BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,146,507	+T	pseudogene (1028/1643 nt)	flgK →	flagellar hook‑associated protein FlgK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,146,507	1	.	T	98.9%	959.3 / 1.2	264	pseudogene (1028/1643 nt)	flgK	flagellar hook‑associated protein FlgK
Reads supporting (aligned to +/- strand): ref base . (1/2); new base T (121/139); total (123/141)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 286 total aligned reads displayed.

ACGCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑G‑GACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGACGCCGGAT  >  NZ_CP025268/1146368‑1146639

 cGCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAg‑gt                                                                                                                                      <  1:3446962/140‑2 (MQ=255)

 cGCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAg‑gt                                                                                                                                      <  1:1254545/140‑2 (MQ=255)

 cGCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAg‑gt                                                                                                                                      <  2:5091239/140‑2 (MQ=255)

  gCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAg‑gtg                                                                                                                                     <  1:1063946/140‑3 (MQ=255)

  gCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAg‑gtg                                                                                                                                     <  1:3973554/140‑3 (MQ=255)

   cTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAg‑gtga                                                                                                                                    >  1:3726192/1‑137 (MQ=255)

    ttGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAg‑gtgac                                                                                                                                   >  1:2911038/1‑136 (MQ=255)

      ggACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCGCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGt                                                                                                                                 >  2:4032655/1‑140 (MQ=255)

      ggACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGt                                                                                                                                 >  2:5189269/1‑140 (MQ=255)

          aaCTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCg                                                                                                                             <  1:2446786/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACCCAAAGCCGGATTTGATGCTAACGTCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGAt                                                                                                                           >  1:160411/1‑140 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAAAG‑T‑GACGTTGCGAt                                                                                                                           >  2:4591559/1‑140 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGAt                                                                                                                           <  2:2310638/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGAt                                                                                                                           <  2:1798638/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGAt                                                                                                                           >  2:1731497/1‑140 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGAt                                                                                                                           <  1:82183/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGAt                                                                                                                           <  1:4313363/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGAt                                                                                                                           <  1:4247185/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGAt                                                                                                                           <  2:811225/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGAt                                                                                                                           <  1:2183098/140‑1 (MQ=255)

            cTGGCGCTGGCATCTGCCGAGGCTTTCAACACCCAACACAAAGCCAGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGCTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGAt                                                                                                                           <  2:4633161/140‑1 (MQ=255)

             tGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATc                                                                                                                          <  1:2635148/140‑1 (MQ=255)

             tGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATc                                                                                                                          >  2:4478751/1‑140 (MQ=255)

                cgcTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGt                                                                                                                       <  2:2603061/140‑1 (MQ=255)

                cgcTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGt                                                                                                                       <  1:4165771/140‑1 (MQ=255)

                cgcTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGt                                                                                                                       <  2:2349107/140‑1 (MQ=255)

                cgcTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGAGGTCTTTGCTATCGGTAAGCCCGCGGTGCTGGAAGACACGAAAAAC‑CAAG‑GTGACGTTTCGATCGGt                                                                                                                       >  1:2479546/1‑140 (MQ=255)

                  cTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGc                                                                                                                     <  2:3957867/140‑1 (MQ=255)

                  cTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGc                                                                                                                     >  2:1965724/1‑140 (MQ=255)

                   tGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGcc                                                                                                                    <  1:1727245/140‑1 (MQ=255)

                    ggCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGGGAAGATTTCTTTGCTATCGGTAAGCGCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCa                                                                                                                   >  1:4932764/1‑140 (MQ=255)

                     gCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCAc                                                                                                                  >  1:313571/1‑140 (MQ=255)

                     ccctttGCGCAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTTCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCAc                                                                                                                  <  2:4388028/137‑1 (MQ=255)

                       aTTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACgg                                                                                                                <  2:1087011/140‑1 (MQ=255)

                       aTTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACgg                                                                                                                <  1:5268892/140‑1 (MQ=255)

                          tGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTaa                                                                                                             >  2:2652014/1‑140 (MQ=255)

                              gAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGa                                                                                                         <  1:3647968/140‑1 (MQ=255)

                               aGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGAt                                                                                                        >  1:1009826/1‑140 (MQ=255)

                               aGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGAt                                                                                                        >  1:488781/1‑140 (MQ=255)

                               aGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCAGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACGGAt                                                                                                        >  2:2112648/1‑140 (MQ=255)

                                 gCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGc                                                                                                      <  2:2593633/140‑1 (MQ=255)

                                 gCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGc                                                                                                      <  2:2847903/140‑1 (MQ=255)

                                 gCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGc                                                                                                      <  2:3620745/140‑1 (MQ=255)

                                     tCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTcc                                                                                                  >  2:4359329/1‑140 (MQ=255)

                                      cAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAACC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                                 <  2:405809/140‑1 (MQ=255)

                                      cAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTATCTGATGCCTCcg                                                                                                 <  2:5282648/140‑1 (MQ=255)

                                      cAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                                 <  1:1991166/140‑1 (MQ=255)

                                      cAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                                 <  2:2200173/140‑1 (MQ=255)

                                      cAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                                 <  1:3471174/140‑1 (MQ=255)

                                      cAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                                 <  1:4406910/140‑1 (MQ=255)

                                      cAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGATTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                                 <  1:3905310/140‑1 (MQ=255)

                                            ccAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTAGCGGTAAGCCCGCGGTTCTGCAAAACACGAAAACC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGACGCCTCCGCGGTAc                                                                                           >  2:4916897/1‑140 (MQ=255)

                                              aaCACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTGTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTg                                                                                         >  1:2775716/1‑140 (MQ=255)

                                              aaCACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTg                                                                                         >  1:3303647/1‑140 (MQ=255)

                                              aaCACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTg                                                                                         >  2:3425514/1‑140 (MQ=255)

                                               acacAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTgg                                                                                        >  1:2811278/1‑140 (MQ=255)

                                                  caAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGa                                                                                     <  1:1303457/140‑1 (MQ=255)

                                                  caAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGa                                                                                     <  1:110981/140‑1 (MQ=255)

                                                   aaaGCCGGATTTGGTGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACCTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGAc                                                                                    >  2:2778853/1‑140 (MQ=255)

                                                    aaGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACa                                                                                   >  1:3423127/1‑140 (MQ=255)

                                                      gCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcgcg                                                                                               <  1:2026823/126‑1 (MQ=255)

                                                      gCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGa                                                                                 >  1:2426185/1‑140 (MQ=255)

                                                      gCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGa                                                                                 >  1:3526708/1‑140 (MQ=255)

                                                      gCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGa                                                                                 <  2:344592/140‑1 (MQ=255)

                                                          gATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTAc                                                                             >  1:3838363/1‑140 (MQ=255)

                                                          gATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTAc                                                                             >  1:2553668/1‑140 (MQ=255)

                                                          gATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTAc                                                                             >  2:4230486/1‑140 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                                  <  1:1342031/140‑1 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                                  <  2:651627/140‑1 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                                  <  1:4045006/140‑1 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                                  <  1:577619/140‑1 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                                  <  1:674419/140‑1 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTGCCTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTAGCGATCGGTGCAACGGTAACTGATGCCTCCGCGGTACTGGCGAAAGATTACAAAATATCGtt                                                                  <  2:4213424/140‑1 (MQ=255)

                                                                     aaCGGCGAGGCCGGTGAAGATTTCTTTCCTTTTGCTACGCCCGCGGTTCTGCACGCCACGACAAAC‑AAAG‑GTGACGCTGAGATCGCTGCCACGGTACCTGATCCCTCCGCGGTACTGGCGACCGATTAAAACATCCCGtt                                                                  <  2:4148506/140‑1 (MQ=255)

                                                                     aaCGGAGAGGCCGGTGAAGAGTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                                  <  2:1596062/140‑1 (MQ=255)

                                                                         gCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGat                                                              >  2:4262132/1‑140 (MQ=255)

                                                                           gATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGataa                                                            <  2:1470937/140‑1 (MQ=255)

                                                                                     aaGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTgg                                                  <  1:1559438/140‑1 (MQ=255)

                                                                                       gATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCa                                                <  2:302888/140‑1 (MQ=255)

                                                                                               tGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCAccc                                        >  1:4718784/1‑140 (MQ=255)

                                                                                                 cTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGc                                      >  2:3089327/1‑140 (MQ=255)

                                                                                                    tCGGTAAGCCCGCGGTTCTGCAAAACCCGAAAAAC‑AAAG‑GTGACGTTGCGAGCGGTGCCACTTTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAAGCTCGTTCGATAATAATCAGTGGCAGGGCAccgtactt                                   >  2:1692543/1‑134 (MQ=255)

                                                                                                    tCGGTAAGCCCGCGGTTCTGCAAAACACGACAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCtt                                   <  1:2237842/140‑1 (MQ=255)

                                                                                                      ggTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGc                                 <  2:4828873/140‑1 (MQ=255)

                                                                                                       gTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGcc                                <  2:4718784/140‑1 (MQ=255)

                                                                                                         aaGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAg                              <  1:4846556/140‑1 (MQ=255)

                                                                                                            ctcgcgGTTCTGCAAAACACGAAAAAT‑AAAG‑GTGACGTTGCGATCGGCGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCTGTCGATAATAATCAGTGGCAGTGCACCCGCCTTTCCAGCaa                           >  2:1162260/3‑140 (MQ=255)

                                                                                                            cccGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCaa                           >  2:1343143/1‑140 (MQ=255)

                                                                                                               gcgGTTCTGCAAACCACGAAAAAC‑AAAGCC‑GACGTTGCGATCGCTGCCACGGTACCTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        <  2:1206776/140‑1 (MQ=255)

                                                                                                               gcgGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        <  1:5139960/140‑1 (MQ=255)

                                                                                                               gcgGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        <  2:5118454/140‑1 (MQ=255)

                                                                                                               gcgGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        >  1:2303994/1‑140 (MQ=255)

                                                                                                               gcgGTTCTGCAAAACACGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        <  2:3303647/140‑1 (MQ=255)

                                                                                                                             cacGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  1:1330217/1‑140 (MQ=255)

                                                                                                                             cacGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  2:1297648/1‑140 (MQ=255)

                                                                                                                             cacGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  1:4537490/1‑140 (MQ=255)

                                                                                                                             cacGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  2:1066122/1‑140 (MQ=255)

                                                                                                                             cacGAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  2:3499596/1‑140 (MQ=255)

                                                                                                                                gAAAAAC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGACGc       >  2:4965827/1‑140 (MQ=255)

                                                                                                                                     aC‑AAAG‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGACGCCGGAt  <  2:2038264/140‑1 (MQ=255)

ACGCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAAC‑AAAG‑G‑GACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGACGCCGGAT  >  NZ_CP025268/1146368‑1146639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`