Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 4,608,154 G→A noncoding (48/87 nt) CXP41_RS23870 ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252684,608,1540GA97.8% 747.6 / ‑5.7 232noncoding (48/87 nt)CXP41_RS23870tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base A (110/115);  total (114/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 232 total aligned reads displayed.

CCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAG  >  NZ_CP025268/4608020‑4608289
                                                                                                                                       |                                                                                                                                       
ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                    <  1:3652684/140‑1 (MQ=255)
ccACGGCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTAGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                    <  2:2480599/140‑1 (MQ=255)
 cccGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATGTCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTa                                                                                                                                   <  2:641825/138‑1 (MQ=255)
 cACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTa                                                                                                                                   >  1:2261335/1‑140 (MQ=255)
      ccGTAAGCACCCGAACACCTGAAGATAGAGTGTCTACTAATTCCGCCACCTTAACACAGTCATCTTACTTTTTTTGATATCGCCTTATTTGGTGCGA‑GGGGGGGCACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                                                              <  2:4959963/140‑1 (MQ=25)
        gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCACCGTTCTTTTTTGGAGATGGCGCCGTTTGGTGCGA‑TGGGGGGGACGTGAACCCCCACGTCCGGACGAAGGCTAacacggg                                                                                                                            >  2:4078991/1‑137 (MQ=18)
         tAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGa                                                                                                                           >  1:4509118/1‑140 (MQ=255)
              acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTCTTTTTGATATCGCTTCGTTTGGAGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                                                                                      >  2:512461/1‑140 (MQ=255)
                acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGCACCCCCACGTCCGTAAGAACACTAACACCTTAAGCTAgc                                                                                                                    >  1:542130/1‑140 (MQ=255)
                acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                                                                                                    >  1:4282060/1‑140 (MQ=255)
                  tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCTCAATACTTTTATTGATTTCCTCCCGTTTAGGGGGA‑GGCGAGGGGCTTTAACCTCCTCGTCAGTAAGAACACAACCACCGGGAGCTAgcgc                                                                                                                  >  2:3686354/1‑140 (MQ=12)
                  tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcgc                                                                                                                  >  1:2993013/1‑140 (MQ=255)
                  tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTAAAGCTAgcgc                                                                                                                  >  1:3971758/1‑140 (MQ=255)
                  tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTATTGATATCGCCTCGTTTGGGGCGA‑GGGGGGGGGCTTGAACCTCCACGTCAGTAAGAAAACTAACACCTGAGGCTAgcgc                                                                                                                  >  2:3622936/1‑140 (MQ=37)
                  tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATATTACTTATATTGATATCGCCCCGTTTGGTTCGA‑GGCATGGGACTTGAACCACCACGACGGTAATAACACTCACACCTGACGCTAgcgc                                                                                                                  >  2:269112/1‑140 (MQ=21)
                  tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCACCTTACTTTTTTTGATATCGCCTCGTTTGGTCCGA‑CGGCGGGGACTTGAACCCCCACGTCCGTAAGAACACTAAGACCTGAAGCTAgcgc                                                                                                                  >  1:3746445/1‑140 (MQ=37)
                          gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTAGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACCCTATCCCCTGAAGCTAGCGCGGCTAccg                                                                                                          >  2:4305475/1‑139 (MQ=37)
                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGCACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                                    >  1:464579/1‑140 (MQ=255)
                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                                    >  1:94586/1‑140 (MQ=255)
                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                                    >  1:1251154/1‑140 (MQ=255)
                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                                    >  1:1674688/1‑140 (MQ=255)
                                 gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                                                   <  1:777091/140‑1 (MQ=255)
                                 gcgcGGCTACCAAATCCGCCACCTTCCCACAGTCAGCTCACTAAGATCGATCTCGCCACGTTTGGTGCGA‑GGGGGGGGACTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCCATTCCGc                                                                                                   <  2:2323958/140‑1 (MQ=17)
                                 gcgaGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATCTCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATACCGc                                                                                                   <  1:4411132/140‑1 (MQ=255)
                                  cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATgccgcc                                                                                                  >  2:4885744/1‑140 (MQ=255)
                                  cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                                                                  >  2:5056616/1‑140 (MQ=255)
                                     gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                                                               <  1:1560947/140‑1 (MQ=255)
                                     gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                                                               >  1:4137072/1‑140 (MQ=255)
                                     gTCTACCAATTCCGCCACCTTCGCACAGTCACCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                                                               <  1:253153/140‑1 (MQ=255)
                                      tCTACCAATTCCGGCACCTTCGCACAGTGAACTTACTTTTTTTGATATCACCGCGTTTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                                                               <  2:1745539/140‑1 (MQ=18)
                                      tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                                                                                              >  1:585183/1‑140 (MQ=255)
                                           cgaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTGGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                         <  2:2389004/138‑1 (MQ=255)
                                           cAATTCCGCGACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                         <  2:319186/140‑1 (MQ=255)
                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                         <  2:585183/140‑1 (MQ=255)
                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                         <  1:2390364/140‑1 (MQ=255)
                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTGGATATCACCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                         <  2:1137794/140‑1 (MQ=255)
                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTGTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                         <  2:2861452/140‑1 (MQ=255)
                                           cAATTCCGCCACCAACACACAGTGAACTTACTTTTATTGATATCGCCACGATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                         <  2:1710895/140‑1 (MQ=17)
                                               tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt                                                                                     >  1:4962323/1‑140 (MQ=255)
                                               tCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt                                                                                     >  1:4965924/1‑140 (MQ=255)
                                                ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                                                    <  1:176115/140‑1 (MQ=255)
                                                ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                                                    <  2:3527367/140‑1 (MQ=255)
                                                ccGCCACCTTAGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                                                    <  2:2007000/140‑1 (MQ=255)
                                                ccGCCACCCACGCGCAGGCATGTTACTTTGTTAGCAATCGCATCGTTTGGTGCGA‑GGGGGGGGACTTGAGCCCCCCCGTCCGTAAGAACACTAACACCTGACGCTTGCGCGTCTACCAATGCCGCCACCTTCGCCCAGTg                                                                                    <  2:4461216/140‑1 (MQ=14)
                                                 cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc                                                                                   >  1:1484277/1‑140 (MQ=255)
                                                   ccACCTTCGCACAGTCATCTGACTTTTTTTGATATCGCCTCGTTTGGTGCGG‑GGGGTGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa                                                                                 >  2:3160069/1‑140 (MQ=255)
                                                    cACCTTCGCACAGTCATCTTACTTTTTTTGTGCTCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                <  1:4345147/140‑1 (MQ=255)
                                                    cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                <  1:2842161/140‑1 (MQ=255)
                                                    cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                <  1:1197597/140‑1 (MQ=255)
                                                         tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAata                                                                           >  2:4000570/1‑140 (MQ=255)
                                                          cGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAatat                                                                          <  1:3029008/140‑1 (MQ=255)
                                                              caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                                                      <  1:735661/140‑1 (MQ=255)
                                                              caGGCATCTTACTTGTTTGGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                                                      <  2:3894784/140‑1 (MQ=255)
                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                                                  <  1:1065461/77‑1 (MQ=255)
                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTg                                                                   >  1:1976756/1‑140 (MQ=255)
                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTg                                                                   >  1:1930676/1‑140 (MQ=255)
                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTg                                                                   >  2:5159677/1‑140 (MQ=255)
                                                                    tCTTACTTTTTTTAATATCGCCTCGTTTGGTGCGA‑GGGGGTGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGCGCTAGCAATATCAACGTGGtt                                                                >  2:4113718/1‑140 (MQ=18)
                                                                      ttACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTAGtttt                                                              >  2:4578194/1‑140 (MQ=255)
                                                                         cTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTgg                                                           >  1:2121462/1‑140 (MQ=255)
                                                                           ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                                                               <  2:2708806/102‑1 (MQ=255)
                                                                           ttttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACGAACACCTGAAGCTAGCGCGTCTCCCAATTCCGCCACCTTCGCCCAGTGCGGGCAAGATCAACGTGGTTTTTGGTg                                                         <  2:5212617/140‑1 (MQ=21)
                                                                             ttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCGGGAAGGACACTAACACCTGGAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCGCAGGGCGATCGCTAGCAACGTAGTGTTTtgtggg                                                       >  2:1346747/1‑138 (MQ=17)
                                                                              tttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                                                      <  2:2213777/140‑1 (MQ=255)
                                                                              tttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                                                      >  2:2910072/1‑140 (MQ=255)
                                                                                   tatCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGGCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                                                 <  1:5015746/140‑1 (MQ=255)
                                                                                   tatCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                                                 <  1:1543800/140‑1 (MQ=255)
                                                                                     tCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCACACGTCCGTAAGAACAATCACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggggg                                               <  2:1771337/140‑1 (MQ=255)
                                                                                        ccTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGAc                                            >  2:3331038/1‑140 (MQ=255)
                                                                                        ccTCGTTGGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGAc                                            <  2:1835253/140‑1 (MQ=255)
                                                                                           cGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTg                                         >  2:3427901/1‑140 (MQ=255)
                                                                                             tttGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGaa                                       >  1:1636693/1‑140 (MQ=255)
                                                                                                 gTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAcccc                                   >  1:3866607/1‑140 (MQ=255)
                                                                                                   gggA‑GGGGAGAGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGGGCGTCTACCAATACCGCCACCTTCGCACAGTGCGCGCAATATCAACGTGGTTTTTCGTGCGAGGGGGGGGACTTGAACCCCCa                                 <  2:3818322/138‑1 (MQ=21)
                                                                                                   gCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCa                                 <  1:4712676/140‑1 (MQ=255)
                                                                                                   gCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCa                                 <  1:1804592/140‑1 (MQ=255)
                                                                                                   gCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCa                                 <  1:4736511/140‑1 (MQ=255)
                                                                                                   gCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCa                                 <  1:1251879/140‑1 (MQ=255)
                                                                                                   gCGA‑GGGGGGGGACTGGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTACGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCa                                 <  2:2200476/140‑1 (MQ=255)
                                                                                                      a‑GGGGGGGGACTTGAACCCCCACGTCCGTAACAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGGCCAGTGAGACAAATATCAAAGTGTATTTTGGTGCGAGGGGGGGAGCTCGTACTCCCACGt                              >  1:1009384/1‑140 (MQ=17)
                                                                                                        ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTc                             >  1:2179734/1‑140 (MQ=255)
                                                                                                          ggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCg                           <  1:1317648/140‑1 (MQ=255)
                                                                                                          ggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCg                           <  1:2076573/140‑1 (MQ=255)
                                                                                                             gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTaa                        >  1:4192419/1‑140 (MQ=255)
                                                                                                             gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGGGTTTTTTGGTGCGAGGGGGGGGGCTTGAACCCCCACGTCCGTaa                        >  1:768975/1‑140 (MQ=255)
                                                                                                               gACTTGAACCCCCACGTCCGTAAGACCACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAgg                      <  1:3161258/140‑1 (MQ=255)
                                                                                                               gACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAgg                      <  1:690927/140‑1 (MQ=255)
                                                                                                               gACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAgg                      <  1:3427901/140‑1 (MQ=255)
                                                                                                               caCTGGACCCCCCACGTCCGTAAGGACACAAACCCCTGAAGCTGGCGCGTCTACCAAGACGGCAACCTTAGCAAAGTGCGAGCAATGTAAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAgg                      <  2:1632282/139‑1 (MQ=25)
                                                                                                                aCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGCTTGAACCCCCACGTCCGTAAGGa                     <  2:751593/140‑1 (MQ=255)
                                                                                                                aCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGa                     >  2:1521997/1‑140 (MQ=255)
                                                                                                                aCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGa                     <  1:512658/140‑1 (MQ=255)
                                                                                                                 cTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGac                    >  1:276799/1‑140 (MQ=255)
                                                                                                                    gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                 <  1:3363413/140‑1 (MQ=255)
                                                                                                                    gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                 <  1:52485/140‑1 (MQ=255)
                                                                                                                         cccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAacac            <  1:4582965/140‑1 (MQ=255)
                                                                                                                          ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGCCTTGAACCCCCACGTCCGTGAGGACACTAACAcc           >  1:2678900/1‑140 (MQ=255)
                                                                                                                                ccGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGc     >  2:1648991/1‑140 (MQ=255)
                                                                                                                                   tAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg  <  1:5063963/140‑1 (MQ=255)
                                                                                                                                   tAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg  >  1:1107563/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                       
CCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAG  >  NZ_CP025268/4608020‑4608289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑