BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	581,197	+G	intergenic (+50/+4)	CXP41_RS24355 → / ← CXP41_RS03085	tail fiber assembly protein/class I SAM‑dependent methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	581,196	1	.	G	99.2%	1273.1 / 0.6	380	intergenic (+49/+5)	CXP41_RS24355/CXP41_RS03085	tail fiber assembly protein/class I SAM‑dependent methyltransferase
Reads supporting (aligned to +/- strand): ref base . (3/0); new base G (178/197); total (181/199)
Fisher's exact test for biased strand distribution p-value = 1.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 392 total aligned reads displayed.

AGCCTGGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTC  >  NZ_CP025268/581057‑581330

aGCCTGGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑Gtt                                                                                                                                         >  1:147219/1‑140 (MQ=255)

aGCCTGGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑Gtt                                                                                                                                         <  1:4932122/140‑1 (MQ=255)

    tGGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTT‑ggat                                                                                                                                    >  1:4535814/1‑137 (MQ=255)

      gAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTT‑ggatgc                                                                                                                                  <  1:2893806/140‑6 (MQ=255)

                   gtgtTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTg                                                                                                                      <  1:3706344/140‑1 (MQ=255)

                        gCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCg                                                                                                                 <  1:5012321/140‑1 (MQ=255)

                           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGaa                                                                                                              <  2:498862/140‑1 (MQ=255)

                           gAACCGTGTTGAGACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGaa                                                                                                              <  1:1172749/140‑1 (MQ=255)

                           gAAACGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGaa                                                                                                              <  2:3073546/140‑1 (MQ=255)

                                gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                         <  1:74980/140‑1 (MQ=255)

                                gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                         <  2:2616294/140‑1 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                        >  1:558485/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                        >  2:2357961/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                        >  1:3106383/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                        >  1:2679182/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTGTTCCTGAAGCGGAAAACAtt                                                                                                        >  2:4752175/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATAATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGACGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                        >  2:1511037/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAAGCATTGGGATTATGCCGCAGCACGTGTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAGCAtt                                                                                                        >  2:93251/1‑140 (MQ=255)

                                     gATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATtacta                                                                                                    <  2:4110811/140‑1 (MQ=255)

                                      aTACATCAACTGCACCTGATATTGAGTTGCCTATGAAACATATCAGGGGGTGATGATGAGGATTAACGCGCAGCACGCGTTAAGCGCGATCATACTGAGATTT‑GGTGCTATTTTTTTCCTGGAGCGGACAACACtactac                                                                                                   >  2:4536612/1‑140 (MQ=255)

                                      aTACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATtactac                                                                                                   >  1:1980740/1‑140 (MQ=255)

                                       tACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                  <  2:2453172/140‑1 (MQ=255)

                                       tACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                  <  1:4125145/140‑1 (MQ=255)

                                       tACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                  <  1:2187301/140‑1 (MQ=255)

                                       tACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                  <  2:3394246/140‑1 (MQ=255)

                                       tACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                  <  1:2278364/140‑1 (MQ=255)

                                       gaCATCAACTTCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATGGGGATTAGGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                  <  1:1784564/139‑1 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCTCGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTTTTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                              >  2:4314929/1‑140 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                              >  1:161048/1‑140 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                              >  1:4958515/1‑140 (MQ=255)

                                                   aCCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAcc                                                                                      <  2:2539087/140‑1 (MQ=255)

                                                   aCCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAcc                                                                                      <  1:3581416/140‑1 (MQ=255)

                                                   aCCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAcc                                                                                      <  1:3916098/140‑1 (MQ=255)

                                                    ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                     >  2:1071563/1‑140 (MQ=255)

                                                    ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                     <  1:4472795/140‑1 (MQ=255)

                                                    ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                     >  1:1042398/1‑140 (MQ=255)

                                                    ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                     <  2:558485/140‑1 (MQ=255)

                                                       gATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTgg                                                                                  >  2:843403/1‑140 (MQ=255)

                                                       gATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTgg                                                                                  >  1:107481/1‑140 (MQ=255)

                                                            tGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTa                                                                             >  1:4044812/1‑140 (MQ=255)

                                                            tGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTa                                                                             >  1:1661697/1‑140 (MQ=255)

                                                             gAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                            <  2:5308888/140‑1 (MQ=255)

                                                             gAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                            <  2:4874102/140‑1 (MQ=255)

                                                             gAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                            <  1:1885603/140‑1 (MQ=255)

                                                              aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                           >  2:3251019/1‑140 (MQ=255)

                                                              aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                           >  1:613349/1‑140 (MQ=255)

                                                              aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                           >  1:5098939/1‑140 (MQ=255)

                                                              aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                           >  2:749859/1‑140 (MQ=255)

                                                               gTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGaaaa                                                                                                            <  1:2222334/106‑1 (MQ=255)

                                                                  gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                       >  1:271291/1‑140 (MQ=255)

                                                                   ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGCTGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtct                                                                      >  1:1223714/1‑140 (MQ=255)

                                                                   ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtct                                                                      >  1:907465/1‑140 (MQ=255)

                                                                   ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtct                                                                      >  2:2286087/1‑140 (MQ=255)

                                                                   ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtct                                                                      >  1:2490028/1‑140 (MQ=255)

                                                                     tACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCg                                                                    >  1:3266274/1‑140 (MQ=255)

                                                                      aCGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                   <  1:4301674/140‑1 (MQ=255)

                                                                      aCGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGa                                                                   >  1:1553581/1‑140 (MQ=255)

                                                                       cGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaa                                                                  <  1:5343948/140‑1 (MQ=255)

                                                                       cGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCCTAGGCAAGAACATGCTGCG‑GATGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGCACCGTGTACCTTGGCTTTAACATTCTCGaa                                                                  <  1:3192867/140‑1 (MQ=255)

                                                                        gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                 <  2:311405/140‑1 (MQ=255)

                                                                            ccTGTCAGGGAGTACTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                             <  2:2023294/140‑1 (MQ=255)

                                                                            ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                             >  1:4589195/1‑140 (MQ=255)

                                                                            ccTGCCAGGGCGTAATAATTCGGATTATGCCCCAGCCCGTCTTAAGCAACACCATGCTACG‑CTTGGCTGCTATTTTTTTCATGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                             <  2:1387133/140‑1 (MQ=255)

                                                                             ccgTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCCGAAGCGGAAAACATTACTACAGTACCTAGAACCTTGGTTTTAGCATTCTCGAAATGctc                                                            >  1:2972198/3‑140 (MQ=255)

                                                                             cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACAGTCTCGAAATGctc                                                            >  2:4590483/1‑140 (MQ=255)

                                                                              tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                           <  1:2812476/140‑1 (MQ=255)

                                                                              tGTCAGGGAGGAATCATTGGGCTTATGCCGCAGCACGTCATAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAAGATTACGACAGTAACTTGAACCTTGGTTTTAACATTCTCGAAATGatct                                                           >  2:1739647/1‑140 (MQ=255)

                                                                               gTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTg                                                          >  1:2351899/1‑140 (MQ=255)

                                                                                taaGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATGTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCGCGAAATGCTCTga                                                         <  1:3084603/138‑1 (MQ=255)

                                                                                tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                         <  1:4675438/140‑1 (MQ=255)

                                                                                tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                         <  1:114659/140‑1 (MQ=255)

                                                                                tCAGGGAGTAAGCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAAGGCTCTga                                                         <  2:1854761/140‑1 (MQ=255)

                                                                                 cAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTgag                                                        <  2:2765523/140‑1 (MQ=255)

                                                                                   gggAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGGTTTAACATTCTCGAAATGCTCTGagag                                                      <  2:3926336/140‑1 (MQ=255)

                                                                                      aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                   <  2:1907103/140‑1 (MQ=255)

                                                                                      aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACCACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTgagcgcat                                                   >  2:4669688/1‑135 (MQ=255)

                                                                                       gTAATCATTGGGATTATGCCGCAGCACGTTTTAAGTAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCCGAAAACATTACCACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtata                                                  <  2:3009968/140‑1 (MQ=255)

                                                                                       gTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtata                                                  <  2:3188030/140‑1 (MQ=255)

                                                                                       gTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtata                                                  <  2:2190858/140‑1 (MQ=255)

                                                                                        tAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTatat                                                 >  2:3683519/1‑140 (MQ=255)

                                                                                          aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATAtgt                                               >  2:2674378/1‑140 (MQ=255)

                                                                                                gggATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAg                                         >  1:1200616/1‑140 (MQ=255)

                                                                                                 ggATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                             >  2:3506332/1‑119 (MQ=255)

                                                                                                  gATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                             >  2:322635/1‑118 (MQ=255)

                                                                                                  gATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGcc                                       >  2:4030988/1‑140 (MQ=255)

                                                                                                    ttATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTCCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCctt                                     <  1:4780932/140‑1 (MQ=255)

                                                                                                      aTGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttct                                   >  2:2925094/1‑140 (MQ=255)

                                                                                                      aTGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttct                                   >  2:638507/1‑140 (MQ=255)

                                                                                                       tGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttctt                                  >  1:5288796/1‑140 (MQ=255)

                                                                                                            cagcaCGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGaa                             >  1:5204797/1‑140 (MQ=255)

                                                                                                            cagcaCGTCTTAAGCAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGaa                             >  1:3410741/1‑140 (MQ=255)

                                                                                                                          cAAGAACATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTACCATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGa               <  2:1970310/140‑1 (MQ=255)

                                                                                                                              aaCATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGtt                                            <  2:1313289/107‑1 (MQ=255)

                                                                                                                              aaCATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGtt                                            >  1:1313289/1‑107 (MQ=255)

                                                                                                                              aaCATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg           >  2:1644583/1‑140 (MQ=255)

                                                                                                                              aaCATGCTGCG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg           >  1:4847546/1‑140 (MQ=255)

                                                                                                                                  tgctgcG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                            <  2:1163534/71‑1 (MQ=255)

                                                                                                                                  tgctgcG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGc       >  1:4402778/1‑140 (MQ=255)

                                                                                                                                      gcG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGGTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt   <  2:694616/140‑1 (MQ=255)

                                                                                                                                      gcG‑GTTGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt   <  1:2624139/140‑1 (MQ=255)

AGCCTGGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCG‑GTT‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTC  >  NZ_CP025268/581057‑581330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`