTTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTa                                                                                                                              >  2:4847256/1‑140 (MQ=255)

      atatCTGGTGGTACGTCGTTTTTCTCTCGTCCTGCAATCAAGGACTTGCTTGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCCTTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTAc                                                                                                                             <  2:1509421/140‑1 (MQ=255)

      atatCTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTAc                                                                                                                             <  2:4932163/140‑1 (MQ=255)

      atatCTGGCGGTACGTCGTTTTTCTCTCGTCCTCAACTCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGCCGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTAc                                                                                                                             <  2:1148413/140‑1 (MQ=255)

          cTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTg                                                                                                                         >  1:5194912/1‑140 (MQ=255)

          cTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTg                                                                                                                         >  1:1039064/1‑140 (MQ=255)

          cTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTg                                                                                                                         >  1:1725718/1‑140 (MQ=255)

          cTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTg                                                                                                                         >  2:1823456/1‑140 (MQ=255)

          cTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTg                                                                                                                         >  1:2362303/1‑140 (MQ=255)

             gtggtACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGaaa                                                                                                                      >  2:3445243/1‑140 (MQ=255)

             gtggtACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGaaa                                                                                                                      >  1:1368658/1‑140 (MQ=255)

                 tACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGc                                                                                                                  >  1:805589/1‑140 (MQ=255)

                 tACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGc                                                                                                                  >  1:3252016/1‑140 (MQ=255)

                   cgtcgtTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTg                                                                                                                >  1:843197/1‑140 (MQ=255)

                       gtTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTg                                                                                                            >  1:2587161/1‑140 (MQ=255)

                        tttttCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGa                                                                                                           <  2:2262435/140‑1 (MQ=255)

                          tttCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCCTTTCTGCGTATCGTTAACACGCCGAAGCGCGAGATCGGCCCGGCCACGCCGACAAAGCTGGGTGAgg                                                                                                         >  2:1312442/1‑139 (MQ=255)

                                 cGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGAt                                                                                                  <  1:397313/140‑1 (MQ=255)

                                  gTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATg                                                                                                 >  1:1819084/1‑140 (MQ=255)

                                     cTGAACTCAAGGACTTGCTGAGATATCTGCGCGTGATGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCCTTAACACGCCGAAGCGAGAGAGTGGACCGGGTACGCTGAAAAGGCTGGGTGAGTGGGCGATGAcg                                                                                              <  2:4588253/140‑1 (MQ=255)

                                     cTGAAATCACGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCAGTTCTGCGTATCGTTACCACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGAcg                                                                                              <  1:1101396/140‑1 (MQ=255)

                                     cTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGAcg                                                                                              <  2:4299816/140‑1 (MQ=255)

                                     cTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGAcg                                                                                              <  2:4388942/140‑1 (MQ=255)

                                     cTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGAcg                                                                                              <  2:928560/140‑1 (MQ=255)

                                     cTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGAcg                                                                                              <  1:3904508/140‑1 (MQ=255)

                                       gAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGAcgcg                                                                                            <  1:2447466/140‑1 (MQ=255)

                                        aaaTCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACgcgc                                                                                           <  1:983794/140‑1 (MQ=255)

                                              aGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATaaa                                                                                     <  1:2923514/140‑1 (MQ=255)

                                                 aCTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGc                                                                                  <  1:590159/140‑1 (MQ=255)

                                                    tGCTGGCTTATCTGCGCGTGCTGACTAACCCGGCCGATGACGGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATAACGCGCAATAAAAGCacg                                                                               >  2:3917117/1‑138 (MQ=255)

                                                     gCTGGCTTATCTGCGCGAGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATAGTTAACACGCCGAAGCGAGAGATTGGCCCGGCCACGCTGAAAAAGCTGGGTGAGGGGGCGATGACGCGCCATAAAAGCATGt                                                                              >  2:4330466/1‑140 (MQ=255)

                                                            tATCTGCGCGTGCTGACTAGCCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAAGAAAAGCATGTTTACCGc                                                                       <  1:925609/140‑1 (MQ=255)

                                                            tATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGc                                                                       <  1:4301805/140‑1 (MQ=255)

                                                              tCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGGGAGTGGGCGATGACGCGCAATAAGAGCATGTTTACCGCCa                                                                     >  2:4709135/1‑140 (MQ=255)

                                                                   gcgTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCttt                                                                >  1:1968284/1‑140 (MQ=255)

                                                                       gCTGACTACCCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGata                                                            <  2:1039201/140‑1 (MQ=255)

                                                                       gCTGACTAACCCGGGCGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGGGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGata                                                            <  2:615922/140‑1 (MQ=255)

                                                                       gCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGata                                                            <  2:4938027/140‑1 (MQ=255)

                                                                       gCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGata                                                            >  2:73592/1‑140 (MQ=255)

                                                                       gCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCCGGGTGTGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGata                                                            <  2:2882366/140‑1 (MQ=255)

                                                                       gCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCCGGGTGCGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGata                                                            <  2:2881896/140‑1 (MQ=255)

                                                                        cTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGatat                                                           <  2:2993271/140‑1 (MQ=255)

                                                                        cTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGatat                                                           <  1:1603621/140‑1 (MQ=255)

                                                                        cTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGatat                                                           >  1:476195/1‑140 (MQ=255)

                                                                              aaCCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCt                                                     <  2:5184703/140‑1 (MQ=255)

                                                                               aCCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTg                                                    >  1:699509/1‑140 (MQ=255)

                                                                                cccGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGa                                                   >  1:3790975/1‑140 (MQ=255)

                                                                                  cGGACGATGACACCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGt                                                 <  1:4448647/140‑1 (MQ=255)

                                                                                     aCGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGCGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAg                                              >  2:3738079/1‑140 (MQ=255)

                                                                                     aCGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAg                                              <  2:270519/140‑1 (MQ=255)

                                                                                     aCGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAg                                              >  1:2224214/1‑140 (MQ=255)

                                                                                      cGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGa                                             <  2:2697872/140‑1 (MQ=255)

                                                                                               cgcATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCg                                    >  1:2804517/1‑140 (MQ=255)

                                                                                               cgcATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTCGCCCGGGTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCCGCTTTGATATGGGCCTGAGTCAGACGCCTAGCg                                    >  2:3302714/1‑140 (MQ=255)

                                                                                               cgcATTTCTGCGGATGGTTAACACGCCGAAGCGGGAGATTGGCCCGGCTACGCGGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTGACCGCCAGCTTTGATAGGGGCCTGAGTCAGACGCTTAGCg                                    <  2:880487/140‑1 (MQ=255)

                                                                                                gcttttCTGCGGATCGTTAACGCGCCGAAGCGAGTCATTGGCCAGGCTACGCTGAAACGTCGGTGTGAGTGGGCGATGACGCGCAATTCAAGCGCGGTTACCGTCAGCTTTGAGATGGGCCTGAGGCAGACGCTTAgagt                                   <  2:444782/137‑4 (MQ=255)

                                                                                                 cATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTCCGCTGAAAACGCTGGGTGCGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGa                                  <  2:3867414/140‑1 (MQ=255)

                                                                                                 cATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGa                                  <  1:146224/140‑1 (MQ=255)

                                                                                                 cATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGa                                  <  1:2006628/140‑1 (MQ=255)

                                                                                                 cATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGa                                  >  1:5070500/1‑140 (MQ=255)

                                                                                                   tttCTGCGTATCGTTAACACGCCGTAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACg                                <  1:1868772/140‑1 (MQ=255)

                                                                                                   tttCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACg                                <  2:395297/140‑1 (MQ=255)

                                                                                                   tttCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACg                                <  1:1715128/140‑1 (MQ=255)

                                                                                                   tttCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACg                                <  1:1278775/140‑1 (MQ=255)

                                                                                                   tttCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACg                                <  2:1976963/140‑1 (MQ=255)

                                                                                                   tttCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGCGCCTGAGTCAGACGCTTAGCGGACg                                <  2:1415591/140‑1 (MQ=255)

                                                                                                    ttCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGt                               >  1:5010961/1‑140 (MQ=255)

                                                                                                    ttCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGt                               <  2:476195/140‑1 (MQ=255)

                                                                                                         cGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTa                          >  1:4182764/1‑140 (MQ=255)

                                                                                                         cGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTa                          >  1:4632154/1‑140 (MQ=255)

                                                                                                         cGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTa                          >  2:1358450/1‑140 (MQ=255)

                                                                                                           tATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATg                        <  1:3684049/140‑1 (MQ=255)

                                                                                                           tATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATg                        <  2:5049326/140‑1 (MQ=255)

                                                                                                           tATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATg                        <  1:3502562/140‑1 (MQ=255)

                                                                                                              cGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAg                     >  2:1647678/1‑140 (MQ=255)

                                                                                                              cGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAg                     >  1:3140281/1‑140 (MQ=255)

                                                                                                              cGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAg                     >  1:2783107/1‑140 (MQ=255)

                                                                                                              cGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTGGGTATGGGCCTGAGTCAGACGCTTAGCGGACGTGTTTATGAAg                     >  2:4183254/1‑140 (MQ=255)

                                                                                                              cGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGGGAGTGGGCGATGACGCGCAATAAAAGCATGTTTAGCGCCAGCTTTGATATGGGCCTGAGTCAGACGCGTAGCGGACGTGGTTATGaat                     >  2:3236538/1‑139 (MQ=255)

                                                                                                                ttAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCa                   >  2:173951/1‑140 (MQ=255)

                                                                                                                ttAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCa                   >  2:2352037/1‑140 (MQ=255)

                                                                                                                ttAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCa                   >  2:781424/1‑140 (MQ=255)

                                                                                                                 tAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCAt                  >  1:3344256/1‑140 (MQ=255)

                                                                                                                       gCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGAccc            <  1:2893197/140‑1 (MQ=255)

                                                                                                                       gCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGAccc            <  2:2362303/140‑1 (MQ=255)

                                                                                                                       gCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGAGGGCGATGACGCGCAATAAAAGCGTGTTTACCGCCAGCTTTGATATGGGCCTGAGACAGACGCTTAGCGGACGTGGTTATGAAGAATTGAccc            >  2:3030579/1‑140 (MQ=255)

                                                                                                                        ccGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGACCCg           >  1:4535481/1‑140 (MQ=255)

                                                                                                                        ccGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGACCCg           <  2:2577999/140‑1 (MQ=255)

                                                                                                                         cGAAGCGAGAGATTGGCCCGGCTGCGCTGAAAAAGCTGGGGGAGGGGGCGATGACGCGCAAGAAAAGCATGTTGACCGCCAGCGTTGATATGGTCCTGAGTCAGACGCTTAGCGGACGTGGTGAGGAAGCAGTggctcgg          >  2:42121/1‑134 (MQ=255)

                                                                                                                          gAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGACCCGCt         <  2:3095624/140‑1 (MQ=255)

                                                                                                                          gAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGACCCGCt         <  2:2620966/140‑1 (MQ=255)

                                                                                                                          gAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGACCCGCt         >  2:3481915/1‑140 (MQ=255)

                                                                                                                          gAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGACCCGCt         <  1:3482764/140‑1 (MQ=255)

                                                                                                                                 gagaTTGGCCCGGCTACGCTTAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGGCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGACCCGGTtcactca  <  2:2073535/140‑1 (MQ=255)

TACAAAATATCTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTGACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGCCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGACCCGCTTCACTCA  >  NZ_CP025268/3963700‑3963968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`