BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,033,768	(C)_5→6	pseudogene (190/920 nt)	yedA →	drug/metabolite exporter YedA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,033,763	1	.	C	97.2%	924.5 / 16.6	285	pseudogene (185/920 nt)	yedA	drug/metabolite exporter YedA
Reads supporting (aligned to +/- strand): ref base . (7/1); new base C (126/150); total (134/151)
Fisher's exact test for biased strand distribution p-value = 2.77e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 320 total aligned reads displayed.

GTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑T‑C‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGCC  >  NZ_CP025268/2033626‑2033897

gTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAATCTGGCCTCCGTTAATGATGGCGGGCGTTCGATGCCTGGCAGCCGGTAGTTTATTGCTGGCATTTTTTCTACTGCGCGGACACAAAC‑T‑c‑c                                                                                                                                       >  1:4473716/1‑140 (MQ=255)

gTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑T‑c‑c                                                                                                                                       >  1:875950/1‑140 (MQ=255)

gTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑T‑c‑c                                                                                                                                       >  2:1253993/1‑140 (MQ=255)

gTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑T‑c‑c                                                                                                                                       <  2:3241222/140‑1 (MQ=255)

 tataTCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑T‑c‑c‑c                                                                                                                                     <  1:798332/140‑1 (MQ=255)

 tataTCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑T‑c‑c‑c                                                                                                                                     <  1:2470794/140‑1 (MQ=255)

  atatCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑T‑c‑c‑cc                                                                                                                                    >  2:765886/1‑140 (MQ=255)

  atatCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑T‑c‑c‑cc                                                                                                                                    >  2:4559735/1‑140 (MQ=255)

        tttGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑T‑c‑c‑ccccgcta                                                                                                                              >  2:2948775/1‑135 (MQ=255)

         ttGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTAc                                                                                                                              <  2:3038199/140‑1 (MQ=255)

         ttGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCGGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTAc                                                                                                                              <  2:648611/140‑1 (MQ=255)

           ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGt                                                                                                                            >  1:3494999/1‑140 (MQ=255)

           ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGt                                                                                                                            >  1:3849625/1‑140 (MQ=255)

           ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGt                                                                                                                            >  2:2838087/1‑140 (MQ=255)

           ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGt                                                                                                                            >  2:4227777/1‑140 (MQ=255)

           ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGt                                                                                                                            >  1:1485619/1‑140 (MQ=255)

           ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGt                                                                                                                            >  2:5213014/1‑140 (MQ=255)

           ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGt                                                                                                                            >  2:851627/1‑140 (MQ=255)

           ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGt                                                                                                                            >  1:1102076/1‑140 (MQ=255)

           ggggCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAACGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCCACTGCGCGGACACAAAC‑T‑CAC‑CCCGCTACGt                                                                                                                            >  2:4177878/1‑140 (MQ=255)

           ggggCTCAACCTATGTTGTCATTCGGATTGGCGTAGAAAGCTGGCCTCCGTTAACGATGGCGGGCGGTCGATTCCTGGCAGCCGGCATTTTACTGCCGGCATTTTTGCTACTGCGGGGCCAAAAAC‑C‑C‑CACCCGCTACgg                                                                                                                            >  2:940295/1‑139 (MQ=255)

           ggggCCCTACCTACTTTGTCATTCGGATTGGCGTGGCCAGCTGGCCTCCGTTAAGGATGGCGAGCGTTCGCTTTCTCGCAGCCCGTCTTGTTTTTCTGGCATTTTGGCTCCTGCGCGGACACAAACTT‑C‑C‑CCCGCTACGt                                                                                                                            >  2:2108622/1‑140 (MQ=255)

              gCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCg                                                                                                                         <  1:1820499/140‑1 (MQ=255)

                 cAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATGTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCgctg                                                                                                                      >  2:1860935/1‑140 (MQ=255)

                 cAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATGTTATTGCGGGCATTTTTGCGACTGCGCGGACACAAAC‑TCC‑C‑CCCGGTACGTCCgctg                                                                                                                      >  2:2782985/1‑140 (MQ=255)

                  aaCCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCgctgc                                                                                                                     >  1:495779/1‑140 (MQ=255)

                   aCCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCgctgct                                                                                                                    >  1:2657213/1‑140 (MQ=255)

                        ttttGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATg                                                                                                               <  2:2576862/140‑1 (MQ=255)

                            gTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCcgc                                                                                                           <  2:2616621/140‑1 (MQ=255)

                            gTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCcgc                                                                                                           >  2:4296801/1‑140 (MQ=255)

                            gTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCcgc                                                                                                           <  1:1578235/140‑1 (MQ=255)

                             tCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCcgcg                                                                                                          <  2:5040100/140‑1 (MQ=255)

                             tCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCcgcg                                                                                                          <  2:3260377/140‑1 (MQ=255)

                             tCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATCGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCcgcg                                                                                                          <  2:1664588/140‑1 (MQ=255)

                              cATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCAATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCgcgc                                                                                                         >  2:4145460/1‑140 (MQ=255)

                                  cGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCgctgct                                                                                                                    >  1:4405460/1‑125 (MQ=255)

                                  cGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCgctgct                                                                                                                    <  2:4405460/125‑1 (MQ=255)

                                  cGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGa                                                                                                      >  2:4905914/1‑139 (MQ=255)

                                  cGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑GCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGAt                                                                                                     <  2:2372611/140‑1 (MQ=255)

                                  cGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGGATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCACTGCCGCGCTGAt                                                                                                     >  1:1986228/1‑140 (MQ=255)

                                     aTTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTgg                                                                                                  <  2:2230921/140‑1 (MQ=255)

                                     aTTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTgg                                                                                                  <  1:4995678/140‑1 (MQ=255)

                                       tGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGcc                                                                                                <  1:2583544/140‑1 (MQ=255)

                                               aaaGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGGAGCCGGTATTTTATTGCGGGCATTTTGGCTACTGCGCGGACACAAACGC‑C‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCGGATTTGCCTGTTATTg                                                                                        >  2:1153012/1‑140 (MQ=255)

                                                   cTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctgg                                                                                    >  2:574657/1‑140 (MQ=255)

                                                   cTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctgg                                                                                    <  2:3526969/140‑1 (MQ=255)

                                                   cTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctgg                                                                                    >  2:4706364/1‑140 (MQ=255)

                                                   cTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctgg                                                                                    <  2:5174370/140‑1 (MQ=255)

                                                      gCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctggctg                                                                                 >  2:1087764/1‑140 (MQ=255)

                                                       ccTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGt                                                                                <  1:3736765/140‑1 (MQ=255)

                                                       ccTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGt                                                                                <  1:3233691/140‑1 (MQ=255)

                                                          ccGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCgg                                                                             <  1:889624/140‑1 (MQ=255)

                                                            gTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCCTTTTTGCTACTGCGCGGACACAACC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTa                                                                           <  1:535510/140‑1 (MQ=255)

                                                            gTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTa                                                                           <  2:1488801/140‑1 (MQ=255)

                                                            gTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCAACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTa                                                                           <  1:2948775/140‑1 (MQ=255)

                                                             ttAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTaa                                                                          >  1:1935685/1‑140 (MQ=255)

                                                                     ggcgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATgg                                                                  >  1:4376669/1‑140 (MQ=255)

                                                                     ggcgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATgg                                                                  >  1:2904622/1‑140 (MQ=255)

                                                                       cgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTg                                                                >  2:1338363/1‑140 (MQ=255)

                                                                       cgggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTg                                                                >  1:3001923/1‑140 (MQ=255)

                                                                        gggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGa                                                               <  2:3697746/140‑1 (MQ=255)

                                                                         ggcgTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGGGAc                                                              >  2:1975698/1‑140 (MQ=255)

                                                                             ttCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGtt                                                          >  2:1553486/1‑140 (MQ=255)

                                                                                gATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGcc                                                       <  1:3834002/140‑1 (MQ=255)

                                                                                            ccGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTgctgg                                                                                    >  1:1682702/1‑99 (MQ=255)

                                                                                             cGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATg                                          <  1:4224549/140‑1 (MQ=255)

                                                                                              ggTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATgg                                         >  2:4524270/1‑139 (MQ=255)

                                                                                                  ttttATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGttcct                                     <  1:5214598/140‑1 (MQ=255)

                                                                                                          cTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCAt                             <  1:1260475/140‑1 (MQ=255)

                                                                                                          cTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCAt                             >  1:2490263/1‑140 (MQ=255)

                                                                                                          cTGGCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCAt                             >  1:4833385/1‑140 (MQ=255)

                                                                                                            ggCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATcg                           >  2:3076522/1‑140 (MQ=255)

                                                                                                            ggCATTTTTGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATcg                           <  1:3333432/140‑1 (MQ=255)

                                                                                                                tttttGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATcgccgc                       <  1:4145460/140‑1 (MQ=255)

                                                                                                                 ttttGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCgccgcc                      >  1:3994559/1‑140 (MQ=255)

                                                                                                                    tGCTACTGCGCGGACACAGAC‑TCC‑C‑CCCGCTACGCCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTa                   <  2:3404148/140‑1 (MQ=255)

                                                                                                                    tGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTa                   <  1:1846191/140‑1 (MQ=255)

                                                                                                                    tGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTa                   <  1:287046/140‑1 (MQ=255)

                                                                                                                    tGCTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTa                   <  2:2458188/140‑1 (MQ=255)

                                                                                                                      cTACTGCGCGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGt                 <  2:1039614/140‑1 (MQ=255)

                                                                                                                           gcgcGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCAt                             <  1:1508047/123‑1 (MQ=255)

                                                                                                                           gcgcGGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCAt                             >  2:1508047/1‑123 (MQ=255)

                                                                                                                            cgcgGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGc           >  1:4585565/1‑140 (MQ=255)

                                                                                                                            cgcgGACACAAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGGGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGc           <  1:2578353/140‑1 (MQ=255)

                                                                                                                              ccgACACAATC‑TCC‑C‑CCCGCTCCGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAGTTTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCaa         <  2:2673600/138‑1 (MQ=255)

                                                                                                                                   acaAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTg    <  1:1338363/140‑1 (MQ=255)

                                                                                                                                   acaAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTg    <  1:5163560/140‑1 (MQ=255)

                                                                                                                                   acaAAC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTg    <  1:974628/140‑1 (MQ=255)

                                                                                                                                     aaaC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  1:1013404/140‑1 (MQ=255)

                                                                                                                                     aaaC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  1:1453067/140‑1 (MQ=255)

                                                                                                                                     aaaC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  1:1222504/140‑1 (MQ=255)

                                                                                                                                     aaaC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  1:3129537/140‑1 (MQ=255)

                                                                                                                                     aaaC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  1:3301707/140‑1 (MQ=255)

                                                                                                                                     aaaC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  2:4737059/140‑1 (MQ=255)

                                                                                                                                     aaaC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  1:3441928/140‑1 (MQ=255)

                                                                                                                                     aaaC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  2:3935147/140‑1 (MQ=255)

                                                                                                                                     aaaC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  1:597480/140‑1 (MQ=255)

                                                                                                                                     aaaC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  1:2122747/140‑1 (MQ=255)

                                                                                                                                     aaaC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  2:1409657/140‑1 (MQ=255)

                                                                                                                                     aaaC‑TCC‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGGGACGGTTGCCGAACATCAAAATGTTCCTTCCCGCATCGCCGCCGTAGTGGTTGCAACCGTGcc  <  2:4098122/140‑1 (MQ=255)

GTATATCATTTGGGGCTCAACCTATTTTGTCATTCGGATTGGCGTGGAAAGCTGGCCTCCGTTAATGATGGCGGGCGTTCGATTCCTGGCAGCCGGTATTTTATTGCTGGCATTTTTGCTACTGCGCGGACACAAAC‑T‑C‑C‑CCCGCTACGTCCGCTGCTCAATGCCGCGCTGATTGGCCTGTTATTGCTGGCTGTCGGTAATGGCATGGTGACGGTTGCCGAACATCAAAATGTTCCTTCCGGCATCGCCGCCGTAGTGGTTGCAACCGTGCC  >  NZ_CP025268/2033626‑2033897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`