BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,660,178	+C	coding (314/351 nt)	ynfA ←	YnfA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,660,174	1	.	C	97.1%	662.8 / 10.5	208	coding (318/351 nt)	ynfA	YnfA family protein
Reads supporting (aligned to +/- strand): ref base . (4/2); new base C (81/121); total (85/123)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

TCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑C‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAG  >  NZ_CP025268/1660035‑1660307

 cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                          >  1:5133164/1‑140 (MQ=255)

  gAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c                                                                                                                                        >  1:3484963/1‑140 (MQ=255)

  gAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c                                                                                                                                        >  1:2908028/1‑140 (MQ=255)

  gAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c                                                                                                                                        >  1:2298743/1‑140 (MQ=255)

    aaaaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTTACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c‑c‑c                                                                                                                                    <  2:4964087/140‑1 (MQ=255)

    aaaaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATCCCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTCTGTTCCTACGTGCG‑c‑c‑c‑c                                                                                                                                    <  1:3236444/140‑1 (MQ=255)

      aaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c‑c‑cca                                                                                                                                  <  1:4380783/140‑3 (MQ=255)

         gCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c‑c‑ccagcc                                                                                                                               <  1:2530714/140‑6 (MQ=255)

         gCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c‑c‑ccagcc                                                                                                                               <  1:3826422/140‑6 (MQ=255)

         gCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c‑c‑ccagcc                                                                                                                               >  2:5192067/1‑135 (MQ=255)

         gCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c‑c‑ccagcc                                                                                                                               >  2:1270341/1‑135 (MQ=255)

          ccGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGccc                                                                                                                              >  2:1336281/1‑140 (MQ=255)

                tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGcaaca                                                                                                                        <  1:484635/140‑1 (MQ=255)

                tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGcaaca                                                                                                                        <  1:176643/140‑1 (MQ=255)

                    cacaAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGa                                                                                                                    >  2:3335910/1‑140 (MQ=255)

                       aaaaaaaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCa                                                                                                                 <  1:3636622/140‑1 (MQ=255)

                       aaaaaaaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCa                                                                                                                 <  1:77717/140‑1 (MQ=255)

                                       ttGAGCTGTGAATGGTTGACAACAGATGAAATAGAATCCCTTTTGCCAGCTGACCCTTCCACTTATCTTATTGATAAAATGGCTTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAgcgc                                                                                                 <  2:1386524/140‑1 (MQ=255)

                                        cgccatgtaAATGCTTGACAAAAGCAGACACAGAATACATCTTAAAAGCCGACACCTCCTCTAATCTTACTGATAAAACGGATTAAAGTTCCTACGTGCG‑C‑CAC‑A‑AGCCCGCAACAACGATCAACATGCCGCAAAGCGCa                                                                                                <  2:161287/135‑1 (MQ=255)

                                         gcgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATGTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGGGCaa                                                                                               >  2:3832303/1‑140 (MQ=255)

                                          cgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAAt                                                                                              >  1:4507921/1‑140 (MQ=255)

                                          cgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAAt                                                                                              >  1:1892912/1‑140 (MQ=255)

                                            cTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCa                                                                                            >  1:141298/1‑140 (MQ=255)

                                              gtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAAc                                                                                          >  1:2148974/1‑140 (MQ=255)

                                              gtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAAc                                                                                          >  1:5241751/1‑140 (MQ=255)

                                               tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                         >  1:834100/1‑140 (MQ=255)

                                               tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                         >  1:5012742/1‑140 (MQ=255)

                                                   tGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAcc                                                                                     <  2:1631754/140‑1 (MQ=255)

                                                     gTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCg                                                                                   >  1:3732428/1‑140 (MQ=255)

                                                       tGACAACAGATGAAACAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                 <  2:3821604/140‑1 (MQ=255)

                                                       tGACAAAAGATGAAATAGCATACCTTTTGTCAGCTGACCCTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGAAACAATGATCAACATGCCGCAAAGCCCAATCAACGAACCCGTc                                                                                 <  2:560459/140‑1 (MQ=255)

                                                       tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                 <  2:3667471/140‑1 (MQ=255)

                                                       tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                 <  1:1672661/140‑1 (MQ=255)

                                                       tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                 <  1:1454882/140‑1 (MQ=255)

                                                       tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                 <  1:133731/140‑1 (MQ=255)

                                                       tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCACTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCACCGAACCCGTc                                                                                 <  2:626005/140‑1 (MQ=255)

                                                       tGACAAAAGATGAAATAGAATACCCTTTGTCAGCTGACACTTCCTCTTATCTTATTGAAAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                 <  2:1981245/140‑1 (MQ=255)

                                                         aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                               >  2:915553/1‑140 (MQ=255)

                                                              aGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCAt                                                                          >  1:1595858/1‑140 (MQ=255)

                                                               gATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCATACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATa                                                                         <  2:4934205/140‑1 (MQ=255)

                                                               aaTGAAATAGAATCCCTTCTGCCAGCTGACATTTCCTCTTATCTTATTGATAAAATGGATCTATGTCACTACGTGCGCC‑C‑C‑C‑AGCCCGCAAGAATGAGCAACATGCCGCAAAGCGCAATCATCGCACCCGACCAGTCATa                                                                         <  2:4364332/139‑1 (MQ=255)

                                                                aTGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATaa                                                                        >  1:2629361/1‑140 (MQ=255)

                                                                    aaTAGAATCCGTTTTGTCAGCTGACACTTCCTATTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑C‑C‑CTC‑AGCCCGCAACAATGATCAACATGCAGCAAAGCGCAACCAACGCACCCGTACAGTCATAAagag                                                                    >  2:1166015/1‑140 (MQ=255)

                                                                    aaTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                 >  1:200521/1‑127 (MQ=255)

                                                                     aTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                                   <  2:203766/140‑1 (MQ=255)

                                                                     aTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                                   <  1:964952/140‑1 (MQ=255)

                                                                     aTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                                   <  1:3429365/140‑1 (MQ=255)

                                                                     aTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                                   <  1:415619/140‑1 (MQ=255)

                                                                         aaTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTACTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACGCGTCCAGTCATACAGAGTCAGt                                                               >  2:2812154/1‑140 (MQ=255)

                                                                          aTACCTTTAGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACATTCGCC‑C‑C‑C‑AGCCCGCAACAATAATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGtt                                                              >  2:2207730/1‑140 (MQ=255)

                                                                            aCCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTc                                                            <  2:4655000/140‑1 (MQ=255)

                                                                               ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAACATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAATGCACCCGTCCAGTCATAAAGAGCCAGTTTCACg                                                         >  2:3720677/1‑140 (MQ=255)

                                                                               ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                         <  1:5230712/140‑1 (MQ=255)

                                                                               ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                         <  1:2706988/140‑1 (MQ=255)

                                                                                tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                        <  1:3001368/140‑1 (MQ=255)

                                                                                tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTAATGTTGCTACGTGCG‑C‑C‑C‑CAAGCCCGCAACAATGATCAAGATGCCGCAAAGCGCAATAAACGCACCCGTCCAGTCATAAAGAGTCAGCTTAACGc                                                        >  2:4546563/1‑140 (MQ=255)

                                                                                  tGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCa                                                      >  2:1047279/1‑140 (MQ=255)

                                                                                       gCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcac                                                 >  2:1323146/1‑140 (MQ=255)

                                                                                        cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcaca                                                >  2:2387206/1‑140 (MQ=255)

                                                                                           acacTTCCTCGTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcg                                             <  2:1856733/140‑1 (MQ=255)

                                                                                            cacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgc                                            >  1:4851333/1‑140 (MQ=255)

                                                                                                 ccTCTTATCTTATTGATCAAATGGATCTATCTTCCTACGTGCGCC‑C‑C‑C‑CGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                       <  2:1805769/140‑1 (MQ=255)

                                                                                                 ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGCGTCAGTTTCACGCCATCCACAACGCGCAGc                                       <  2:291966/140‑1 (MQ=255)

                                                                                                 ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                       <  1:4032977/140‑1 (MQ=255)

                                                                                                 ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                       <  1:307533/140‑1 (MQ=255)

                                                                                                 ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                       <  1:3741798/140‑1 (MQ=255)

                                                                                                 ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                       <  2:1172614/140‑1 (MQ=255)

                                                                                                 ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                       <  2:2524605/140‑1 (MQ=255)

                                                                                                  ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGcc                                      >  2:1490383/1‑140 (MQ=255)

                                                                                                  ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                       >  1:3562707/1‑139 (MQ=255)

                                                                                                  ctctTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                       <  2:3562707/139‑1 (MQ=255)

                                                                                                           tatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                             <  1:3919786/140‑1 (MQ=255)

                                                                                                           tatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                             <  2:2690547/140‑1 (MQ=255)

                                                                                                           tatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAAAAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                             <  1:664361/140‑1 (MQ=255)

                                                                                                             ttGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                           >  1:1130693/1‑140 (MQ=255)

                                                                                                             ttGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                           >  1:3582203/1‑140 (MQ=255)

                                                                                                             ttGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGGCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                           >  2:5327294/1‑140 (MQ=255)

                                                                                                                aTAAAATGTATTTATGCTCCGACGAGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAATGCTCAATCAACGCACCCGTCCAGTCATGAAGAGTCCGTTTCACGCCATCCACAACGCGGAGCCTCATGCACGCCGTg                        <  2:3497186/140‑1 (MQ=255)

                                                                                                                aTAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                        <  2:23238/140‑1 (MQ=255)

                                                                                                                aTAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                        <  1:1009966/140‑1 (MQ=255)

                                                                                                                aTAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                        <  1:915553/140‑1 (MQ=255)

                                                                                                                  aaaaTGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCa                      <  2:2087973/140‑1 (MQ=255)

                                                                                                                   aaaTGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAg                     <  1:4613629/140‑1 (MQ=255)

                                                                                                                    aaTGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGa                    >  2:4095894/1‑140 (MQ=255)

                                                                                                                    aaTGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGa                    >  2:4096052/1‑140 (MQ=255)

                                                                                                                          tttATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                                   <  2:4998659/87‑1 (MQ=255)

                                                                                                                          tttATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                                   >  1:4998497/1‑87 (MQ=255)

                                                                                                                            tATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg            <  1:4297437/140‑1 (MQ=255)

                                                                                                                            tATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg            <  2:5138328/140‑1 (MQ=255)

                                                                                                                            tATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg            <  1:2188385/140‑1 (MQ=255)

                                                                                                                            tATGTTCATACGTGCGCC‑C‑C‑C‑AGCACGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg            <  2:851470/140‑1 (MQ=255)

                                                                                                                             aTGTTCCTACGTGCG‑CAC‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGc           <  2:4021782/140‑1 (MQ=255)

                                                                                                                                  cctACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGccacca      >  2:3403843/3‑140 (MQ=255)

                                                                                                                                  cctACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGccacca      >  2:1231308/3‑140 (MQ=255)

                                                                                                                                   ctACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCGACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCAt     >  2:2847337/2‑140 (MQ=255)

                                                                                                                                   ctACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCAt     >  2:112522/2‑140 (MQ=255)

                                                                                                                                   ctACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCAt     >  1:2079765/2‑140 (MQ=255)

                                                                                                                                     aCGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATaa   >  1:264966/1‑140 (MQ=255)

                                                                                                                                      cGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAg  <  1:1793504/140‑1 (MQ=255)

                                                                                                                                      cGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAg  <  1:4816435/140‑1 (MQ=255)

TCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑C‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAG  >  NZ_CP025268/1660035‑1660307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`