BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,537,356	T→C	pseudogene (651/1241 nt)	yjhR →	helicase YjhR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,537,356	0	T	C	99.4%	1095.2 / NA	332	pseudogene (651/1241 nt)	yjhR	helicase YjhR
Reads supporting (aligned to +/- strand): ref base T (1/0); major base C (158/171); minor base A (0/2); total (159/173)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 332 total aligned reads displayed.

TCTCTTTTCTCCTGTCTACTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCATGGTGTGGAG  >  NZ_CP025268/4537220‑4537490

 ctctTTTCTCCTGTCTACTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGc                                                                                                                                    >  1:3546430/1‑140 (MQ=255)

 ctctTTTCTCCTGTCTACTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGc                                                                                                                                    >  2:3485647/1‑140 (MQ=255)

    ttttCTCCTGTCTACTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCAtt                                                                                                                                 >  1:4530816/1‑140 (MQ=255)

      ttCTCCTGTCTACTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCAtttt                                                                                                                               >  1:1916445/1‑140 (MQ=255)

         tcCTGTCTACTCAAAACACGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTcgc                                                                                                                            >  1:3951312/1‑140 (MQ=255)

             gTCTACTCAAAACATGAAGATGGCAGGTTTCTGGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGAACCTTATCGAGATGCAGCCCGCATTTTcgccgct                                                                                                                        >  2:2410512/1‑139 (MQ=255)

              tCTACTCAAAACATGAAGATGGCAGGTTTCTTGATCGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGa                                                                                                                       <  2:2777439/140‑1 (MQ=255)

              tCTACTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGa                                                                                                                       <  1:849772/140‑1 (MQ=255)

              tCTACTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGa                                                                                                                       <  1:4908356/140‑1 (MQ=255)

                tACTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAgg                                                                                                                     >  1:1658567/1‑140 (MQ=255)

                tACTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAgg                                                                                                                     >  2:3751244/1‑140 (MQ=255)

                      aaaCATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTAtt                                                                                                               >  1:1592315/1‑140 (MQ=255)

                        aCATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTgg                                                                                                             <  1:4265992/140‑1 (MQ=255)

                        aCATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTgg                                                                                                             <  2:2192238/140‑1 (MQ=255)

                          aTGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCa                                                                                                           >  1:3692132/1‑140 (MQ=255)

                            gAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCaaa                                                                                                         >  2:538183/1‑140 (MQ=255)

                            gAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCaaa                                                                                                         >  1:3065212/1‑140 (MQ=255)

                                aTGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTCGCAAAatat                                                                                                     <  1:286501/140‑1 (MQ=255)

                                       gTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCtttt                                                                                              <  2:2138609/140‑1 (MQ=255)

                                         ttCTTGATAGCAACAGCACTATCCTCAATGTTACTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCGCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTttct                                                                                            <  1:3550507/140‑1 (MQ=255)

                                            ttGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCa                                                                                         <  1:3337255/140‑1 (MQ=255)

                                            ttGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCACTAAAGATCGTTTTCTGGTATTTGGCGATATGGACCTTATCTAGATGCAGCTCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCa                                                                                         <  1:4213019/140‑1 (MQ=255)

                                            ttGATAGCAACAGCAACATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCa                                                                                         <  2:2715527/140‑1 (MQ=255)

                                              gATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGa                                                                                       <  1:4613491/140‑1 (MQ=255)

                                                 aGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGAGAGTTTTTTGGTATTGGGCGAGATGGTCCTTATCGAGAGGCAGTCCGCATTTTCGCCGCTAGGGTGATTGGCAAAATATATCTTTTCTTCAGataa                                                                                    >  2:346763/1‑140 (MQ=255)

                                                  gCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGataat                                                                                   >  1:3896215/1‑140 (MQ=255)

                                                      cAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATg                                                                               >  1:197424/1‑140 (MQ=255)

                                                      cAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATg                                                                               <  1:4633664/140‑1 (MQ=255)

                                                              tCCTCAATGTTGCTGTCTCACGCGCTAAAGGTAGTTTGCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCaa                                                                       >  1:2292065/1‑140 (MQ=255)

                                                              tCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCaa                                                                       >  1:1468006/1‑140 (MQ=255)

                                                              tCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCaa                                                                       >  2:1254611/1‑140 (MQ=255)

                                                              tCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCaa                                                                       >  1:2121507/1‑140 (MQ=255)

                                                              tCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCaa                                                                       >  1:4721769/1‑140 (MQ=255)

                                                              tCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCaa                                                                       >  1:3432426/1‑140 (MQ=255)

                                                              tCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCaa                                                                       >  1:3314428/1‑140 (MQ=255)

                                                              tCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCaa                                                                       >  1:2628304/1‑140 (MQ=255)

                                                              tCCTCAATGTTGCGGTCTCACTCGTTAAAGCTAGTTTTCTGGTATTTGGCGATATGGACAATATCGACATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAACTATCTTTTTTCTTCAGATAATAATGCTTTTCaa                                                                       >  2:3220953/1‑140 (MQ=255)

                                                               ccTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAAt                                                                      >  1:1816246/1‑140 (MQ=255)

                                                                   aaTGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGa                                                                  >  1:1279619/1‑140 (MQ=255)

                                                                   aaTGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGa                                                                  >  1:1770042/1‑140 (MQ=255)

                                                                   aaTGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGa                                                                  >  1:1531883/1‑140 (MQ=255)

                                                                   aaTGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGATATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGa                                                                  >  2:4160811/1‑140 (MQ=255)

                                                                   aaTGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGATATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATAGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGa                                                                  >  2:4494671/1‑140 (MQ=255)

                                                                        tGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAGAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGtttt                                                             >  1:4963054/1‑139 (MQ=255)

                                                                        tGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTc                                                             >  2:3554296/1‑140 (MQ=255)

                                                                          cTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTCTCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAg                                                           >  2:4645767/1‑140 (MQ=255)

                                                                          cTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAg                                                           >  1:2721220/1‑140 (MQ=255)

                                                                             tctcACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGaaa                                                        >  2:2220166/1‑140 (MQ=255)

                                                                             tctcACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGCTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGaaa                                                        >  1:4144979/1‑140 (MQ=255)

                                                                               tcACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACg                                                      >  1:2980737/1‑140 (MQ=255)

                                                                               tcACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACg                                                      >  1:3284808/1‑140 (MQ=255)

                                                                                cACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGa                                                     <  1:2529923/140‑1 (MQ=255)

                                                                                   gcgcTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAg                                                  <  2:4870790/140‑1 (MQ=255)

                                                                                       tAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCTAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGa                                                     <  2:3117385/133‑1 (MQ=255)

                                                                                       tAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGAtt                                              >  2:3863511/1‑140 (MQ=255)

                                                                                        aaaGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGAttt                                             <  1:442316/140‑1 (MQ=255)

                                                                                              aGTTTTCTGGTATTTGGCGATATGGACGTAATCGAGAAACAGACCGCATTTTCGCAGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTc                                       <  2:1379060/140‑1 (MQ=255)

                                                                                               gTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTcc                                      >  2:3302374/1‑140 (MQ=255)

                                                                                                 tttCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGc                                    >  2:3410757/1‑140 (MQ=255)

                                                                                                   tCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGcac                                  >  1:4491801/1‑140 (MQ=255)

                                                                                                     tGGTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACAt                                >  2:3935773/1‑140 (MQ=255)

                                                                                                       gTATTTGGCGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATac                              <  2:730454/140‑1 (MQ=255)

                                                                                                          tttGGCGATTTGGTCCTTAGCGAGTTGCCGCTCGCATTTTCGCCTCGTGGGGTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCATAAACGACATGATTTAATGTCCGCACTTAcaca                           <  2:3433925/140‑1 (MQ=255)

                                                                                                               cGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAAttt                      <  1:5087064/140‑1 (MQ=255)

                                                                                                               cGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAAttt                      <  1:2358028/140‑1 (MQ=255)

                                                                                                               cGATATGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAAttt                      <  2:4779205/140‑1 (MQ=255)

                                                                                                               cGATACGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAAttt                      <  2:4575082/140‑1 (MQ=255)

                                                                                                                 atatGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGCATTTAATTCCCGCACTTACACAAATTTcc                    <  2:1031240/140‑2 (MQ=255)

                                                                                                                    tGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACg                 <  1:769343/140‑1 (MQ=255)

                                                                                                                    tGGACCTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACg                 <  1:738164/140‑1 (MQ=255)

                                                                                                                         cTTATCGAGATGCAGCCCGCATTTTCGCCGCGTGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa            <  2:4320174/140‑1 (MQ=255)

                                                                                                                         cTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa            <  1:619007/140‑1 (MQ=255)

                                                                                                                         cTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa            <  1:3815337/140‑1 (MQ=255)

                                                                                                                         cTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa            <  1:1109618/140‑1 (MQ=255)

                                                                                                                         cTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa            <  1:1219099/140‑1 (MQ=255)

                                                                                                                         cTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa            <  1:2144247/140‑1 (MQ=255)

                                                                                                                         cTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa            <  1:2461090/140‑1 (MQ=255)

                                                                                                                         cTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa            <  1:2953671/140‑1 (MQ=255)

                                                                                                                         cTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa            <  1:3752744/140‑1 (MQ=255)

                                                                                                                         cTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa            <  2:2664592/140‑1 (MQ=255)

                                                                                                                         cTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa            <  1:5183189/140‑1 (MQ=255)

                                                                                                                         cTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa            <  2:2628304/140‑1 (MQ=255)

                                                                                                                         cTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa            <  2:2354388/140‑1 (MQ=255)

                                                                                                                         cTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa            <  2:1683863/140‑1 (MQ=255)

                                                                                                                         cTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa            <  2:1134613/140‑1 (MQ=255)

                                                                                                                         cTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACAGACACAAATTTCAACGCTGCa            <  2:5194904/140‑1 (MQ=255)

                                                                                                                         cTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGGTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa            <  1:5129227/140‑1 (MQ=255)

                                                                                                                         cTTATCGAGATGCAGCCCGCATTTTCGCCGCGAGAGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa            <  2:2574535/140‑1 (MQ=255)

                                                                                                                         cTTATCGAGATGCAGCCCGCAGTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTGCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa            <  2:2076696/140‑1 (MQ=255)

                                                                                                                         cTTATCGAGATGCAGCCAGCATTTTTGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCa            <  2:4711028/140‑1 (MQ=255)

                                                                                                                         cTTATCGACATGCAGCCCGCCTTTTCGCCGCGAGTGTTATTGGCAAAATCTCTCTTTTCTTCAGATAATCATGCGTTGCAAGTTGAGTTTCACAAACGCCAGGCTTTAATTTCCGTACATACACACAGTTCAACGCTGCa            <  2:1595376/140‑1 (MQ=255)

                                                                                                                           tATCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCATg          >  2:4603509/1‑140 (MQ=255)

                                                                                                                            aTCGAGGTGCAGCCCGCATTTTCGCCGCGAGGGCTATTGTCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTAGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAGAGTTCAACTCGGCATgg         >  2:571184/1‑140 (MQ=255)

                                                                                                                            aTCGAGATGCAGCTCGCATTTTCGCCGCGAGGGGTATTGGCAAAATATCTTTTTTCTTCAGATAATAATGCGGTTCAATTTTATTTTCAGAAACGACAGGATTTGTTTTGCGCTCTTACACAAATATCAACGCTGCATgg         >  2:1969950/1‑140 (MQ=255)

                                                                                                                            aTCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCATgg         >  1:2087279/1‑140 (MQ=255)

                                                                                                                            aTCGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCATgg         >  2:5023205/1‑140 (MQ=255)

                                                                                                                            aTCGAGATGCAGCCCGCATTTTCGCAGCGAGGGTTATTGGCAAAATATCTCTTTTCATCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGATAGGAATTAATTTCAGCACATACAAAAATTTCCACGCGGCAAgg         >  2:4957411/1‑140 (MQ=255)

                                                                                                                              cGAGATGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCATGgtg       >  2:3983893/1‑140 (MQ=255)

                                                                                                                                 gaTGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCATGGTGTgg    >  2:1515708/1‑140 (MQ=255)

                                                                                                                                  aTGCAGCCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGTGTTTCAGAAACGACAGGTTTTAATTTCCGCACATACACAAATTTCAACGCTGCATGGTGTGGa   >  1:1092719/1‑140 (MQ=255)

TCTCTTTTCTCCTGTCTACTCAAAACATGAAGATGGCAGGTTTCTTGATAGCAACAGCACTATCCTCAATGTTGCTGTCTCACGCGCTAAAGATAGTTTTCTGGTATTTGGCGATATGGACCTTATCGAGATGCAGTCCGCATTTTCGCCGCGAGGGTTATTGGCAAAATATCTCTTTTCTTCAGATAATAATGCGTTGCAATTTGAGTTTCAGAAACGACAGGATTTAATTTCCGCACATACACAAATTTCAACGCTGCATGGTGTGGAG  >  NZ_CP025268/4537220‑4537490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`