Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,374,862 (T)8→9 intergenic (+40/‑172) pspE → / → ycjM thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,374,8541.T95.4% 932.9 / 30.0 265intergenic (+32/‑180)pspE/ycjMthiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase
Reads supporting (aligned to +/- strand):  ref base . (8/4);  new base T (128/123);  total (137/128)
Fisher's exact test for biased strand distribution p-value = 3.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 274 total aligned reads displayed.

GACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑G‑‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTG  >  NZ_CP025268/1374716‑1374987
                                                                                                                                             |                                                                                                                                         
gACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑G‑‑t                                                                                                                                         >  1:67511/1‑140 (MQ=255)
       gTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑G‑‑t‑ttttt‑t‑t                                                                                                                               >  1:4748571/1‑140 (MQ=255)
            ggCAAGCAAAAGAGATCCTTAGCGACATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑G‑‑t‑ttttt‑t‑ttctgt                                                                                                                          >  2:4786929/1‑135 (MQ=255)
             gcaagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGGAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTc                                                                                                                          <  1:3634513/140‑1 (MQ=255)
              caagtaaCATAGATTCTTAGCTAGATGGGATATCCCCACGTTGAGAACGCCGGTCGCCTGAAAGACATCGCAGTGCCGAAGGTCAAAGGTTAACAGAATTCTCCAGGTGCGGCGCTTATGCG‑CC‑G‑‑t‑ttttt‑t‑ttctgtta                                                                                                                        <  2:3157188/140‑8 (MQ=255)
              caagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGATCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTca                                                                                                                         <  2:4072082/140‑1 (MQ=255)
              caagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTca                                                                                                                         <  1:2698201/140‑1 (MQ=255)
              caagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTca                                                                                                                         <  1:3162717/140‑1 (MQ=255)
              caagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTca                                                                                                                         <  2:4511680/140‑1 (MQ=255)
               aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTcac                                                                                                                        <  1:555584/140‑1 (MQ=255)
                 gcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCacac                                                                                                                      >  1:2481143/1‑140 (MQ=255)
                 gcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCacac                                                                                                                      <  2:2660895/140‑1 (MQ=255)
                 gcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCacac                                                                                                                      >  1:3842333/1‑140 (MQ=255)
                  cacaaGAGATCCTTAGCGAGATGGGAGATACCCGCGTTGAGAACGCCGGTGGCCTGAACTACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATGCACCATGATCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACAcc                                                                                                                     <  2:1818303/137‑1 (MQ=255)
                  caaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACAcc                                                                                                                     <  2:2344051/140‑1 (MQ=255)
                  caaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACAcc                                                                                                                     >  2:652445/1‑140 (MQ=255)
                   aaaaGATGTCCTGAGCGAGATGGGACATAGCAACGTTGAGAGCGGCGGTGGCCTGAAAGACATCGCAATGCCGAAAGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CA‑G‑‑TGGTTTT‑T‑TCTGTCACACCt                                                                                                                    <  2:2730708/140‑1 (MQ=255)
                   aaaaGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GTTT‑TTTTT‑T‑TCTGTCACAcc                                                                                                                     <  1:4032409/140‑1 (MQ=255)
                         gaTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTa                                                                                                              <  2:3890388/140‑1 (MQ=255)
                          aTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTAc                                                                                                             >  1:5267876/1‑140 (MQ=255)
                           tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACAcc                                                                                                                     >  1:4309253/1‑131 (MQ=255)
                           tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACa                                                                                                            <  2:5076064/140‑1 (MQ=255)
                           tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACa                                                                                                            <  1:1735270/140‑1 (MQ=255)
                           tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTGAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACa                                                                                                            <  2:5331415/140‑1 (MQ=255)
                            ccTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACAt                                                                                                           >  1:4957440/1‑140 (MQ=255)
                             cTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CCGG‑‑T‑TTTTG‑T‑TCTGTCAGACCTTATTTACATc                                                                                                          >  2:4328433/1‑140 (MQ=255)
                                 gCGAGATGGGATATACCCACGTTGAGACCGACGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑G‑‑T‑TTTTTGT‑TCTGTCACACCTTATTTACATCCCCa                                                                                                      <  1:726658/140‑1 (MQ=255)
                                   gacaTGGTAGATACCCCCGTTGAGAACGCCGGTCGCCTGAAAGACATCGCCCTGACGAAGGTCACAGGCTAAAAGTATTCCCCCTGACCGGCGCTTATCCG‑CC‑GC‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATa                                                                                                    <  2:1704818/137‑1 (MQ=255)
                                     gaTGGGATATCCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGa                                                                                                  <  1:1977302/140‑1 (MQ=255)
                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAATAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTTTCACACCTTATTTACATCCCCTTTGat                                                                                                 >  2:4689983/1‑140 (MQ=255)
                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGat                                                                                                 >  2:4305078/1‑140 (MQ=255)
                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGat                                                                                                 >  1:941601/1‑140 (MQ=255)
                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGat                                                                                                 >  1:2586719/1‑140 (MQ=255)
                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGat                                                                                                 >  1:3074176/1‑140 (MQ=255)
                                               aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                        <  1:2304731/140‑1 (MQ=255)
                                               aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                        >  1:4259073/1‑140 (MQ=255)
                                               aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                        >  2:2288271/1‑140 (MQ=255)
                                               aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                        >  1:5227943/1‑140 (MQ=255)
                                                cccACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTc                                                                                       >  2:4946497/1‑140 (MQ=255)
                                                  cACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAGTTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACTTTATTTACATCCCCATAGATTATTTGCGTCAg                                                                                     >  2:219784/1‑140 (MQ=255)
                                                   aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCGTAGATTATTTGCGTCAGc                                                                                    >  1:118079/1‑140 (MQ=255)
                                                   aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGc                                                                                    >  1:3616170/1‑140 (MQ=255)
                                                   aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGc                                                                                    >  1:487622/1‑140 (MQ=255)
                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   >  2:1890979/1‑140 (MQ=255)
                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   >  2:2072323/1‑140 (MQ=255)
                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   >  1:1474726/1‑140 (MQ=255)
                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCGTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   >  1:4078663/1‑140 (MQ=255)
                                                    cGTTGAGAACGCCGGTGGCCTGAAAGAAATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCAGCGCTTATGCA‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   >  2:3923890/1‑140 (MQ=255)
                                                    cGTTGAGAACGCCGGTGGCATGAAAGACATCGCAATGCCAAAAGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCTGCt                                                                                   >  2:162984/1‑140 (MQ=255)
                                                        gagaACGCCGGTGGCCTGAAAGACATCGCACTGCCGCAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTcaca                                                                               >  1:2089689/1‑140 (MQ=255)
                                                        gagaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTcaca                                                                               >  2:4027632/1‑140 (MQ=255)
                                                         agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGACTTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTACTTACATCCCCATAGATTATTTGCGTCAGCTCACaa                                                                              >  2:5246792/1‑140 (MQ=255)
                                                           aaCGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAAt                                                                            >  2:210926/1‑140 (MQ=255)
                                                           aaCGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAAt                                                                            >  1:122703/1‑140 (MQ=255)
                                                            aCGCCGGTGGCCTGAAAGAAATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATa                                                                           <  1:925014/140‑1 (MQ=255)
                                                             cGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GTTT‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATa                                                                           <  1:4803508/140‑1 (MQ=255)
                                                             cGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATAc                                                                          <  1:110819/140‑1 (MQ=255)
                                                              gCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTCAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCCTCGATTATTTGCGTCATCTCACAAATACg                                                                         >  2:1137910/1‑140 (MQ=255)
                                                               ccGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTTACAAATACGc                                                                        >  2:2457508/1‑140 (MQ=255)
                                                               ccGGTGGCCTGAAAGACACCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCTTCAGCTCACAAATACGc                                                                        >  2:1059568/1‑140 (MQ=255)
                                                                  gTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCttt                                                                     <  1:3263254/140‑1 (MQ=255)
                                                                   tGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTGATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCtttt                                                                    >  1:4523980/1‑140 (MQ=255)
                                                                       cTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTccc                                                                <  1:4660586/140‑1 (MQ=255)
                                                                               cATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGAGTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaa                                                        >  2:1430921/1‑140 (MQ=255)
                                                                                aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaa                                                       >  1:509793/1‑140 (MQ=255)
                                                                                  tgCAAAGCCGAAAGTCAAATGTTAAAACAATTCCCCATGAGCGACGCTTATGCG‑CCAC‑‑C‑TCTTT‑C‑TCTGTCACTCTTTATTTACATCCCCATACAATATTTGCATCAACTCACCAAAACGCTTTTTCCCTGGTAAAAAAt                                                     <  2:2904757/139‑1 (MQ=255)
                                                                                  cGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAAt                                                     >  1:5124195/1‑140 (MQ=255)
                                                                                  cGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAAt                                                     >  2:1439178/1‑140 (MQ=255)
                                                                                  cGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACAATGAGCGGCGCTTATGCG‑CG‑GT‑T‑TTTTT‑T‑TATGTCACACCTTATTTACGTCCCCAGTGATTATTTTGGTCAGCTCACAAATACGCTTTTTCCCTGGGAAAAAAt                                                     >  2:3389930/1‑140 (MQ=255)
                                                                                    cAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTa                                                           >  2:4133215/1‑132 (MQ=255)
                                                                                    cAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGa                                                   >  1:137343/1‑140 (MQ=255)
                                                                                       tGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGAttt                                                >  2:3659622/1‑140 (MQ=255)
                                                                                         ccGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCCCACAAATACGATTTTTCCCTGGTAAAAAATGATTTcc                                              >  2:3782748/1‑140 (MQ=255)
                                                                                            aaGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGc                                           >  2:1548829/1‑140 (MQ=255)
                                                                                            aaGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTAACAAATACGCTTTTTCCCTGGTAAAAAATTATTTCCTGc                                           >  2:454207/1‑140 (MQ=255)
                                                                                             aGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCg                                          <  1:2435617/140‑1 (MQ=255)
                                                                                             aGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCg                                          <  1:652445/140‑1 (MQ=255)
                                                                                             aGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCg                                          <  2:1547518/140‑1 (MQ=255)
                                                                                              ggTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGt                                         <  1:369488/140‑1 (MQ=255)
                                                                                              ggTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGt                                         <  1:2685801/140‑1 (MQ=255)
                                                                                              ggTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGt                                         <  2:1603224/140‑1 (MQ=255)
                                                                                                 cAAAGGTTAAAAGGATTCACCATTAAGCGCGCTTATTCGCCC‑T‑‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGAc                                      <  2:3746146/140‑1 (MQ=255)
                                                                                                   aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCg                                          <  1:3382759/134‑1 (MQ=255)
                                                                                                   aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCg                                          >  2:3382727/1‑134 (MQ=255)
                                                                                                   aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTa                                    >  2:2300657/1‑140 (MQ=255)
                                                                                                   aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTa                                    >  2:1655464/1‑140 (MQ=255)
                                                                                                         aaaaGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAAccc                              >  2:5130504/1‑140 (MQ=255)
                                                                                                          aaaGAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCt                             <  2:4930818/140‑1 (MQ=255)
                                                                                                             gAATTCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTtgt                          <  1:156873/140‑1 (MQ=255)
                                                                                                                ttCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt                       >  1:2237772/1‑140 (MQ=255)
                                                                                                                ttCACCATGAGCGGCGCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt                       >  2:2550190/1‑140 (MQ=255)
                                                                                                                ttCACCATGAGCGGCGCTTAAGCG‑CC‑G‑‑T‑TTTTT‑TATCTGTTACACCTTATTGACGTCCCCAACGATTATTTGTTTCTTATCACAAATACGCTTTTTCCCTGGTAAAAAAAGATTTCCTGCGTGACTGATACACTtgtgtt                       >  2:3257960/1‑138 (MQ=255)
                                                                                                                ttCACCATGAGCGGCCCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt                       <  1:3258564/140‑1 (MQ=255)
                                                                                                                            ggcgCTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCCGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTTACAGtt           >  1:3528310/1‑140 (MQ=255)
                                                                                                                                cTTATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAtt       <  2:67511/140‑1 (MQ=255)
                                                                                                                                  tATGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAtttt     <  1:4917228/140‑1 (MQ=255)
                                                                                                                                   aTGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACGTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCCGCGTGACTAAAAACCTTGTGATCAGTTGACAGTTTATTTTc    >  2:1369451/1‑140 (MQ=255)
                                                                                                                                   aTGCG‑CC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                   >  1:2428085/1‑61 (MQ=255)
                                                                                                                                     gcg‑cC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTg  <  2:2567642/140‑1 (MQ=255)
                                                                                                                                     gcg‑cC‑GT‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTg  <  2:2279980/140‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                         
GACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCG‑CC‑G‑‑T‑TTTTT‑T‑TCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTG  >  NZ_CP025268/1374716‑1374987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑