BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,218,374	(C)_5→6	pseudogene (306/1685 nt)	btsS ←	two‑component regulatory system sensor histidine kinase BtsS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,218,369	1	.	C	96.4%	890.2 / 23.0	276	pseudogene (311/1685 nt)	btsS	two‑component regulatory system sensor histidine kinase BtsS
Reads supporting (aligned to +/- strand): ref base . (5/5); new base C (139/126); total (144/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

GCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑CCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAC  >  NZ_CP025268/2218232‑2218503

 cgacgaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑ccc                                                                                                                                       >  1:3697858/1‑140 (MQ=255)

  gacgaACGTGACTGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑ccc‑c                                                                                                                                     <  2:3762034/140‑1 (MQ=255)

     gatcGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑ccc‑ccca                                                                                                                                  <  1:4585093/137‑3 (MQ=255)

     gaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑ccc‑ccca                                                                                                                                  >  1:1415233/1‑138 (MQ=255)

     gaACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑ccc‑ccca                                                                                                                                  >  2:3235159/1‑138 (MQ=255)

      aaCGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑ccc‑cccat                                                                                                                                 >  2:319126/1‑137 (MQ=255)

            aCGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAata                                                                                                                           <  1:1059482/140‑1 (MQ=255)

            aCGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAata                                                                                                                           <  1:319126/140‑1 (MQ=255)

            aCGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAata                                                                                                                           <  1:2908452/140‑1 (MQ=255)

             cGGCACCGGCGGTAATGGGGTTCAAGACTATATCAGTGCGCCCACAGCGGATCAAGATGCTGTGTACCAAGCCCCCGAGTCATCCTTCAAAGATGGTCGAGATCAAGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATAGAatat                                                                                                                          <  2:2529229/140‑1 (MQ=255)

             cGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAatat                                                                                                                          <  1:912185/140‑1 (MQ=255)

                cACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCATGATGCTGTGTATAAGGCCACCGAGTAATCCTTGAACGATGGTCGAGATTATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGa                                                                                                                       >  1:2733938/1‑140 (MQ=255)

                 aCCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGAt                                                                                                                      >  2:1360905/1‑140 (MQ=255)

                     gcggTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTaa                                                                                                                  >  2:966118/1‑140 (MQ=255)

                          aaTGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAgccgc                                                                                                             >  1:2068841/1‑140 (MQ=255)

                             ggggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCgg                                                                                                          <  1:1239467/140‑1 (MQ=255)

                             ggggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCgg                                                                                                          <  1:283946/140‑1 (MQ=255)

                             ggggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCgg                                                                                                          <  2:1269361/140‑1 (MQ=255)

                             ggggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCgg                                                                                                          <  1:4091867/140‑1 (MQ=255)

                             ggggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCgg                                                                                                          <  2:4933810/140‑1 (MQ=255)

                              gggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGt                                                                                                         <  2:1233025/140‑1 (MQ=255)

                               ggTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTGAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTc                                                                                                        >  1:5286905/1‑140 (MQ=255)

                                gTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCa                                                                                                       >  1:2642411/1‑140 (MQ=255)

                                gTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCa                                                                                                       >  1:2995378/1‑140 (MQ=255)

                                gTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCa                                                                                                       >  1:4256818/1‑140 (MQ=255)

                                   aaaGACTTTATCAGTGCGCCCGCGGCGGATCAGGAGGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATAGCGATGTAAGCCGCCGGTCAGcc                                                                                                    >  1:27026/1‑140 (MQ=255)

                                    aaGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACGTAACGCGGTCAT‑GCCCC‑CC‑ATCGACTATCGATGTAAGCCGACGGTCAGccc                                                                                                   >  2:3857487/1‑140 (MQ=255)

                                       aCTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAAc                                                                                                <  2:3495235/140‑1 (MQ=255)

                                         tttATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACCATGGTCGAGGCCATGCAACTTAACGCGGTCATCC‑CCC‑CC‑ATCGAATACCGATATAAGCAGCCGCTAAGCCCacaca                                                                                              >  2:2698553/1‑136 (MQ=255)

                                          ttATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAg                                                                                             >  2:2208229/1‑140 (MQ=255)

                                           tATCAGTGCGCCCGCGGCGGATCAGGCTGCTGTGTCCCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑CCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGc                                                                                            <  2:3821139/140‑1 (MQ=255)

                                           tATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGc                                                                                            >  2:1468487/1‑140 (MQ=255)

                                           tATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGc                                                                                            >  2:527471/1‑140 (MQ=255)

                                            aTCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGcc                                                                                           <  1:3489649/140‑1 (MQ=255)

                                                 tGCGCCCGCGGCGTATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCATCAACGATTGTCGAGAGCATGCAACTTAACTCGGTCAT‑G‑CCC‑CCAATCGAATATCGATGAAAGCCGCCGGACAGCCCAACCAGGCCAcgg                                                                                      >  2:1927075/1‑138 (MQ=255)

                                                 tGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcg                                                                                      >  1:3972392/1‑140 (MQ=255)

                                                 tGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcg                                                                                      >  2:4504458/1‑140 (MQ=255)

                                                 tGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcg                                                                                      >  2:4716730/1‑140 (MQ=255)

                                                   cgcCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcgac                                                                                    >  1:3236723/1‑140 (MQ=255)

                                                   cgcCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑ccc‑cccatc                                                                                                                                >  2:4392401/1‑92 (MQ=255)

                                                   cgcCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑ccc‑cccatc                                                                                                                                <  1:4392401/96‑5 (MQ=255)

                                                     cccGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACcgacga                                                                                  >  1:4043761/1‑140 (MQ=255)

                                                          ggcggATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCGTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑TTCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccgga                                                                             <  2:395549/140‑1 (MQ=255)

                                                            cggATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACgaccggacc                                                                           <  2:21077/140‑1 (MQ=255)

                                                                      tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 <  1:3366911/140‑1 (MQ=255)

                                                                      tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 <  1:1866195/140‑1 (MQ=255)

                                                                      tGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 <  1:975233/140‑1 (MQ=255)

                                                                      ggCTGTGTACCAGGCTACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTCACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAg                                                                 <  2:1144300/139‑1 (MQ=255)

                                                                        cTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCCCCGACGACCGGACCGCCGAGTAAgcc                                                               <  1:1774217/140‑1 (MQ=255)

                                                                         tgtgTACCAGGCCACCGAGTATTCCTTCAACGATGGTCGAGATCAGGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAAAATCGACGTAAGCCCCCGGGCAGCCCAACCAGCCGACCGACGACCGGAACCCCGGGAAAgcag                                                              >  2:4444067/1‑138 (MQ=255)

                                                                         tgtgTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccg                                                              >  1:2489797/1‑140 (MQ=255)

                                                                         tgtgTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccg                                                              >  1:3656580/1‑140 (MQ=255)

                                                                         tgtgTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCACACCGACGACCGGACCGCCGAGAAAgccg                                                              >  2:2408995/1‑140 (MQ=255)

                                                                           tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCCGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccgcc                                                            >  1:5214946/1‑140 (MQ=255)

                                                                           tgtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAgccgcc                                                            <  1:633377/140‑1 (MQ=255)

                                                                            gtACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑CCCTCC‑ATCGAATATCAATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGccc                                                           <  2:2328424/140‑1 (MQ=255)

                                                                             tACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCATCC‑CCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCa                                                          <  2:712822/140‑1 (MQ=255)

                                                                              aCCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTGAGCCGCCGGGCAGCCCAACCAGCCCACCGACACCCGGACCGCCCAGTACGCCGCCCAt                                                         >  2:4829068/1‑140 (MQ=255)

                                                                              aCCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCAt                                                         >  2:2620959/1‑140 (MQ=255)

                                                                                  ggCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTAcc                                                     >  2:2452388/1‑140 (MQ=255)

                                                                                   gCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcg                                                    <  1:4812327/140‑1 (MQ=255)

                                                                                   gCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcg                                                    >  1:3138369/1‑140 (MQ=255)

                                                                                   gCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcg                                                    >  1:4920863/1‑140 (MQ=255)

                                                                                     cACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcgcg                                                  >  1:3950736/1‑140 (MQ=255)

                                                                                     cACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACcgcg                                                  >  1:475836/1‑140 (MQ=255)

                                                                                       ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCGCAATACCGCGcc                                                >  2:1653824/1‑140 (MQ=255)

                                                                                       ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGcc                                                >  1:1007511/1‑140 (MQ=255)

                                                                                       ccGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGcc                                                <  2:563336/140‑1 (MQ=255)

                                                                                       ccGAGTAATCCTTCAACGATGGTAGAGATCATGCAACTTAACGCGGTCAC‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGcc                                                <  1:4900981/140‑1 (MQ=255)

                                                                                          aGTAATCCTTCAACGATGGTCGAGCTCATGCACCTTAACGCGATCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCCGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTAt                                             <  2:293058/140‑1 (MQ=255)

                                                                                          aGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTAt                                             >  2:1586458/1‑140 (MQ=255)

                                                                                            tAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCg                                           >  1:86716/1‑140 (MQ=255)

                                                                                             aaTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGc                                          >  1:3063561/1‑140 (MQ=255)

                                                                                              aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  1:2301239/140‑1 (MQ=255)

                                                                                              aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  1:4716730/140‑1 (MQ=255)

                                                                                              aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  1:5152379/140‑1 (MQ=255)

                                                                                              aTCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCa                                         <  1:519607/140‑1 (MQ=255)

                                                                                               tCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGCAAGCCGCCCATTACCGCGCCGATCGCAc                                        >  2:402457/1‑140 (MQ=255)

                                                                                                 cTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACgg                                      >  1:2217605/1‑140 (MQ=255)

                                                                                                    cAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTa                                   >  1:2806434/1‑140 (MQ=255)

                                                                                                      aCGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTAtt                                 <  1:558479/140‑1 (MQ=255)

                                                                                                        gATGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTgg                               <  1:1586458/140‑1 (MQ=255)

                                                                                                          tGGTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCa                             >  1:1992566/1‑140 (MQ=255)

                                                                                                            gTCGAGATCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGAACGGGTATTGGCAAt                           >  2:1784225/1‑140 (MQ=255)

                                                                                                                agaTCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGaa                       <  2:3697858/140‑1 (MQ=255)

                                                                                                                 gaTCATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAAt                      <  1:1526545/140‑1 (MQ=255)

                                                                                                                    cATGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGt                   <  2:661989/140‑1 (MQ=255)

                                                                                                                      tGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa                 >  2:4235833/1‑140 (MQ=255)

                                                                                                                      tGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa                 >  2:2884404/1‑140 (MQ=255)

                                                                                                                      tGCAACTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa                 >  1:1926945/1‑140 (MQ=255)

                                                                                                                      tGCAACTTAACGCGGGCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCa                 <  1:2007856/140‑1 (MQ=255)

                                                                                                                           cTTAACGCTGTCAT‑GCCCC‑CC‑ATCGAATATCGATTTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGTACCGCCGAGTAAGCCGCCCATTACAGCGCCTATCGCACGGGGATTGGCAATATAATGTTCAAtgtt            >  2:4637636/1‑139 (MQ=255)

                                                                                                                           cTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAAtgtg            <  2:1506745/140‑1 (MQ=255)

                                                                                                                           cTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAAtgtg            <  1:2039422/140‑1 (MQ=255)

                                                                                                                           cTTAACGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAAtgtg            <  1:1841181/140‑1 (MQ=255)

                                                                                                                              aaCGCGGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCaa         <  2:1979294/140‑1 (MQ=255)

                                                                                                                                cgcgGTCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAAcc       >  2:3453891/1‑140 (MQ=255)

                                                                                                                                   ggTCAT‑GCCCC‑CC‑CTCGAATCTCGATGTACGCCGCCGGTCAGCCCAACCCGCCCACCGACGACCGGACAGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCaa    <  2:1416739/140‑1 (MQ=255)

                                                                                                                                   ggTCAA‑G‑CCCACC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACAACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCaa    <  2:4088626/140‑1 (MQ=255)

                                                                                                                                     tCAT‑GCCCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAc  >  2:807846/1‑140 (MQ=255)

GCGACGAACGTGACGGCACCGGCGGTAATGGGGTTAAAGACTTTATCAGTGCGCCCGCGGCGGATCAGGATGCTGTGTACCAGGCCACCGAGTAATCCTTCAACGATGGTCGAGATCATGCAACTTAACGCGGTCAT‑G‑CCC‑CC‑ATCGAATATCGATGTAAGCCGCCGGTCAGCCCAACCAGCCCACCGACGACCGGACCGCCGAGTAAGCCGCCCATTACCGCGCCTATCGCACGGGTATTGGCAATAGAATCGTCAATGTGCAACCCAAAC  >  NZ_CP025268/2218232‑2218503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`