BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	442,101	T→C	197 (TAA→TAG)	yajL ←	protein deglycase YajL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	442,101	0	T	C	99.6%	894.6 / NA	274	197 (TAA→TAG)	yajL	protein deglycase YajL
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (148/123); total (149/125)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 276 total aligned reads displayed.

TGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGC  >  NZ_CP025268/441964‑442236

  tgAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACactact                                                                                                                                     >  1:3876949/1‑140 (MQ=255)

    aGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCg                                                                                                                                   >  2:639768/1‑140 (MQ=255)

    aGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAAGTTGTGGTTGTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCg                                                                                                                                   >  2:1518485/1‑140 (MQ=255)

        cgGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaat                                                                                                                               <  1:4727495/140‑1 (MQ=255)

        cgGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaat                                                                                                                               <  2:3563273/140‑1 (MQ=255)

        cgGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCCtaat                                                                                                                               <  2:4811110/140‑1 (MQ=255)

        cgGGGTGATGAGCCGTCTGCAGCCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaat                                                                                                                               <  2:146782/140‑1 (MQ=255)

          gggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGtaataa                                                                                                                             >  2:2601021/1‑140 (MQ=255)

              gatgaGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATtat                                                                                                                         >  2:2519156/1‑140 (MQ=255)

                   gCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACA‑CACTCGTAATAATTATAAATcc                                                                                                                   >  1:1872615/1‑140 (MQ=255)

                     cGTCTGCAGGCGCTCTATCTGCGCGCGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGGAATTTGTGGGTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTCCTCGTAATAATTATAAATccc                                                                                                                  >  2:1451133/1‑140 (MQ=255)

                     cGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTCGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACCCTACTCGTAATAATTATAAATccc                                                                                                                  >  2:3457580/1‑140 (MQ=255)

                       tCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTg                                                                                                                >  1:2123106/1‑140 (MQ=255)

                       tCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGGTTGCCGCATCCGACACTACTCGTAATAATTATAAATCTCTg                                                                                                                >  2:4970779/1‑140 (MQ=255)

                       tCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGGTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTg                                                                                                                >  2:2655922/1‑140 (MQ=255)

                       tCTGCAGGCGCGCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTg                                                                                                                >  2:370391/1‑140 (MQ=255)

                          gCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgccg                                                                                                             <  1:5129999/140‑1 (MQ=255)

                            aGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTgccgcc                                                                                                           <  1:3449850/140‑1 (MQ=255)

                              gcgcTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCAt                                                                                                         <  1:1907867/140‑1 (MQ=255)

                                 ttctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTGTTTACGTCGCATCTGGTCAGATGCGACTTTTGCCGCATCCGACACTACTCGTAATAATTATAAATTCCTGCCGCCATCAc                                                                                                      <  2:4384796/139‑1 (MQ=255)

                                 ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCAc                                                                                                      >  1:2425677/1‑140 (MQ=255)

                                 ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCAc                                                                                                      >  2:115091/1‑140 (MQ=255)

                                 ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCAc                                                                                                      >  2:3596441/1‑140 (MQ=255)

                                 ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCAc                                                                                                      >  2:2699339/1‑140 (MQ=255)

                                 ctctATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGGCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCAc                                                                                                      >  1:1026842/1‑140 (MQ=255)

                                    tATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAACCCCTGCCGCCATCACCAg                                                                                                   <  2:2227431/140‑1 (MQ=255)

                                          cgcgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGa                                                                                             >  1:956651/1‑140 (MQ=255)

                                          cgcgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGa                                                                                             >  1:3857341/1‑140 (MQ=255)

                                          cgcgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGa                                                                                             >  2:4409819/1‑140 (MQ=255)

                                          cgcgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGGAATTTGGGGTTTTTACTGCGCATCTGGTCAGATGCGACGTTTACCGCATCCGAAACGACTCGTAATAATTATAAATCCCTGCCGCCATCAGCAGtcttga                                                                                             >  2:2754251/1‑135 (MQ=255)

                                          cgcgAGCAGGGCTTTAACAATGTGAAGGTATATCGACCGTCATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCATTTGTGa                                                                                             >  2:1927091/1‑140 (MQ=255)

                                             gAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGc                                                                                          <  1:454237/140‑1 (MQ=255)

                                             gAGCAGCGCTTGAAAAATGTGAAGGGATGTCGCCTGTAAGTTGTGGTTTGTACGTGGCATCTGGGGAGATGCGACGTTCGCCGCGGCCGGCACTACTGGTGATGATTATAAAGCCCTGCCGCCATCACCAGTTGTGATgg                                                                                          <  2:3989258/140‑2 (MQ=255)

                                              aGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGcc                                                                                         >  1:778147/1‑140 (MQ=255)

                                              aGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCCCCATCACCAGTTGTGATGcc                                                                                         >  2:4334666/1‑140 (MQ=255)

                                                cAGGGCTTCAACAATGTGAAAGTATATGGCCCGCAATTTGTGGTTTTTACGACGCATCTGGTCAGGTGGGACGTTTGCCGAGTGCGGCACTACGCGGACTAAGTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCAc                                                                                       >  2:4260547/1‑140 (MQ=255)

                                                    gCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGTCACTTCa                                                                                   >  2:2628653/1‑140 (MQ=255)

                                                    gCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCa                                                                                   <  1:1765827/140‑1 (MQ=255)

                                                    gCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCa                                                                                   <  1:839641/140‑1 (MQ=255)

                                                             aTGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCtttt                                                                          >  2:2479879/1‑140 (MQ=255)

                                                             aTGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCtttt                                                                          >  1:3659186/1‑140 (MQ=255)

                                                             aTGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCtttt                                                                          >  2:4557158/1‑140 (MQ=255)

                                                             aTGTGAAGGTATATCGCCCGTAATTTGTCGGTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCtttt                                                                          >  2:4107304/1‑140 (MQ=255)

                                                              tgtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTGATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCttttt                                                                         >  2:3816687/1‑140 (MQ=255)

                                                               gtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTc                                                                        >  2:5093174/1‑140 (MQ=255)

                                                               gtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTc                                                                        >  1:3779801/1‑140 (MQ=255)

                                                                 gAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCAc                                                                      <  1:2601021/140‑1 (MQ=255)

                                                                 gAAGGTATATCGCCCGTAATTTGTGGTTTTTAAGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCAc                                                                      <  2:1098545/140‑1 (MQ=255)

                                                                  aaGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACg                                                                     <  1:728711/140‑1 (MQ=255)

                                                                     gTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGGTGTGATGCCACTTCATGGGCTTTTTCACGctc                                                                  >  1:432386/1‑138 (MQ=255)

                                                                        tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCccaac                                                               >  1:4249288/1‑140 (MQ=255)

                                                                         atCGCCCGTAATTTGTGGTTTTTACTTCGCATCTTGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACTAGTTGTGATGCCACTTCATGGGCTTTTTCACGCccaacc                                                              >  2:409306/1‑140 (MQ=255)

                                                                          tCGCCCGCAATTAGTGGTTTTTACGTCGCATCTGGTCAGATGCGCCTTTCGCCGCATCCGACACTACTCGTAATACTTATCAATCCCTACCGCCATCACCACTTGTGATGCCACTTCATGGGCTTTTTCACGCccaacca                                                             >  2:5046827/1‑140 (MQ=255)

                                                                           cGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCccaaccaa                                                            <  2:3430396/140‑1 (MQ=255)

                                                                            gCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCcaaccaac                                                           >  1:4331747/1‑140 (MQ=255)

                                                                             cccGTGAGTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACa                                                          >  2:2086574/1‑140 (MQ=255)

                                                                             cccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCt                                                                             <  2:3258536/121‑1 (MQ=255)

                                                                             cccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCt                                                                             >  1:3258536/1‑121 (MQ=255)

                                                                             cccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACa                                                          >  1:5191655/1‑140 (MQ=255)

                                                                               cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAgg                                                        >  1:2507736/1‑140 (MQ=255)

                                                                               cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAgg                                                        >  1:140512/1‑140 (MQ=255)

                                                                               cGTAATTTGTGGATTTTACGTCGCATCTAGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGACGCCATCACCAGTTGTGATGCCCCTTCATGGGCTTTTTCACGCCCCACCCACAgg                                                        >  2:2063721/1‑140 (MQ=255)

                                                                                gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAGCAGGt                                                       >  2:4877556/1‑140 (MQ=255)

                                                                                gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGt                                                       >  1:707081/1‑140 (MQ=255)

                                                                                          gTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAAtttt                                             <  1:3581289/140‑1 (MQ=255)

                                                                                          gTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGAAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAAtttt                                             <  1:4029620/140‑1 (MQ=255)

                                                                                             tttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCAGCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAg                                          >  2:4579899/1‑140 (MQ=255)

                                                                                              ttACGTCGCATGTGGTCAGATGCTACGTTTGCCGCATCCGACACTACTCGTAATAATGATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATATGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGa                                         <  2:880145/140‑1 (MQ=255)

                                                                                                aCGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATGACTCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATGATTTTCAGAcc                                       <  1:4312805/140‑1 (MQ=255)

                                                                                                 cGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCa                                      <  2:3913826/140‑1 (MQ=255)

                                                                                                 cGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCa                                      <  2:546425/140‑1 (MQ=255)

                                                                                                 cGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCa                                      <  1:2628653/140‑1 (MQ=255)

                                                                                                 cGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCa                                      <  2:1612705/140‑1 (MQ=255)

                                                                                                 cGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCa                                      <  2:1751433/140‑1 (MQ=255)

                                                                                                    cGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACCCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGAGAATTTTCAGACCAAAg                                   >  1:2866532/1‑140 (MQ=255)

                                                                                                    cGCATCTGGCTAGATGCGACGTTTTTCGCTTCCGGCACTACTCGTAAGCAGTATAAGTCCTTGCCGCCGTCACCAGTTGTGTTGCCCCTCCATGGGCTTTTTCCCGCCCAACCACCAGGTCGATAATTTTCAGACCaatg                                   >  2:2351858/1‑138 (MQ=255)

                                                                                                       aTCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCg                                <  1:361878/140‑1 (MQ=255)

                                                                                                        tctcGTCAGATGCGACGTTTGCCGCATCCGACACTAGTCGTAATCCTTTTAACTCCCTGCCGCCGTCACCACTTGTGATGCCACTTCCTGGGCTTTTTCACGCCCAACCAACAGGTCGCTAAGTTTCAGACCAAAGTCGa                               <  2:4727042/140‑1 (MQ=255)

                                                                                                        tCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGa                               <  1:197875/140‑1 (MQ=255)

                                                                                                        tCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGa                               <  2:3876836/140‑1 (MQ=255)

                                                                                                         cTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGCGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGAt                              <  1:1155352/140‑1 (MQ=255)

                                                                                                         cTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAACCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGAt                              <  1:3710481/140‑1 (MQ=255)

                                                                                                           ggTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAg                            <  1:2346389/140‑1 (MQ=255)

                                                                                                            gTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGc                           >  2:31948/1‑140 (MQ=255)

                                                                                                                 aTGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAc                      >  1:2823494/1‑140 (MQ=255)

                                                                                                                  tGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                     <  1:1711924/140‑1 (MQ=255)

                                                                                                                  tGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                     <  2:2171730/140‑1 (MQ=255)

                                                                                                                  tGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                     <  1:291172/140‑1 (MQ=255)

                                                                                                                  tGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                     <  1:3187998/140‑1 (MQ=255)

                                                                                                                  tGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                     <  1:3416419/140‑1 (MQ=255)

                                                                                                                  tGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                     <  1:4617604/140‑1 (MQ=255)

                                                                                                                   gCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAccc                    >  1:3028632/1‑140 (MQ=255)

                                                                                                                      aCGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGc                 >  1:3473474/1‑140 (MQ=255)

                                                                                                                      aCGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGc                 >  1:1312742/1‑140 (MQ=255)

                                                                                                                      aCGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGc                 >  2:2891962/1‑140 (MQ=255)

                                                                                                                           tGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAAtttt                                             >  1:4976931/1‑107 (MQ=255)

                                                                                                                           tGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctg            >  1:1967888/1‑140 (MQ=255)

                                                                                                                             ccGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctggc          >  2:3340392/1‑140 (MQ=255)

                                                                                                                               gCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctggctg        <  2:2009732/140‑1 (MQ=255)

                                                                                                                                   ccGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCa    <  2:2085130/140‑1 (MQ=255)

TGTGAGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGC  >  NZ_CP025268/441964‑442236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`