BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,621,820	G→A	G104E (GGA→GAA)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,621,820	0	G	A	99.0%	1110.4 / NA	308	G104E (GGA→GAA)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand): ref base G (3/0); new base A (156/147); total (159/149)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 309 total aligned reads displayed.

TCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGG  >  NZ_CP025268/4621683‑4621955

 cTGGGGCTGGCGAAAGCACACGAAGGATCTACAGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAAAGTCATCGGGTACAGATACCCCGTCTGGTCACTGGGAAAt                                                                                                                                      >  2:512152/1‑140 (MQ=255)

    gggCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCAATGGGAAATTGc                                                                                                                                   <  2:5004064/140‑1 (MQ=255)

      gCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCg                                                                                                                                 <  1:738752/140‑1 (MQ=255)

       cTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCgg                                                                                                                                <  1:921373/140‑1 (MQ=255)

       cTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCgg                                                                                                                                <  1:1687142/140‑1 (MQ=255)

       cTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCgg                                                                                                                                <  1:2326650/140‑1 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGGATGGACGGCAACGCTGAAGTTCTCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGTTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGgtgt                                                                                                                             >  1:1516972/1‑140 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAGATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGgtgt                                                                                                                             >  1:3586914/1‑140 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATGCGGTAAAGATACCCCGTCTGGTCACTGGGAAAGTGCCGgtgt                                                                                                                             >  2:3912888/1‑140 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGgtgt                                                                                                                             >  2:1714755/1‑140 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGgtgt                                                                                                                             >  1:2188784/1‑140 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGgtgt                                                                                                                             >  1:4591675/1‑140 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGgtgt                                                                                                                             >  1:1033824/1‑140 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGAGCGTACGCAGGGGCGCAGGAAAGGTCATCCGGTAAAGAAACCCCGTCTGGTCACGGGTAAATAGCCAgtgg                                                                                                                             >  2:2512951/1‑139 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAAAGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAGAGATACCCCGTCTGGTCACTGGGAAATTGCCGgggt                                                                                                                             >  2:3016424/1‑140 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAAGGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGGCATCCGGTAAAGATACCCCGTCTGGTCACTGGGACATTGCGAgtgt                                                                                                                             >  2:2407364/1‑140 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGGTTCATTCTGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCGTGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGCCAGGTCACGGGGAAATTGCCGgtgt                                                                                                                             >  2:126007/1‑140 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGGTTCATTCCGGCGGGAAGGGAGGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACACCGTCTGGTCAGTGGGAAATTGCGGttgt                                                                                                                             >  2:3961802/1‑140 (MQ=255)

          gCGAAAGCAAAAGAAGTTTCTACCGGTTTCATTCCGGCGGGAATGGGCGGCAACGCAGAAGTTATCGGCGAGGACGCATGGGCGCAAGAAATGTCATCCGGGGAGGATAGCCCGTCTGGTCACTTGGAAATTGCCGgtgt                                                                                                                             >  2:3067079/1‑140 (MQ=255)

           cGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTc                                                                                                                            <  2:157420/140‑1 (MQ=255)

            gAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTcc                                                                                                                           <  1:3868607/140‑1 (MQ=255)

             aaaGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTccc                                                                                                                          <  1:3384346/140‑1 (MQ=255)

             aaaGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTccc                                                                                                                          <  1:530930/140‑1 (MQ=255)

             aaaGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTccc                                                                                                                          <  1:3932597/140‑1 (MQ=255)

               aGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGAGTACGCATGGGAGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCgg                                                                                                                        <  2:400901/140‑1 (MQ=255)

               aGCACACGAAGGTTCTACAGGTTTAATTCCGGAGGGAATGGAAGGCAACGCTGAAGTTATCGGCGAGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCgg                                                                                                                        <  1:3070458/140‑1 (MQ=255)

                 aacatGAAGGTTATACAGATCTCATGCAGGCGGGAATAGACGGAGAAACTGAAGTTATTGCCGAGTAGGAAAGGGCGCACGAAATGTCATCCGCTAAAGATACCCCGTATGGTCACTGGGAAATTGCCGGTGTCACGGtt                                                                                                                      <  2:430125/139‑1 (MQ=255)

                         gggtCTACCGGTTTCATTCCGGCAGGAATGGACGGCAACGCTGACGTTATCGGTGCGTAAGCATGGGCGCACGAAATGTCGTCGGGTAAAGATACCCCGGCTGGGCACTGGGAAATTGCCGGTGTTCCGGTTCTGGTTGa                                                                                                              >  2:2887582/4‑140 (MQ=255)

                             cTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTgg                                                                                                          <  2:2600479/140‑1 (MQ=255)

                             cTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTgg                                                                                                          >  1:3154469/1‑140 (MQ=255)

                             cTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTgg                                                                                                          <  1:3246938/140‑1 (MQ=255)

                             cTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTgg                                                                                                          <  1:2467357/140‑1 (MQ=255)

                              tACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCgg                                                                                                                                >  1:2977500/1‑117 (MQ=255)

                              tACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCgg                                                                                                                        <  1:878639/125‑1 (MQ=255)

                               aCCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTgggg                                                                                                        <  2:1089763/140‑1 (MQ=255)

                                ccGGTTTCTTTCCGGCGGGAATGGACGGCAACGCTGAAGTTGTCTGCGCGTACGCATGGGCGCACGCAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGa                                                                                                       <  1:3737286/140‑1 (MQ=255)

                                    tttCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGatagt                                                                                                   >  2:5166634/1‑138 (MQ=255)

                                    tttCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATAtt                                                                                                   >  1:2847219/1‑140 (MQ=255)

                                        aTTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATTTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTtctc                                                                                               >  1:5022709/1‑140 (MQ=255)

                                          tCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCg                                                                                             >  1:1798618/1‑140 (MQ=255)

                                          tCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCg                                                                                             <  1:5084261/140‑1 (MQ=255)

                                            cggcggGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGAt                                                                                           <  1:4174444/140‑1 (MQ=255)

                                              gggggAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCa                                                                                         >  1:4154857/3‑140 (MQ=255)

                                              gcggGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCa                                                                                         >  1:4231348/1‑140 (MQ=255)

                                              gcggGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCa                                                                                         >  1:5123904/1‑140 (MQ=255)

                                               cggGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCAc                                                                                        <  1:1439290/140‑1 (MQ=255)

                                                   aaTGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGaaa                                                                                    >  1:2608172/1‑140 (MQ=255)

                                                         cGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCt                                                                              <  1:1349760/140‑1 (MQ=255)

                                                          ggCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCtt                                                                             <  1:4920503/140‑1 (MQ=255)

                                                            cAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTcc                                                                           >  1:231941/1‑140 (MQ=255)

                                                               cGCTGAAGTTATCGGCGCGTACGCATGGGCGCCCGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGc                                                                        >  2:3544844/1‑140 (MQ=255)

                                                                    aaGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAagag                                                                   <  1:4705379/140‑1 (MQ=255)

                                                                    aaGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAagag                                                                   <  2:4103671/140‑1 (MQ=255)

                                                                      gTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgct                                                                 <  1:3689971/140‑1 (MQ=255)

                                                                      gTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgct                                                                 <  1:4044247/140‑1 (MQ=255)

                                                                       ttATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCAAGAAAACAGCTTCCCGCAAGAgctg                                                                <  2:3384978/140‑1 (MQ=255)

                                                                         aTCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctgct                                                              >  1:926790/1‑140 (MQ=255)

                                                                         aTCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctgct                                                              >  1:311261/1‑140 (MQ=255)

                                                                          tCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGGTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAAGAGCTTCCCGCAAGAGctgctg                                                             >  2:1909492/1‑140 (MQ=255)

                                                                          tCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGctgctg                                                             >  1:1477702/1‑140 (MQ=255)

                                                                            ggCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGa                                                           <  1:4088146/140‑1 (MQ=255)

                                                                            ggCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGa                                                           <  2:1196150/140‑1 (MQ=255)

                                                                              cgcgTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATa                                                         <  2:1505384/140‑1 (MQ=255)

                                                                              cgcgTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCGGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATa                                                         >  1:2880203/1‑140 (MQ=255)

                                                                                cgTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATaaa                                                       >  2:4391614/1‑140 (MQ=255)

                                                                                        tGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCGGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACGGCTTCCCGCAATAGCTGCTGGATAAACTGGACGa                                               >  2:4072908/1‑140 (MQ=255)

                                                                                        tGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGTTGGATAAACTGGTCGa                                               <  2:4356704/140‑1 (MQ=255)

                                                                                        tGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGa                                               <  1:1271581/140‑1 (MQ=255)

                                                                                          ggCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATGTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGGTAAACGGGTCGAAc                                             >  2:668337/1‑140 (MQ=255)

                                                                                            cgcACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAAcgc                                           >  1:3496248/1‑140 (MQ=255)

                                                                                               aCGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCt                                        >  2:4731710/1‑140 (MQ=255)

                                                                                                    aTGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCt                                   <  2:1889513/140‑1 (MQ=255)

                                                                                                     tGTCATCCGGTAAAGATACCGCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTATGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTg                                  <  2:4509508/140‑1 (MQ=255)

                                                                                                     tGTCATCCGGTAAAGATACCCCGTCTGGTCACTGAGAAATTGCCGGTGTCCCGGTTCTGTTTGACTGGGGATATTTCACCGATCACGAAAACAGCCTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTg                                  <  2:2770016/140‑1 (MQ=255)

                                                                                                         aTCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCgg                              >  1:3373421/1‑140 (MQ=255)

                                                                                                         aTCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCgg                              >  1:207690/1‑140 (MQ=255)

                                                                                                         aTCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCgg                              >  2:3280162/1‑140 (MQ=255)

                                                                                                          tCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCggg                             <  2:917697/140‑1 (MQ=255)

                                                                                                           ccGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGt                            <  2:2162040/140‑1 (MQ=255)

                                                                                                           ccGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGt                            <  1:3651067/140‑1 (MQ=255)

                                                                                                           ccGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTCGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGt                            <  1:4314976/140‑1 (MQ=255)

                                                                                                            cGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGtt                           <  1:2563647/140‑1 (MQ=255)

                                                                                                             ggTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTTGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTa                          >  1:1557810/1‑140 (MQ=255)

                                                                                                             ggTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTa                          >  1:2243623/1‑140 (MQ=255)

                                                                                                             ggTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGGGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAGACTGGTCGAAAGCGCTAATCTGCCGGGTTa                          >  2:785169/1‑140 (MQ=255)

                                                                                                                      aCCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTaa                 >  2:2028829/1‑140 (MQ=255)

                                                                                                                      aCCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTaa                 >  1:634283/1‑140 (MQ=255)

                                                                                                                        cccGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACt               >  2:4818331/1‑140 (MQ=255)

                                                                                                                        cccGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACt               >  1:3962033/1‑140 (MQ=255)

                                                                                                                        cccGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACt               >  2:471082/1‑140 (MQ=255)

                                                                                                                            ttcggTCAATGGGAGATTGCCGGGTTCCCGGTTGTGTTGGAGTGGAGATAGTCCTCCGATCACGAAAACAGCTTCCCCCAAGAGCTGCTGGCTAAACTGGTCAAAAGCGCTCACCTGCCCGGTTACCTCGGTAGCTGTCa           <  2:4584385/137‑1 (MQ=255)

                                                                                                                            tCTGGTCCCTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATATACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCa           <  1:3326495/140‑1 (MQ=255)

                                                                                                                            tCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCa           >  2:2825854/1‑140 (MQ=255)

                                                                                                                            tCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCa           <  2:634283/140‑1 (MQ=255)

                                                                                                                            tCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCa           >  2:3731109/1‑140 (MQ=255)

                                                                                                                            tCTGGTCACTGGCAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATACACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCa           <  1:4438176/140‑1 (MQ=255)

                                                                                                                             cTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGTATACACTGGTCGCCCGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCAc          >  2:2088698/1‑140 (MQ=255)

                                                                                                                               ggTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCActc        >  2:2301692/1‑140 (MQ=255)

                                                                                                                                gTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCActct       >  1:1868353/1‑140 (MQ=255)

                                                                                                                                     tGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCgg  >  2:2635927/1‑140 (MQ=255)

TCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGG  >  NZ_CP025268/4621683‑4621955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`