BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	449,814	T→C	T283A (ACT→GCT)	cyoA ←	cytochrome o ubiquinol oxidase subunit II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	449,814	0	T	C	100.0%	1110.5 / NA	331	T283A (ACT→GCT)	cyoA	cytochrome o ubiquinol oxidase subunit II
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (243/88); total (243/88)

Only 100 of 331 total aligned reads displayed.

TAATTTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGC  >  NZ_CP025268/449677‑449949

tAATTTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCa                                                                                                                                       >  1:3262214/1‑140 (MQ=255)

tAATTTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCa                                                                                                                                       >  1:3329737/1‑140 (MQ=255)

tAATTTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTACGCTGTGCTCTCCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCa                                                                                                                                       >  1:4781792/1‑140 (MQ=255)

   ttttCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATaa                                                                                                                                    <  1:3897072/140‑1 (MQ=255)

                         ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCCTACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCaac                                                                                                              >  2:2999208/1‑139 (MQ=255)

                         ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCaaa                                                                                                              >  1:1206098/1‑140 (MQ=255)

                         ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCaaa                                                                                                              >  2:2725787/1‑140 (MQ=255)

                         ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCaaa                                                                                                              >  1:2724127/1‑140 (MQ=255)

                         ccTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCaaa                                                                                                              >  1:4966331/1‑140 (MQ=255)

                         ccTCAACCCCTTTAATGGGCGGAGTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTTTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCaaa                                                                                                              >  1:2356007/1‑140 (MQ=255)

                               ccccTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGCCAAACAAGTc                                                                                                        >  2:2107258/1‑140 (MQ=255)

                                 ccTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTg                                                                                                      >  1:4106942/1‑140 (MQ=255)

                                  cTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATGAACTTGTTAATTACATCGGCAAACAAGTCTgg                                                                                                     >  2:1590499/1‑140 (MQ=255)

                                   tttAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGt                                                                                                         <  1:3020197/135‑1 (MQ=255)

                                         gggCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACg                                                                                              >  2:632723/1‑140 (MQ=255)

                                          ggCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGt                                                                                             <  1:2437237/140‑1 (MQ=255)

                                          ggCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGt                                                                                             >  2:2588182/1‑140 (MQ=255)

                                          ggCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGt                                                                                             >  2:2823529/1‑140 (MQ=255)

                                          ggCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGt                                                                                             <  1:3618820/140‑1 (MQ=255)

                                           gCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGtt                                                                                            >  2:5103195/1‑140 (MQ=255)

                                            cGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTg                                                                                           >  1:974311/1‑140 (MQ=255)

                                            cGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTg                                                                                           >  2:1368601/1‑140 (MQ=255)

                                            cGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTg                                                                                           >  1:2003969/1‑140 (MQ=255)

                                            cGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTg                                                                                           <  1:1539761/140‑1 (MQ=255)

                                                     cgTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAatat                                                                                  >  2:1074812/1‑140 (MQ=255)

                                                     cgTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAatat                                                                                  >  1:1558590/1‑140 (MQ=255)

                                                         gCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCa                                                                              <  1:1101252/140‑1 (MQ=255)

                                                          cTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCAc                                                                             <  1:3858752/140‑1 (MQ=255)

                                                           tCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCAcc                                                                            <  2:3164109/140‑1 (MQ=255)

                                                           tCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCAcc                                                                            <  1:3075662/140‑1 (MQ=255)

                                                           tCATGTCCATGCCTCCCATTCCTCCGTGTGCGCTGTGCTCACCTTCTGGCTGGTTCTTGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGATAAATATTCCAcc                                                                            <  2:2135810/140‑1 (MQ=255)

                                                              tGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTTTGGCTGCGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTCATTACATCGGCAAACAAGTCTGGTCTCACGTTGGAGAAATATTCCACCTgg                                                                         <  1:3113699/140‑1 (MQ=255)

                                                              tGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTgg                                                                         <  1:3361806/140‑1 (MQ=255)

                                                              tGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTgg                                                                         <  2:3146269/140‑1 (MQ=255)

                                                              ggTCCATGCCTTACATTCCTTGGGGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACAGCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTgg                                                                         <  2:4967774/139‑1 (MQ=255)

                                                                    tGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTAt                                                                   >  1:4085892/1‑140 (MQ=255)

                                                                    tGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTAt                                                                   >  2:442131/1‑140 (MQ=255)

                                                                    tGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTAt                                                                   >  1:4194187/1‑140 (MQ=255)

                                                                    tGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTAt                                                                   >  1:1005623/1‑140 (MQ=255)

                                                                        ttCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGc                                                               >  1:4785627/1‑140 (MQ=255)

                                                                         tcaaTACCTTCGTGTGCGCTGTGCTCACCTTCTGACAGGGTCATGTCCATGCTCTTACCGGGAGCCATAAAATTGTTAATTACATCGGCAGATAAGTCAGGTTTTACGTTGGCGCAATATTCACCGTGGTTGTATTCGCt                                                              >  2:882438/4‑140 (MQ=255)

                                                                         tCCATACCTTCGTGTGCGGTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACACGTCTGGTTTCACGTTGGAGAAATATTCCGCCTGGTTGTATTCGCt                                                              >  2:2028850/1‑140 (MQ=255)

                                                                         tCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCACACAAGTCTGGTTTCACGTTGGAGAACTATTCCGCCGGGTTGTATTCGCt                                                              >  2:3557176/1‑140 (MQ=255)

                                                                         tCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCt                                                              >  2:4057102/1‑140 (MQ=255)

                                                                         tCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCt                                                              >  1:899268/1‑140 (MQ=255)

                                                                          ccATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAGCAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTa                                                             >  2:3965811/1‑140 (MQ=255)

                                                                          ccATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACTTTTGAGAAATATACCACTTGGTGGTATTCGCTa                                                             >  2:1621921/1‑140 (MQ=255)

                                                                          ccATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGCACTATTCCACCCGGTTGTATTCGCTa                                                             >  2:4931265/1‑140 (MQ=255)

                                                                                cTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGcgcg                                                       >  2:254472/1‑140 (MQ=255)

                                                                                    gtgtGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCa                                                   >  1:1381770/1‑140 (MQ=255)

                                                                                       tgCGCTGTGCTCACCTTGTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAAAAAGTCTGGTTTCACGTGGGAGAAATATTCCACCTGGTGGTATTCGCGAGGCGAGGCCAGtt                                                >  2:5189990/1‑140 (MQ=255)

                                                                                       tgCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGtt                                                >  1:520073/1‑140 (MQ=255)

                                                                                         cgcTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCACACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGtttt                                              >  2:2501651/1‑140 (MQ=255)

                                                                                         cgcTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGtttt                                              >  1:2311047/1‑140 (MQ=255)

                                                                                          gcTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACCTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGttttt                                             >  2:4164420/1‑140 (MQ=255)

                                                                                              tgCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGaa                                         >  2:402942/1‑140 (MQ=255)

                                                                                              tgCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGaa                                         >  2:1906869/1‑140 (MQ=255)

                                                                                              tgCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGACAGTTTTTCGaa                                         >  2:737952/1‑140 (MQ=255)

                                                                                               gCTCACCTTCTGGCTGGGTCATGTGCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAAc                                        <  1:2841891/140‑1 (MQ=255)

                                                                                                cTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACg                                       <  1:396531/140‑1 (MQ=255)

                                                                                                   aCCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAg                                    <  2:1274868/140‑1 (MQ=255)

                                                                                                   aCCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAg                                    <  1:2142768/140‑1 (MQ=255)

                                                                                                   aCCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAg                                    >  2:3277843/1‑140 (MQ=255)

                                                                                                   aCCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAg                                    <  1:4409770/140‑1 (MQ=255)

                                                                                                    ccTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGc                                   <  2:2050693/140‑1 (MQ=255)

                                                                                                    ccTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGc                                   <  1:2229664/140‑1 (MQ=255)

                                                                                                     cTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGcc                                  <  2:4858321/140‑1 (MQ=255)

                                                                                                       tCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCAt                                <  1:352198/140‑1 (MQ=255)

                                                                                                        ctggctggGTCATGTCCATGCTCTTCCCGTGAGCCATAAACTTGTTAATTACATCGGCACACAAGTCTGGTTTCACGTTGGAGACATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATg                               >  2:935613/1‑140 (MQ=255)

                                                                                                        ctggctggGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATg                               >  1:2626004/1‑140 (MQ=255)

                                                                                                        ctggctggGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATg                               >  1:4449287/1‑140 (MQ=255)

                                                                                                        ctggctggGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATg                               >  2:3369052/1‑140 (MQ=255)

                                                                                                        ctggctggGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATg                               >  2:3653402/1‑140 (MQ=255)

                                                                                                        ctggctggGTCATGTCCATGCTCTTACCGTGAGCCATAAACTGGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCGGTTTTTCGAACGCAGCCATg                               >  2:113563/1‑140 (MQ=255)

                                                                                                        ctggctggGACAAGTCCATGCGCTTACCGTGGGCCGTCAGCTTGTTAATTACCTCGGCAAACAAGCATGGTTTCACGCTGGAGAAATATTCCACCTGGTAGTATTAGCTAGACGCGGCCAGTTTCCAGAAAGCAGCCATg                               >  2:1753304/1‑140 (MQ=255)

                                                                                                          ggctggGTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAGTTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAGCGCAGCCATGTc                             >  1:4437926/1‑140 (MQ=255)

                                                                                                               ggacATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTCAGTACATGGGCAAACAAGGCTGGTTTCACGTTGGAGAAATATTCGACCTGGTTGTATTCGCTAGCTGCGCCCACTTTTTCGAACGCAGCAATGTCAGACa                        >  2:4682933/4‑140 (MQ=255)

                                                                                                               ggTCATGTCCATGCTCTTACCGTGAGCCATAAAGTTGTTAATTACATCGGCAAACAAGTCTAGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACCCAGCCATGTCAGACa                        >  2:2239244/1‑140 (MQ=255)

                                                                                                               ggTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACa                        >  1:1798439/1‑140 (MQ=255)

                                                                                                               ggTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACa                        <  2:4908993/140‑1 (MQ=255)

                                                                                                               ggTCATGTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACa                        <  1:1607829/140‑1 (MQ=255)

                                                                                                                   aTGTCCATGCTCTTACCGTGAGCCATACACTTGTTAATTACATCGGCATACAAGTCTGGTTTCACGTTGGAGAAATATTCCACATGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGACAGACATGgt                    >  2:3808870/1‑140 (MQ=255)

                                                                                                                     gTCCATGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGgtgt                  <  2:3329737/140‑1 (MQ=255)

                                                                                                                          tGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGc             >  2:3458837/1‑140 (MQ=255)

                                                                                                                          tGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGc             >  1:2075034/1‑140 (MQ=255)

                                                                                                                          tGCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGc             >  1:1306073/1‑140 (MQ=255)

                                                                                                                           gCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGTCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTATTCGGCg            >  2:124445/1‑140 (MQ=255)

                                                                                                                           gCTCTTACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCg            <  1:632722/140‑1 (MQ=255)

                                                                                                                                tACCGTGAGCCATAAACTTGTTAATTACATCGGCAAAc                                                                                                             >  1:3748678/1‑38 (MQ=255)

                                                                                                                                tACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCAc                                                                             >  1:1672933/1‑70 (MQ=255)

                                                                                                                                tACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGc       >  1:133442/1‑140 (MQ=255)

                                                                                                                                tACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGc       >  2:863916/1‑140 (MQ=255)

                                                                                                                                tACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGc       >  1:4629472/1‑140 (MQ=255)

                                                                                                                                tACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGc       >  1:2200649/1‑140 (MQ=255)

                                                                                                                                tACCGTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGc       >  1:3400711/1‑140 (MQ=255)

                                                                                                                                 atcGTGAGCCATAAACTTGTTAATTAAATGGGCTAATAGGTCTGGTTTCACGTTGGAGAAATATTTCACCTGGTCGTATTCGCAAGGTGGGGCCAGTTTTTCTAGCGTATACATTTCAGACGTGGTGTTCGGCGACTGCt      >  2:2772074/3‑140 (MQ=255)

                                                                                                                                    gTGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCg   <  1:1233197/140‑1 (MQ=255)

                                                                                                                                     tGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCTACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGc  >  1:509551/1‑140 (MQ=255)

                                                                                                                                     tGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCGACCTGGTGGTGTTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGc  >  2:4332087/1‑140 (MQ=255)

                                                                                                                                     tGAGCCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGc  >  2:3119340/1‑140 (MQ=255)

TAATTTTCCGAACATCTTTATTCTTCCTCAACCCCTTTAATGGGCGGATTCCGCGTGGCTCATGTCCATGCCTTCCATACCTTCGTGTGCGCTGTGCTCACCTTCTGGCTGGGTCATGTCCATGCTCTTACCGTGAGTCATAAACTTGTTAATTACATCGGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGC  >  NZ_CP025268/449677‑449949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`