BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,215,602	T→C	148 (TAA→TAG)	yjaB ←	acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,215,602	0	T	C	99.3%	1003.6 / NA	306	148 (TAA→TAG)	yjaB	acetyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (185/118); minor base G (2/0); total (188/118)
Fisher's exact test for biased strand distribution p-value = 5.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 306 total aligned reads displayed.

CCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTG  >  NZ_CP025268/4215467‑4215734

ccAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACg                                                                                                                                  <  1:541887/140‑1 (MQ=255)

ccAGTTCCACATCCATGCGCAATCAGCGGTACTCAGCGATAGGGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACg                                                                                                                                  <  2:1035732/140‑1 (MQ=255)

  atgtCCCCATCCATGCGGAGTTAGCGGTACTCAGTGGTAGGTCGCTCGTGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTTGCCCTGACGTGCAGCCCACGGGGATGAGAAAAAATCGCCTACGca                                                                                                                                <  2:762782/137‑2 (MQ=255)

   gTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGccc                                                                                                                               <  1:4565449/140‑1 (MQ=255)

    ttCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGcccc                                                                                                                              >  1:4797296/1‑140 (MQ=255)

    ttCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGcccc                                                                                                                              >  1:1724887/1‑140 (MQ=255)

    ttCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGCTGGGTACGAAACCTGAAGTTCACCTCACCGGGATGAGAAAAAATCGCCTACGcccc                                                                                                                              >  2:1455690/1‑140 (MQ=255)

            ccATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACg                                                                                                                      <  1:1341595/140‑1 (MQ=255)

            ccATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACg                                                                                                                      <  2:1797627/140‑1 (MQ=255)

              aTGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGcc                                                                                                                    <  1:5142013/140‑1 (MQ=255)

               tGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCa                                                                                                                   >  1:2150199/1‑140 (MQ=255)

               tGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCa                                                                                                                   >  1:1304694/1‑140 (MQ=255)

               tGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCa                                                                                                                   >  2:3617383/1‑140 (MQ=255)

               tGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAAACTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCa                                                                                                                   >  2:3791030/1‑140 (MQ=255)

                gcgcAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTAAGCCCCCACATACGCCAg                                                                                                                  >  1:3411770/1‑140 (MQ=255)

                      tCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTTAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCGCATACGCCAGATTCAg                                                                                                            >  1:2865373/1‑140 (MQ=255)

                      tCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCGTACTCCCCCACATACGCCAGATTGAg                                                                                                            >  2:422892/1‑140 (MQ=255)

                      tCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCcaca                                                                                                                          <  1:2003233/126‑1 (MQ=255)

                      tCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAg                                                                                                            >  2:3302156/1‑140 (MQ=255)

                       cAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTCACCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGCTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGc                                                                                                           <  2:4524608/140‑1 (MQ=255)

                       cAGCGGTACGCAGTGAGTCGGCGGACTTGGCGCTGCTGAAGCAGAAATAATCGTGTCACCATTGGTGGCTACTAAACCGGACGTGCAGCCCACCGCGATGAGAAAAAATCGCCTACACACCCACATACGCCAGATTCAGc                                                                                                           <  2:1545827/140‑1 (MQ=255)

                        aGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCa                                                                                                          <  2:1595620/140‑1 (MQ=255)

                                  aGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACgg                                                                                                >  2:169650/1‑140 (MQ=255)

                                   gTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGt                                                                                               <  2:2533130/140‑1 (MQ=255)

                                      aTAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATGGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTc                                                                                            >  2:4100714/1‑140 (MQ=255)

                                       tAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGCTACGGTTTcc                                                                                           >  2:5164896/1‑140 (MQ=255)

                                       tAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTcc                                                                                           >  1:626572/1‑140 (MQ=255)

                                       tAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTcc                                                                                           >  1:4871919/1‑140 (MQ=255)

                                       tAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTcc                                                                                           >  1:1003866/1‑140 (MQ=255)

                                       tAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTcc                                                                                           >  1:4051588/1‑140 (MQ=255)

                                       tAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTcc                                                                                           >  1:3935461/1‑140 (MQ=255)

                                       tAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTcc                                                                                           >  1:123669/1‑140 (MQ=255)

                                          tGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCa                                                                                        >  2:612732/1‑140 (MQ=255)

                                          tGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCa                                                                                        <  2:3167862/140‑1 (MQ=255)

                                          tGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTCTCCCCa                                                                                        >  2:4718399/1‑140 (MQ=255)

                                            cGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCaaa                                                                                      <  2:705135/140‑1 (MQ=255)

                                            cGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCaaa                                                                                      >  1:4195863/1‑140 (MQ=255)

                                            cGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCaaa                                                                                      <  2:4827424/140‑1 (MQ=255)

                                              gTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCa                                                                                                          >  2:2332125/1‑118 (MQ=255)

                                              gTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCa                                                                                                          <  1:2331877/118‑1 (MQ=255)

                                              gTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATc                                                                                    >  1:3211526/1‑140 (MQ=255)

                                              gTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATc                                                                                    >  1:175204/1‑140 (MQ=255)

                                              gTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATc                                                                                    >  1:3965284/1‑140 (MQ=255)

                                              gTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATc                                                                                    >  1:4501407/1‑140 (MQ=255)

                                              gTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATc                                                                                    >  2:3949894/1‑140 (MQ=255)

                                              gTCATGGCAATGCTTAAGCAGAAATAAGCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATc                                                                                    >  2:1984706/1‑140 (MQ=255)

                                                 aTGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTc                                                                                 <  2:1299931/140‑1 (MQ=255)

                                                 aTGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTc                                                                                 <  1:2726337/140‑1 (MQ=255)

                                                   ggCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCa                                                                               >  2:2401785/1‑140 (MQ=255)

                                                    gCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTGCCCCAAATCGTCCAc                                                                              >  2:89121/1‑140 (MQ=255)

                                                        tGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCa                                                                          <  2:588445/140‑1 (MQ=255)

                                                        tGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGGACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGAGTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCa                                                                          >  2:1417925/1‑140 (MQ=255)

                                                         gCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGAGTCAGCAACGGACACGGCTTCCCCAAATCGTCCACCTCag                                                                         >  2:4980876/1‑140 (MQ=255)

                                                         gCTTAAGCAGAAATAATCGTCTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGCGATGAGAAAAAATCGCCTACGCTCCCACATACGCCAGAGTCAGCAACGGATACGGTTTCCCCAAATTGTCAACCTCag                                                                         >  2:35272/1‑140 (MQ=255)

                                                          cTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCaga                                                                        <  1:3380561/140‑1 (MQ=255)

                                                           ttAAGCAGAGATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCagag                                                                       <  1:3373099/140‑1 (MQ=255)

                                                           ttAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCagag                                                                       <  2:4019497/140‑1 (MQ=255)

                                                           ttAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCagag                                                                       <  1:1432320/140‑1 (MQ=255)

                                                             aaGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCg                                                                     <  1:3740688/140‑1 (MQ=255)

                                                              aGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGt                                                                    >  1:1635411/1‑140 (MQ=255)

                                                               gCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTc                                                                   >  2:2649136/1‑140 (MQ=255)

                                                                   aaaTAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGt                                                               >  1:3597267/1‑140 (MQ=255)

                                                                   aaaTAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGt                                                               <  1:4694986/140‑1 (MQ=255)

                                                                   aaaTAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGt                                                               <  1:3301552/140‑1 (MQ=255)

                                                                   aaaTAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGt                                                               <  2:220282/140‑1 (MQ=255)

                                                                   aaaTAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGt                                                               <  1:3131706/140‑1 (MQ=255)

                                                                   aaaTAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGAGGAGAGAAAATCGCCTACGCCCCCACATACGCCATGCTCAGAAACGGATACGGCTTCCCAAAATCATCCACCTCAGAGCGTCCCGt                                                               >  2:1482063/1‑140 (MQ=255)

                                                                   aaaTAATCGTGTCACCATTCGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTTCCCAAATCGTCCACCTCAGAGCGTCCCGt                                                               >  2:3643008/1‑140 (MQ=255)

                                                                    aataatCTTGTCCCCATTGGTGGGTCCTAAACCTGAAGTTCAGCCCACCGGGATGAGCAAAAATCGCCTACGCCCCCACATCCGCCCGATTCAGCAACGGATACGGTTTCCCCACATCGTCCCCCTCAGAGCGTCCCGTa                                                              <  2:3053304/140‑1 (MQ=255)

                                                                        atCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCt                                                          >  1:981648/1‑140 (MQ=255)

                                                                             gtCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCTCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTaaaa                                                     >  1:779895/1‑140 (MQ=255)

                                                                             gtCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTaaaa                                                     >  1:2756219/1‑140 (MQ=255)

                                                                             gtCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTaaaa                                                     >  1:3239938/1‑140 (MQ=255)

                                                                             gtCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAACCGTCCACCTCAGAGCGTCCCGTAACCTTaaaa                                                     >  2:1153648/1‑140 (MQ=255)

                                                                               cACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAAcc                                                   <  2:2958342/140‑1 (MQ=255)

                                                                                 ccgttGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                                 <  2:2062772/137‑1 (MQ=255)

                                                                                 ccATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                                 <  2:52884/140‑1 (MQ=255)

                                                                                 ccATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCa                                                 <  2:4423709/140‑1 (MQ=255)

                                                                                    ttGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCGAAGAACCCAccc                                              >  2:2711599/1‑139 (MQ=39)

                                                                                    ttGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGACTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACct                                              <  2:1245522/140‑1 (MQ=255)

                                                                                    ttGGTCGGTACTAAACCTGAAGTTCAGCCCCCCGGGATGAGAACAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAACCCCACct                                              <  1:2472904/140‑1 (MQ=39)

                                                                                         gggTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTta                                         <  2:3286383/140‑1 (MQ=255)

                                                                                            tACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACgg                                                                                                >  2:2835112/1‑82 (MQ=255)

                                                                                            tACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAg                                      >  1:3763121/1‑140 (MQ=255)

                                                                                            tACTAAACCTGAAATTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACAGACGCCAGATTCAGCAACGGATACGGGTTCCTCAAATCGTCCACTTCAGAGCGTTCCGTAAACCTAAAACCCCCGTCCTTAAAg                                      >  2:4370531/1‑140 (MQ=255)

                                                                                                 aaCCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAAccc                                 >  1:1530842/1‑140 (MQ=255)

                                                                                                  aCCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCa                                >  1:1103011/1‑140 (MQ=255)

                                                                                                      gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCGACATACGCCAGCTTCAGCAACGGATACGGTTTCCCCAAACCGTCCACCTCAGAGCACCCCGTAACCTTAAAACCCACCTTCGTATAGAACCCAACCg                            >  2:4153148/1‑140 (MQ=255)

                                                                                                      gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTaa                                                       >  1:1690063/1‑113 (MQ=255)

                                                                                                      gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCg                            >  1:4186124/1‑140 (MQ=255)

                                                                                                      gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCg                            >  1:2234101/1‑140 (MQ=255)

                                                                                                      gAAGTTCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCg                            >  1:1792626/1‑140 (MQ=255)

                                                                                                           tCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGc                       <  1:4949788/140‑1 (MQ=255)

                                                                                                           tCAGCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGc                       <  1:2350487/140‑1 (MQ=255)

                                                                                                              gCCCACCGGGATGAGAAAAAATCGCCTCCGCCCCCACATCCGCCAGATTCAGCACCGGATACCGTTTCCCCCAACCGTCCACCCCAGAGCGTCCCGTAACCCTAAAACCCACCTTCCTATAGAACCCAACCGCCTGCTCa                    >  2:3761018/1‑140 (MQ=255)

                                                                                                              gCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCaaa                                                                                      <  2:3404823/74‑1 (MQ=255)

                                                                                                              gCCCACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCa                                <  2:112450/128‑1 (MQ=255)

                                                                                                                 cACCGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCAttt                 >  2:3130784/1‑140 (MQ=255)

                                                                                                                   ccGGGATGAGAAAAAATCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTg               <  1:4353433/140‑1 (MQ=255)

                                                                                                                             aaaaaaTCGCCTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACg     <  1:3690206/140‑1 (MQ=255)

CCAGTTCCACATCCATGCGCAATCAGCGGTACTCAGTGATAGTGCGGTCATGGCAATGCTTAAGCAGAAATAATCGTGTCACCATTGGTGGGTACTAAACCTGAAGTTCAGCCCACCGGGATGAGAAAAAATCGCTTACGCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATCGTCCACCTCAGAGCGTCCCGTAACCTTAAAACCCACCTTCTTATAGAACCCAACCGCCTGCTCATTTTGCTCATTAACGTTG  >  NZ_CP025268/4215467‑4215734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`