BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	939,446	(A)_7→8	intergenic (‑289/‑255)	trxB ← / → lrp	thioredoxin‑disulfide reductase/leucine‑responsive transcriptional regulator Lrp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	939,439	1	.	A	95.2%	841.8 / 27.9	231	intergenic (‑282/‑262)	trxB/lrp	thioredoxin‑disulfide reductase/leucine‑responsive transcriptional regulator Lrp
Reads supporting (aligned to +/- strand): ref base . (5/6); new base A (79/139); total (84/147)
Fisher's exact test for biased strand distribution p-value = 5.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 282 total aligned reads displayed.

CTGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAACGGTCTTCTC  >  NZ_CP025268/939300‑939572

ctGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGgaag                                                                                                                                             >  2:4446109/1‑140 (MQ=255)

ctGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGgaag                                                                                                                                             >  1:2255075/1‑140 (MQ=255)

 tGCTACTTAAATTTCCCGCTTTATAAGGCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATATTGTACTAAGAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTATGCGAAAACAGTCGAGgaag‑‑a                                                                                                                                          >  2:3910896/1‑140 (MQ=255)

 tGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGCGgaag‑‑a                                                                                                                                          >  1:3768194/1‑140 (MQ=255)

 tGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGgaag‑‑a                                                                                                                                          >  1:3979043/1‑140 (MQ=255)

 tGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGgaag‑‑a                                                                                                                                          >  1:1567030/1‑140 (MQ=255)

   cTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑‑aa‑a                                                                                                                                       >  1:4081838/1‑140 (MQ=255)

   cTACTTAAATTTCCCGCATTATCATCCGATTAAATGATGAAACAACACCCCTGTTAATGAATATCTGGCATATTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGCAG‑‑aa‑a                                                                                                                                       >  2:4561678/1‑140 (MQ=255)

     aCTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑‑aa‑a‑aa                                                                                                                                    <  1:1293061/140‑1 (MQ=255)

      cTTAAATTTCCCGCATTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCAGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑‑aa‑a‑aaa                                                                                                                                   >  2:2052479/1‑140 (MQ=255)

        tAAATTTCCCGCTTTCTAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAGAACATTCGAGGAAG‑‑aa‑a‑aaa‑aa                                                                                                                                >  2:3740166/1‑139 (MQ=255)

          aaTTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑‑aa‑a‑aaa‑aaca                                                                                                                              <  2:4060650/140‑4 (MQ=255)

          aaTTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑‑aa‑a‑aaa‑aaca                                                                                                                              <  1:3030039/140‑4 (MQ=255)

          aaTTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑‑aa‑a‑aaa‑aaca                                                                                                                              <  1:2635261/140‑4 (MQ=255)

          aaTTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑‑aa‑a‑aaa‑aaca                                                                                                                              <  1:5244944/140‑4 (MQ=255)

          aaTTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑‑aa‑a‑aaa‑aaca                                                                                                                              <  2:5203830/140‑4 (MQ=255)

          aaTTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑‑aa‑a‑aaa‑aaca                                                                                                                              <  1:73986/140‑4 (MQ=255)

          aaTTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCAGGTTGCACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑‑aa‑a‑aaa‑aaca                                                                                                                              <  2:4933540/140‑4 (MQ=255)

            tttCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑‑aa‑a‑aaa‑aacagt                                                                                                                            <  1:267368/140‑6 (MQ=255)

            tttCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑‑aa‑a‑aaa‑aacagt                                                                                                                            <  1:4996825/140‑6 (MQ=255)

            tttCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑‑aa‑a‑aaa‑aacagt                                                                                                                            <  1:4871323/140‑6 (MQ=255)

            tttCCCGCGTTATAAGACGATTAAATGATGAATAAACGCCCATGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑‑aa‑a‑aaa‑aacagt                                                                                                                            <  2:333564/140‑6 (MQ=255)

                ccGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTc                                                                                                                        >  1:1064727/1‑140 (MQ=255)

                    tttATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTtat                                                                                                                    <  2:2718432/140‑1 (MQ=255)

                    tttATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTtat                                                                                                                    <  1:468029/140‑1 (MQ=255)

                    tttATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTtat                                                                                                                    <  1:1924898/140‑1 (MQ=255)

                      tataAGCCGATTCAATGATTAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTatat                                                                                                                  <  2:626399/140‑1 (MQ=255)

                      tataAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTGGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTatat                                                                                                                  <  1:4791151/140‑1 (MQ=255)

                           gCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCa                                                                                                             <  1:2276905/140‑1 (MQ=255)

                              gATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATaa                                                                                                          >  2:4705139/1‑140 (MQ=255)

                               aTTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGCTGTCTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAAc                                                                                                         >  1:3800245/1‑140 (MQ=255)

                                   aaTGATGAATCAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑‑AA‑A‑AAA‑ACCAGTATTCTTATATGCGCATAACcatg                                                                                                     <  2:3920593/140‑1 (MQ=255)

                                   aaTGATGAATAAACGCCCCTGTTAATTAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACcatg                                                                                                     <  1:678315/140‑1 (MQ=255)

                                   aaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACcatg                                                                                                     <  1:3122022/140‑1 (MQ=255)

                                   aaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACcatg                                                                                                     <  2:1447847/140‑1 (MQ=255)

                                   aaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACcatg                                                                                                     <  1:2802890/140‑1 (MQ=255)

                                   aaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACcatg                                                                                                     <  2:2427770/140‑1 (MQ=255)

                                   aaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACcatg                                                                                                     <  2:4131510/140‑1 (MQ=255)

                                   aaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACcatg                                                                                                     <  1:3389090/140‑1 (MQ=255)

                                   aaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACcatg                                                                                                     <  1:3665866/140‑1 (MQ=255)

                                   aaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACCAAAAATCGATGGTATGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACcatg                                                                                                     <  1:4432717/140‑1 (MQ=255)

                                   aaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTATAATAAAAATAGATGTTTTGCTTTAACAATCCACTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACcatg                                                                                                     <  2:1680001/140‑1 (MQ=255)

                                   aaTGATCAATAACCGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACcatg                                                                                                     <  2:3424169/140‑1 (MQ=255)

                                     tgatgaATAAACGCCCCTGTTAATGATTATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACcatgca                                                                                                   <  2:1912536/140‑1 (MQ=255)

                                     tgatgaATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACcatgca                                                                                                   <  2:2263028/140‑1 (MQ=255)

                                          aaTAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTaa                                                                                              >  1:3250528/1‑140 (MQ=255)

                                            tAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAAt                                                                                            <  1:3830477/140‑1 (MQ=255)

                                            tAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAAt                                                                                            <  1:1066383/140‑1 (MQ=255)

                                            tAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAAt                                                                                            <  1:2491383/140‑1 (MQ=255)

                                            tAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAAt                                                                                            <  2:5022049/140‑1 (MQ=255)

                                                    ccTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTtat                                                                                                                    <  2:1995812/108‑1 (MQ=255)

                                                    ccTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTtat                                                                                                                    >  1:1995812/1‑108 (MQ=255)

                                                      tGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTa                                                                                  >  1:2304087/1‑140 (MQ=255)

                                                          aaTGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGt                                                                              <  2:4437630/140‑1 (MQ=255)

                                                          aaTGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTg                                                                             <  2:2971057/140‑1 (MQ=255)

                                                           aTGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTg                                                                             <  2:16088/140‑1 (MQ=255)

                                                           aTGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTg                                                                             >  1:3060447/1‑140 (MQ=255)

                                                             gAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCt                                                                           >  1:1233772/1‑140 (MQ=255)

                                                                tatCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGTTGTTTTGCGAAAACATTCGAGGAAG‑‑AA‑A‑AAACA‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTatt                                                                        >  1:987852/1‑138 (MQ=255)

                                                                tatCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAA‑A‑AAA‑A‑CATTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCTTGCAAg                                                                         >  2:2589544/1‑140 (MQ=255)

                                                                tatCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGt                                                                        >  2:5145992/1‑140 (MQ=255)

                                                                tatCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGt                                                                        <  2:4210905/140‑1 (MQ=255)

                                                                 atCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCCGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATGCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTg                                                                       >  1:2412781/1‑140 (MQ=255)

                                                                  tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATACCCATGCATGTAAATACCATGTTTACCGTGCTAGTGa                                                                      >  1:1816950/1‑140 (MQ=255)

                                                                  tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGa                                                                      >  1:3519272/1‑140 (MQ=255)

                                                                  tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGa                                                                      >  1:3234236/1‑140 (MQ=255)

                                                                  tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGa                                                                      >  2:3114115/1‑140 (MQ=255)

                                                                    tGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGaaa                                                                    <  2:3465510/140‑1 (MQ=255)

                                                                     ggCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAAt                                                                   <  2:2372735/140‑1 (MQ=255)

                                                                           ttgtACTAAACATCGATGTTTTGCTTTGACAAGCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGt                                                             <  1:2844087/140‑1 (MQ=255)

                                                                           ttgtACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGt                                                             <  1:1885172/140‑1 (MQ=255)

                                                                           ttgtACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGt                                                             <  1:4389971/140‑1 (MQ=255)

                                                                           ttgtACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGt                                                             <  1:1414160/140‑1 (MQ=255)

                                                                           ttgtACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGt                                                             <  1:2146519/140‑1 (MQ=255)

                                                                           tggtACTAAAAATCGATGTTTTGTTTTGACAGTAATCTGGGGGTGTGCGTAAACATTTGAGGGAGA‑AA‑A‑AAA‑A‑AAGTATTCTTATATGCGCATAACCATGTATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGt                                                             >  2:4815519/3‑140 (MQ=255)

                                                                             gtACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTAt                                                           <  1:1455612/140‑1 (MQ=255)

                                                                                cTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGc                                                        >  2:3258546/1‑140 (MQ=255)

                                                                                cTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGc                                                        <  2:2294317/140‑1 (MQ=255)

                                                                                cTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGc                                                        >  2:3852198/1‑140 (MQ=255)

                                                                                   aaaaTCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTg                                                     >  2:4602890/1‑140 (MQ=255)

                                                                                      aTCGATGTTTTGCTTTGACAATCCCGTGGTGTTTTGCGAAAACATTCTCGGAAG‑‑AAGA‑GAA‑A‑CAGTATTCTGATATGGGCATAACCATGCATGTAAATACCATGTTCACCGTGCTAGTGAAATCTACGTATGGCGTGGAc                                                  >  2:1033547/1‑140 (MQ=255)

                                                                                        cGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACcatg                                                                                                     <  2:1820710/87‑1 (MQ=255)

                                                                                            gTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGc                                            <  1:4615123/140‑1 (MQ=255)

                                                                                                    ttGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTg                                    <  2:2001795/140‑1 (MQ=255)

                                                                                                          aTCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCg                              <  1:2603571/140‑1 (MQ=255)

                                                                                                            ccccTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGAt                            <  1:5145992/140‑1 (MQ=255)

                                                                                                              ccTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTc                               <  2:3788497/135‑1 (MQ=255)

                                                                                                              ccTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTc                               >  1:3788324/1‑135 (MQ=255)

                                                                                                              ccTGGTGTTGTGCGAGATCATGGGAAGAAG‑‑AA‑AGACA‑A‑CAGTATGATTATGTGCGCAGAACCATGCATGTATGTCCCATGTTTCCCGGCCTAGTGAAATCTCCGCATGGCGTGGACAGACGCCATTCGTGATGTCGATAg                          <  2:2503656/140‑1 (MQ=255)

                                                                                                               cTGGTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGc                         <  1:1654383/140‑1 (MQ=255)

                                                                                                                 ggTGTTTTGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTg                       >  2:2771641/1‑140 (MQ=255)

                                                                                                                     ttttGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCcac                   >  1:816930/1‑140 (MQ=255)

                                                                                                                       ttGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACaa                 >  2:930200/1‑140 (MQ=255)

                                                                                                                       ttGCGAAAACATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACaa                 >  2:1523311/1‑140 (MQ=255)

                                                                                                                             aaaCATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAAc           <  2:3604420/140‑1 (MQ=255)

                                                                                                                             aaaCATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCAACAAGGCAAc           >  2:1301614/1‑140 (MQ=255)

                                                                                                                              aaCATTCGAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAACg          <  1:897759/140‑1 (MQ=255)

                                                                                                                                    cGAGGAAG‑‑AA‑A‑AAA‑ACCAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAACACTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATCGCTGCCACAAGGCAACGGtcttc    <  2:2897558/140‑1 (MQ=255)

                                                                                                                                     gAGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTCTATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAACGGtcttct   <  2:1402068/140‑1 (MQ=255)

                                                                                                                                      aGGAAGA‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAACGGTCTtctc  <  1:2223682/140‑1 (MQ=255)

CTGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑‑AA‑A‑AAA‑A‑CAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAACGGTCTTCTC  >  NZ_CP025268/939300‑939572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`