BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,701,269	+A	intergenic (‑380/+10)	uidA ← / ← uidR	beta‑glucuronidase/DNA‑binding transcriptional regulator UidR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,701,265	1	.	A	95.5%	1241.6 / 40.4	337	intergenic (‑376/+14)	uidA/uidR	beta‑glucuronidase/DNA‑binding transcriptional regulator UidR
Reads supporting (aligned to +/- strand): ref base . (8/7); new base A (163/159); total (171/166)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 342 total aligned reads displayed.

GCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTA  >  NZ_CP025268/1701127‑1701397

     ttGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑aa‑a‑aag                                                                                                                                 >  2:3136792/1‑138 (MQ=255)

          aaCGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AA‑A‑a‑‑agagaga                                                                                                                          >  1:2542608/1‑140 (MQ=255)

          aaCGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AA‑A‑a‑‑agagaga                                                                                                                          >  2:3185858/1‑140 (MQ=255)

               tAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATc                                                                                                                       >  2:3566131/1‑140 (MQ=255)

                   ctctCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGa                                                                                                                   <  1:4526670/140‑1 (MQ=255)

                    tctcATGACATATGTCTACAGTGAATATTTTGGGGGGAATACTCCGAAGCCGGGAAGGATAATTTTTGAACAGAGGAATTACGATATGAAACGCGGGCACAGTTATAACCAGCCAGGCGAAA‑A‑A‑GAGAGAGAATAAGGaa                                                                                                                  >  2:220417/1‑139 (MQ=255)

                    tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGACGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                  <  2:1818369/140‑1 (MQ=255)

                    tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                  <  1:869720/140‑1 (MQ=255)

                    tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                  <  2:2640675/140‑1 (MQ=255)

                    tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                  <  1:2342037/140‑1 (MQ=255)

                    tctcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                  <  1:3382253/140‑1 (MQ=255)

                      tcATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGc                                                                                                                <  2:3716197/140‑1 (MQ=255)

                       cATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCg                                                                                                               >  1:1358032/1‑140 (MQ=255)

                       cATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCg                                                                                                               >  1:3256230/1‑140 (MQ=255)

                       cATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGATATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAAACAGGATGCg                                                                                                               >  2:1537876/1‑140 (MQ=255)

                        aTGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCgg                                                                                                              >  2:1133054/1‑140 (MQ=255)

                         tGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGt                                                                                                             <  1:4828020/140‑1 (MQ=255)

                              tatGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGACGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGa                                                                                                        >  2:936489/1‑140 (MQ=255)

                              tatGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGACGGTAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATGAGCAGCGCGCTGAAA‑A‑A‑GGGAGAGAATCAGGATGGGGTTAAGa                                                                                                        >  2:4892555/1‑140 (MQ=255)

                                tGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCATAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGTATGCGGTTAAGATa                                                                                                      >  2:506534/1‑140 (MQ=255)

                                tGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATa                                                                                                      >  1:5006529/1‑140 (MQ=255)

                                tGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATa                                                                                                      >  2:1345924/1‑140 (MQ=255)

                                      cAGTGCATATTGTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTAGCACAGAGAAATTACGATATGAAACGCCGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAAGACGCGGATAAGATACCGCCa                                                                                                >  2:4305538/1‑140 (MQ=255)

                                      cAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCa                                                                                                >  1:4951434/1‑140 (MQ=255)

                                      cAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCa                                                                                                >  2:2282276/1‑140 (MQ=255)

                                      cAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCa                                                                                                >  1:921640/1‑140 (MQ=255)

                                      cAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCa                                                                                                >  1:5192138/1‑140 (MQ=255)

                                      cAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTAGCACCAGCCCGCCG‑AAGA‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCa                                                                                                >  1:5286816/1‑140 (MQ=255)

                                       aGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCaa                                                                                               >  2:5342931/1‑140 (MQ=255)

                                         tGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatc                                                                                             >  1:5068264/1‑140 (MQ=255)

                                           aaTATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGCCACACTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatcat                                                                                           >  1:4107608/1‑140 (MQ=255)

                                             tatTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATcg                                                                                         >  2:3127095/1‑140 (MQ=255)

                                             tatTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATcg                                                                                         <  1:3055829/140‑1 (MQ=255)

                                                   ggggggAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTAGCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTa                                                                                   >  2:2827151/1‑140 (MQ=255)

                                                     ggggAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTGAt                                                                                 >  2:1806618/1‑140 (MQ=255)

                                                     ggggAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAAt                                                                                 >  2:2128508/1‑140 (MQ=255)

                                                     ggggAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAAt                                                                                 >  2:3847161/1‑140 (MQ=255)

                                                       ggAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATcc                                                                               >  2:2517724/1‑140 (MQ=255)

                                                        gAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATccc                                                                              <  2:4533105/140‑1 (MQ=255)

                                                                    gCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAacgac                                                                  <  2:3961820/140‑1 (MQ=255)

                                                                          aaGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCa                                                            <  1:2507871/140‑1 (MQ=255)

                                                                          aaGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCa                                                            <  1:2872573/140‑1 (MQ=255)

                                                                                aaTTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGcc                                                      <  1:2236155/140‑1 (MQ=255)

                                                                                       gCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCGCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGAGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCtt                                               >  2:4647561/1‑140 (MQ=255)

                                                                                       gCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                  >  1:1416992/1‑73 (MQ=255)

                                                                                       gCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGAt                                                                                                                  <  2:1416992/73‑1 (MQ=255)

                                                                                       gCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCtt                                               >  1:3606602/1‑140 (MQ=255)

                                                                                       gCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCAGCCG‑AA‑AGA‑GTGAGAGGATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAAGGACGTTGCACGCGCCAGGTCtt                                               >  2:4200463/1‑140 (MQ=255)

                                                                                        cacaGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTc                                              <  1:3543932/140‑1 (MQ=255)

                                                                                        cacaGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTc                                              <  2:1506626/140‑1 (MQ=255)

                                                                                                  ttACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                    <  1:2890943/140‑1 (MQ=255)

                                                                                                  ttACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                    <  1:1856596/140‑1 (MQ=255)

                                                                                                  ttACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                    <  1:561885/140‑1 (MQ=255)

                                                                                                  ttACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                    <  2:1996586/140‑1 (MQ=255)

                                                                                                  ttACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGata                                    <  2:2917577/140‑1 (MQ=255)

                                                                                                    aCGATATGACACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATc                                  <  1:1236259/140‑1 (MQ=255)

                                                                                                    aCGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATc                                  <  2:2068459/140‑1 (MQ=255)

                                                                                                    aCGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AA‑AGA‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTACGCTTCGATATc                                  <  2:5295431/140‑1 (MQ=255)

                                                                                                       atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                               <  2:4280462/140‑1 (MQ=255)

                                                                                                       atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                               <  1:740624/140‑1 (MQ=255)

                                                                                                       atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                               <  1:66526/140‑1 (MQ=255)

                                                                                                       atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                               <  2:3410616/140‑1 (MQ=255)

                                                                                                       atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                               >  2:2990875/1‑140 (MQ=255)

                                                                                                       atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                               >  1:3938187/1‑140 (MQ=255)

                                                                                                       atatGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATACCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                               <  2:2871406/140‑1 (MQ=255)

                                                                                                       atatGAAACGCGGGCACAGTTAGCACCAGCCCGCGGAAA‑A‑A‑GAGCGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCCTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGc                               <  2:2328792/140‑1 (MQ=255)

                                                                                                            aaaCGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTa                          <  2:5122822/140‑1 (MQ=255)

                                                                                                            aaaCGCGGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTa                          <  1:2755672/140‑1 (MQ=255)

                                                                                                                gcgGGCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACAcc                      <  2:3515235/140‑1 (MQ=255)

                                                                                                                  gggCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTc                                              >  2:715687/1‑114 (MQ=255)

                                                                                                                  gggCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTa                          <  2:5202603/134‑1 (MQ=255)

                                                                                                                  gggCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGt                    >  1:4298696/1‑140 (MQ=255)

                                                                                                                  gggCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGt                    <  1:374507/140‑1 (MQ=255)

                                                                                                                  gggCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGt                    >  1:4385750/1‑140 (MQ=255)

                                                                                                                  gggCACAGTTATCACAAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGt                    <  2:1909237/140‑1 (MQ=255)

                                                                                                                   ggCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTa                   >  2:4122709/1‑140 (MQ=255)

                                                                                                                    gCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAg                  >  1:4682393/1‑140 (MQ=255)

                                                                                                                    gCACAGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAg                  >  2:4687208/1‑140 (MQ=255)

                                                                                                                     cacaGTTATCACAAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAgg                 <  1:2632413/140‑1 (MQ=255)

                                                                                                                      acaGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGt                >  1:4179893/1‑140 (MQ=255)

                                                                                                                       caGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTc               <  2:2814090/140‑1 (MQ=255)

                                                                                                                       caGTTATCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTc               <  1:2145796/140‑1 (MQ=255)

                                                                                                                          aggtCACCAGCCCGCCG‑AA‑A‑AGGAGGGAGAACCAGGACGCGGACAAGATACCGCCACTCATCGCGCGTAACCCCTGAGCAAACGACGCGCCACGCGCCAGGTCCTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGa            >  2:4797767/4‑140 (MQ=255)

                                                                                                                            aTCACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATc          <  2:307607/140‑1 (MQ=255)

                                                                                                                              cACCAGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCca        <  2:166116/140‑1 (MQ=255)

                                                                                                                                  aGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCcagcag    >  2:3373746/1‑140 (MQ=255)

                                                                                                                                  aGCCCGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCcagcag    >  1:1748643/1‑140 (MQ=255)

                                                                                                                                    cccGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATTGGCCAGTACACCGTAGGTCAGATCCAGCAGTa  >  1:146753/1‑140 (MQ=255)

                                                                                                                                    cccGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCTGCCAGTACACCGTAGGTCAGATCCAGCAGTa  >  1:2036955/1‑140 (MQ=255)

                                                                                                                                    cccGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGt                    <  1:3222077/122‑1 (MQ=255)

                                                                                                                                    cccGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa  >  1:2353097/1‑140 (MQ=255)

                                                                                                                                    cccGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa  >  2:4458582/1‑140 (MQ=255)

                                                                                                                                    cccGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa  >  1:3177259/1‑140 (MQ=255)

                                                                                                                                    cccGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa  >  2:3549150/1‑140 (MQ=255)

                                                                                                                                    cccGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa  >  2:610894/1‑140 (MQ=255)

                                                                                                                                    cccGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa  >  1:4565355/1‑140 (MQ=255)

                                                                                                                                    cccGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa  >  1:1109135/1‑140 (MQ=255)

                                                                                                                                    cccGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTAACGCGCCAGGTCTTCCGCTTCGATATGGGCCAGTACACCGTAGGTCAGATCCAGCAGTa  <  2:699236/140‑1 (MQ=255)

                                                                                                                                    cccGCCGAAA‑A‑A‑GAGAGAGAATCAGGATGCGCTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTa  >  2:3252654/1‑140 (MQ=255)

                                                                                                                                    cccGCCGAAA‑A‑A‑GAGAGACAATCAGGAAGCGGTTAAGATACCGCGAATCATCGAGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTGTTCCGCTTCGATAGCGGCCAGTACACCGTAGGGCAGATCCAGCAGTa  >  2:863708/1‑140 (MQ=255)

GCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCG‑AA‑A‑A‑GAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCGCGCGTAATCCCTGAGCAAACGACGCTTCACGCGCCAGGTCTTCCGCTTCGATATCGGCCAGTACACCGTAGGTCAGATCCAGCAGTA  >  NZ_CP025268/1701127‑1701397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`