BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,690,360	(C)_5→6	pseudogene (1047/1403 nt)	fumC ←	class II fumarate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,690,355	1	.	C	96.4%	1233.6 / 35.3	385	pseudogene (1052/1403 nt)	fumC	class II fumarate hydratase
Reads supporting (aligned to +/- strand): ref base . (5/9); new base C (151/220); total (156/229)
Fisher's exact test for biased strand distribution p-value = 7.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 402 total aligned reads displayed.

TCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCAC  >  NZ_CP025268/1690217‑1690487

tCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAATCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAgc‑g‑c                                                                                                                                         <  2:4614334/140‑1 (MQ=255)

tCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAgc‑g‑c                                                                                                                                         <  2:1926525/140‑1 (MQ=255)

tCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAgc‑g‑c                                                                                                                                         <  1:168489/140‑1 (MQ=255)

   cTCACGATTCTGTTCAATACCCACTGCGCAGTGTTTGTTAAATCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGCAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑c‑c‑c‑c                                                                                                                                   <  2:4300200/140‑1 (MQ=255)

   cTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑c‑c‑c‑c                                                                                                                                   <  1:1841402/140‑1 (MQ=255)

   cTCACCATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑c‑c‑c‑c                                                                                                                                   <  2:4702901/140‑1 (MQ=255)

           tCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTg                                                                                                                           <  2:4926394/140‑1 (MQ=255)

             ggTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGAt                                                                                                                         >  2:30577/1‑140 (MQ=255)

                 cAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGcc                                                                                                                     <  2:3088171/140‑1 (MQ=255)

                    tACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCAcg                                                                                                                  >  2:1130302/1‑140 (MQ=255)

                    tACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCAcg                                                                                                                  >  2:441933/1‑140 (MQ=255)

                       ccACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCAcgtcg                                                                                                               <  1:5147945/140‑1 (MQ=255)

                         aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCAcgtcgt                                                                                                              <  1:1481961/139‑1 (MQ=255)

                         aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGtt                                                                                                             >  2:3649324/1‑140 (MQ=255)

                         aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGtt                                                                                                             >  1:884770/1‑140 (MQ=255)

                         aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGtt                                                                                                             >  2:4144431/1‑140 (MQ=255)

                         aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGtt                                                                                                             >  2:3361734/1‑140 (MQ=255)

                         aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGtt                                                                                                             >  2:1060895/1‑140 (MQ=255)

                         aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGAAAGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGtt                                                                                                             >  1:4440473/1‑140 (MQ=255)

                         aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGAGTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGATCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTTATCGCCACGTCGtt                                                                                                             >  2:2514985/1‑140 (MQ=255)

                         aCTGCGCAGTGTTTGTTAAAACGTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTGAAAGTTACCGGAAGC‑G‑C‑C‑C‑CCGGTGTTGATGGCCACGTCGtt                                                                                                             >  2:886204/1‑140 (MQ=255)

                            gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTccc                                                                                                          >  1:549338/1‑140 (MQ=255)

                            gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTccc                                                                                                          >  2:1378755/1‑140 (MQ=255)

                            gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTccc                                                                                                          >  1:2163716/1‑140 (MQ=255)

                            gcgcAGTGTTTGGTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTCCCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCTTTccc                                                                                                          >  2:2146156/1‑140 (MQ=255)

                            gcgcAGTGTTGGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGGAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGGTCAAAGTTAAAGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTccc                                                                                                          >  2:3840413/1‑140 (MQ=255)

                               cAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCAt                                                                                                       <  1:1286943/140‑1 (MQ=255)

                                 gtgtTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCa                                                                                                     <  1:278429/140‑1 (MQ=255)

                                 gtgtTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCa                                                                                                     <  2:4851351/140‑1 (MQ=255)

                                 gtgtTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCa                                                                                                     >  2:242730/1‑140 (MQ=255)

                                  tgtttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCAc                                                                                                    >  1:3745291/1‑140 (MQ=255)

                                    tttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCt                                                                                                  >  1:4901800/1‑140 (MQ=255)

                                       gttAAAACTTTCCATGCGATCCGCCAGCAAGCGCACCGATTGCAGGAAATGGTGGATCACCATTGGACGGACGCCGGTTAGTGAAGAGCTACCGGAAGC‑G‑CGC‑C‑CA‑ATGTTGAGCGCAAGGTCCTGCCCCATccaccgca                                                                                               >  2:102930/1‑133 (MQ=255)

                                       gttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGAc                                                                                               >  1:5276322/1‑140 (MQ=255)

                                         tAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                             <  1:2618446/140‑1 (MQ=255)

                                         tAAAACTTTCCATGCCATCTGCCAGCAACCGCACCGATTGCAGGAAATTGTGGATCAACATTGGACGGAACACGTTCATTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                             <  1:295235/140‑1 (MQ=255)

                                            aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                          >  1:4503579/1‑140 (MQ=255)

                                            aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                          >  1:1004646/1‑140 (MQ=255)

                                            aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                          >  2:2003447/1‑140 (MQ=255)

                                            aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                          >  2:4486981/1‑140 (MQ=255)

                                               tttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                       <  1:3495536/140‑1 (MQ=255)

                                               tttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                       <  1:3670470/140‑1 (MQ=255)

                                                ttCTATGCCATATGCCAGCAACCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTCCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCa                                                                                      <  1:4328863/140‑1 (MQ=255)

                                                ttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCa                                                                                      <  1:2499117/140‑1 (MQ=255)

                                                    aTGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGt                                                                                  <  1:492952/140‑1 (MQ=255)

                                                    aTGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACCTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑CTGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGt                                                                                  <  2:4716543/140‑1 (MQ=255)

                                                      gCCATCTGCGAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGCAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                <  1:2988242/140‑1 (MQ=255)

                                                      gCCATCTGCCAGCAATCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                <  2:1187010/140‑1 (MQ=255)

                                                      gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAACGTTACCGGAAGCGG‑C‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                <  2:2710532/140‑1 (MQ=255)

                                                      gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                <  2:1572593/140‑1 (MQ=255)

                                                      gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                <  2:626018/140‑1 (MQ=255)

                                                      gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                <  2:767859/140‑1 (MQ=255)

                                                      gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                <  1:1084585/140‑1 (MQ=255)

                                                      gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                <  1:2028101/140‑1 (MQ=255)

                                                      gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                <  1:4093766/140‑1 (MQ=255)

                                                      gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGAAGAGCATGGTTa                                                                                <  2:5290338/140‑1 (MQ=255)

                                                      gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGAGCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTGCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                <  2:1309126/140‑1 (MQ=255)

                                                      gCCATCTGCCAGAAAGCGCACCGAGTGTAGGAAATTGTGGCTCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCAACTGACAGCAGAGCATGGTTa                                                                                <  1:1380364/140‑1 (MQ=255)

                                                        cATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAAt                                                                              <  1:97424/140‑1 (MQ=255)

                                                           cTGCCAGCAAGCTCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGcc                                                                           <  1:4571686/140‑1 (MQ=255)

                                                               cAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcac                                                                       <  1:816809/140‑1 (MQ=255)

                                                               cAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcac                                                                       <  1:610283/140‑1 (MQ=255)

                                                                aGCACGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGTTCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                      <  1:236430/140‑1 (MQ=255)

                                                                aGCAAGCGCAGCGGTTGCAGGAAATTGTGGATCACCCTTGGCCGCAAGGCGTCCAGTTCAAAGTTACCGGAAGC‑G‑C‑CAC‑CC‑ATGTTGATCGCCACGTCGTTCCCCCTCACCTGACAGCAGCGCATGGTTAATGCCTcaca                                                                      <  1:3065933/140‑1 (MQ=255)

                                                                aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCCAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                      <  1:2856730/140‑1 (MQ=255)

                                                                aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                      <  1:858958/140‑1 (MQ=255)

                                                                aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                      <  1:2319680/140‑1 (MQ=255)

                                                                aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                      <  1:3445833/140‑1 (MQ=255)

                                                                aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                      <  2:2801345/140‑1 (MQ=255)

                                                                aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                      <  1:5308580/140‑1 (MQ=255)

                                                                aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                      <  2:1672279/140‑1 (MQ=255)

                                                                aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                      <  1:705161/140‑1 (MQ=255)

                                                                aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                      <  1:665411/140‑1 (MQ=255)

                                                                aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCAGTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                      <  1:1655629/140‑1 (MQ=255)

                                                                aGCAAGCGCACCGATTGCAGGAAATTGTCGATCACCATTGGCCGCAAGCCGTTCAGTTCAAAGCCACCGGAATC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                      <  2:40763/140‑1 (MQ=255)

                                                                aGCAAGCGCACCGATTGAAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGCTCAAAGTTACCGGAAGC‑G‑C‑C‑CACC‑ATGTTGATCGCCACGTCGTTCCCCATCCCCTGACAGCAGAGCATGGTTAATGCCTcaaa                                                                      <  2:2948512/140‑3 (MQ=255)

                                                                    cgcgcACCGATTGCAGGAAATTATGGATCACCATTGGACGGAACACGTTCAGATCACAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCCCGTCGGTCCCCATCCCCTGGCAGCAGGGCACGGTTAGTGCCTCACACtgt                                                                  >  2:2257552/2‑140 (MQ=255)

                                                                     gcgcACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACtgtg                                                                 <  1:760867/140‑1 (MQ=255)

                                                                          ccGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCGGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTggg                                                            >  1:4196057/1‑140 (MQ=255)

                                                                            gATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGtt                                                          <  2:1465476/140‑1 (MQ=255)

                                                                                     aaaTTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTccc                                                 <  2:3314064/140‑1 (MQ=255)

                                                                                     aaaTTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTccc                                                 <  1:4842000/140‑1 (MQ=255)

                                                                                        ttGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCgg                                              <  1:2918496/140‑1 (MQ=255)

                                                                                           tgGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                <  2:3509266/103‑1 (MQ=255)

                                                                                                   cATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGc                                   >  2:5037680/1‑140 (MQ=255)

                                                                                                     ttGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTg                                 >  1:3865068/1‑140 (MQ=255)

                                                                                                     ttGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTg                                 >  1:3345795/1‑140 (MQ=255)

                                                                                                        ggcGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGccc                              >  2:4272134/3‑140 (MQ=255)

                                                                                                          cGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCgg                            >  1:4747369/1‑140 (MQ=255)

                                                                                                             aaGAGGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                         >  2:4335227/1‑140 (MQ=255)

                                                                                                             aaGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                         >  2:1806025/1‑140 (MQ=255)

                                                                                                             aaGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                         >  1:383357/1‑140 (MQ=255)

                                                                                                             aaGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                         >  2:3603976/1‑140 (MQ=255)

                                                                                                             aaGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                         >  1:4412897/1‑140 (MQ=255)

                                                                                                             aaGACGTTCAGTTCAAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                         >  2:2731452/1‑140 (MQ=255)

                                                                                                                   ttcagttcaAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTc                   >  1:2131349/1‑140 (MQ=255)

                                                                                                                    tcagttcaAAGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTcc                  <  1:4977530/140‑1 (MQ=255)

                                                                                                                             aGTTACCGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTgag         >  1:1425560/1‑140 (MQ=255)

                                                                                                                                  ccGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTc    >  2:3569291/1‑140 (MQ=255)

                                                                                                                                  ccGGAAGC‑GCC‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTc    >  2:2668391/1‑140 (MQ=255)

TCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑C‑C‑C‑CC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCAC  >  NZ_CP025268/1690217‑1690487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`