BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	49,766	T→C	intergenic (+134/‑58)	kefC → / → folA	glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	49,766	0	T	C	100.0%	838.8 / NA	253	intergenic (+134/‑58)	kefC/folA	glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (126/127); total (126/127)

Only 100 of 255 total aligned reads displayed.

AATAAAGAGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCC  >  NZ_CP025268/49631‑49897

aataaAGAGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCgacg                                                                                                                                 <  2:827229/140‑1 (MQ=255)

aataaAGAGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCgacg                                                                                                                                 <  1:3531542/140‑1 (MQ=255)

aataaAGAGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCgacg                                                                                                                                 <  1:2242120/140‑1 (MQ=255)

aataaAGAGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCgacg                                                                                                                                 <  1:3951794/140‑1 (MQ=255)

aataaAGAGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATGTTCCTCAACATCATCCTCGCACCAGTCgacg                                                                                                                                 <  2:2263557/140‑1 (MQ=255)

aataaAGAGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTAGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCgacg                                                                                                                                 <  2:1744346/140‑1 (MQ=255)

 ataaAGAGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCgacg                                                                                                                                 >  2:3989418/1‑139 (MQ=255)

 ataaAGAGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCgacg                                                                                                                                 <  1:3989418/139‑1 (MQ=255)

  taaAGAGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTTATTGTAATGCGGCGAGTCCAGGGTGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCgacgac                                                                                                                               <  2:4157238/140‑1 (MQ=255)

  taaAGAGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCgacgac                                                                                                                               <  1:808543/140‑1 (MQ=255)

       agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGttt                                                                                                                          >  2:2915187/1‑140 (MQ=255)

       agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGttt                                                                                                                          <  2:2677433/140‑1 (MQ=255)

       agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGttt                                                                                                                          >  2:3893751/1‑140 (MQ=255)

       agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGttt                                                                                                                          <  2:4535831/140‑1 (MQ=255)

       agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGttt                                                                                                                          >  2:602334/1‑140 (MQ=255)

       agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGttt                                                                                                                          >  1:1158168/1‑140 (MQ=255)

       agTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGttt                                                                                                                          <  1:5013706/140‑1 (MQ=255)

        gTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCgacg                                                                                                                                 >  2:4413921/1‑132 (MQ=255)

        gTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCgacg                                                                                                                                 <  1:4413921/132‑1 (MQ=255)

        gTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTa                                                                                                                         <  2:1638072/140‑1 (MQ=255)

         tGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTAc                                                                                                                        >  1:3663054/1‑140 (MQ=255)

         tGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTAc                                                                                                                        >  2:3354607/1‑140 (MQ=255)

         tGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTAc                                                                                                                        >  2:3355070/1‑140 (MQ=255)

         tGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTAc                                                                                                                        <  2:4188153/140‑1 (MQ=255)

         tGACATACATCACACTTAAAAGCTAACTAGTTGTTTTCGATTCATTGTAATGCGGCGAGTCCAGGGAGAGAACGAGCACTCGTGAACAGAATATAAAATTATCATCAACATCATCCTCGCACCAGTCGACGACGGTTTAc                                                                                                                        <  2:445052/140‑1 (MQ=255)

           aCGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCTGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGc                                                                                                                      >  2:5093843/1‑140 (MQ=255)

           aCGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGTCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTTAACATGATCCTCGCACCAGTCGGCGACGGTTTActc                                                                                                                      >  2:4338537/1‑138 (MQ=255)

           aCGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGc                                                                                                                      >  2:1878297/1‑140 (MQ=255)

           aCGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGc                                                                                                                      <  1:340147/140‑1 (MQ=255)

           aCGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGc                                                                                                                      >  2:1356442/1‑140 (MQ=255)

           aCGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGc                                                                                                                      <  1:4692697/140‑1 (MQ=255)

            cGTACATCACACTTTACCGCTAACTATTTGTTTTGGTTTCATTGTAATGCGGCGAGTCCAGGGAGCGAGATTGGACTCGCCAGCAGATTATACACTTTTCCTCAACAACATCCTCGCACCCGTCTACGACGGTTTACGCt                                                                                                                     <  2:1020022/140‑1 (MQ=255)

            cGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCt                                                                                                                     <  1:3232528/140‑1 (MQ=255)

              tAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCttt                                                                                                                   <  1:3480286/140‑1 (MQ=255)

                 aTCACACTTTACAGCTAACTGTTTGTTGTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTGCGTGTTACg                                                                                                                >  2:4283956/1‑140 (MQ=255)

                          tACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGc                                                                                                       >  1:1336031/1‑140 (MQ=255)

                            cAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGa                                                                                                     <  1:1892133/140‑1 (MQ=255)

                               cTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACaa                                                                                                  >  1:21967/1‑140 (MQ=255)

                                  aCTGTTTGTTTTTGTTTCAGTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGCTTACGCTTTACGTATAGTGGCGCCAAttt                                                                                               >  2:3640519/1‑140 (MQ=255)

                                  aCTGTTGGTGTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACACttt                                                                                               >  1:1010611/1‑140 (MQ=255)

                                    tgtttgttTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAttttt                                                                                             >  2:1147458/1‑140 (MQ=255)

                                       ttgtttttgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAtttttttt                                                                                          >  1:1016424/1‑140 (MQ=255)

                                         gtttttgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCgacg                                                                                                                                 <  1:2375550/99‑1 (MQ=255)

                                         gtttttgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCgacg                                                                                                                                 >  2:2375577/1‑99 (MQ=255)

                                             ttgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCggg                                                                                    <  1:237833/140‑1 (MQ=255)

                                              tgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGa                                                                                   <  1:602334/140‑1 (MQ=255)

                                              tgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGa                                                                                   <  1:22099/140‑1 (MQ=255)

                                                tttCATTGTGAACGGGCGGTTCGAGGGACAGCGCGTGGATTCTCCAGCAGAATATATATGTTCCGTCAACATCATCCACGCACCAGTCGACGACGGTTTACGGTTTACGTATAGTGGCGACAATTTTTTTTATAGAGaaa                                                                                 <  2:556898/140‑1 (MQ=255)

                                                tttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaaa                                                                                 <  1:1065594/140‑1 (MQ=255)

                                                tttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaaa                                                                                 <  2:2490092/140‑1 (MQ=255)

                                                    aTTATAATGCGGCGAGACCAGAGAGAGAGCGCGCACTCACCAACAGAATATAAAATCTTCCTCAACATCATCCTCGCACCACACCACGCCGGTTCACACTTTACCTCTACTAGCGACTACATTTTTTCTCGCGaaaaaac                                                                             >  2:3006852/1‑136 (MQ=255)

                                                        tAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATg                                                                         >  1:702008/1‑140 (MQ=255)

                                                        tAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATg                                                                         <  2:1918680/140‑1 (MQ=255)

                                                          aTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGAt                                                                       <  1:3162440/140‑1 (MQ=255)

                                                          aTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGAt                                                                       <  2:2605131/140‑1 (MQ=255)

                                                          aTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGAt                                                                       <  1:4484498/140‑1 (MQ=255)

                                                              ggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAgt                                                                   <  2:3322114/140‑2 (MQ=255)

                                                              ggcgAGTCCAGGGAGAGAGCGTGCACCCGCCAGCAGAATATAACATTTTCCTCAACATCCTCCTCGCACCTGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAACCTCAATGATCAgt                                                                   <  2:2795783/140‑2 (MQ=255)

                                                               gcgAGTCCAGGGAGAGTGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATACTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAgtc                                                                  <  1:2881173/140‑3 (MQ=255)

                                                                cgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAgtct                                                                 >  2:376552/1‑137 (MQ=255)

                                                                cgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCt                                                                 >  1:4218851/1‑140 (MQ=255)

                                                                cgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCt                                                                 >  2:774474/1‑140 (MQ=255)

                                                                    tCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATATAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGAtt                                                             <  1:5137750/140‑1 (MQ=255)

                                                                           aggtagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                      <  2:2017757/136‑1 (MQ=255)

                                                                           agagagCGTGGACTCGCCAGCAGAATATAATATTTTCCTCGACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACACTTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                      <  1:2725210/140‑1 (MQ=255)

                                                                           agagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                      <  1:2095237/140‑1 (MQ=255)

                                                                           agagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                      <  1:178423/140‑1 (MQ=255)

                                                                           agagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                      <  2:936110/140‑1 (MQ=255)

                                                                           agagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                      <  1:3846255/140‑1 (MQ=255)

                                                                           agagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                      <  1:5043128/140‑1 (MQ=255)

                                                                           agagagCGTGGAATCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACCATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGt                                                      <  1:4438570/140‑1 (MQ=255)

                                                                             agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTa                                                    >  1:844452/1‑140 (MQ=255)

                                                                              gagCGTGGACTCGCCAGCAGAATATGAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAg                                                   >  2:4659576/1‑140 (MQ=255)

                                                                              gagCGTGGACTCGCCAGCAGAATATAAGATTTTCCTCAATAGCATCCTCGCACCCGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTGTTATCGGGAAATCGCAATGATCAGTCTGATTGCGGCGTGAg                                                   >  1:1168354/1‑140 (MQ=255)

                                                                              gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGGGTTAg                                                   >  2:4580039/1‑140 (MQ=255)

                                                                              gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAg                                                   >  1:697215/1‑140 (MQ=255)

                                                                              gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAg                                                   >  1:1616018/1‑140 (MQ=255)

                                                                              gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAg                                                   >  1:1676798/1‑140 (MQ=255)

                                                                              gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAg                                                   >  1:495435/1‑140 (MQ=255)

                                                                              gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAg                                                   >  1:3129425/1‑140 (MQ=255)

                                                                              gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAg                                                   >  1:4109432/1‑140 (MQ=255)

                                                                              gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAg                                                   >  1:3765070/1‑140 (MQ=255)

                                                                              gagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAGAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAg                                                   >  1:1970038/1‑140 (MQ=255)

                                                                                    ggACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAg                                             >  1:2983256/1‑140 (MQ=255)

                                                                                      aCTCGCCAGCAGACTATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGAt                                           <  1:4573523/140‑1 (MQ=255)

                                                                                       cTCGCCAGCAGAATATGAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATc                                          >  2:2723527/1‑140 (MQ=255)

                                                                                              gcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGCCGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTAt                                   <  2:3196340/140‑1 (MQ=255)

                                                                                                  aaTATAACATTGTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTCTCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGc                               <  1:228264/140‑1 (MQ=255)

                                                                                                   atatAACATTTTCCTCCACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTAAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCa                              <  2:496048/140‑1 (MQ=255)

                                                                                                    tataAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCAt                             >  1:4861518/1‑140 (MQ=255)

                                                                                                        aaaTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGaa                         <  2:4886119/140‑1 (MQ=255)

                                                                                                            tttCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACg                     >  2:1524673/1‑140 (MQ=255)

                                                                                                              tCCTCAACATCATCCTCGCACCAGTCGACGGCGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATGGGGAAATCTCAATGATGAGTCTGATTGCGGCGTTAGCGGTAGATTGTGTTATCGGCATGGAAAACGca                   >  2:2549673/1‑139 (MQ=255)

                                                                                                              tCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGcc                   >  2:1431427/1‑140 (MQ=255)

                                                                                                              tCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGcc                   >  2:3913835/1‑140 (MQ=255)

                                                                                                               ccTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCTTGGAAAACGCCa                  <  2:5137254/140‑1 (MQ=255)

                                                                                                                   aaCATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcggc                                                        <  2:1644707/98‑1 (MQ=255)

                                                                                                                    aCATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCg             <  1:2157698/140‑1 (MQ=255)

                                                                                                                     catcatCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGAGCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGt            >  2:2853684/1‑140 (MQ=255)

                                                                                                                               gCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgcc  >  2:3390947/1‑140 (MQ=255)

AATAAAGAGTGACGTAAATCACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTTGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGCCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCC  >  NZ_CP025268/49631‑49897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`