Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 4,608,009 (G)9→8 noncoding (72/88 nt) CXP41_RS23865 ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252684,608,0010G.96.3% 712.6 / 20.5 166noncoding (80/88 nt)CXP41_RS23865tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base G (6/0);  new base . (79/79);  total (85/81)
Fisher's exact test for biased strand distribution p-value = 2.90e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 191 total aligned reads displayed.

ACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGA  >  NZ_CP025268/4607863‑4608136
                                                                                                                                          |                                                                                                                                       
acaAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGCAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAgg                                                                                                                                        <  1:1366671/140‑1 (MQ=255)
 caAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAGTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAggg                                                                                                                                       >  1:1475386/1‑140 (MQ=255)
 caAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAggg                                                                                                                                       >  2:2552275/1‑140 (MQ=255)
 caAACAGTTGCGGCATTACATTTTGCTACCATCGGGCCTGGCACTTATTGCTGGCATCATGCTGATGATTATGATTTTGAATCATTGATTAAAAATCAAGGAAGAAACAATAAAGGGAGTAAAGATAATTGGTGCGAggg                                                                                                                                       >  2:1418919/1‑140 (MQ=255)
   aaCAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAggggg                                                                                                                                     <  2:384876/140‑1 (MQ=255)
    aCAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGTTGCGAgggggg                                                                                                                                    >  2:3232783/1‑140 (MQ=255)
      aGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAgggggggg                                                                                                                                  >  1:680412/1‑140 (MQ=255)
      aGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAgggggggg                                                                                                                                  >  1:3524769/1‑140 (MQ=255)
      aGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAgggggggg                                                                                                                                  >  1:2552515/1‑140 (MQ=255)
        ttGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAgggggggggc                                                                                                                                >  1:4402005/1‑139 (MQ=255)
        ttGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAggggggggac                                                                                                                                >  1:1644565/1‑138 (MQ=255)
        ttGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAggggggggac                                                                                                                                >  2:500905/1‑138 (MQ=255)
        ttGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAggggggggac                                                                                                                                >  1:1834964/1‑138 (MQ=255)
        ttGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAggggggggac                                                                                                                                >  1:493195/1‑138 (MQ=255)
        ttGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTAAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAggggggggac                                                                                                                                >  2:4917839/1‑138 (MQ=255)
           cGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTg                                                                                                                            >  2:297052/1‑140 (MQ=255)
            ggCATTACATTTTGCTAACATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGa                                                                                                                           <  2:3477811/140‑1 (MQ=255)
                ttACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGCAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAAccc                                                                                                                       >  1:2901192/1‑140 (MQ=255)
                ttACATTTTGCTACCATCGGGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGAAAATTGGTGCGAGGGGGGGGGACTTGAAcc                                                                                                                        >  2:3749175/1‑140 (MQ=255)
                  aCATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAAgcccc                                                                                                                     >  2:1090646/1‑140 (MQ=255)
                   cATTTTGCTCCCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGAGAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCa                                                                                                                    <  1:809861/140‑1 (MQ=255)
                   cATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCa                                                                                                                    <  1:4714443/140‑1 (MQ=255)
                   cATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCa                                                                                                                    <  1:1487096/140‑1 (MQ=255)
                   cATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATCAATTTGAAGCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGCAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCa                                                                                                                    <  2:162692/140‑1 (MQ=255)
                    aTTTTGCTACCATCGTGCCTGGCACTGATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAACCAAGGAAGAAACAAGAAAGTAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCAc                                                                                                                   <  2:2252950/140‑1 (MQ=255)
                     ttttGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACg                                                                                                                  <  2:1436571/140‑1 (MQ=255)
                        tGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTcc                                                                                                               >  1:510600/1‑140 (MQ=255)
                        tGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTcc                                                                                                               >  1:1548906/1‑140 (MQ=255)
                          cTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGt                                                                                                             >  2:334641/1‑140 (MQ=255)
                             ccATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                          <  1:2783442/140‑1 (MQ=255)
                             ccATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                          <  1:4293851/140‑1 (MQ=255)
                             ccATCGTGCCTGGCACTTATTGCTGGAATCATGCTGATGATAATGAATTTGAATCAGGGATAAAAAATCAAGGAAGAAACAAGAAAGGAAGAAAAGATAATTGGCGC‑AAGGGGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                          <  2:4992600/140‑1 (MQ=39)
                              cATGGTGCCTGGCACTTATTGCTGGAATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAACAAAGGAAGAAACAAGAAAGGAAGTAAGGATAATTGGGGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAgg                                                                                                         <  2:3041550/140‑1 (MQ=38)
                               agcGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAATATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGa                                                                                                        <  2:4465721/138‑1 (MQ=255)
                                tCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAAGGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATGGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGac                                                                                                       >  2:4422300/1‑140 (MQ=255)
                                   tGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                                                                                                    <  2:4323075/140‑1 (MQ=255)
                                     ccTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                                  <  2:1909325/140‑1 (MQ=255)
                                     ccTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                  <  2:4391326/140‑1 (MQ=255)
                                     ccTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATAAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                                  <  2:4802904/140‑1 (MQ=255)
                                        ggCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAacac                                                                                               >  1:4484461/1‑140 (MQ=255)
                                        ggCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAacac                                                                                               >  2:2689183/1‑140 (MQ=255)
                                             ttATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGaa                                                                                          >  2:2205833/1‑140 (MQ=255)
                                               aTTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAGTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGc                                                                                        >  2:2158065/1‑140 (MQ=255)
                                               aTTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGc                                                                                        >  2:3451420/1‑140 (MQ=255)
                                               aTTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGc                                                                                        >  1:1182477/1‑140 (MQ=255)
                                                ttGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt                                                                                       <  2:708268/140‑1 (MQ=255)
                                                    tGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg                                                                                   >  1:3589916/1‑140 (MQ=255)
                                                     ggCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                                                                                  >  1:2418556/1‑140 (MQ=255)
                                                     ggCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                                                                                  <  2:595653/140‑1 (MQ=255)
                                                      gCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAggggg                                                                                                                                     >  1:3475112/1‑89 (MQ=255)
                                                        atcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                                                                               >  2:2123960/1‑140 (MQ=255)
                                                        atcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                                                                               >  1:4250659/1‑140 (MQ=255)
                                                             gCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                          <  2:2151679/140‑1 (MQ=255)
                                                                gatgatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                                                                       >  2:5238644/1‑140 (MQ=255)
                                                                   gatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                    >  2:4343432/1‑140 (MQ=255)
                                                                    atAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                   <  1:433227/140‑1 (MQ=255)
                                                                    atAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                   <  1:1748964/140‑1 (MQ=255)
                                                                    atAATGAATTTAAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                   <  1:3668058/140‑1 (MQ=255)
                                                                      aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAGCAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCAGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                                 >  2:1295683/1‑140 (MQ=255)
                                                                      aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                                 >  1:767326/1‑140 (MQ=255)
                                                                       ctGAATTTGGAGCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                                                                <  2:1117560/139‑1 (MQ=255)
                                                                        tGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                               >  1:607093/1‑140 (MQ=255)
                                                                        tGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGCACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                               <  1:1162351/140‑1 (MQ=255)
                                                                           aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                            >  1:4148892/1‑140 (MQ=255)
                                                                           aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                            >  1:3741110/1‑140 (MQ=255)
                                                                           aTTTGAATAAGGGGTGAAGAATGAAGGGAGAAACAAGAAAGGAAGTAAAGATAAATGGTGCGA‑GAAGGGGGACTGGAACCAGAACGGCAGTAAGGAAACTAACAAAGAAAGCTAGAGCCTCTACCAAATCCGCAACCTTc                                                            >  2:228079/1‑140 (MQ=255)
                                                                               gAATCAGGGATGAAAACTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑AGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcac                                                        <  1:2957595/140‑1 (MQ=255)
                                                                               gAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcac                                                        <  1:5093400/140‑1 (MQ=255)
                                                                                 aTCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTCCGCACAg                                                      <  1:3083043/140‑1 (MQ=255)
                                                                                    aGGGATGAAAAATCTAGGAATAAGCAAGAGAGGAAGTGAAGATAATTGGTGCGA‑GGGGGGGGAGGTGAACACCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGGCa                                                   >  2:4130406/1‑140 (MQ=17)
                                                                                      ggATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCaca                                                 >  1:755070/1‑138 (MQ=255)
                                                                                      ggATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATc                                                 >  1:938302/1‑140 (MQ=255)
                                                                                      ggATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATc                                                 >  1:304948/1‑140 (MQ=255)
                                                                                      ggATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATc                                                 >  1:275226/1‑140 (MQ=255)
                                                                                            aaaaTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACACGCATCTCACtt                                           >  2:1807202/1‑140 (MQ=18)
                                                                                                tCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACtttttt                                       >  1:2139275/1‑140 (MQ=255)
                                                                                                    ggAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGat                                   <  1:2156377/140‑1 (MQ=255)
                                                                                                     gaaggaACAAGAAAGGAAGTACATATAAATGGAGAGA‑GGGGGGGGACTTGACCCCCCACGTCCGTAAGGACACAAACACCTGAAGCTAGCGCTGGTACCAATTCCGCCACCTGCGCACAGTCAGCTTACTTTTTTTGgta                                  <  2:2858932/140‑1 (MQ=17)
                                                                                                       agaaACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATc                                <  2:353109/140‑1 (MQ=255)
                                                                                                         aaaCAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGc                              <  1:5238644/140‑1 (MQ=255)
                                                                                                         aaaCAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGc                              <  1:232142/140‑1 (MQ=255)
                                                                                                         aaaCAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGc                              <  1:3986176/140‑1 (MQ=255)
                                                                                                             aaGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCg                          <  2:3371669/140‑1 (MQ=255)
                                                                                                                    gtggTACAGATACTTGCTGCG‑CGGGGGGGGACTTGAACCCCCACGGCCGTAAGGCCACTAGCACATGAGGCTAGCGCGTCTACCAACTCAGCCACCGAAGAACAGTGATCTTACTTTTTTTGATATCGCCTCGTTTGGTg                   <  2:1963316/137‑1 (MQ=12)
                                                                                                                    gAAGTAAAGATAATTGGTGGGA‑GTGGGGGGACTGGAACTCCCACGTCAGTAAGGACACTAACACCTGATGCTTTCGCGTCTACCAATTCTGCCACCGTCTCACAGTGATAATACTTTTTTTGATATCGCCGCGtgtgttc                   >  2:4244812/1‑137 (MQ=12)
                                                                                                                    gAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTg                   >  2:3516997/1‑140 (MQ=255)
                                                                                                                      aGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCg                 <  1:3903334/140‑1 (MQ=255)
                                                                                                                      aGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCg                 <  2:4655385/140‑1 (MQ=255)
                                                                                                                         aaaGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgg              <  2:2456122/140‑1 (MQ=255)
                                                                                                                            gagaaTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggggg           <  2:5179842/137‑1 (MQ=255)
                                                                                                                            gATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggggg           <  1:2508482/140‑1 (MQ=255)
                                                                                                                            gATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggggg           <  1:2205833/140‑1 (MQ=255)
                                                                                                                            gATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggggg           <  1:334641/140‑1 (MQ=255)
                                                                                                                            gATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggag           <  1:4280481/140‑3 (MQ=255)
                                                                                                                               aaTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggggg        <  1:2113266/140‑1 (MQ=255)
                                                                                                                               aaTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCACATTACTTTTTTTGATATCGTCTCGTTTGGTGCGAggcggggg        >  2:4768184/1‑140 (MQ=18)
                                                                                                                                 ttGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGAc      <  1:2661250/140‑1 (MQ=255)
                                                                                                                                    gTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGCTTg   >  1:3230125/1‑140 (MQ=255)
                                                                                                                                    gTGCGA‑GGGGGGGGACTTGAACATCCACGTCCGTAAGGACACTAACACCTGAAGCTAGAGCGCCTACTAATTCCGCCACCTTCGAACAGTCATCTTACTATTTTTGATATCGCCTCGTTGAGTGCGAggggggggttatg   >  2:1044206/1‑135 (MQ=18)
                                                                                                                                     tGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGa  <  2:3589916/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                       
ACAAACAGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGA  >  NZ_CP025268/4607863‑4608136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑