BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,971,934	C→T	pseudogene (985/1048 nt)	wzzE →	ECA polysaccharide chain length modulation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,971,934	0	C	T	100.0%	1483.8 / NA	396	pseudogene (985/1048 nt)	wzzE	ECA polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (208/188); total (208/188)

Only 100 of 396 total aligned reads displayed.

CAGAATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTA  >  NZ_CP025268/3971799‑3972070

 aGAATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGc                                                                                                                                     >  1:3615497/1‑140 (MQ=255)

  gAATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTACAACGCGATAGCCCACGTCGTGCCGTCCTGATGATTATGTGGGGCa                                                                                                                                    <  1:1258450/140‑1 (MQ=255)

   aaTCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTGTTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCAt                                                                                                                                   <  2:4147512/140‑1 (MQ=255)

   aaTCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCAt                                                                                                                                   >  2:1085695/1‑140 (MQ=255)

   aaTCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCAt                                                                                                                                   >  1:2593434/1‑140 (MQ=255)

   aaTCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCAt                                                                                                                                   >  1:3736059/1‑140 (MQ=255)

   aaTCGGCCAATGTTAAACACCCTGAATGTTGGTCCACCCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCAt                                                                                                                                   <  1:331418/140‑1 (MQ=255)

        ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCg                                                                                                                              >  1:1400441/1‑140 (MQ=255)

        ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCg                                                                                                                              <  1:538800/140‑1 (MQ=255)

        ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCg                                                                                                                              >  1:1664718/1‑140 (MQ=255)

        ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCg                                                                                                                              >  2:4747931/1‑140 (MQ=255)

        ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCg                                                                                                                              >  1:3405016/1‑140 (MQ=255)

        ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACATCGTGCCTTCCTGATGATTATGTGGGGCATTGTCg                                                                                                                              >  1:1102946/1‑140 (MQ=255)

             tGTTAACCACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTCCGCCGGAAGAACCGGTAAAGCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCgggggg                                                                                                                         <  2:4616656/140‑1 (MQ=255)

             tGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCgggggg                                                                                                                         <  1:3679485/140‑1 (MQ=255)

             tGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCgggggg                                                                                                                         <  1:31323/140‑1 (MQ=255)

             tGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCgggggg                                                                                                                         >  1:3860619/1‑140 (MQ=255)

             tGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCgggggg                                                                                                                         >  1:4154346/1‑140 (MQ=255)

             tGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGAGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTCgggggg                                                                                                                         <  1:3123943/140‑1 (MQ=255)

                  aaCACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGAt                                                                                                                   <  2:1335121/140‑1 (MQ=255)

                   acacCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATc                                                                                                                  >  1:37885/1‑140 (MQ=255)

                   acacCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATc                                                                                                                  >  2:3820579/1‑140 (MQ=255)

                       ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCTCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATAgggg                                                                                                              >  1:3989935/1‑140 (MQ=255)

                       ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCgggg                                                                                                              >  2:4447851/1‑140 (MQ=255)

                       ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCgggg                                                                                                              >  1:605622/1‑140 (MQ=255)

                       ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCgggg                                                                                                              >  1:1789407/1‑140 (MQ=255)

                       ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCgggg                                                                                                              >  2:5181481/1‑140 (MQ=255)

                       ccTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCgggg                                                                                                              >  1:335958/1‑140 (MQ=255)

                        cTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGc                                                                                                             >  1:4092906/1‑140 (MQ=255)

                        cTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGGAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGc                                                                                                             >  1:4204078/1‑140 (MQ=255)

                         tGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCt                                                                                                            >  2:2557611/1‑140 (MQ=255)

                         tGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCt                                                                                                            >  1:1906330/1‑140 (MQ=255)

                         tGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCt                                                                                                            >  2:406808/1‑140 (MQ=255)

                           aaTGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTgg                                                                                                          >  1:3466142/1‑140 (MQ=255)

                            aTGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGgt                                                                                                         >  2:181403/1‑140 (MQ=255)

                             tGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAGCCGGTAAAACGCTATAGCTCCCGTCGTGCCTTCCTGCCGATTATGTGGAGCATTGTC‑GGGGGGATGATCGGGGCTGgtg                                                                                                        <  2:3414562/140‑1 (MQ=255)

                                 ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTGCTGATGATTACGTGGGGCATTG‑GTGGGGGGCGGATCGGGGCTGGTGTCgg                                                                                                    >  2:3506612/1‑139 (MQ=255)

                                 ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGc                                                                                                    >  1:4433376/1‑140 (MQ=255)

                                 ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGc                                                                                                    >  1:1363454/1‑140 (MQ=255)

                                   cccAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGCACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCAt                                                                                                  <  2:1670471/139‑1 (MQ=255)

                                     cAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTa                                                                                                >  2:3601761/1‑140 (MQ=255)

                                     cAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGGCGCATTa                                                                                                >  2:870577/1‑140 (MQ=255)

                                       aCCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAAc                                                                                              >  1:2296299/1‑140 (MQ=255)

                                         ccTGGATCCGCGTTTTCAGACCTATCGCGATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTACCTGATGATTATGTGGGGCATTGTC‑GGGGGGCGGATCGGGGCTGGTGTCGCATTAAccc                                                                                            >  1:1545542/1‑140 (MQ=255)

                                             gATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACccgccg                                                                                        >  1:4875724/1‑140 (MQ=255)

                                                 cgcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATGGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGc                                                                                    >  2:3300363/1‑140 (MQ=255)

                                                  gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt                                                                                   >  2:5074238/1‑140 (MQ=255)

                                                  gcgTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt                                                                                   >  1:2677212/1‑140 (MQ=255)

                                                   cgTTTTCAGACCTATCGCTATTCGCGTAGGCGGTAAGACCCGGTAAAACGCGATAGCCCACGGCGTGCCTGACTGATCATTACGTGTGGCGTTGTC‑GGGGGGCTGATCGGGGCTAGTGTCTCATCAACCCACCGATGCTc                                                                                  >  2:1451365/1‑140 (MQ=255)

                                                     ttttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa                                                                                <  1:2772492/140‑1 (MQ=255)

                                                     ttttCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa                                                                                <  2:488484/140‑1 (MQ=255)

                                                        tCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAAt                                                                             <  1:2648707/140‑1 (MQ=255)

                                                             ccTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGCTCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATagcaa                                                                        <  2:658855/140‑1 (MQ=255)

                                                             ccTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTCCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATagcaa                                                                        <  1:5003591/140‑1 (MQ=255)

                                                               tATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACTTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGTGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAgcaaca                                                                      <  1:3099446/140‑1 (MQ=255)

                                                               tATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAgcaaca                                                                      <  1:3529635/140‑1 (MQ=255)

                                                                aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAacac                                                                     >  1:4747295/1‑140 (MQ=255)

                                                                 tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAAGAct                                                                    >  2:3367281/1‑140 (MQ=255)

                                                                 tCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGGGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACAct                                                                    >  2:2339766/1‑140 (MQ=255)

                                                                    cTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACActgct                                                                 <  1:511032/140‑1 (MQ=255)

                                                                    cTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACActgct                                                                 <  1:2038642/140‑1 (MQ=255)

                                                                    cTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACActgct                                                                 <  1:88159/140‑1 (MQ=255)

                                                                         tGCGTACGCCGGAAGAACCGGTAAAACGCGAAAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGt                                                            <  1:2419710/140‑1 (MQ=255)

                                                                           cGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGa                                                          >  1:2520910/1‑140 (MQ=255)

                                                                               cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAgcgc                                                      >  1:2372970/1‑140 (MQ=255)

                                                                               cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAgcgc                                                      >  1:5141980/1‑140 (MQ=255)

                                                                                        aaCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCt                                             <  1:3723561/140‑1 (MQ=255)

                                                                                             gTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg                                        <  2:90576/140‑1 (MQ=255)

                                                                                             gTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg                                        <  2:4202250/140‑1 (MQ=255)

                                                                                             gTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg                                        <  2:1243012/140‑1 (MQ=255)

                                                                                               aaaaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                      <  1:725751/140‑1 (MQ=255)

                                                                                                  aCGCGATAGCCCACGTCGTGCCTTCCTGGTGAGTGGGTGGGGCGTTGTCGGGGGGG‑TGCGAGGGGCTGGGGTGGCATTGACCCGCCGTTGCTCGAAATAGACACGCGGATGCGGTGAGCGCAAAGGCGCTCGCCGCttct                                   >  2:2442336/1‑138 (MQ=255)

                                                                                                  aCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAt                                   >  2:4740984/1‑140 (MQ=255)

                                                                                                  aCGAGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGAGCGGGGCTGGTGTCGCAGTAACCCGCCGTGGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTAt                                   <  2:2463310/140‑1 (MQ=255)

                                                                                                         aGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAaga                            <  1:3804167/140‑1 (MQ=255)

                                                                                                         aGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAaga                            <  2:3061418/140‑1 (MQ=255)

                                                                                                             cACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAAt                        <  2:3931645/140‑1 (MQ=255)

                                                                                                              aCGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATc                       >  2:3188138/1‑140 (MQ=255)

                                                                                                               cgtcgtGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCg                      >  2:590235/1‑140 (MQ=255)

                                                                                                                gtcgtGCCTTCCTGATGACTACGTGGGGCATCGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGATGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGCGAGTcca                     >  2:4327187/1‑138 (MQ=255)

                                                                                                                  cgtGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtg                   >  2:3682090/1‑140 (MQ=255)

                                                                                                                    tGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtgtg                 >  1:1442536/1‑140 (MQ=255)

                                                                                                                     gCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGa                >  1:4475995/1‑140 (MQ=255)

                                                                                                                     gCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGa                >  1:287323/1‑140 (MQ=255)

                                                                                                                     gCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGa                <  1:2834159/140‑1 (MQ=255)

                                                                                                                         tCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGt            >  1:2972786/1‑140 (MQ=255)

                                                                                                                         tCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGt            >  2:2811940/1‑140 (MQ=255)

                                                                                                                          ccTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa           >  1:4362108/1‑140 (MQ=255)

                                                                                                                          ccTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa           <  1:4599465/140‑1 (MQ=255)

                                                                                                                          ccTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa           <  2:765003/140‑1 (MQ=255)

                                                                                                                          ccTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa           <  2:205916/140‑1 (MQ=255)

                                                                                                                           cTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTac          >  1:1024304/1‑140 (MQ=255)

                                                                                                                             gatgatTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTacta        <  1:2157085/140‑2 (MQ=255)

                                                                                                                             gatgatAAGGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAACTAGCAACACTGCTGCGGTACGCGCAAGGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGACAGTactg        <  2:1197695/140‑1 (MQ=255)

                                                                                                                              atgatTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga       <  1:246646/140‑1 (MQ=255)

                                                                                                                                gatTATGTGGGGCATTGTC‑GGGGGGCGGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTGGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGGTTATTCGAAGAGAAACGATGTGAAAGTactggaa     >  2:2616549/1‑137 (MQ=255)

                                                                                                                                 atTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgactg    >  2:1545142/1‑140 (MQ=255)

                                                                                                                                   tATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTa  >  1:807956/1‑140 (MQ=255)

                                                                                                                                   tATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTa  >  2:217023/1‑140 (MQ=255)

                                                                                                                                   tATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTa  >  2:2978453/1‑140 (MQ=255)

CAGAATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTA  >  NZ_CP025268/3971799‑3972070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`