BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,426,442	T→C	pseudogene (275/275 nt)	yjfY ←	DUF1471 family protein YjfY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,426,442	0	T	C	100.0%	892.6 / NA	272	pseudogene (275/275 nt)	yjfY	DUF1471 family protein YjfY
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (105/167); total (105/167)

Only 100 of 275 total aligned reads displayed.

CAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCG‑TG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCG  >  NZ_CP025268/4426304‑4426576

ccgtaGTAGAACTCGCCCTGCAAAAATTTCCTCCCTCCAGACCTACCTGGTCTAATTCCGCAGCCTTTTTTC‑AAACCCCCGCCCCCTCCGACGGGGGCAAAACAACCCATCTCAATCTATCGTTGGTTACACCTGGTCtt                                                                                                                                         <  2:2394762/136‑1 (MQ=255)

  gAAGTAGACCACGCCACGCGGAAATTTCCTTCCTCGGGAGATCACTGGTCTAATTCCGCAGCCGTTTTTC‑CAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAg                                                                                                                                       <  2:3978664/140‑1 (MQ=255)

   aaGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGc                                                                                                                                      >  2:4633925/1‑140 (MQ=255)

   aaGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGc                                                                                                                                      >  2:5177747/1‑140 (MQ=255)

   aaGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGc                                                                                                                                      <  2:2233043/140‑1 (MQ=255)

    aGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCg                                                                                                                                     <  2:507190/140‑1 (MQ=255)

        gaaCACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATaa                                                                                                                                 <  2:1528649/139‑1 (MQ=255)

         aaCACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCTGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATaaa                                                                                                                                >  1:2366072/1‑140 (MQ=255)

         aaCACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATaaa                                                                                                                                >  1:2397971/1‑140 (MQ=255)

             cgcgCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATc                                                                                                                            >  2:281328/1‑140 (MQ=255)

             cgcgCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATc                                                                                                                            >  2:4698863/1‑140 (MQ=255)

             cgcgCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATc                                                                                                                            >  2:3540769/1‑140 (MQ=255)

             cgcgCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATc                                                                                                                            >  2:3127319/1‑140 (MQ=255)

              gcgcTGCGGAAATGTCCTTCCTCTGTAGATAACTGGTCTAACTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGGGCTAGCGATAAATATcc                                                                                                                           <  2:1629813/140‑1 (MQ=255)

                gcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCcg                                                                                                                          >  1:1707262/1‑140 (MQ=255)

                gcTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCcgc                                                                                                                         >  1:1977520/1‑140 (MQ=255)

                gcTGCGGAAATTTCCTTCCTCGGGAGAGAACTGGTCTAATTCCGGAGCCGGTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGAGATAAATATCcgc                                                                                                                         >  2:4737082/1‑140 (MQ=255)

                 cTGTGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCAGTTTTTC‑AAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCcgcg                                                                                                                        <  1:5002598/140‑1 (MQ=255)

                 cTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCcgcg                                                                                                                        <  2:2893761/140‑1 (MQ=255)

                 cTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCcgcg                                                                                                                        <  1:1159906/140‑1 (MQ=255)

                  tGCGGAAATTTCCTTCCTCGGGAGACAACTGGTCTAATTCCGCAGCCGTTTGTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCgcgc                                                                                                                       <  1:4742938/140‑1 (MQ=255)

                   gCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCt                                                                                                                      <  1:4610302/140‑1 (MQ=255)

                    cGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTg                                                                                                                     >  2:770196/1‑140 (MQ=255)

                        aaTTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑t                                                                                                                <  2:306500/140‑1 (MQ=255)

                              cttcctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑Tgcatat                                                                                                          <  1:2747476/140‑6 (MQ=255)

                              cttcctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑Tgcatat                                                                                                          <  1:1247490/140‑6 (MQ=255)

                              cttcctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑Tgcatat                                                                                                          <  1:4936092/140‑6 (MQ=255)

                                 cctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGc                                                                                                       >  1:5155408/1‑140 (MQ=255)

                                    cGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGt                                                                                                    <  1:3733187/140‑1 (MQ=255)

                                     gggAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGtt                                                                                                   >  1:378952/1‑140 (MQ=255)

                                      ggAGCTAACTGGTCTAATGCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTCTACCTGGTGCTAGCGATAACTATCAGCGCTGGCG‑TGCATATTGCCGTTa                                                                                                  <  2:1168087/140‑1 (MQ=255)

                                        agaTAACTGGTCTAATTCCGCAGCCGTTTGTC‑AAAAAAAAGCCCCCTGAGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACt                                                                                                <  1:715817/140‑1 (MQ=255)

                                           tAACTGGTCTAATTCCGCAGACGCTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTCCGTTGGTTATACCTGGGGCTAGCGATAAATTTCTGCGCTGGCG‑TGCATATTCCCTTTACTccc                                                                                             >  2:3253735/1‑140 (MQ=255)

                                           aaaCTGGTAGAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAATTATGGCAATGTTCCGTTGGTTATACCTGGTGCTAGCGATAAATACCCGCGCTGGCG‑TGCATATTGCCGTTACTccc                                                                                             <  1:4358371/139‑1 (MQ=255)

                                            caCTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTTCGAAGGGGGCAAAGCGAGACATGGCAATGTTTCGTTGGTTATAGCTGGTGCTAGGGATAAATATCCGCGCTGTCT‑TGAATTTTGGCGTTGCTccct                                                                                            >  2:1661260/2‑139 (MQ=255)

                                             aCTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCg                                                                                           >  1:3622533/1‑140 (MQ=255)

                                                  tCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTcc                                                                                      <  1:2824183/140‑1 (MQ=255)

                                                  tCTAATGCCGCAGCCGTTTTTC‑AAAAAAGAGCCCGCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTcc                                                                                      <  2:246595/140‑1 (MQ=255)

                                                       ttCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAAGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCAt                                                                                 <  1:351789/140‑1 (MQ=255)

                                                          cGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAg                                                                              <  1:2570737/140‑1 (MQ=255)

                                                            ccgCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                                                            <  1:563496/138‑1 (MQ=255)

                                                            cAGCCGTTTTTG‑AAAAAAAAGCCCCCTGCGAAGGGGTCACAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                                                            >  1:106321/1‑140 (MQ=255)

                                                            cAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGTCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                                                            >  1:1588277/1‑140 (MQ=255)

                                                            cAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAGTATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                                                            >  1:4787837/1‑140 (MQ=255)

                                                            cAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTCCTCCCCGGTTCCCGCATCAGaa                                                                            >  2:3326699/1‑140 (MQ=255)

                                                            cAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                                                            <  2:703620/140‑1 (MQ=255)

                                                            cAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                                                            >  1:3925173/1‑140 (MQ=255)

                                                            cAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                                                            <  1:2328150/140‑1 (MQ=255)

                                                              gCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAAtt                                                                          <  1:789521/140‑1 (MQ=255)

                                                              gCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAAtt                                                                          <  1:1839736/140‑1 (MQ=255)

                                                               ccGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                         <  1:2159156/140‑1 (MQ=255)

                                                               ccGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                         <  2:1297005/140‑1 (MQ=255)

                                                                cGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTAc                                                                        <  1:626289/140‑1 (MQ=255)

                                                                 ttttttC‑AAAAAAACGCCCAATGCGACGGGGGCAAACCAAACTATGGCAATGTTTCGTTTGTTATACCTCGTGCTTGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                       <  2:2773265/139‑1 (MQ=255)

                                                                 gTTTTTC‑AAATTAAAGCGCCTTGCGAAGGGGGCAAAGGACCCTGTGGCGATGTTTCGTTGGTTATGCCTGGTGCTAGCGATGAATTTCCTCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                       <  2:1862540/140‑1 (MQ=255)

                                                                 gTTTTTC‑AAAAAACAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                       <  1:4351952/140‑1 (MQ=255)

                                                                 gTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                       <  1:4693386/140‑1 (MQ=255)

                                                                 gTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                       <  1:2945180/140‑1 (MQ=255)

                                                                 gTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                       <  1:3270091/140‑1 (MQ=255)

                                                                 gTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                       <  1:4039798/140‑1 (MQ=255)

                                                                 gTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                       <  1:682735/140‑1 (MQ=255)

                                                                 gTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGACAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCGGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                       <  1:3096170/140‑1 (MQ=255)

                                                                 gTTATTC‑AAAAAAAAGCCCCCTCCTAAGGGAGCAAAGAAATCTTTGGCAATGTTTTGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTTC‑ATATTGCCGTTACTCCCCGGTTCCCGCCTCAGAATTACg                                                                       <  2:63715/140‑1 (MQ=255)

                                                                   ttttC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTg                                                                     <  2:4857918/140‑1 (MQ=255)

                                                                      tC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTAGACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGgta                                                                  <  1:4187614/140‑1 (MQ=255)

                                                                      tC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTCCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGgta                                                                  <  2:4379855/140‑1 (MQ=255)

                                                                         aaaaaaaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGgtagt                                                                <  2:3994037/140‑1 (MQ=255)

                                                                                   ccccTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGcc                                                      <  1:4240941/140‑1 (MQ=255)

                                                                                           aaGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCAt                                              <  1:316056/140‑1 (MQ=255)

                                                                                              ggggCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTg                                           >  2:4702455/1‑140 (MQ=255)

                                                                                              ggggCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTg                                           <  2:881554/140‑1 (MQ=255)

                                                                                                ggCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGtt                                         >  1:3460642/1‑140 (MQ=255)

                                                                                                ggCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGtt                                         >  1:807166/1‑140 (MQ=255)

                                                                                                ggCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCGGtt                                         >  2:4297423/1‑140 (MQ=255)

                                                                                                 gcaaagcaaaCTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGttt                                        >  2:2659803/1‑140 (MQ=255)

                                                                                                 gcaaagcaaaCTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGttt                                        >  2:105549/1‑140 (MQ=255)

                                                                                                  caaagcaaaCTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTttc                                       >  1:1359912/1‑140 (MQ=255)

                                                                                                  caaagcaaaCTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTttc                                       <  2:535228/140‑1 (MQ=255)

                                                                                                   aaagcaaaCTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTttct                                      <  1:100254/140‑1 (MQ=255)

                                                                                                         aaCTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCAttt                                <  2:1981961/140‑1 (MQ=255)

                                                                                                         aaCTATGGCAATGCTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCAttt                                <  2:4517811/140‑1 (MQ=255)

                                                                                                               ggCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGcc                          >  2:3759168/1‑140 (MQ=255)

                                                                                                               ggCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGcc                          >  1:2221946/1‑140 (MQ=255)

                                                                                                               ggCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGcc                          >  1:3355750/1‑140 (MQ=255)

                                                                                                               ggCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGcc                          >  1:4007773/1‑140 (MQ=255)

                                                                                                               ggCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGGGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGcc                          >  2:4136847/1‑140 (MQ=255)

                                                                                                                 cAATGTTTCGTTGGTTATCCCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTTCCCGGTTCCCGCATCCGAAGTACGTGGTAGTACGTTGCGCCTTGCGCATCTCTTTCTTCATTTAATGCCTg                        <  2:175703/140‑1 (MQ=255)

                                                                                                                    tGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACg                     >  2:2745674/1‑140 (MQ=255)

                                                                                                                      tttCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTg                   >  2:3507690/1‑140 (MQ=255)

                                                                                                                      tttCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTg                   >  1:406816/1‑140 (MQ=255)

                                                                                                                      tttCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTg                   >  1:2780920/1‑140 (MQ=255)

                                                                                                                         cGTTGGTTATGCCTGGTGCTAGCGATAAATGTCGGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGGAGTACGTTGCGCCTTGCGCATCTGGTTCTTCATTTAATGCCTGACGTgatg                <  2:4143603/140‑4 (MQ=255)

                                                                                                                              gTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCtt           >  2:5042858/1‑140 (MQ=255)

                                                                                                                                tataCCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTc         >  2:1934247/1‑140 (MQ=255)

                                                                                                                                      tGGTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGc   <  1:939279/140‑1 (MQ=255)

                                                                                                                                      tGGTGCTAGCGATAAATAGCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACATTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGc   <  2:3711141/140‑1 (MQ=255)

                                                                                                                                       ggTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCg  <  1:3819211/140‑1 (MQ=255)

                                                                                                                                       ggTGCTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCAGACGTGCTTCGCTTTCAGTGGCg  <  2:1894605/140‑1 (MQ=255)

                                                                                                                                        gtgcTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTcc                                                                                      <  1:2340247/55‑1 (MQ=255)

                                                                                                                                        gtgcTAGCGATAAATATCCGCGCTGGCG‑TGCATATTGCCGTTACTCCCCGGTTcc                                                                                      >  2:2340247/1‑55 (MQ=255)

CAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTC‑AAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCG‑TG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCG  >  NZ_CP025268/4426304‑4426576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`